Restriction map for
pFN21AB8145
(Circular) (Six-base) MAPSORT of: pFN21AB8145.seq Check: 1996 from: 1 to: 5629
pFN21AB8145 5629 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3439 278
Size: 53 83 186 936 1903 2468
Fragments arranged by size:
2468 1903 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2212 2972 1937
Size: 275 760 4594
Fragments arranged by size:
4594 760 275
Acc65I G'GTAC_C
Cuts at: 2950 2950
Size: 5629
AclI AA'CG_TT
Cuts at: 1563 3756 4129 1563
Size: 2193 373 3063
Fragments arranged by size:
3063 2193 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3692 5076 5344 1632
Size: 2060 1384 268 1917
Fragments arranged by size:
2060 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5432
AflIII A'CryG_T
Cuts at: 1203 2337 4528 5505 1203
Size: 1134 2191 977 1327
Fragments arranged by size:
2191 1327 1134 977
AgeI A'CCGG_T
Cuts at: 4460 4460
Size: 5629
AhdI GACnn_n'nnGTC
Cuts at: 4358 4358
Size: 5629
AleI CACnn'nnGTG
Cuts at: 1166 2909 1166
Size: 1743 3886
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5629
AlwNI CAG_nnn'CTG
Cuts at: 2240 3372 4944 2240
Size: 1132 1572 2925
Fragments arranged by size:
2925 1572 1132
ApaLI G'TGCA_C
Cuts at: 3686 4842 3686
Size: 1156 4473
ApoI r'AATT_y
Cuts at: 1493 2477 2938 3164 3316 5425 1493
Size: 984 461 226 152 2109 1697
Fragments arranged by size:
2109 1697 984 461 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4183 160
Size: 872 3151 1606
Fragments arranged by size:
3151 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5629
AvaI C'yCGr_G
Cuts at: 1870 1943 2063 2954 1870
Size: 73 120 891 4545
Fragments arranged by size:
4545 891 120 73
BamHI G'GATC_C
Cuts at: 2959 3326 2959
Size: 367 5262
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2537 2681 2693 2903 2950
Size: 325 217 627 750 144 12 210 47
Cuts at: 2950 4405 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 750 627 325 217 210 144
47 12
BanII G_rGCy'C
Cuts at: 1391 2064 2948 1391
Size: 673 884 4072
Fragments arranged by size:
4072 884 673
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4658
BbvCI CC'TCA_GC
Cuts at: 2109 2109
Size: 5629
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3820 3854 1903
Size: 34 1883 34 3678
Fragments arranged by size:
3678 1883 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3521 4731 3521
Size: 1210 4419
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5629
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4240 136
Size: 107 122 71 631 605 2568 1525
Fragments arranged by size:
2568 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5624 5624
Size: 5629
BlpI GC'TnA_GC
Cuts at: 3046 3046
Size: 5629
Bme1580I G_kGCm'C
Cuts at: 1790 2684 2906 3690 4846 1790
Size: 894 222 784 1156 2573
Fragments arranged by size:
2573 1156 894 784 222
BmgBI CAC'GTC
Cuts at: 1318 2712 1318
Size: 1394 4235
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2783 3360 4318
Size: 416 53 83 399 24 1366 577 958
Cuts at: 4318 442
Size: 1753
Fragments arranged by size:
1753 1366 958 577 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5629
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4289 1239
Size: 3050 2579
Bpu10I CC'TnA_GC
Cuts at: 2109 2109
Size: 5629
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3754 4619 4917 3754
Size: 865 298 4466
Fragments arranged by size:
4466 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2286 4292 882
Size: 617 787 2006 2219
Fragments arranged by size:
2219 2006 787 617
BsaAI yAC'GTr
Cuts at: 493 1204 3420 493
Size: 711 2216 2702
Fragments arranged by size:
2702 2216 711
BsaBI GATnn'nnATC
Cuts at: 2128 5623 2128
Size: 3495 2134
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3436 3818 4453 275
Size: 53 83 186 936 1903 382 635 1451
Fragments arranged by size:
1903 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4062 4460 4734 4881 5292 4062
Size: 398 274 147 411 4399
Fragments arranged by size:
4399 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5404
Fragments arranged by size:
5404 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2126 1186
Size: 940 4689
BseYI C'CCAG_C
Cuts at: 2805 4832 5328 2805
Size: 2027 496 3106
Fragments arranged by size:
3106 2027 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5056
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3079 3840 4868 1540
Size: 275 186 1078 761 1028 2301
Fragments arranged by size:
2301 1078 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3690 3775 4846 3690
Size: 85 1071 4473
Fragments arranged by size:
4473 1071 85
BsmI GAATG_Cn'
Cuts at: 3140 3233 3140
Size: 93 5536
BsmBI CGTCTCn'nnnn_
Cuts at: 2037 2037
Size: 5629
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2064 2684 2906 2948 3690 3775 4846
Size: 399 274 620 222 42 742 85 1071
Cuts at: 4846 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 620 399 274 222 85
42
BspEI T'CCGG_A
Cuts at: 5292 5292
Size: 5629
BspHI T'CATG_A
Cuts at: 3518 5248 3518
Size: 1730 3899
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2917 2984 844
Size: 2073 67 3489
Fragments arranged by size:
3489 2073 67
BsrBI CCG'CTC
Cuts at: 1394 1823 3516 1394
Size: 429 1693 3507
Fragments arranged by size:
3507 1693 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4124 4298 65
Size: 1183 2876 174 1396
Fragments arranged by size:
2876 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4273 4460 1700
Size: 110 2463 187 2869
Fragments arranged by size:
2869 2463 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5629
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5629
BssSI C'ACGA_G
Cuts at: 2306 3689 4701 2306
Size: 1383 1012 3234
Fragments arranged by size:
3234 1383 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5629
BstBI TT'CG_AA
Cuts at: 4433 4433
Size: 5629
BstEII G'GTnAC_C
Cuts at: 1184 2854 1184
Size: 1670 3959
BstYI r'GATC_y
Cuts at: 1972 2358 2406 2959 3326 3711 3728 5180 5266
Size: 386 48 553 367 385 17 1452 86
Cuts at: 5266 5278 5624 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 553 386 385 367 346 86
48 17 12
Bsu36I CC'TnA_GG
Cuts at: 2250 2250
Size: 5629
BtgI C'CryG_G
Cuts at: 513 1065 2004 2569 513
Size: 552 939 565 3573
Fragments arranged by size:
3573 939 565 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4890
Fragments arranged by size:
4890 683 56
BtsI GCAGTG_nn'
Cuts at: 2727 3145 3939 3959 2727
Size: 418 794 20 4397
Fragments arranged by size:
4397 794 418 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5629
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5594
DraI TTT'AAA
Cuts at: 2933 2933
Size: 5629
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4636 809
Size: 507 3320 1802
Fragments arranged by size:
3320 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3076 3965 5594
Size: 54 1425 50 275 18 1246 889 1629
Cuts at: 5594 8
Size: 43
Fragments arranged by size:
1629 1425 1246 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3076 1537
Size: 275 1264 4090
Fragments arranged by size:
4090 1264 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2753 3559 1353
Size: 1400 806 3423
Fragments arranged by size:
3423 1400 806
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2136 4216 4590 4736 387
Size: 1064 138 547 2080 374 146 1280
Fragments arranged by size:
2080 1280 1064 547 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3692 4289 5076 5344 1239
Size: 393 2060 597 787 268 1524
Fragments arranged by size:
2060 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2938 3316 2938
Size: 378 5251
FspI TGC'GCA
Cuts at: 4135 4135
Size: 5629
HaeII r_GCGC'y
Cuts at: 1678 2868 4776 1678
Size: 1190 1908 2531
Fragments arranged by size:
2531 1908 1190
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2051 2083 4270 4302 4344
Size: 32 135 32 479 32 2187 32 42
Cuts at: 4344 4376 1373
Size: 32 2626
Fragments arranged by size:
2626 2187 479 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2973 3219 669
Size: 988 281 1035 246 3079
Fragments arranged by size:
3079 1035 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2414 748
Size: 1666 3963
HpaI GTT'AAC
Cuts at: 3219 3219
Size: 5629
KpnI G_GTAC'C
Cuts at: 2954 2954
Size: 5629
MfeI C'AATT_G
Cuts at: 3228 3228
Size: 5629
MluI A'CGCG_T
Cuts at: 5505 5505
Size: 5629
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2199 4743 4927 1305
Size: 894 2544 184 2007
Fragments arranged by size:
2544 2007 894 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3807
Fragments arranged by size:
3807 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2909 3587 3946 4105 518
Size: 648 1743 678 359 159 2042
Fragments arranged by size:
2042 1743 678 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2741 2843 2871 3045 3722 4870 5115 1606
Size: 1135 102 28 174 677 1148 245 2120
Fragments arranged by size:
2120 1148 1135 677 245 174 102 28
NcoI C'CATG_G
Cuts at: 513 1065 2004 2569 513
Size: 552 939 565 3573
Fragments arranged by size:
3573 939 565 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5629
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5629
NotI GC'GGCC_GC
Cuts at: 3076 3076
Size: 5629
NspI r_CATG'y
Cuts at: 1132 1229 2341 2987 4532 1132
Size: 97 1112 646 1545 2229
Fragments arranged by size:
2229 1545 1112 646 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5629
PciI A'CATG_T
Cuts at: 2337 4528 2337
Size: 2191 3438
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5629
PmeI GTTT'AAAC
Cuts at: 2933 2933
Size: 5629
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3698 3730 5211 5243 3698
Size: 32 1481 32 4084
Fragments arranged by size:
4084 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2885 1613
Size: 1272 4357
PsiI TTA'TAA
Cuts at: 3199 3199
Size: 5629
PstI C_TGCA'G
Cuts at: 830 2981 830
Size: 2151 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5629
PvuII CAG'CTG
Cuts at: 2741 2843 2741
Size: 102 5527
SalI G'TCGA_C
Cuts at: 1936 2971 1936
Size: 1035 4594
SbfI CC_TGCA'GG
Cuts at: 2981 2981
Size: 5629
ScaI AGT'ACT
Cuts at: 3877 5589 3877
Size: 1712 3917
SfcI C'TryA_G
Cuts at: 826 1045 2977 4112 4793 4984 826
Size: 219 1932 1135 681 191 1471
Fragments arranged by size:
1932 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5629
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5629
SmaI CCC'GGG
Cuts at: 1872 2956 1872
Size: 1084 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3733 4634 4896 820
Size: 197 926 1790 901 262 1553
Fragments arranged by size:
1790 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3420 493
Size: 2927 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5629
SphI G_CATG'C
Cuts at: 1132 2987 1132
Size: 1855 3774
SspI AAT'ATT
Cuts at: 5 52 3553 5
Size: 47 3501 2081
Fragments arranged by size:
3501 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5629
StyI C'CwwG_G
Cuts at: 513 1065 2004 2569 2607 3068 513
Size: 552 939 565 38 461 3074
Fragments arranged by size:
3074 939 565 552 461 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3656 3673 3826 4011 4456 325
Size: 1255 2076 17 153 185 445 1498
Fragments arranged by size:
2076 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3875 5587 96
Size: 274 80 33 51 1443 1898 1712 138
Fragments arranged by size:
1898 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5589 5621 5589
Size: 32 5597
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5629
XbaI T'CTAG_A
Cuts at: 2965 2965
Size: 5629
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5629
XmaI C'CCGG_G
Cuts at: 1870 2954 1870
Size: 1084 4545
XmnI GAAnn'nnTTC
Cuts at: 2328 3758 2328
Size: 1430 4199
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3437 276
Size: 53 83 186 936 1903 2468
Fragments arranged by size:
2468 1903 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI