Restriction map for
pFN21AB8143
(Circular) (Six-base) MAPSORT of: pFN21AB8143.seq Check: 6375 from: 1 to: 5923
pFN21AB8143 5923 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2282 2282
Size: 5923
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3733 278
Size: 53 83 186 936 2197 2468
Fragments arranged by size:
2468 2197 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3266 1937
Size: 1329 4594
Acc65I G'GTAC_C
Cuts at: 2183 3244 2183
Size: 1061 4862
AclI AA'CG_TT
Cuts at: 1563 4050 4423 1563
Size: 2487 373 3063
Fragments arranged by size:
3063 2487 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2609 3986 5370 5638 1632
Size: 977 1377 1384 268 1917
Fragments arranged by size:
1917 1384 1377 977 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5726
AflIII A'CryG_T
Cuts at: 1203 2484 4822 5799 1203
Size: 1281 2338 977 1327
Fragments arranged by size:
2338 1327 1281 977
AgeI A'CCGG_T
Cuts at: 4754 4754
Size: 5923
AhdI GACnn_n'nnGTC
Cuts at: 4652 4652
Size: 5923
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5923
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5923
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2684 2716 2684
Size: 32 5891
AlwNI CAG_nnn'CTG
Cuts at: 2073 2530 3666 5238 2073
Size: 457 1136 1572 2758
Fragments arranged by size:
2758 1572 1136 457
ApaI G_GGCC'C
Cuts at: 2871 2871
Size: 5923
ApaLI G'TGCA_C
Cuts at: 3980 5136 3980
Size: 1156 4767
ApoI r'AATT_y
Cuts at: 1493 3232 3458 3610 5719 1493
Size: 1739 226 152 2109 1697
Fragments arranged by size:
2109 1739 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4477 160
Size: 872 3445 1606
Fragments arranged by size:
3445 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5923
AvaI C'yCGr_G
Cuts at: 1870 1943 2870 3056 3074 3089 3248 1870
Size: 73 927 186 18 15 159 4545
Fragments arranged by size:
4545 927 186 159 73 18 15
BamHI G'GATC_C
Cuts at: 3253 3620 3253
Size: 367 5556
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2183 2534 2738 2745 3244
Size: 325 217 627 396 351 204 7 499
Cuts at: 3244 4699 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 499 396 351 325 217
204 7
BanII G_rGCy'C
Cuts at: 1391 2814 2871 3081 3242 1391
Size: 1423 57 210 161 4072
Fragments arranged by size:
4072 1423 210 161 57
BbeI G_GCGC'C
Cuts at: 2538 2742 2749 2538
Size: 204 7 5712
Fragments arranged by size:
5712 204 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3112 928
Size: 971 1213 3739
Fragments arranged by size:
3739 1213 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4114 4148 1903
Size: 34 2177 34 3678
Fragments arranged by size:
3678 2177 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3815 5025 3815
Size: 1210 4713
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5923
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4534 136
Size: 107 122 71 631 605 2862 1525
Fragments arranged by size:
2862 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5918 5918
Size: 5923
BlpI GC'TnA_GC
Cuts at: 2726 3340 2726
Size: 614 5309
Bme1580I G_kGCm'C
Cuts at: 1790 2871 3984 5140 1790
Size: 1081 1113 1156 2573
Fragments arranged by size:
2573 1156 1113 1081
BmgBI CAC'GTC
Cuts at: 1318 2080 1318
Size: 762 5161
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2415 3654 4612
Size: 416 53 83 399 24 998 1239 958
Cuts at: 4612 442
Size: 1753
Fragments arranged by size:
1753 1239 998 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5923
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2119 2600 2819 3005 4583 1239
Size: 880 481 219 186 1578 2579
Fragments arranged by size:
2579 1578 880 481 219 186
Bpu10I CC'TnA_GC
Cuts at: 2205 2205
Size: 5923
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4048 4913 5211 4048
Size: 865 298 4760
Fragments arranged by size:
4760 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2645 4586 882
Size: 617 1146 1941 2219
Fragments arranged by size:
2219 1941 1146 617
BsaAI yAC'GTr
Cuts at: 493 1204 3714 493
Size: 711 2510 2702
Fragments arranged by size:
2702 2510 711
BsaBI GATnn'nnATC
Cuts at: 5917 5917
Size: 5923
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2460 2535 2739 2746
Size: 53 83 186 936 927 75 204 7
Cuts at: 2746 2969 3730 4112 4747 275
Size: 223 761 382 635 1451
Fragments arranged by size:
1451 936 927 761 635 382 223 204
186 83 75 53 7
BsaWI w'CCGG_w
Cuts at: 2172 2431 4356 4754 5028 5175 5586 2172
Size: 259 1925 398 274 147 411 2509
Fragments arranged by size:
2509 1925 411 398 274 259 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2684 2714 1178
Size: 30 165 30 1281 30 4387
Fragments arranged by size:
4387 1281 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5923
BseYI C'CCAG_C
Cuts at: 3068 3080 3128 5126 5622 3068
Size: 12 48 1998 496 3369
Fragments arranged by size:
3369 1998 496 48 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3083 1163
Size: 573 1347 4003
Fragments arranged by size:
4003 1347 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2783 3114 3216 3373 4134 5162
Size: 275 186 782 331 102 157 761 1028
Cuts at: 5162 1540
Size: 2301
Fragments arranged by size:
2301 1028 782 761 331 275 186 157
102
BsiHKAI G_wGCw'C
Cuts at: 2814 2991 3984 4069 5140 2814
Size: 177 993 85 1071 3597
Fragments arranged by size:
3597 1071 993 177 85
BsmI GAATG_Cn'
Cuts at: 3434 3527 3434
Size: 93 5830
BsmBI CGTCTCn'nnnn_
Cuts at: 2576 2576
Size: 5923
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2814 2871 2991 3081 3242 3984 4069
Size: 399 1024 57 120 90 161 742 85
Cuts at: 4069 5140 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 1024 742 399 161 120 90
85 57
BspEI T'CCGG_A
Cuts at: 2431 5586 2431
Size: 3155 2768
BspHI T'CATG_A
Cuts at: 3812 5542 3812
Size: 1730 4193
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2282 3278 844
Size: 1438 996 3489
Fragments arranged by size:
3489 1438 996
BsrBI CCG'CTC
Cuts at: 1394 1823 2710 3810 1394
Size: 429 887 1100 3507
Fragments arranged by size:
3507 1100 887 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4418 4592 65
Size: 1183 3170 174 1396
Fragments arranged by size:
3170 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2018 2654 2694 4567 4754 1700
Size: 110 208 636 40 1873 187 2869
Fragments arranged by size:
2869 1873 636 208 187 110 40
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5923
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5923
BssSI C'ACGA_G
Cuts at: 3983 4995 3983
Size: 1012 4911
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2995 794
Size: 2201 3722
BstBI TT'CG_AA
Cuts at: 4727 4727
Size: 5923
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5923
BstXI CCAn_nnnn'nTGG
Cuts at: 2578 2578
Size: 5923
BstYI r'GATC_y
Cuts at: 1972 3253 3620 4005 4022 5474 5560 5572 5918
Size: 1281 367 385 17 1452 86 12 346
Cuts at: 5918 1972
Size: 1977
Fragments arranged by size:
1977 1452 1281 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2406 513
Size: 552 939 402 4030
Fragments arranged by size:
4030 939 552 402
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5184
Fragments arranged by size:
5184 683 56
BtsI GCAGTG_nn'
Cuts at: 2377 2419 3439 4233 4253 2377
Size: 42 1020 794 20 4047
Fragments arranged by size:
4047 1020 794 42 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5923
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5888
DraI TTT'AAA
Cuts at: 3227 3227
Size: 5923
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4930 809
Size: 507 3614 1802
Fragments arranged by size:
3614 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2400 2780 3111
Size: 54 1425 50 275 18 570 380 331
Cuts at: 3111 3213 3370 4259 5888 8
Size: 102 157 889 1629 43
Fragments arranged by size:
1629 1425 889 570 380 331 275 157
102 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2780 3111 3213 3370 1537
Size: 275 968 331 102 157 4090
Fragments arranged by size:
4090 968 331 275 157 102
EarI CTCTTCn'nnn_
Cuts at: 1353 3853 1353
Size: 2500 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4510 4884 5030 387
Size: 1064 138 2921 374 146 1280
Fragments arranged by size:
2921 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2812 2812
Size: 5923
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2119 2600 2609 2819 3005 3986 4583
Size: 393 487 481 9 210 186 981 597
Cuts at: 4583 5370 5638 1239
Size: 787 268 1524
Fragments arranged by size:
1524 981 787 597 487 481 393 268
210 186 9
EcoO109I rG'GnC_Cy
Cuts at: 2070 2900 2070
Size: 830 5093
EcoRI G'AATT_C
Cuts at: 3232 3610 3232
Size: 378 5545
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2182 2614 2182
Size: 432 5491
FspI TGC'GCA
Cuts at: 2194 4429 2194
Size: 2235 3688
HaeII r_GCGC'y
Cuts at: 1678 2481 2538 2701 2733 2742 2749 5070 1678
Size: 803 57 163 32 9 7 2321 2531
Fragments arranged by size:
2531 2321 803 163 57 32 9 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4564 4596 4638 4670 1373
Size: 32 135 32 2992 32 42 32 2626
Fragments arranged by size:
2992 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3157 3267 3513 669
Size: 988 281 1219 110 246 3079
Fragments arranged by size:
3079 1219 988 281 246 110
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5923
HpaI GTT'AAC
Cuts at: 3513 3513
Size: 5923
KasI G'GCGC_C
Cuts at: 2534 2738 2745 2534
Size: 204 7 5712
Fragments arranged by size:
5712 204 7
KpnI G_GTAC'C
Cuts at: 2187 3248 2187
Size: 1061 4862
MfeI C'AATT_G
Cuts at: 3522 3522
Size: 5923
MluI A'CGCG_T
Cuts at: 5799 5799
Size: 5923
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2469 5037 5221 1305
Size: 1164 2568 184 2007
Fragments arranged by size:
2568 2007 1164 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4101
Fragments arranged by size:
4101 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2276 3881 4240 4399 518
Size: 648 1110 1605 359 159 2042
Fragments arranged by size:
2042 1605 1110 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2578 2641 2725 2785 2995 3028 3339 4016
Size: 972 63 84 60 210 33 311 677
Cuts at: 4016 5164 5409 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 972 677 311 245 210 84
63 60 33
NaeI GCC'GGC
Cuts at: 2020 2020
Size: 5923
NarI GG'CG_CC
Cuts at: 2535 2739 2746 2535
Size: 204 7 5712
Fragments arranged by size:
5712 204 7
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4432
Fragments arranged by size:
4432 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5923
NgoMIV G'CCGG_C
Cuts at: 2018 2018
Size: 5923
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5923
NotI GC'GGCC_GC
Cuts at: 2780 3370 2780
Size: 590 5333
NspI r_CATG'y
Cuts at: 1132 1229 2668 3281 4826 1132
Size: 97 1439 613 1545 2229
Fragments arranged by size:
2229 1545 1439 613 97
PasI CC'CwG_GG
Cuts at: 1348 2734 2974 1348
Size: 1386 240 4297
Fragments arranged by size:
4297 1386 240
PciI A'CATG_T
Cuts at: 4822 4822
Size: 5923
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2530 1108
Size: 1422 4501
PfoI T'CCnGG_A
Cuts at: 2037 2963 2037
Size: 926 4997
PmeI GTTT'AAAC
Cuts at: 3227 3227
Size: 5923
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2321 2353 2684 2716 3992 4024 5505 5537 2321
Size: 32 331 32 1276 32 1481 32 2707
Fragments arranged by size:
2707 1481 1276 331 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5923
PsiI TTA'TAA
Cuts at: 3493 3493
Size: 5923
PspOMI G'GGCC_C
Cuts at: 2867 2867
Size: 5923
PstI C_TGCA'G
Cuts at: 830 3275 830
Size: 2445 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5923
PvuII CAG'CTG
Cuts at: 2578 2641 2725 2578
Size: 63 84 5776
Fragments arranged by size:
5776 84 63
SacI G_AGCT'C
Cuts at: 2814 2814
Size: 5923
SalI G'TCGA_C
Cuts at: 1936 3265 1936
Size: 1329 4594
SbfI CC_TGCA'GG
Cuts at: 3275 3275
Size: 5923
ScaI AGT'ACT
Cuts at: 2861 4171 5883 2861
Size: 1310 1712 2901
Fragments arranged by size:
2901 1712 1310
SexAI A'CCwGG_T
Cuts at: 2226 2226
Size: 5923
SfcI C'TryA_G
Cuts at: 826 1045 3271 4406 5087 5278 826
Size: 219 2226 1135 681 191 1471
Fragments arranged by size:
2226 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2536 2740 2747 2536
Size: 204 7 5712
Fragments arranged by size:
5712 204 7
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5923
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5923
SmaI CCC'GGG
Cuts at: 1872 2872 3058 3091 3250 1872
Size: 1000 186 33 159 4545
Fragments arranged by size:
4545 1000 186 159 33
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4027 4928 5190 820
Size: 197 926 2084 901 262 1553
Fragments arranged by size:
2084 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3714 493
Size: 3221 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5923
SphI G_CATG'C
Cuts at: 1132 3281 1132
Size: 2149 3774
SrfI GCCC'GGGC
Cuts at: 3091 3091
Size: 5923
SspI AAT'ATT
Cuts at: 5 52 3847 5
Size: 47 3795 2081
Fragments arranged by size:
3795 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5923
StyI C'CwwG_G
Cuts at: 513 1065 2004 2463 2703 3362 513
Size: 552 939 459 240 659 3074
Fragments arranged by size:
3074 939 659 552 459 240
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2135 2626 2966 3066 3142 3950 3967
Size: 1255 555 491 340 100 76 808 17
Cuts at: 3967 4120 4305 4750 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 808 555 491 445 340 185
153 100 76 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2859 4169 5881
Size: 274 80 33 51 1443 882 1310 1712
Cuts at: 5881 96
Size: 138
Fragments arranged by size:
1712 1443 1310 882 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5883 5915 5883
Size: 32 5891
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5923
XbaI T'CTAG_A
Cuts at: 3259 3259
Size: 5923
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5923
XmaI C'CCGG_G
Cuts at: 1870 2870 3056 3089 3248 1870
Size: 1000 186 33 159 4545
Fragments arranged by size:
4545 1000 186 159 33
XmnI GAAnn'nnTTC
Cuts at: 2129 2165 2267 2336 4052 2129
Size: 36 102 69 1716 4000
Fragments arranged by size:
4000 1716 102 69 36
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3731 276
Size: 53 83 186 936 2197 2468
Fragments arranged by size:
2468 2197 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI
BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI NruI
NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII
SanDI SapI SfiI SwaI XcmI