Restriction map for
pFN21AB8140
(Circular) (Six-base) MAPSORT of: pFN21AB8140.seq Check: 1312 from: 1 to: 5518
pFN21AB8140 5518 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2288 3328 278
Size: 53 83 186 936 752 1040 2468
Fragments arranged by size:
2468 1040 936 752 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2310 2861 1937
Size: 373 551 4594
Fragments arranged by size:
4594 551 373
Acc65I G'GTAC_C
Cuts at: 2839 2839
Size: 5518
AclI AA'CG_TT
Cuts at: 1563 2237 3645 4018 1563
Size: 674 1408 373 3063
Fragments arranged by size:
3063 1408 674 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2366 2391 2531 3581 4965 5233 1632
Size: 734 25 140 1050 1384 268 1917
Fragments arranged by size:
1917 1384 1050 734 268 140 25
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5321
AflIII A'CryG_T
Cuts at: 1203 4417 5394 1203
Size: 3214 977 1327
Fragments arranged by size:
3214 1327 977
AgeI A'CCGG_T
Cuts at: 4349 4349
Size: 5518
AhdI GACnn_n'nnGTC
Cuts at: 4247 4247
Size: 5518
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5518
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2571 1184
Size: 1387 4131
AlwNI CAG_nnn'CTG
Cuts at: 2092 2593 3261 4833 2092
Size: 501 668 1572 2777
Fragments arranged by size:
2777 1572 668 501
ApaLI G'TGCA_C
Cuts at: 3575 4731 3575
Size: 1156 4362
ApoI r'AATT_y
Cuts at: 1493 2702 2827 3053 3205 5314 1493
Size: 1209 125 226 152 2109 1697
Fragments arranged by size:
2109 1697 1209 226 152 125
AseI AT'TA_AT
Cuts at: 160 1032 4072 160
Size: 872 3040 1606
Fragments arranged by size:
3040 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5518
AvaI C'yCGr_G
Cuts at: 1870 1943 2843 1870
Size: 73 900 4545
Fragments arranged by size:
4545 900 73
BamHI G'GATC_C
Cuts at: 2848 3215 2848
Size: 367 5151
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2045 2054 2839 4294 618
Size: 325 217 627 258 9 785 1455 1842
Fragments arranged by size:
1842 1455 785 627 325 258 217 9
BanII G_rGCy'C
Cuts at: 1391 2774 2837 1391
Size: 1383 63 4072
Fragments arranged by size:
4072 1383 63
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2152 2397 928
Size: 971 253 245 4049
Fragments arranged by size:
4049 971 253 245
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3709 3743 1903
Size: 34 1772 34 3678
Fragments arranged by size:
3678 1772 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3410 4620 3410
Size: 1210 4308
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5518
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4129 136
Size: 107 122 71 631 605 2457 1525
Fragments arranged by size:
2457 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5513 5513
Size: 5518
BlpI GC'TnA_GC
Cuts at: 2673 2935 2673
Size: 262 5256
Bme1580I G_kGCm'C
Cuts at: 1790 2057 3579 4735 1790
Size: 267 1522 1156 2573
Fragments arranged by size:
2573 1522 1156 267
BmgBI CAC'GTC
Cuts at: 1318 2767 1318
Size: 1449 4069
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3249 4207 442
Size: 416 53 83 399 24 1832 958 1753
Fragments arranged by size:
1832 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5518
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2615 2756 4178 1239
Size: 1376 141 1422 2579
Fragments arranged by size:
2579 1422 1376 141
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3643 4508 4806 3643
Size: 865 298 4355
Fragments arranged by size:
4355 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4181 882
Size: 617 2682 2219
Fragments arranged by size:
2682 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3309 493
Size: 711 2105 2702
Fragments arranged by size:
2702 2105 711
BsaBI GATnn'nnATC
Cuts at: 5512 5512
Size: 5518
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2285 3325 3707 4342
Size: 53 83 186 936 752 1040 382 635
Cuts at: 4342 275
Size: 1451
Fragments arranged by size:
1451 1040 936 752 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2175 3951 4349 4623 4770 5181 2175
Size: 1776 398 274 147 411 2512
Fragments arranged by size:
2512 1776 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5293
Fragments arranged by size:
5293 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5518
BseYI C'CCAG_C
Cuts at: 2639 2677 4721 5217 2639
Size: 38 2044 496 2940
Fragments arranged by size:
2940 2044 496 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2460 2761 1163
Size: 573 724 301 3920
Fragments arranged by size:
3920 724 573 301
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2968 3729 4757 1540
Size: 275 186 967 761 1028 2301
Fragments arranged by size:
2301 1028 967 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3579 3664 4735 3579
Size: 85 1071 4362
Fragments arranged by size:
4362 1071 85
BsmI GAATG_Cn'
Cuts at: 2444 3029 3122 2444
Size: 585 93 4840
Fragments arranged by size:
4840 585 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2057 2774 2837 3579 3664 4735 1391
Size: 399 267 717 63 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 717 399 267 85 63
BspEI T'CCGG_A
Cuts at: 2175 5181 2175
Size: 3006 2512
BspHI T'CATG_A
Cuts at: 3407 5137 3407
Size: 1730 3788
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2873 844
Size: 2029 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2395 3405 1394
Size: 429 572 1010 3507
Fragments arranged by size:
3507 1010 572 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2565 4013 4187 65
Size: 1183 1317 1448 174 1396
Fragments arranged by size:
1448 1396 1317 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4162 4349 1700
Size: 110 2352 187 2869
Fragments arranged by size:
2869 2352 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5518
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5518
BssSI C'ACGA_G
Cuts at: 3578 4590 3578
Size: 1012 4506
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2092 794
Size: 1298 4220
BstBI TT'CG_AA
Cuts at: 4322 4322
Size: 5518
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5518
BstXI CCAn_nnnn'nTGG
Cuts at: 2647 2647
Size: 5518
BstYI r'GATC_y
Cuts at: 1972 2848 3215 3600 3617 5069 5155 5167 5513
Size: 876 367 385 17 1452 86 12 346
Cuts at: 5513 1972
Size: 1977
Fragments arranged by size:
1977 1452 876 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2311 2311
Size: 5518
BtgI C'CryG_G
Cuts at: 513 1065 2004 2013 513
Size: 552 939 9 4018
Fragments arranged by size:
4018 939 552 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4779
Fragments arranged by size:
4779 683 56
BtsI GCAGTG_nn'
Cuts at: 2661 2749 3034 3828 3848 2661
Size: 88 285 794 20 4331
Fragments arranged by size:
4331 794 285 88 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5518
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5483
DraI TTT'AAA
Cuts at: 2822 2822
Size: 5518
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4525 809
Size: 507 3209 1802
Fragments arranged by size:
3209 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2362 2965 3854
Size: 54 1425 50 275 18 532 603 889
Cuts at: 3854 5483 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 603 532 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2965 1537
Size: 275 1153 4090
Fragments arranged by size:
4090 1153 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2752 3448 1353
Size: 1399 696 3423
Fragments arranged by size:
3423 1399 696
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4105 4479 4625 387
Size: 1064 138 2516 374 146 1280
Fragments arranged by size:
2516 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2366 2391 2531 2615 2756 3581 4178
Size: 393 734 25 140 84 141 825 597
Cuts at: 4178 4965 5233 1239
Size: 787 268 1524
Fragments arranged by size:
1524 825 787 734 597 393 268 141
140 84 25
EcoRI G'AATT_C
Cuts at: 2702 2827 3205 2702
Size: 125 378 5015
Fragments arranged by size:
5015 378 125
FspI TGC'GCA
Cuts at: 4024 4024
Size: 5518
HaeII r_GCGC'y
Cuts at: 1678 4665 1678
Size: 2987 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2756 2788 4159 4191 4233
Size: 32 135 32 1184 32 1371 32 42
Cuts at: 4233 4265 1373
Size: 32 2626
Fragments arranged by size:
2626 1371 1184 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2259 2320 2862 3108 669
Size: 988 281 321 61 542 246 3079
Fragments arranged by size:
3079 988 542 321 281 246 61
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5518
HpaI GTT'AAC
Cuts at: 2259 3108 2259
Size: 849 4669
KpnI G_GTAC'C
Cuts at: 2843 2843
Size: 5518
MfeI C'AATT_G
Cuts at: 3117 3117
Size: 5518
MluI A'CGCG_T
Cuts at: 5394 5394
Size: 5518
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4632 4816 1305
Size: 3327 184 2007
Fragments arranged by size:
3327 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3696
Fragments arranged by size:
3696 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3476 3835 3994 518
Size: 648 2310 359 159 2042
Fragments arranged by size:
2310 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2015 2301 2593 2677 2934 3611 4759 5004
Size: 409 286 292 84 257 677 1148 245
Cuts at: 5004 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 409 292 286 257 245
84
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4027
Fragments arranged by size:
4027 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5518
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5518
NotI GC'GGCC_GC
Cuts at: 2965 2965
Size: 5518
NspI r_CATG'y
Cuts at: 1132 1229 2876 4421 1132
Size: 97 1647 1545 2229
Fragments arranged by size:
2229 1647 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5518
PciI A'CATG_T
Cuts at: 4417 4417
Size: 5518
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2791 1108
Size: 1683 3835
PfoI T'CCnGG_A
Cuts at: 2228 2733 2784 2228
Size: 505 51 4962
Fragments arranged by size:
4962 505 51
PmeI GTTT'AAAC
Cuts at: 2822 2822
Size: 5518
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3587 3619 5100 5132 3587
Size: 32 1481 32 3973
Fragments arranged by size:
3973 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2156 1613
Size: 543 4975
PsiI TTA'TAA
Cuts at: 3088 3088
Size: 5518
PstI C_TGCA'G
Cuts at: 830 2097 2870 830
Size: 1267 773 3478
Fragments arranged by size:
3478 1267 773
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5518
PvuII CAG'CTG
Cuts at: 2301 2593 2677 2301
Size: 292 84 5142
Fragments arranged by size:
5142 292 84
SacII CC_GC'GG
Cuts at: 2016 2016
Size: 5518
SalI G'TCGA_C
Cuts at: 1936 2860 1936
Size: 924 4594
SbfI CC_TGCA'GG
Cuts at: 2870 2870
Size: 5518
ScaI AGT'ACT
Cuts at: 3766 5478 3766
Size: 1712 3806
SfcI C'TryA_G
Cuts at: 826 1045 2093 2866 4001 4682 4873 826
Size: 219 1048 773 1135 681 191 1471
Fragments arranged by size:
1471 1135 1048 773 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5518
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5518
SmaI CCC'GGG
Cuts at: 1872 2845 1872
Size: 973 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3622 4523 4785 820
Size: 197 926 1679 901 262 1553
Fragments arranged by size:
1679 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3309 493
Size: 2816 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5518
SphI G_CATG'C
Cuts at: 1132 2876 1132
Size: 1744 3774
SspI AAT'ATT
Cuts at: 5 52 3442 5
Size: 47 3390 2081
Fragments arranged by size:
3390 2081 47
StuI AGG'CCT
Cuts at: 1517 2265 1517
Size: 748 4770
StyI C'CwwG_G
Cuts at: 513 1065 2004 2748 2957 513
Size: 552 939 744 209 3074
Fragments arranged by size:
3074 939 744 552 209
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3545 3562 3715 3900 4345 325
Size: 1255 1965 17 153 185 445 1498
Fragments arranged by size:
1965 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3764 5476 96
Size: 274 80 33 51 1443 1787 1712 138
Fragments arranged by size:
1787 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5478 5510 5478
Size: 32 5486
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5518
XbaI T'CTAG_A
Cuts at: 2854 2854
Size: 5518
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5518
XmaI C'CCGG_G
Cuts at: 1870 2843 1870
Size: 973 4545
XmnI GAAnn'nnTTC
Cuts at: 3647 3647
Size: 5518
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2286 3326 276
Size: 53 83 186 936 752 1040 2468
Fragments arranged by size:
2468 1040 936 752 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SacII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI