Restriction map for
pFN21AB8139
(Circular) (Six-base) MAPSORT of: pFN21AB8139.seq Check: 4567 from: 1 to: 5683
pFN21AB8139 5683 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2291 2291
Size: 5683
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3493 278
Size: 53 83 186 936 1957 2468
Fragments arranged by size:
2468 1957 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3026 1937
Size: 1089 4594
Acc65I G'GTAC_C
Cuts at: 3004 3004
Size: 5683
AclI AA'CG_TT
Cuts at: 1563 3810 4183 1563
Size: 2247 373 3063
Fragments arranged by size:
3063 2247 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3746 5130 5398 1632
Size: 2114 1384 268 1917
Fragments arranged by size:
2114 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5486
AflIII A'CryG_T
Cuts at: 1203 2454 4582 5559 1203
Size: 1251 2128 977 1327
Fragments arranged by size:
2128 1327 1251 977
AgeI A'CCGG_T
Cuts at: 4514 4514
Size: 5683
AhdI GACnn_n'nnGTC
Cuts at: 4412 4412
Size: 5683
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5683
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5683
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2364 2396 2364
Size: 32 5651
AlwNI CAG_nnn'CTG
Cuts at: 2128 3426 4998 2128
Size: 1298 1572 2813
Fragments arranged by size:
2813 1572 1298
ApaI G_GGCC'C
Cuts at: 2121 2121
Size: 5683
ApaLI G'TGCA_C
Cuts at: 3740 4896 3740
Size: 1156 4527
ApoI r'AATT_y
Cuts at: 1493 2992 3218 3370 5479 1493
Size: 1499 226 152 2109 1697
Fragments arranged by size:
2109 1697 1499 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4237 160
Size: 872 3205 1606
Fragments arranged by size:
3205 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5683
AvaI C'yCGr_G
Cuts at: 1870 1943 2066 3008 1870
Size: 73 123 942 4545
Fragments arranged by size:
4545 942 123 73
BamHI G'GATC_C
Cuts at: 3013 3380 3013
Size: 367 5316
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2817 3004 4459 618
Size: 325 217 627 1030 187 1455 1842
Fragments arranged by size:
1842 1455 1030 627 325 217 187
BanII G_rGCy'C
Cuts at: 1391 2121 3002 1391
Size: 730 881 4072
Fragments arranged by size:
4072 881 730
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2419 928
Size: 971 520 4192
Fragments arranged by size:
4192 971 520
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2047 2081 2779 2813 3874 3908 1903
Size: 34 110 34 698 34 1061 34 3678
Fragments arranged by size:
3678 1061 698 110 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2148 2784 3575 4785 2148
Size: 636 791 1210 3046
Fragments arranged by size:
3046 1210 791 636
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5683
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2125 2166 2565
Size: 107 122 71 631 605 453 41 399
Cuts at: 2565 2687 4294 136
Size: 122 1607 1525
Fragments arranged by size:
1607 1525 631 605 453 399 122 122
107 71 41
BglII A'GATC_T
Cuts at: 5678 5678
Size: 5683
BlpI GC'TnA_GC
Cuts at: 3100 3100
Size: 5683
Bme1580I G_kGCm'C
Cuts at: 1790 2121 2759 3744 4900 1790
Size: 331 638 985 1156 2573
Fragments arranged by size:
2573 1156 985 638 331
BmgBI CAC'GTC
Cuts at: 1318 2455 1318
Size: 1137 4546
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3414 4372 442
Size: 416 53 83 399 24 1997 958 1753
Fragments arranged by size:
1997 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5683
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2092 2150 2839 4343 1239
Size: 853 58 689 1504 2579
Fragments arranged by size:
2579 1504 853 689 58
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3808 4673 4971 3808
Size: 865 298 4520
Fragments arranged by size:
4520 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2219 4346 882
Size: 617 720 2127 2219
Fragments arranged by size:
2219 2127 720 617
BsaAI yAC'GTr
Cuts at: 493 1204 2044 2273 3474 493
Size: 711 840 229 1201 2702
Fragments arranged by size:
2702 1201 840 711 229
BsaBI GATnn'nnATC
Cuts at: 5677 5677
Size: 5683
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3490 3872 4507 275
Size: 53 83 186 936 1957 382 635 1451
Fragments arranged by size:
1957 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2076 2097 4116 4514 4788 4935 5346 2076
Size: 21 2019 398 274 147 411 2413
Fragments arranged by size:
2413 2019 411 398 274 147 21
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2364 2394 1178
Size: 30 165 30 961 30 4467
Fragments arranged by size:
4467 961 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2321 2900 1186
Size: 1135 579 3969
Fragments arranged by size:
3969 1135 579
BseYI C'CCAG_C
Cuts at: 2121 2615 2942 4886 5382 2121
Size: 494 327 1944 496 2422
Fragments arranged by size:
2422 1944 496 494 327
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2417 2764 1163
Size: 573 681 347 4082
Fragments arranged by size:
4082 681 573 347
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3133 3894 4922 1540
Size: 275 186 1132 761 1028 2301
Fragments arranged by size:
2301 1132 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3744 3829 4900 3744
Size: 85 1071 4527
Fragments arranged by size:
4527 1071 85
BsmI GAATG_Cn'
Cuts at: 3194 3287 3194
Size: 93 5590
BsmBI CGTCTCn'nnnn_
Cuts at: 2539 2539
Size: 5683
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2121 2759 3002 3744 3829 4900 1391
Size: 399 331 638 243 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 638 399 331 243 85
BspEI T'CCGG_A
Cuts at: 2097 5346 2097
Size: 3249 2434
BspHI T'CATG_A
Cuts at: 3572 5302 3572
Size: 1730 3953
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2291 3038 844
Size: 1447 747 3489
Fragments arranged by size:
3489 1447 747
BsrBI CCG'CTC
Cuts at: 1394 1823 3570 1394
Size: 429 1747 3507
Fragments arranged by size:
3507 1747 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4178 4352 65
Size: 1183 2930 174 1396
Fragments arranged by size:
2930 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4327 4514 1700
Size: 110 2517 187 2869
Fragments arranged by size:
2869 2517 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5683
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5683
BssSI C'ACGA_G
Cuts at: 2486 2536 3743 4755 2486
Size: 50 1207 1012 3414
Fragments arranged by size:
3414 1207 1012 50
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5683
BstBI TT'CG_AA
Cuts at: 4487 4487
Size: 5683
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5683
BstXI CCAn_nnnn'nTGG
Cuts at: 2828 2828
Size: 5683
BstYI r'GATC_y
Cuts at: 1972 2070 3013 3380 3765 3782 5234 5320 5332
Size: 98 943 367 385 17 1452 86 12
Cuts at: 5332 5678 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 943 385 367 346 98 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4192
Fragments arranged by size:
4192 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2677 2827 487
Size: 683 56 1451 150 3343
Fragments arranged by size:
3343 1451 683 150 56
BtsI GCAGTG_nn'
Cuts at: 2405 2552 2812 2965 3199 3993 4013 2405
Size: 147 260 153 234 794 20 4075
Fragments arranged by size:
4075 794 260 234 153 147 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5683
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5648
DraI TTT'AAA
Cuts at: 2987 2987
Size: 5683
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4690 809
Size: 507 3374 1802
Fragments arranged by size:
3374 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2244 2566 2599
Size: 54 1425 50 275 18 414 322 33
Cuts at: 2599 2688 3130 4019 5648 8
Size: 89 442 889 1629 43
Fragments arranged by size:
1629 1425 889 442 414 322 275 89
54 50 43 33 18
EagI C'GGCC_G
Cuts at: 1537 1812 3130 1537
Size: 275 1318 4090
Fragments arranged by size:
4090 1318 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2048 3613 1353
Size: 695 1565 3423
Fragments arranged by size:
3423 1565 695
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4270 4644 4790 387
Size: 1064 138 2681 374 146 1280
Fragments arranged by size:
2681 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2092 2150 2839 3746 4343 5130 5398
Size: 393 460 58 689 907 597 787 268
Cuts at: 5398 1239
Size: 1524
Fragments arranged by size:
1524 907 787 689 597 460 393 268
58
EcoO109I rG'GnC_Cy
Cuts at: 2117 2118 2343 2713 2117
Size: 1 225 370 5087
Fragments arranged by size:
5087 370 225 1
EcoRI G'AATT_C
Cuts at: 2992 3370 2992
Size: 378 5305
FspI TGC'GCA
Cuts at: 4189 4189
Size: 5683
HaeII r_GCGC'y
Cuts at: 1678 4830 1678
Size: 3152 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2135 2167 4324 4356 4398
Size: 32 135 32 563 32 2157 32 42
Cuts at: 4398 4430 1373
Size: 32 2626
Fragments arranged by size:
2626 2157 563 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3027 3273 669
Size: 988 281 1089 246 3079
Fragments arranged by size:
3079 1089 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5683
HpaI GTT'AAC
Cuts at: 3273 3273
Size: 5683
KpnI G_GTAC'C
Cuts at: 3008 3008
Size: 5683
MfeI C'AATT_G
Cuts at: 3282 3282
Size: 5683
MluI A'CGCG_T
Cuts at: 5559 5559
Size: 5683
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4797 4981 1305
Size: 3492 184 2007
Fragments arranged by size:
3492 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2246 2690 10
Size: 54 1425 343 414 444 3003
Fragments arranged by size:
3003 1425 444 414 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2792 3641 4000 4159 518
Size: 648 1626 849 359 159 2042
Fragments arranged by size:
2042 1626 849 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2125 3099 3776 4924 5169 1606
Size: 519 974 677 1148 245 2120
Fragments arranged by size:
2120 1148 974 677 519 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4192
Fragments arranged by size:
4192 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5683
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5683
NotI GC'GGCC_GC
Cuts at: 3130 3130
Size: 5683
NspI r_CATG'y
Cuts at: 1132 1229 3041 4586 1132
Size: 97 1812 1545 2229
Fragments arranged by size:
2229 1812 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2390 1348
Size: 1042 4641
PciI A'CATG_T
Cuts at: 4582 4582
Size: 5683
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2128 1108
Size: 1020 4663
PmeI GTTT'AAAC
Cuts at: 2987 2987
Size: 5683
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2364 2396 3752 3784 5265 5297 2364
Size: 32 1356 32 1481 32 2750
Fragments arranged by size:
2750 1481 1356 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2343 2713 2343
Size: 370 5313
PshAI GACnn'nnGTC
Cuts at: 1613 2361 1613
Size: 748 4935
PsiI TTA'TAA
Cuts at: 3253 3253
Size: 5683
PspOMI G'GGCC_C
Cuts at: 2117 2117
Size: 5683
PstI C_TGCA'G
Cuts at: 830 2478 3035 830
Size: 1648 557 3478
Fragments arranged by size:
3478 1648 557
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5683
SalI G'TCGA_C
Cuts at: 1936 3025 1936
Size: 1089 4594
SanDI GG'GwC_CC
Cuts at: 2343 2343
Size: 5683
SapI GCTCTTCn'nnn_
Cuts at: 2048 2048
Size: 5683
SbfI CC_TGCA'GG
Cuts at: 3035 3035
Size: 5683
ScaI AGT'ACT
Cuts at: 3931 5643 3931
Size: 1712 3971
SfcI C'TryA_G
Cuts at: 826 1045 2474 3031 4166 4847 5038 826
Size: 219 1429 557 1135 681 191 1471
Fragments arranged by size:
1471 1429 1135 681 557 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5683
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5683
SmaI CCC'GGG
Cuts at: 1872 3010 1872
Size: 1138 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3787 4688 4950 820
Size: 197 926 1844 901 262 1553
Fragments arranged by size:
1844 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3474 493
Size: 2981 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5683
SphI G_CATG'C
Cuts at: 1132 3041 1132
Size: 1909 3774
SspI AAT'ATT
Cuts at: 5 52 3607 5
Size: 47 3555 2081
Fragments arranged by size:
3555 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5683
StyI C'CwwG_G
Cuts at: 513 1065 2004 2175 2220 3122 513
Size: 552 939 171 45 902 3074
Fragments arranged by size:
3074 939 902 552 171 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2498 2800 3710 3727 3880 4065 4510
Size: 1255 918 302 910 17 153 185 445
Cuts at: 4510 325
Size: 1498
Fragments arranged by size:
1498 1255 918 910 445 302 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3929 5641 96
Size: 274 80 33 51 1443 1952 1712 138
Fragments arranged by size:
1952 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5643 5675 5643
Size: 32 5651
Tth111I GACn'n_nGTC
Cuts at: 1316 2027 1316
Size: 711 4972
XbaI T'CTAG_A
Cuts at: 3019 3019
Size: 5683
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5683
XmaI C'CCGG_G
Cuts at: 1870 3008 1870
Size: 1138 4545
XmnI GAAnn'nnTTC
Cuts at: 3812 3812
Size: 5683
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3491 276
Size: 53 83 186 936 1957 2468
Fragments arranged by size:
2468 1957 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI SalI SanDI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII
SacI SacII SexAI SfiI SfoI SrfI SwaI XcmI