Restriction map for
pFN21AB8132
(Circular) (Six-base) MAPSORT of: pFN21AB8132.seq Check: 4251 from: 1 to: 6235
pFN21AB8132 6235 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4045 278
Size: 53 83 186 936 2509 2468
Fragments arranged by size:
2509 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2172 3578 1937
Size: 235 1406 4594
Fragments arranged by size:
4594 1406 235
Acc65I G'GTAC_C
Cuts at: 3556 3556
Size: 6235
AclI AA'CG_TT
Cuts at: 1563 4362 4735 1563
Size: 2799 373 3063
Fragments arranged by size:
3063 2799 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2672 2741 3343 4298 5682 5950 1632
Size: 1040 69 602 955 1384 268 1917
Fragments arranged by size:
1917 1384 1040 955 602 268 69
AfeI AGC'GCT
Cuts at: 2866 3323 2866
Size: 457 5778
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6038
AflIII A'CryG_T
Cuts at: 1203 5134 6111 1203
Size: 3931 977 1327
Fragments arranged by size:
3931 1327 977
AgeI A'CCGG_T
Cuts at: 5066 5066
Size: 6235
AhdI GACnn_n'nnGTC
Cuts at: 3028 4964 3028
Size: 1936 4299
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6235
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6235
AlwNI CAG_nnn'CTG
Cuts at: 2203 2236 2269 2296 2650 2680 3978 5550 2203
Size: 33 33 27 354 30 1298 1572 2888
Fragments arranged by size:
2888 1572 1298 354 33 33 30 27
ApaI G_GGCC'C
Cuts at: 2193 3203 3434 2193
Size: 1010 231 4994
Fragments arranged by size:
4994 1010 231
ApaLI G'TGCA_C
Cuts at: 4292 5448 4292
Size: 1156 5079
ApoI r'AATT_y
Cuts at: 1493 2165 3544 3770 3922 6031 1493
Size: 672 1379 226 152 2109 1697
Fragments arranged by size:
2109 1697 1379 672 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4789 160
Size: 872 3757 1606
Fragments arranged by size:
3757 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6235
AvaI C'yCGr_G
Cuts at: 1870 1943 2105 3125 3149 3426 3560 1870
Size: 73 162 1020 24 277 134 4545
Fragments arranged by size:
4545 1020 277 162 134 73 24
BamHI G'GATC_C
Cuts at: 3565 3932 3565
Size: 367 5868
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2542 2926 2963 3422 3556
Size: 325 217 627 755 384 37 459 134
Cuts at: 3556 5011 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 755 627 459 384 325 217
134 37
BanII G_rGCy'C
Cuts at: 1391 2045 2193 2423 2435 2502 2510 3203 3434
Size: 654 148 230 12 67 8 693 231
Cuts at: 3434 3554 1391
Size: 120 4072
Fragments arranged by size:
4072 693 654 231 230 148 120 67
12 8
BbeI G_GCGC'C
Cuts at: 2930 3426 2930
Size: 496 5739
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3353 3392 928
Size: 971 1454 39 3771
Fragments arranged by size:
3771 1454 971 39
BbvCI CC'TCA_GC
Cuts at: 2673 2673
Size: 6235
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4426 4460 1903
Size: 34 2489 34 3678
Fragments arranged by size:
3678 2489 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4127 5337 4127
Size: 1210 5025
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6235
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3153 3312 4846
Size: 107 122 71 631 605 1481 159 1534
Cuts at: 4846 136
Size: 1525
Fragments arranged by size:
1534 1525 1481 631 605 159 122 107
71
BglII A'GATC_T
Cuts at: 6230 6230
Size: 6235
BlpI GC'TnA_GC
Cuts at: 3652 3652
Size: 6235
Bme1580I G_kGCm'C
Cuts at: 1790 2193 2966 3095 3203 3434 4296 5452 1790
Size: 403 773 129 108 231 862 1156 2573
Fragments arranged by size:
2573 1156 862 773 403 231 129 108
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6235
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3113 3966 4924
Size: 416 53 83 399 24 1696 853 958
Cuts at: 4924 442
Size: 1753
Fragments arranged by size:
1753 1696 958 853 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6235
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2269 3052 3175 2269
Size: 783 123 5329
Fragments arranged by size:
5329 783 123
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2159 2291 2306 2764 3206 4895 1239
Size: 920 132 15 458 442 1689 2579
Fragments arranged by size:
2579 1689 920 458 442 132 15
Bpu10I CC'TnA_GC
Cuts at: 2673 2910 2673
Size: 237 5998
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4360 5225 5523 4360
Size: 865 298 5072
Fragments arranged by size:
5072 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2102 4898 882
Size: 617 603 2796 2219
Fragments arranged by size:
2796 2219 617 603
BsaAI yAC'GTr
Cuts at: 493 1204 4026 493
Size: 711 2822 2702
Fragments arranged by size:
2822 2702 711
BsaBI GATnn'nnATC
Cuts at: 6229 6229
Size: 6235
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2927 2940 3156 3423
Size: 53 83 186 936 1394 13 216 267
Cuts at: 3423 4042 4424 5059 275
Size: 619 382 635 1451
Fragments arranged by size:
1451 1394 936 635 619 382 267 216
186 83 53 13
BsaWI w'CCGG_w
Cuts at: 2569 2730 2802 3044 4668 5066 5340 5487 5898
Size: 161 72 242 1624 398 274 147 411
Cuts at: 5898 2569
Size: 2906
Fragments arranged by size:
2906 1624 411 398 274 242 161 147
72
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2009 2039 2762 2792 1178
Size: 30 165 30 606 30 723 30 4621
Fragments arranged by size:
4621 723 606 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2608 2726 2897 2987 2990 1186
Size: 1422 118 171 90 3 4431
Fragments arranged by size:
4431 1422 171 118 90 3
BseYI C'CCAG_C
Cuts at: 2047 2416 2510 2528 2763 2867 3415 5438 5934
Size: 369 94 18 235 104 548 2023 496
Cuts at: 5934 2047
Size: 2348
Fragments arranged by size:
2348 2023 548 496 369 235 104 94
18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5662
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3316 3685 4446 5474 1540
Size: 275 186 1315 369 761 1028 2301
Fragments arranged by size:
2301 1315 1028 761 369 275 186
BsiHKAI G_wGCw'C
Cuts at: 2045 2502 3297 3381 4296 4381 5452 2045
Size: 457 795 84 915 85 1071 2828
Fragments arranged by size:
2828 1071 915 795 457 85 84
BsmI GAATG_Cn'
Cuts at: 3746 3839 3746
Size: 93 6142
BsmBI CGTCTCn'nnnn_
Cuts at: 3163 3163
Size: 6235
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2045 2193 2423 2435 2502 2510 2966
Size: 399 255 148 230 12 67 8 456
Cuts at: 2966 3095 3203 3297 3381 3434 3554 4296 4381
Size: 129 108 94 84 53 120 742 85
Cuts at: 4381 5452 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 456 399 255 230 148
129 120 108 94 85 84 67 53
12 8
BspEI T'CCGG_A
Cuts at: 2569 5898 2569
Size: 3329 2906
BspHI T'CATG_A
Cuts at: 4124 5854 4124
Size: 1730 4505
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3590 844
Size: 2746 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4122 1394
Size: 429 2299 3507
Fragments arranged by size:
3507 2299 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4730 4904 65
Size: 1183 3482 174 1396
Fragments arranged by size:
3482 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4879 5066 1700
Size: 110 3069 187 2869
Fragments arranged by size:
3069 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6235
BssHII G'CGCG_C
Cuts at: 1925 2249 1925
Size: 324 5911
BssSI C'ACGA_G
Cuts at: 3392 4295 5307 3392
Size: 903 1012 4320
Fragments arranged by size:
4320 1012 903
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6235
BstBI TT'CG_AA
Cuts at: 5039 5039
Size: 6235
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6235
BstXI CCAn_nnnn'nTGG
Cuts at: 2522 2522
Size: 6235
BstYI r'GATC_y
Cuts at: 1972 2059 2535 2818 3565 3932 4317 4334 5786
Size: 87 476 283 747 367 385 17 1452
Cuts at: 5786 5872 5884 6230 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 747 476 385 367 346 283
87 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2244 2244
Size: 6235
BtgI C'CryG_G
Cuts at: 513 1065 2895 3229 3307 513
Size: 552 1830 334 78 3441
Fragments arranged by size:
3441 1830 552 334 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2466 487
Size: 683 56 1240 4256
Fragments arranged by size:
4256 1240 683 56
BtsI GCAGTG_nn'
Cuts at: 3751 4545 4565 3751
Size: 794 20 5421
Fragments arranged by size:
5421 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6235
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6200
DraI TTT'AAA
Cuts at: 3539 3539
Size: 6235
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5242 809
Size: 507 3926 1802
Fragments arranged by size:
3926 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3313 3682 4571
Size: 54 1425 50 275 18 1483 369 889
Cuts at: 4571 6200 8
Size: 1629 43
Fragments arranged by size:
1629 1483 1425 889 369 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3313 3682 1537
Size: 275 1501 369 4090
Fragments arranged by size:
4090 1501 369 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2394 2613 2717 2976 4165 1353
Size: 1041 219 104 259 1189 3423
Fragments arranged by size:
3423 1189 1041 259 219 104
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3492 4822 5196 5342 387
Size: 1064 138 1903 1330 374 146 1280
Fragments arranged by size:
1903 1330 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2043 2500 2043
Size: 457 5778
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2159 2291 2306 2672 2741 2764 3206
Size: 393 527 132 15 366 69 23 442
Cuts at: 3206 3343 4298 4895 5682 5950 1239
Size: 137 955 597 787 268 1524
Fragments arranged by size:
1524 955 787 597 527 442 393 366
268 137 132 69 23 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2035 2375 2035
Size: 340 5895
EcoO109I rG'GnC_Cy
Cuts at: 2124 2190 2380 2518 2668 2998 3013 3199 3200
Size: 66 190 138 150 330 15 186 1
Cuts at: 3200 3430 3431 2124
Size: 230 1 4928
Fragments arranged by size:
4928 330 230 190 186 150 138 66
15 1 1
EcoRI G'AATT_C
Cuts at: 3544 3922 3544
Size: 378 5857
FspI TGC'GCA
Cuts at: 4741 4741
Size: 6235
HaeII r_GCGC'y
Cuts at: 1678 2868 2930 3325 3426 5382 1678
Size: 1190 62 395 101 1956 2531
Fragments arranged by size:
2531 1956 1190 395 101 62
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2762 2794 2942 2974 3014
Size: 32 135 32 1190 32 148 32 40
Cuts at: 3014 3046 4876 4908 4950 4982 1373
Size: 32 1830 32 42 32 2626
Fragments arranged by size:
2626 1830 1190 148 135 42 40 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3579 3825 669
Size: 988 281 1641 246 3079
Fragments arranged by size:
3079 1641 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6235
HpaI GTT'AAC
Cuts at: 3825 3825
Size: 6235
KasI G'GCGC_C
Cuts at: 2926 3422 2926
Size: 496 5739
KpnI G_GTAC'C
Cuts at: 3560 3560
Size: 6235
MfeI C'AATT_G
Cuts at: 3834 3834
Size: 6235
MluI A'CGCG_T
Cuts at: 6111 6111
Size: 6235
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3057 5349 5533 1305
Size: 1752 2292 184 2007
Fragments arranged by size:
2292 2007 1752 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4413
Fragments arranged by size:
4413 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4193 4552 4711 518
Size: 648 3027 359 159 2042
Fragments arranged by size:
3027 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2897 3231 3651 4328 5476 5721 1606
Size: 1291 334 420 677 1148 245 2120
Fragments arranged by size:
2120 1291 1148 677 420 334 245
NarI GG'CG_CC
Cuts at: 2927 3423 2927
Size: 496 5739
NcoI C'CATG_G
Cuts at: 513 1065 3307 513
Size: 552 2242 3441
Fragments arranged by size:
3441 2242 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6235
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6235
NotI GC'GGCC_GC
Cuts at: 3682 3682
Size: 6235
NruI TCG'CGA
Cuts at: 2504 2504
Size: 6235
NspI r_CATG'y
Cuts at: 1132 1229 3593 5138 1132
Size: 97 2364 1545 2229
Fragments arranged by size:
2364 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2325 2933 3203 1348
Size: 977 608 270 4380
Fragments arranged by size:
4380 977 608 270
PciI A'CATG_T
Cuts at: 5134 5134
Size: 6235
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2203 1108
Size: 1095 5140
PfoI T'CCnGG_A
Cuts at: 2160 2394 2160
Size: 234 6001
PmeI GTTT'AAAC
Cuts at: 3539 3539
Size: 6235
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4304 4336 5817 5849 4304
Size: 32 1481 32 4690
Fragments arranged by size:
4690 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2124 2668 3013 2124
Size: 544 345 5346
Fragments arranged by size:
5346 544 345
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6235
PsiI TTA'TAA
Cuts at: 3805 3805
Size: 6235
PspOMI G'GGCC_C
Cuts at: 2189 3199 3430 2189
Size: 1010 231 4994
Fragments arranged by size:
4994 1010 231
PspXI vC'TCGA_Gb
Cuts at: 3149 3149
Size: 6235
PstI C_TGCA'G
Cuts at: 830 3587 830
Size: 2757 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6235
SacI G_AGCT'C
Cuts at: 2045 2502 2045
Size: 457 5778
SacII CC_GC'GG
Cuts at: 2898 3232 2898
Size: 334 5901
SalI G'TCGA_C
Cuts at: 1936 3577 1936
Size: 1641 4594
SapI GCTCTTCn'nnn_
Cuts at: 2394 2717 2976 2394
Size: 323 259 5653
Fragments arranged by size:
5653 323 259
SbfI CC_TGCA'GG
Cuts at: 3587 3587
Size: 6235
ScaI AGT'ACT
Cuts at: 4483 6195 4483
Size: 1712 4523
SfcI C'TryA_G
Cuts at: 826 1045 3583 4718 5399 5590 826
Size: 219 2538 1135 681 191 1471
Fragments arranged by size:
2538 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2928 3424 2928
Size: 496 5739
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6235
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6235
SmaI CCC'GGG
Cuts at: 1872 3428 3562 1872
Size: 1556 134 4545
Fragments arranged by size:
4545 1556 134
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3149 4339 5240 5502 820
Size: 197 926 1206 1190 901 262 1553
Fragments arranged by size:
1553 1206 1190 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4026 493
Size: 3533 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6235
SphI G_CATG'C
Cuts at: 1132 3593 1132
Size: 2461 3774
SspI AAT'ATT
Cuts at: 5 52 4159 5
Size: 47 4107 2081
Fragments arranged by size:
4107 2081 47
StuI AGG'CCT
Cuts at: 1517 3266 1517
Size: 1749 4486
StyI C'CwwG_G
Cuts at: 513 1065 2256 2521 3307 3674 513
Size: 552 1191 265 786 367 3074
Fragments arranged by size:
3074 1191 786 552 367 265
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2317 2630 2859 4262 4279 4432 4617
Size: 1255 737 313 229 1403 17 153 185
Cuts at: 4617 5062 325
Size: 445 1498
Fragments arranged by size:
1498 1403 1255 737 445 313 229 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4481 6193 96
Size: 274 80 33 51 1443 2504 1712 138
Fragments arranged by size:
2504 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6195 6227 6195
Size: 32 6203
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6235
XbaI T'CTAG_A
Cuts at: 3571 3571
Size: 6235
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 3305 2011
Size: 1294 4941
XhoI C'TCGA_G
Cuts at: 1943 3149 1943
Size: 1206 5029
XmaI C'CCGG_G
Cuts at: 1870 3426 3560 1870
Size: 1556 134 4545
Fragments arranged by size:
4545 1556 134
XmnI GAAnn'nnTTC
Cuts at: 3350 4364 3350
Size: 1014 5221
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4043 276
Size: 53 83 186 936 2509 2468
Fragments arranged by size:
2509 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NruI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I
DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NsiI
PacI PmlI PsrI PvuII RsrII SanDI SexAI SfiI
SrfI SwaI