Restriction map for
pFN21AB8130
(Circular) (Six-base) MAPSORT of: pFN21AB8130.seq Check: 4097 from: 1 to: 6046
pFN21AB8130 6046 bp
With 182 enzymes: SgfI *
August 16, 2010 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2440 2440
Size: 6046
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2444 3856 278
Size: 53 83 186 936 908 1412 2468
Fragments arranged by size:
2468 1412 936 908 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2721 3389 1937
Size: 784 668 4594
Fragments arranged by size:
4594 784 668
Acc65I G'GTAC_C
Cuts at: 3367 3367
Size: 6046
AclI AA'CG_TT
Cuts at: 1563 4173 4546 1563
Size: 2610 373 3063
Fragments arranged by size:
3063 2610 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2420 2483 4109 5493 5761 1632
Size: 788 63 1626 1384 268 1917
Fragments arranged by size:
1917 1626 1384 788 268 63
AflII C'TTAA_G
Cuts at: 820 1017 2420 820
Size: 197 1403 4446
Fragments arranged by size:
4446 1403 197
AflIII A'CryG_T
Cuts at: 1203 2208 2568 4945 5922 1203
Size: 1005 360 2377 977 1327
Fragments arranged by size:
2377 1327 1005 977 360
AgeI A'CCGG_T
Cuts at: 4877 4877
Size: 6046
AhdI GACnn_n'nnGTC
Cuts at: 4775 4775
Size: 6046
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6046
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6046
AlwNI CAG_nnn'CTG
Cuts at: 2556 2687 3789 5361 2556
Size: 131 1102 1572 3241
Fragments arranged by size:
3241 1572 1102 131
ApaLI G'TGCA_C
Cuts at: 4103 5259 4103
Size: 1156 4890
ApoI r'AATT_y
Cuts at: 1493 3355 3581 3733 5842 1493
Size: 1862 226 152 2109 1697
Fragments arranged by size:
2109 1862 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4600 160
Size: 872 3568 1606
Fragments arranged by size:
3568 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6046
AvaI C'yCGr_G
Cuts at: 1870 1943 2898 3136 3156 3371 1870
Size: 73 955 238 20 215 4545
Fragments arranged by size:
4545 955 238 215 73 20
BamHI G'GATC_C
Cuts at: 3376 3743 3376
Size: 367 5679
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2452 2601 3264 3367 4822
Size: 325 217 627 665 149 663 103 1455
Cuts at: 4822 618
Size: 1842
Fragments arranged by size:
1842 1455 665 663 627 325 217 149
103
BanII G_rGCy'C
Cuts at: 1391 2495 2741 3365 1391
Size: 1104 246 624 4072
Fragments arranged by size:
4072 1104 624 246
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2072 928
Size: 971 173 4902
Fragments arranged by size:
4902 971 173
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2698 2732 4237 4271 1903
Size: 34 761 34 1505 34 3678
Fragments arranged by size:
3678 1505 761 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2384 3938 5148 2384
Size: 1554 1210 3282
Fragments arranged by size:
3282 1554 1210
BclI T'GATC_A
Cuts at: 1548 2676 1548
Size: 1128 4918
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2880 4657 136
Size: 107 122 71 631 605 1208 1777 1525
Fragments arranged by size:
1777 1525 1208 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6041 6041
Size: 6046
BlpI GC'TnA_GC
Cuts at: 3463 3463
Size: 6046
Bme1580I G_kGCm'C
Cuts at: 1790 4107 5263 1790
Size: 2317 1156 2573
Fragments arranged by size:
2573 2317 1156
BmgBI CAC'GTC
Cuts at: 1318 2209 2863 3121 1318
Size: 891 654 258 4243
Fragments arranged by size:
4243 891 654 258
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2811 3777 4735
Size: 416 53 83 399 24 1394 966 958
Cuts at: 4735 442
Size: 1753
Fragments arranged by size:
1753 1394 966 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6046
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2055 2282 3149 4706 1239
Size: 816 227 867 1557 2579
Fragments arranged by size:
2579 1557 867 816 227
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2237 3152 4171 5036 5334 2237
Size: 915 1019 865 298 2949
Fragments arranged by size:
2949 1019 915 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2555 2948 4709 882
Size: 617 1056 393 1761 2219
Fragments arranged by size:
2219 1761 1056 617 393
BsaAI yAC'GTr
Cuts at: 493 1204 3837 493
Size: 711 2633 2702
Fragments arranged by size:
2702 2633 711
BsaBI GATnn'nnATC
Cuts at: 6040 6040
Size: 6046
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2441 3853 4235 4870
Size: 53 83 186 936 908 1412 382 635
Cuts at: 4870 275
Size: 1451
Fragments arranged by size:
1451 1412 936 908 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4479 4877 5151 5298 5709 4479
Size: 398 274 147 411 4816
Fragments arranged by size:
4816 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2519 2525 2549 2555 1178
Size: 30 165 30 1116 6 24 6 4669
Fragments arranged by size:
4669 1116 165 30 30 24 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2285 2839 1186
Size: 1099 554 4393
Fragments arranged by size:
4393 1099 554
BseYI C'CCAG_C
Cuts at: 5249 5745 5249
Size: 496 5550
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3236 1163
Size: 573 1500 3973
Fragments arranged by size:
3973 1500 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2143 2884 3103 3496 4257 5285
Size: 275 186 142 741 219 393 761 1028
Cuts at: 5285 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 741 393 275 219 186
142
BsiHKAI G_wGCw'C
Cuts at: 3168 4107 4192 5263 3168
Size: 939 85 1071 3951
Fragments arranged by size:
3951 1071 939 85
BsmI GAATG_Cn'
Cuts at: 2785 3557 3650 2785
Size: 772 93 5181
Fragments arranged by size:
5181 772 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2495 2741 3168 3365 4107 4192 5263
Size: 399 705 246 427 197 742 85 1071
Cuts at: 5263 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 705 427 399 246 197
85
BspEI T'CCGG_A
Cuts at: 5709 5709
Size: 6046
BspHI T'CATG_A
Cuts at: 3063 3935 5665 3063
Size: 872 1730 3444
Fragments arranged by size:
3444 1730 872
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2440 2592 3401 844
Size: 1596 152 809 3489
Fragments arranged by size:
3489 1596 809 152
BsrBI CCG'CTC
Cuts at: 1394 1823 2826 3933 1394
Size: 429 1003 1107 3507
Fragments arranged by size:
3507 1107 1003 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3282 4541 4715 65
Size: 1183 2034 1259 174 1396
Fragments arranged by size:
2034 1396 1259 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2647 3201 4690 4877 1700
Size: 110 837 554 1489 187 2869
Fragments arranged by size:
2869 1489 837 554 187 110
BsrGI T'GTAC_A
Cuts at: 96 2211 3309 96
Size: 2115 1098 2833
Fragments arranged by size:
2833 2115 1098
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6046
BssSI C'ACGA_G
Cuts at: 2231 4106 5118 2231
Size: 1875 1012 3159
Fragments arranged by size:
3159 1875 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6046
BstBI TT'CG_AA
Cuts at: 4850 4850
Size: 6046
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6046
BstYI r'GATC_y
Cuts at: 1972 2473 2502 3246 3376 3743 4128 4145 5597
Size: 501 29 744 130 367 385 17 1452
Cuts at: 5597 5683 5695 6041 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 744 501 385 367 346 130
86 29 17 12
Bsu36I CC'TnA_GG
Cuts at: 2808 2808
Size: 6046
BtgI C'CryG_G
Cuts at: 513 1065 2004 2433 2469 3173 513
Size: 552 939 429 36 704 3386
Fragments arranged by size:
3386 939 704 552 429 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3274 487
Size: 683 56 2048 3259
Fragments arranged by size:
3259 2048 683 56
BtsI GCAGTG_nn'
Cuts at: 2444 2704 3562 4356 4376 2444
Size: 260 858 794 20 4114
Fragments arranged by size:
4114 858 794 260 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6046
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6011
DraI TTT'AAA
Cuts at: 3350 3350
Size: 6046
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2569 5053 809
Size: 507 1253 2484 1802
Fragments arranged by size:
2484 1802 1253 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2121 2140 2515
Size: 54 1425 50 275 18 291 19 375
Cuts at: 2515 2607 2649 2881 3493 4382 6011 8
Size: 92 42 232 612 889 1629 43
Fragments arranged by size:
1629 1425 889 612 375 291 275 232
92 54 50 43 42 19 18
EagI C'GGCC_G
Cuts at: 1537 1812 2140 2881 3493 1537
Size: 275 328 741 612 4090
Fragments arranged by size:
4090 741 612 328 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2403 2720 2796 3976 1353
Size: 1050 317 76 1180 3423
Fragments arranged by size:
3423 1180 1050 317 76
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2591 4633 5007 5153 387
Size: 1064 138 1002 2042 374 146 1280
Fragments arranged by size:
2042 1280 1064 1002 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2055 2282 2420 2483 3149 4109 4706
Size: 393 423 227 138 63 666 960 597
Cuts at: 4706 5493 5761 1239
Size: 787 268 1524
Fragments arranged by size:
1524 960 787 666 597 423 393 268
227 138 63
EcoNI CCTnn'n_nnAGG
Cuts at: 2669 2669
Size: 6046
EcoO109I rG'GnC_Cy
Cuts at: 2425 2662 2812 2926 3072 2425
Size: 237 150 114 146 5399
Fragments arranged by size:
5399 237 150 146 114
EcoRI G'AATT_C
Cuts at: 3355 3733 3355
Size: 378 5668
FspI TGC'GCA
Cuts at: 2588 4552 2588
Size: 1964 4082
HaeII r_GCGC'y
Cuts at: 1678 3202 5193 1678
Size: 1524 1991 2531
Fragments arranged by size:
2531 1991 1524
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2069 2101 4687 4719 4761
Size: 32 135 32 497 32 2586 32 42
Cuts at: 4761 4793 1373
Size: 32 2626
Fragments arranged by size:
2626 2586 497 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2548 3390 3636 669
Size: 988 281 610 842 246 3079
Fragments arranged by size:
3079 988 842 610 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6046
HpaI GTT'AAC
Cuts at: 3636 3636
Size: 6046
KpnI G_GTAC'C
Cuts at: 3371 3371
Size: 6046
MfeI C'AATT_G
Cuts at: 3645 3645
Size: 6046
MluI A'CGCG_T
Cuts at: 5922 5922
Size: 6046
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2883 5160 5344 1305
Size: 1578 2277 184 2007
Fragments arranged by size:
2277 2007 1578 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2123 2517 2609 10
Size: 54 1425 343 291 394 92 3447
Fragments arranged by size:
3447 1425 394 343 291 92 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3218 4004 4363 4522 518
Size: 648 2052 786 359 159 2042
Fragments arranged by size:
2052 2042 786 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2075 2161 2768 2880 3462 4139 5287 5532
Size: 469 86 607 112 582 677 1148 245
Cuts at: 5532 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 607 582 469 245 112
86
NaeI GCC'GGC
Cuts at: 2649 3203 2649
Size: 554 5492
NcoI C'CATG_G
Cuts at: 513 1065 2004 2469 513
Size: 552 939 465 4090
Fragments arranged by size:
4090 939 552 465
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6046
NgoMIV G'CCGG_C
Cuts at: 2647 3201 2647
Size: 554 5492
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6046
NotI GC'GGCC_GC
Cuts at: 2881 3493 2881
Size: 612 5434
NspI r_CATG'y
Cuts at: 1132 1229 2572 2761 2893 3097 3343 3404 4949
Size: 97 1343 189 132 204 246 61 1545
Cuts at: 4949 1132
Size: 2229
Fragments arranged by size:
2229 1545 1343 246 204 189 132 97
61
PasI CC'CwG_GG
Cuts at: 1348 2055 1348
Size: 707 5339
PciI A'CATG_T
Cuts at: 2568 4945 2568
Size: 2377 3669
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6046
PfoI T'CCnGG_A
Cuts at: 2040 3318 2040
Size: 1278 4768
PmeI GTTT'AAAC
Cuts at: 3350 3350
Size: 6046
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4115 4147 5628 5660 4115
Size: 32 1481 32 4501
Fragments arranged by size:
4501 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2425 2662 2812 2926 2425
Size: 237 150 114 5545
Fragments arranged by size:
5545 237 150 114
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6046
PsiI TTA'TAA
Cuts at: 3616 3616
Size: 6046
PspXI vC'TCGA_Gb
Cuts at: 3136 3136
Size: 6046
PstI C_TGCA'G
Cuts at: 830 2451 3398 830
Size: 1621 947 3478
Fragments arranged by size:
3478 1621 947
PvuI CG_AT'CG
Cuts at: 2001 3103 2001
Size: 1102 4944
PvuII CAG'CTG
Cuts at: 2075 2768 2075
Size: 693 5353
SalI G'TCGA_C
Cuts at: 1936 3388 1936
Size: 1452 4594
SapI GCTCTTCn'nnn_
Cuts at: 2796 2796
Size: 6046
SbfI CC_TGCA'GG
Cuts at: 3398 3398
Size: 6046
ScaI AGT'ACT
Cuts at: 4294 6006 4294
Size: 1712 4334
SexAI A'CCwGG_T
Cuts at: 2961 2961
Size: 6046
SfcI C'TryA_G
Cuts at: 826 1045 2447 2722 3394 4529 5210 5401 826
Size: 219 1402 275 672 1135 681 191 1471
Fragments arranged by size:
1471 1402 1135 681 672 275 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6046
SgrAI Cr'CCGG_yG
Cuts at: 1700 3201 1700
Size: 1501 4545
SmaI CCC'GGG
Cuts at: 1872 2900 3158 3373 1872
Size: 1028 258 215 4545
Fragments arranged by size:
4545 1028 258 215
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2252 2420 3136 3167 4150 5051
Size: 197 926 309 168 716 31 983 901
Cuts at: 5051 5313 820
Size: 262 1553
Fragments arranged by size:
1553 983 926 901 716 309 262 197
168 31
SnaBI TAC'GTA
Cuts at: 493 3837 493
Size: 3344 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6046
SphI G_CATG'C
Cuts at: 1132 3404 1132
Size: 2272 3774
SspI AAT'ATT
Cuts at: 5 52 3970 5
Size: 47 3918 2081
Fragments arranged by size:
3918 2081 47
StuI AGG'CCT
Cuts at: 1517 3134 1517
Size: 1617 4429
StyI C'CwwG_G
Cuts at: 513 1065 2004 2469 3485 513
Size: 552 939 465 1016 3074
Fragments arranged by size:
3074 1016 939 552 465
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4073 4090 4243 4428 4873 325
Size: 1255 2493 17 153 185 445 1498
Fragments arranged by size:
2493 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2211 3309 4292
Size: 274 80 33 51 1443 234 1098 983
Cuts at: 4292 6004 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1098 983 274 234 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2519 2551 6006 6038 2519
Size: 32 3455 32 2527
Fragments arranged by size:
3455 2527 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3056 1316
Size: 1740 4306
XbaI T'CTAG_A
Cuts at: 3382 3382
Size: 6046
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2476 2476
Size: 6046
XhoI C'TCGA_G
Cuts at: 1943 3136 1943
Size: 1193 4853
XmaI C'CCGG_G
Cuts at: 1870 2898 3156 3371 1870
Size: 1028 258 215 4545
Fragments arranged by size:
4545 1028 258 215
XmnI GAAnn'nnTTC
Cuts at: 4175 4175
Size: 6046
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2442 3854 276
Size: 53 83 186 936 908 1412 2468
Fragments arranged by size:
2468 1412 936 908 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspXI PstI PvuI PvuII SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PmlI PspOMI PsrI RsrII SacI SacII
SanDI SfiI SfoI SrfI SwaI