Restriction map for
pFN21AB8129
(Circular) (Six-base) MAPSORT of: pFN21AB8129.seq Check: 2930 from: 1 to: 5728
pFN21AB8129 5728 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2104 2104
Size: 5728
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3538 278
Size: 53 83 186 936 2002 2468
Fragments arranged by size:
2468 2002 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2773 3071 1937
Size: 836 298 4594
Fragments arranged by size:
4594 836 298
Acc65I G'GTAC_C
Cuts at: 3049 3049
Size: 5728
AclI AA'CG_TT
Cuts at: 1563 3855 4228 1563
Size: 2292 373 3063
Fragments arranged by size:
3063 2292 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2103 2522 3791 5175 5443 1632
Size: 471 419 1269 1384 268 1917
Fragments arranged by size:
1917 1384 1269 471 419 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5531
AflIII A'CryG_T
Cuts at: 1203 2765 4627 5604 1203
Size: 1562 1862 977 1327
Fragments arranged by size:
1862 1562 1327 977
AgeI A'CCGG_T
Cuts at: 4559 4559
Size: 5728
AhdI GACnn_n'nnGTC
Cuts at: 2769 4457 2769
Size: 1688 4040
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5728
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5728
AlwNI CAG_nnn'CTG
Cuts at: 2204 2899 2962 2999 3471 5043 2204
Size: 695 63 37 472 1572 2889
Fragments arranged by size:
2889 1572 695 472 63 37
ApaLI G'TGCA_C
Cuts at: 2320 3785 4941 2320
Size: 1465 1156 3107
Fragments arranged by size:
3107 1465 1156
ApoI r'AATT_y
Cuts at: 1493 2546 3037 3263 3415 5524 1493
Size: 1053 491 226 152 2109 1697
Fragments arranged by size:
2109 1697 1053 491 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4282 160
Size: 872 3250 1606
Fragments arranged by size:
3250 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5728
AvaI C'yCGr_G
Cuts at: 1870 1943 2259 2751 3053 1870
Size: 73 316 492 302 4545
Fragments arranged by size:
4545 492 316 302 73
AvrII C'CTAG_G
Cuts at: 2020 2020
Size: 5728
BamHI G'GATC_C
Cuts at: 2016 3058 3425 2016
Size: 1042 367 4319
Fragments arranged by size:
4319 1042 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3049 4504 618
Size: 325 217 627 1262 1455 1842
Fragments arranged by size:
1842 1455 1262 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2177 3047 1391
Size: 786 870 4072
Fragments arranged by size:
4072 870 786
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2667 928
Size: 971 768 3989
Fragments arranged by size:
3989 971 768
BbvCI CC'TCA_GC
Cuts at: 2516 2516
Size: 5728
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3919 3953 1903
Size: 34 1982 34 3678
Fragments arranged by size:
3678 1982 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3620 4830 3620
Size: 1210 4518
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5728
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4339 136
Size: 107 122 71 631 605 2667 1525
Fragments arranged by size:
2667 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5723 5723
Size: 5728
BlpI GC'TnA_GC
Cuts at: 3145 3145
Size: 5728
Bme1580I G_kGCm'C
Cuts at: 1790 2324 2452 2788 3789 4945 1790
Size: 534 128 336 1001 1156 2573
Fragments arranged by size:
2573 1156 1001 534 336 128
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5728
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2504 3459 4417
Size: 416 53 83 399 24 1087 955 958
Cuts at: 4417 442
Size: 1753
Fragments arranged by size:
1753 1087 958 955 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5728
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3025 3025
Size: 5728
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2093 2117 2265 2292 2396 2972 2976 4388
Size: 854 24 148 27 104 576 4 1412
Cuts at: 4388 1239
Size: 2579
Fragments arranged by size:
2579 1412 854 576 148 104 27 24
4
Bpu10I CC'TnA_GC
Cuts at: 2516 2516
Size: 5728
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2798 2837 3853 4718 5016 2798
Size: 39 1016 865 298 3510
Fragments arranged by size:
3510 1016 865 298 39
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4391 882
Size: 617 2892 2219
Fragments arranged by size:
2892 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2768 3519 493
Size: 711 1564 751 2702
Fragments arranged by size:
2702 1564 751 711
BsaBI GATnn'nnATC
Cuts at: 2048 5722 2048
Size: 3674 2054
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3535 3917 4552 275
Size: 53 83 186 936 2002 382 635 1451
Fragments arranged by size:
2002 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4161 4559 4833 4980 5391 4161
Size: 398 274 147 411 4498
Fragments arranged by size:
4498 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2238 2268 1178
Size: 30 165 30 835 30 4638
Fragments arranged by size:
4638 835 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2747 3035 1186
Size: 1561 288 3879
Fragments arranged by size:
3879 1561 288
BseYI C'CCAG_C
Cuts at: 2440 2484 2933 4931 5427 2440
Size: 44 449 1998 496 2741
Fragments arranged by size:
2741 1998 496 449 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5155
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3178 3939 4967 1540
Size: 275 186 1177 761 1028 2301
Fragments arranged by size:
2301 1177 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2324 3789 3874 4945 2324
Size: 1465 85 1071 3107
Fragments arranged by size:
3107 1465 1071 85
BsmI GAATG_Cn'
Cuts at: 3239 3332 3239
Size: 93 5635
BsmBI CGTCTCn'nnnn_
Cuts at: 2402 2402
Size: 5728
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2177 2324 2452 2788 3047 3789 3874
Size: 399 387 147 128 336 259 742 85
Cuts at: 3874 4945 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 387 336 259 147
128 85
BspEI T'CCGG_A
Cuts at: 5391 5391
Size: 5728
BspHI T'CATG_A
Cuts at: 2670 3617 5347 2670
Size: 947 1730 3051
Fragments arranged by size:
3051 1730 947
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2104 3016 3083 844
Size: 1260 912 67 3489
Fragments arranged by size:
3489 1260 912 67
BsrBI CCG'CTC
Cuts at: 1394 1823 3615 1394
Size: 429 1792 3507
Fragments arranged by size:
3507 1792 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2265 4223 4397 65
Size: 1183 1017 1958 174 1396
Fragments arranged by size:
1958 1396 1183 1017 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4372 4559 1700
Size: 110 2562 187 2869
Fragments arranged by size:
2869 2562 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5728
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5728
BssSI C'ACGA_G
Cuts at: 3788 4800 3788
Size: 1012 4716
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2899 2962 794
Size: 2105 63 3560
Fragments arranged by size:
3560 2105 63
BstBI TT'CG_AA
Cuts at: 4532 4532
Size: 5728
BstEII G'GTnAC_C
Cuts at: 1184 2644 1184
Size: 1460 4268
BstXI CCAn_nnnn'nTGG
Cuts at: 2656 2656
Size: 5728
BstYI r'GATC_y
Cuts at: 1972 2016 2043 3058 3425 3810 3827 5279 5365
Size: 44 27 1015 367 385 17 1452 86
Cuts at: 5365 5377 5723 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1015 385 367 346 86 44
27 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2053 2655 513
Size: 552 988 602 3586
Fragments arranged by size:
3586 988 602 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4989
Fragments arranged by size:
4989 683 56
BtsI GCAGTG_nn'
Cuts at: 3244 4038 4058 3244
Size: 794 20 4914
Fragments arranged by size:
4914 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5728
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5693
DraI TTT'AAA
Cuts at: 3032 3032
Size: 5728
DraIII CAC_nnn'GTG
Cuts at: 2498 2498
Size: 5728
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4735 809
Size: 507 3419 1802
Fragments arranged by size:
3419 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2206 2841 3175
Size: 54 1425 50 275 18 376 635 334
Cuts at: 3175 4064 5693 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 635 376 334 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3175 1537
Size: 275 1363 4090
Fragments arranged by size:
4090 1363 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2909 3658 1353
Size: 1556 749 3423
Fragments arranged by size:
3423 1556 749
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2911 4315 4689 4835 387
Size: 1064 138 1322 1404 374 146 1280
Fragments arranged by size:
1404 1322 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2093 2103 2117 2265 2292 2396 2522
Size: 393 461 10 14 148 27 104 126
Cuts at: 2522 2972 2976 3791 4388 5175 5443 1239
Size: 450 4 815 597 787 268 1524
Fragments arranged by size:
1524 815 787 597 461 450 393 268
148 126 104 27 14 10 4
EcoNI CCTnn'n_nnAGG
Cuts at: 2024 2111 2024
Size: 87 5641
EcoO109I rG'GnC_Cy
Cuts at: 2104 2129 2747 2104
Size: 25 618 5085
Fragments arranged by size:
5085 618 25
EcoRI G'AATT_C
Cuts at: 3037 3415 3037
Size: 378 5350
FspI TGC'GCA
Cuts at: 4234 4234
Size: 5728
HaeII r_GCGC'y
Cuts at: 1678 4875 1678
Size: 3197 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2121 2153 2755 2787 4369
Size: 32 135 32 549 32 602 32 1582
Cuts at: 4369 4401 4443 4475 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1582 602 549 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2774 3072 3318 669
Size: 988 281 836 298 246 3079
Fragments arranged by size:
3079 988 836 298 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5728
HpaI GTT'AAC
Cuts at: 3318 3318
Size: 5728
KpnI G_GTAC'C
Cuts at: 3053 3053
Size: 5728
MfeI C'AATT_G
Cuts at: 3327 3327
Size: 5728
MluI A'CGCG_T
Cuts at: 5604 5604
Size: 5728
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4842 5026 1305
Size: 3537 184 2007
Fragments arranged by size:
3537 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2843 10
Size: 54 1425 343 1011 2895
Fragments arranged by size:
2895 1425 1011 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2669 2675 3686 4045 4204 518
Size: 648 1503 6 1011 359 159 2042
Fragments arranged by size:
2042 1503 1011 648 359 159 6
MspA1I CmG'CkG
Cuts at: 1606 2440 2896 2950 3144 3821 4969 5214 1606
Size: 834 456 54 194 677 1148 245 2120
Fragments arranged by size:
2120 1148 834 677 456 245 194 54
NcoI C'CATG_G
Cuts at: 513 1065 2655 513
Size: 552 1590 3586
Fragments arranged by size:
3586 1590 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5728
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5728
NotI GC'GGCC_GC
Cuts at: 3175 3175
Size: 5728
NspI r_CATG'y
Cuts at: 1132 1229 2492 3086 4631 1132
Size: 97 1263 594 1545 2229
Fragments arranged by size:
2229 1545 1263 594 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5728
PciI A'CATG_T
Cuts at: 4627 4627
Size: 5728
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5728
PfoI T'CCnGG_A
Cuts at: 2859 2992 2859
Size: 133 5595
PmeI GTTT'AAAC
Cuts at: 3032 3032
Size: 5728
PmlI CAC'GTG
Cuts at: 2768 2768
Size: 5728
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3797 3829 5310 5342 3797
Size: 32 1481 32 4183
Fragments arranged by size:
4183 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2104 2129 2104
Size: 25 5703
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5728
PsiI TTA'TAA
Cuts at: 3298 3298
Size: 5728
PstI C_TGCA'G
Cuts at: 830 2391 2439 2481 2967 3080 830
Size: 1561 48 42 486 113 3478
Fragments arranged by size:
3478 1561 486 113 48 42
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5728
PvuII CAG'CTG
Cuts at: 2440 2896 2950 2440
Size: 456 54 5218
Fragments arranged by size:
5218 456 54
SalI G'TCGA_C
Cuts at: 1936 2772 3070 1936
Size: 836 298 4594
Fragments arranged by size:
4594 836 298
SapI GCTCTTCn'nnn_
Cuts at: 2909 2909
Size: 5728
SbfI CC_TGCA'GG
Cuts at: 3080 3080
Size: 5728
ScaI AGT'ACT
Cuts at: 3976 5688 3976
Size: 1712 4016
SfcI C'TryA_G
Cuts at: 826 1045 2387 2435 2467 2477 2963 3076 4211
Size: 219 1342 48 32 10 486 113 1135
Cuts at: 4211 4892 5083 826
Size: 681 191 1471
Fragments arranged by size:
1471 1342 1135 681 486 219 191 113
48 32 10
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5728
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5728
SmaI CCC'GGG
Cuts at: 1872 2753 3055 1872
Size: 881 302 4545
Fragments arranged by size:
4545 881 302
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2777 2852 3832 4733 4995 820
Size: 197 926 834 75 980 901 262 1553
Fragments arranged by size:
1553 980 926 901 834 262 197 75
SnaBI TAC'GTA
Cuts at: 493 3519 493
Size: 3026 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5728
SphI G_CATG'C
Cuts at: 1132 2492 3086 1132
Size: 1360 594 3774
Fragments arranged by size:
3774 1360 594
SspI AAT'ATT
Cuts at: 5 52 3652 5
Size: 47 3600 2081
Fragments arranged by size:
3600 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5728
StyI C'CwwG_G
Cuts at: 513 1065 2020 2655 3167 513
Size: 552 955 635 512 3074
Fragments arranged by size:
3074 955 635 552 512
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2486 3755 3772 3925 4110 4555 325
Size: 1255 906 1269 17 153 185 445 1498
Fragments arranged by size:
1498 1269 1255 906 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3974 5686 96
Size: 274 80 33 51 1443 1997 1712 138
Fragments arranged by size:
1997 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2238 2270 5688 5720 2238
Size: 32 3418 32 2246
Fragments arranged by size:
3418 2246 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2288 1316
Size: 972 4756
XbaI T'CTAG_A
Cuts at: 3064 3064
Size: 5728
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5728
XmaI C'CCGG_G
Cuts at: 1870 2751 3053 1870
Size: 881 302 4545
Fragments arranged by size:
4545 881 302
XmnI GAAnn'nnTTC
Cuts at: 3857 3857
Size: 5728
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3536 276
Size: 53 83 186 936 2002 2468
Fragments arranged by size:
2468 2002 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbeI BfrBI BsiWI
BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI