Restriction map for
pFN21AB8127
(Circular) (Six-base) MAPSORT of: pFN21AB8127.seq Check: 4808 from: 1 to: 6058
pFN21AB8127 6058 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2154 3868 278
Size: 53 83 186 936 618 1714 2468
Fragments arranged by size:
2468 1714 936 618 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3401 1937
Size: 1464 4594
Acc65I G'GTAC_C
Cuts at: 2484 3379 2484
Size: 895 5163
AclI AA'CG_TT
Cuts at: 1563 4185 4558 1563
Size: 2622 373 3063
Fragments arranged by size:
3063 2622 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4121 5505 5773 1632
Size: 2489 1384 268 1917
Fragments arranged by size:
2489 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5861
AflIII A'CryG_T
Cuts at: 1203 3240 4957 5934 1203
Size: 2037 1717 977 1327
Fragments arranged by size:
2037 1717 1327 977
AgeI A'CCGG_T
Cuts at: 4889 4889
Size: 6058
AhdI GACnn_n'nnGTC
Cuts at: 4787 4787
Size: 6058
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6058
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6058
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3227 3259 3227
Size: 32 6026
AlwNI CAG_nnn'CTG
Cuts at: 3801 5373 3801
Size: 1572 4486
ApaI G_GGCC'C
Cuts at: 3062 3062
Size: 6058
ApaLI G'TGCA_C
Cuts at: 4115 5271 4115
Size: 1156 4902
ApoI r'AATT_y
Cuts at: 1493 2343 3367 3593 3745 5854 1493
Size: 850 1024 226 152 2109 1697
Fragments arranged by size:
2109 1697 1024 850 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4612 160
Size: 872 3580 1606
Fragments arranged by size:
3580 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6058
AvaI C'yCGr_G
Cuts at: 1870 1943 2010 2102 2190 2217 3218 3383 1870
Size: 73 67 92 88 27 1001 165 4545
Fragments arranged by size:
4545 1001 165 92 88 73 67 27
BamHI G'GATC_C
Cuts at: 3021 3388 3755 3021
Size: 367 367 5324
Fragments arranged by size:
5324 367 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2484 3344 3379 4834 618
Size: 325 217 627 697 860 35 1455 1842
Fragments arranged by size:
1842 1455 860 697 627 325 217 35
BanII G_rGCy'C
Cuts at: 1391 2456 3062 3132 3297 3377 1391
Size: 1065 606 70 165 80 4072
Fragments arranged by size:
4072 1065 606 165 80 70
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2358 2870 928
Size: 971 459 512 4116
Fragments arranged by size:
4116 971 512 459
BbvCI CC'TCA_GC
Cuts at: 3109 3109
Size: 6058
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4249 4283 1903
Size: 34 50 34 2228 34 3678
Fragments arranged by size:
3678 2228 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3950 5160 3950
Size: 1210 4848
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6058
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2043 2106 4669
Size: 107 122 71 631 605 371 63 2563
Cuts at: 4669 136
Size: 1525
Fragments arranged by size:
2563 1525 631 605 371 122 107 71
63
BglII A'GATC_T
Cuts at: 6053 6053
Size: 6058
BlpI GC'TnA_GC
Cuts at: 3475 3475
Size: 6058
Bme1580I G_kGCm'C
Cuts at: 1790 2138 3062 3347 4119 5275 1790
Size: 348 924 285 772 1156 2573
Fragments arranged by size:
2573 1156 924 772 348 285
BmgBI CAC'GTC
Cuts at: 1318 3241 1318
Size: 1923 4135
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2323 3789 4747
Size: 416 53 83 399 24 906 1466 958
Cuts at: 4747 442
Size: 1753
Fragments arranged by size:
1753 1466 958 906 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6058
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3111 3111
Size: 6058
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2486 4718 1239
Size: 1247 2232 2579
Fragments arranged by size:
2579 2232 1247
Bpu10I CC'TnA_GC
Cuts at: 3109 3109
Size: 6058
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2564 4183 5048 5346 2564
Size: 1619 865 298 3276
Fragments arranged by size:
3276 1619 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2925 4721 882
Size: 617 1426 1796 2219
Fragments arranged by size:
2219 1796 1426 617
BsaAI yAC'GTr
Cuts at: 493 1204 3849 493
Size: 711 2645 2702
Fragments arranged by size:
2702 2645 711
BsaBI GATnn'nnATC
Cuts at: 6052 6052
Size: 6058
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2151 3865 4247 4882
Size: 53 83 186 936 618 1714 382 635
Cuts at: 4882 275
Size: 1451
Fragments arranged by size:
1714 1451 936 635 618 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2637 4491 4889 5163 5310 5721 2637
Size: 1854 398 274 147 411 2974
Fragments arranged by size:
2974 1854 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3227 3257 1178
Size: 30 165 30 1824 30 3979
Fragments arranged by size:
3979 1824 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2132 2310 2313 3148 1186
Size: 946 178 3 835 4096
Fragments arranged by size:
4096 946 835 178 3
BseYI C'CCAG_C
Cuts at: 2032 2429 3321 5261 5757 2032
Size: 397 892 1940 496 2333
Fragments arranged by size:
2333 1940 892 496 397
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2522 2642 1163
Size: 573 786 120 4579
Fragments arranged by size:
4579 786 573 120
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3508 4269 5297 1540
Size: 275 186 1507 761 1028 2301
Fragments arranged by size:
2301 1507 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2181 3297 4119 4204 5275 2181
Size: 1116 822 85 1071 2964
Fragments arranged by size:
2964 1116 1071 822 85
BsmI GAATG_Cn'
Cuts at: 3569 3662 3569
Size: 93 5965
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2138 2181 2456 3062 3132 3297 3347
Size: 399 348 43 275 606 70 165 50
Cuts at: 3347 3377 4119 4204 5275 1391
Size: 30 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 606 399 348 275 165
85 70 50 43 30
BspEI T'CCGG_A
Cuts at: 5721 5721
Size: 6058
BspHI T'CATG_A
Cuts at: 2347 2811 2883 3132 3947 5677 2347
Size: 464 72 249 815 1730 2728
Fragments arranged by size:
2728 1730 815 464 249 72
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3111 3346 3413 844
Size: 2267 235 67 3489
Fragments arranged by size:
3489 2267 235 67
BsrBI CCG'CTC
Cuts at: 1394 1823 2830 3945 1394
Size: 429 1007 1115 3507
Fragments arranged by size:
3507 1115 1007 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4553 4727 65
Size: 1183 3305 174 1396
Fragments arranged by size:
3305 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2816 4702 4889 1700
Size: 110 1006 1886 187 2869
Fragments arranged by size:
2869 1886 1006 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6058
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6058
BssSI C'ACGA_G
Cuts at: 4118 5130 4118
Size: 1012 5046
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6058
BstBI TT'CG_AA
Cuts at: 4862 4862
Size: 6058
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6058
BstXI CCAn_nnnn'nTGG
Cuts at: 2875 2875
Size: 6058
BstYI r'GATC_y
Cuts at: 1972 3021 3388 3755 4140 4157 5609 5695 5707
Size: 1049 367 367 385 17 1452 86 12
Cuts at: 5707 6053 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1049 385 367 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2795 2868 3053 513
Size: 552 1730 73 185 3518
Fragments arranged by size:
3518 1730 552 185 73
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2410 2758 2941 487
Size: 683 56 1184 348 183 3604
Fragments arranged by size:
3604 1184 683 348 183 56
BtsI GCAGTG_nn'
Cuts at: 2947 3574 4368 4388 2947
Size: 627 794 20 4617
Fragments arranged by size:
4617 794 627 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6058
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6023
DraI TTT'AAA
Cuts at: 3362 3362
Size: 6058
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5065 809
Size: 507 3749 1802
Fragments arranged by size:
3749 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2715 2850 3039
Size: 54 1425 50 275 18 885 135 189
Cuts at: 3039 3138 3315 3505 4394 6023 8
Size: 99 177 190 889 1629 43
Fragments arranged by size:
1629 1425 889 885 275 190 189 177
135 99 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3505 1537
Size: 275 1693 4090
Fragments arranged by size:
4090 1693 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3988 1353
Size: 2635 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4645 5019 5165 387
Size: 1064 138 3056 374 146 1280
Fragments arranged by size:
3056 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3295 3295
Size: 6058
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2486 4121 4718 5505 5773 1239
Size: 393 854 1635 597 787 268 1524
Fragments arranged by size:
1635 1524 854 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 3058 3059 3058
Size: 1 6057
EcoRI G'AATT_C
Cuts at: 3367 3745 3367
Size: 378 5680
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2170 3271 2170
Size: 1101 4957
FspI TGC'GCA
Cuts at: 4564 4564
Size: 6058
HaeII r_GCGC'y
Cuts at: 1678 5205 1678
Size: 3527 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4699 4731 4773 4805 1373
Size: 32 135 32 3127 32 42 32 2626
Fragments arranged by size:
3127 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3402 3648 669
Size: 988 281 1464 246 3079
Fragments arranged by size:
3079 1464 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6058
HpaI GTT'AAC
Cuts at: 3648 3648
Size: 6058
KpnI G_GTAC'C
Cuts at: 2488 3383 2488
Size: 895 5163
MfeI C'AATT_G
Cuts at: 3657 3657
Size: 6058
MluI A'CGCG_T
Cuts at: 5934 5934
Size: 6058
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2265 5172 5356 1305
Size: 960 2907 184 2007
Fragments arranged by size:
2907 2007 960 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2852 3041 3140 10
Size: 54 1425 343 1020 189 99 2928
Fragments arranged by size:
2928 1425 1020 343 189 99 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2708 3074 4016 4375 4534 518
Size: 648 1542 366 942 359 159 2042
Fragments arranged by size:
2042 1542 942 648 366 359 159
MspA1I CmG'CkG
Cuts at: 1606 2032 2077 2322 2695 3126 3474 4151 5299
Size: 426 45 245 373 431 348 677 1148
Cuts at: 5299 5544 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 431 426 373 348 245
245 45
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5506
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6058
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6058
NotI GC'GGCC_GC
Cuts at: 3505 3505
Size: 6058
NspI r_CATG'y
Cuts at: 1132 1229 3416 4961 1132
Size: 97 2187 1545 2229
Fragments arranged by size:
2229 2187 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2332 1348
Size: 984 5074
PciI A'CATG_T
Cuts at: 4957 4957
Size: 6058
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2848 1108
Size: 1740 4318
PfoI T'CCnGG_A
Cuts at: 2463 2874 3024 2463
Size: 411 150 5497
Fragments arranged by size:
5497 411 150
PmeI GTTT'AAAC
Cuts at: 3362 3362
Size: 6058
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3227 3259 4127 4159 5640 5672 3227
Size: 32 868 32 1481 32 3613
Fragments arranged by size:
3613 1481 868 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2870 1613
Size: 1257 4801
PsiI TTA'TAA
Cuts at: 3628 3628
Size: 6058
PspOMI G'GGCC_C
Cuts at: 3058 3058
Size: 6058
PspXI vC'TCGA_Gb
Cuts at: 3218 3218
Size: 6058
PstI C_TGCA'G
Cuts at: 830 2031 2901 3018 3410 830
Size: 1201 870 117 392 3478
Fragments arranged by size:
3478 1201 870 392 117
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6058
PvuII CAG'CTG
Cuts at: 2032 2077 2695 2032
Size: 45 618 5395
Fragments arranged by size:
5395 618 45
SacI G_AGCT'C
Cuts at: 3297 3297
Size: 6058
SalI G'TCGA_C
Cuts at: 1936 3400 1936
Size: 1464 4594
SbfI CC_TGCA'GG
Cuts at: 3410 3410
Size: 6058
ScaI AGT'ACT
Cuts at: 4306 6018 4306
Size: 1712 4346
SfcI C'TryA_G
Cuts at: 826 1045 2027 2835 2897 3014 3406 4541 5222
Size: 219 982 808 62 117 392 1135 681
Cuts at: 5222 5413 826
Size: 191 1471
Fragments arranged by size:
1471 1135 982 808 681 392 219 191
117 62
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6058
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6058
SmaI CCC'GGG
Cuts at: 1872 2012 2192 2219 3385 1872
Size: 140 180 27 1166 4545
Fragments arranged by size:
4545 1166 180 140 27
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2579 3218 4162 5063 5325 820
Size: 197 926 636 639 944 901 262 1553
Fragments arranged by size:
1553 944 926 901 639 636 262 197
SnaBI TAC'GTA
Cuts at: 493 3849 493
Size: 3356 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6058
SphI G_CATG'C
Cuts at: 1132 3416 1132
Size: 2284 3774
SspI AAT'ATT
Cuts at: 5 52 3982 5
Size: 47 3930 2081
Fragments arranged by size:
3930 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6058
StyI C'CwwG_G
Cuts at: 513 1065 3497 513
Size: 552 2432 3074
Fragments arranged by size:
3074 2432 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2388 3011 3335 3362 4085 4102 4255
Size: 1255 808 623 324 27 723 17 153
Cuts at: 4255 4440 4885 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 808 723 623 445 324 185
153 27 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4304 6016 96
Size: 274 80 33 51 1443 2327 1712 138
Fragments arranged by size:
2327 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6018 6050 6018
Size: 32 6026
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6058
XbaI T'CTAG_A
Cuts at: 3394 3394
Size: 6058
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2911 3073 2911
Size: 162 5896
XhoI C'TCGA_G
Cuts at: 1943 3218 1943
Size: 1275 4783
XmaI C'CCGG_G
Cuts at: 1870 2010 2190 2217 3383 1870
Size: 140 180 27 1166 4545
Fragments arranged by size:
4545 1166 180 140 27
XmnI GAAnn'nnTTC
Cuts at: 4187 4187
Size: 6058
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2152 3866 276
Size: 53 83 186 936 618 1714 2468
Fragments arranged by size:
2468 1714 936 618 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PpuMI PsrI RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI