Restriction map for
pFN21AB8126
(Circular) (Six-base) MAPSORT of: pFN21AB8126.seq Check: 9939 from: 1 to: 6364
pFN21AB8126 6364 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4174 278
Size: 53 83 186 936 2638 2468
Fragments arranged by size:
2638 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3707 1937
Size: 1770 4594
Acc65I G'GTAC_C
Cuts at: 3296 3685 3296
Size: 389 5975
AclI AA'CG_TT
Cuts at: 1563 2695 3231 4491 4864 1563
Size: 1132 536 1260 373 3063
Fragments arranged by size:
3063 1260 1132 536 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2286 2843 3437 4427 5811 6079 1632
Size: 654 557 594 990 1384 268 1917
Fragments arranged by size:
1917 1384 990 654 594 557 268
AflII C'TTAA_G
Cuts at: 820 1017 2227 820
Size: 197 1210 4957
Fragments arranged by size:
4957 1210 197
AflIII A'CryG_T
Cuts at: 1203 2507 5263 6240 1203
Size: 1304 2756 977 1327
Fragments arranged by size:
2756 1327 1304 977
AgeI A'CCGG_T
Cuts at: 5195 5195
Size: 6364
AhdI GACnn_n'nnGTC
Cuts at: 5093 5093
Size: 6364
AleI CACnn'nnGTG
Cuts at: 1166 2822 1166
Size: 1656 4708
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6364
AlwNI CAG_nnn'CTG
Cuts at: 2866 4107 5679 2866
Size: 1241 1572 3551
Fragments arranged by size:
3551 1572 1241
ApaLI G'TGCA_C
Cuts at: 4421 5577 4421
Size: 1156 5208
ApoI r'AATT_y
Cuts at: 1493 2550 3640 3673 3899 4051 6160 1493
Size: 1057 1090 33 226 152 2109 1697
Fragments arranged by size:
2109 1697 1090 1057 226 152 33
AseI AT'TA_AT
Cuts at: 160 1032 4918 160
Size: 872 3886 1606
Fragments arranged by size:
3886 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6364
AvaI C'yCGr_G
Cuts at: 1870 1943 3689 1870
Size: 73 1746 4545
Fragments arranged by size:
4545 1746 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2184 2217 2846 2879 3196 3229 2184
Size: 33 629 33 317 33 5319
Fragments arranged by size:
5319 629 317 33 33 33
BamHI G'GATC_C
Cuts at: 3257 3694 4061 3257
Size: 437 367 5560
Fragments arranged by size:
5560 437 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2602 3296 3685 5140 618
Size: 325 217 627 815 694 389 1455 1842
Fragments arranged by size:
1842 1455 815 694 627 389 325 217
BanII G_rGCy'C
Cuts at: 1391 3507 3683 1391
Size: 2116 176 4072
Fragments arranged by size:
4072 2116 176
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5393
BbvCI CC'TCA_GC
Cuts at: 2264 2264
Size: 6364
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4555 4589 1903
Size: 34 50 34 2534 34 3678
Fragments arranged by size:
3678 2534 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3224 4256 5466 3224
Size: 1032 1210 4122
Fragments arranged by size:
4122 1210 1032
BclI T'GATC_A
Cuts at: 1548 2928 1548
Size: 1380 4984
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4975 136
Size: 107 122 71 631 605 3303 1525
Fragments arranged by size:
3303 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3180 3597 6359 3180
Size: 417 2762 3185
Fragments arranged by size:
3185 2762 417
BlpI GC'TnA_GC
Cuts at: 3781 3781
Size: 6364
Bme1580I G_kGCm'C
Cuts at: 1790 3578 4425 5581 1790
Size: 1788 847 1156 2573
Fragments arranged by size:
2573 1788 1156 847
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6364
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2151 2226 2661
Size: 416 53 83 399 24 734 75 435
Cuts at: 2661 2770 3340 4095 5053 442
Size: 109 570 755 958 1753
Fragments arranged by size:
1753 958 755 734 570 435 416 399
109 83 75 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6364
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2176 2176
Size: 6364
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2053 3071 3521 5024 1239
Size: 814 1018 450 1503 2579
Fragments arranged by size:
2579 1503 1018 814 450
Bpu10I CC'TnA_GC
Cuts at: 2264 2264
Size: 6364
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4489 5354 5652 4489
Size: 865 298 5201
Fragments arranged by size:
5201 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2071 5027 882
Size: 617 572 2956 2219
Fragments arranged by size:
2956 2219 617 572
BsaAI yAC'GTr
Cuts at: 493 1204 4155 493
Size: 711 2951 2702
Fragments arranged by size:
2951 2702 711
BsaBI GATnn'nnATC
Cuts at: 6358 6358
Size: 6364
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4171 4553 5188 275
Size: 53 83 186 936 2638 382 635 1451
Fragments arranged by size:
2638 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4797 5195 5469 5616 6027 4797
Size: 398 274 147 411 5134
Fragments arranged by size:
5134 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2379 2400 2409 2430 3023
Size: 30 165 30 976 21 9 21 593
Cuts at: 3023 3053 1178
Size: 30 4489
Fragments arranged by size:
4489 976 593 165 30 30 30 21
21 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2450 1186
Size: 1264 5100
BseYI C'CCAG_C
Cuts at: 3506 5567 6063 3506
Size: 2061 496 3807
Fragments arranged by size:
3807 2061 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5791
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2031 3814 4575 5603 1540
Size: 275 186 30 1783 761 1028 2301
Fragments arranged by size:
2301 1783 1028 761 275 186 30
BsiHKAI G_wGCw'C
Cuts at: 4425 4510 5581 4425
Size: 85 1071 5208
Fragments arranged by size:
5208 1071 85
BsmI GAATG_Cn'
Cuts at: 3535 3875 3968 3535
Size: 340 93 5931
Fragments arranged by size:
5931 340 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3507 3578 3683 4425 4510 5581 1391
Size: 399 1717 71 105 742 85 1071 2174
Fragments arranged by size:
2174 1717 1071 742 399 105 85 71
BspEI T'CCGG_A
Cuts at: 6027 6027
Size: 6364
BspHI T'CATG_A
Cuts at: 4253 5983 4253
Size: 1730 4634
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3277 3284 3719 844
Size: 2433 7 435 3489
Fragments arranged by size:
3489 2433 435 7
BsrBI CCG'CTC
Cuts at: 1394 1823 4251 1394
Size: 429 2428 3507
Fragments arranged by size:
3507 2428 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2094 4859 5033 65
Size: 1183 846 2765 174 1396
Fragments arranged by size:
2765 1396 1183 846 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5008 5195 1700
Size: 110 3198 187 2869
Fragments arranged by size:
3198 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6364
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6364
BssSI C'ACGA_G
Cuts at: 3249 4424 5436 3249
Size: 1175 1012 4177
Fragments arranged by size:
4177 1175 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6364
BstBI TT'CG_AA
Cuts at: 5168 5168
Size: 6364
BstEII G'GTnAC_C
Cuts at: 1184 2639 1184
Size: 1455 4909
BstXI CCAn_nnnn'nTGG
Cuts at: 3307 3307
Size: 6364
BstYI r'GATC_y
Cuts at: 1972 2708 2762 3180 3257 3597 3694 4061 4446
Size: 736 54 418 77 340 97 367 385
Cuts at: 4446 4463 5915 6001 6013 6359 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 736 418 385 367 346 340
97 86 77 54 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2031 2889 3438 513
Size: 552 966 858 549 3439
Fragments arranged by size:
3439 966 858 552 549
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5625
Fragments arranged by size:
5625 683 56
BtsI GCAGTG_nn'
Cuts at: 3880 4674 4694 3880
Size: 794 20 5550
Fragments arranged by size:
5550 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6364
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6329
DraI TTT'AAA
Cuts at: 3668 3668
Size: 6364
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3000 5371 809
Size: 507 1684 2371 1802
Fragments arranged by size:
2371 1802 1684 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2028 3811 4700
Size: 54 1425 50 275 18 198 1783 889
Cuts at: 4700 6329 8
Size: 1629 43
Fragments arranged by size:
1783 1629 1425 889 275 198 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2028 3811 1537
Size: 275 216 1783 4090
Fragments arranged by size:
4090 1783 275 216
EarI CTCTTCn'nnn_
Cuts at: 1353 2166 2393 2780 4294 1353
Size: 813 227 387 1514 3423
Fragments arranged by size:
3423 1514 813 387 227
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2978 3206 4951 5325 5471 387
Size: 1064 138 1389 228 1745 374 146 1280
Fragments arranged by size:
1745 1389 1280 1064 374 228 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2053 2286 2843 3071 3437 3521 4427
Size: 393 421 233 557 228 366 84 906
Cuts at: 4427 5024 5811 6079 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 906 787 597 557 421 393 366
268 233 228 84
EcoRI G'AATT_C
Cuts at: 3673 4051 3673
Size: 378 5986
EcoRV GAT'ATC
Cuts at: 3488 3488
Size: 6364
FspI TGC'GCA
Cuts at: 4870 4870
Size: 6364
HaeII r_GCGC'y
Cuts at: 1678 5511 1678
Size: 3833 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2930 2962 5005 5037 5079
Size: 32 135 32 1358 32 2043 32 42
Cuts at: 5079 5111 1373
Size: 32 2626
Fragments arranged by size:
2626 2043 1358 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3708 3954 669
Size: 988 281 1770 246 3079
Fragments arranged by size:
3079 1770 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6364
HpaI GTT'AAC
Cuts at: 3954 3954
Size: 6364
KpnI G_GTAC'C
Cuts at: 3300 3689 3300
Size: 389 5975
MfeI C'AATT_G
Cuts at: 2493 2607 2664 3963 2493
Size: 114 57 1299 4894
Fragments arranged by size:
4894 1299 114 57
MluI A'CGCG_T
Cuts at: 2507 6240 2507
Size: 3733 2631
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3173 3235 5478 5662 1305
Size: 1868 62 2243 184 2007
Fragments arranged by size:
2243 2007 1868 184 62
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4542
Fragments arranged by size:
4542 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2822 2825 4322 4681 4840 518
Size: 648 1656 3 1497 359 159 2042
Fragments arranged by size:
2042 1656 1497 648 359 159 3
MspA1I CmG'CkG
Cuts at: 1606 3263 3780 4457 5605 5850 1606
Size: 1657 517 677 1148 245 2120
Fragments arranged by size:
2120 1657 1148 677 517 245
NcoI C'CATG_G
Cuts at: 513 1065 2889 3438 513
Size: 552 1824 549 3439
Fragments arranged by size:
3439 1824 552 549
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6364
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6364
NotI GC'GGCC_GC
Cuts at: 3811 3811
Size: 6364
NspI r_CATG'y
Cuts at: 1132 1229 2924 3722 5267 1132
Size: 97 1695 798 1545 2229
Fragments arranged by size:
2229 1695 1545 798 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6364
PciI A'CATG_T
Cuts at: 5263 5263
Size: 6364
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6364
PmeI GTTT'AAAC
Cuts at: 3668 3668
Size: 6364
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4433 4465 5946 5978 4433
Size: 32 1481 32 4819
Fragments arranged by size:
4819 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6364
PsiI TTA'TAA
Cuts at: 3934 3934
Size: 6364
PstI C_TGCA'G
Cuts at: 830 3716 830
Size: 2886 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6364
SalI G'TCGA_C
Cuts at: 1936 3706 1936
Size: 1770 4594
SapI GCTCTTCn'nnn_
Cuts at: 2166 2166
Size: 6364
SbfI CC_TGCA'GG
Cuts at: 3716 3716
Size: 6364
ScaI AGT'ACT
Cuts at: 4612 6324 4612
Size: 1712 4652
SfcI C'TryA_G
Cuts at: 826 1045 3712 4847 5528 5719 826
Size: 219 2667 1135 681 191 1471
Fragments arranged by size:
2667 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6364
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6364
SmaI CCC'GGG
Cuts at: 1872 3691 1872
Size: 1819 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2227 4468 5369 5631 820
Size: 197 926 284 2241 901 262 1553
Fragments arranged by size:
2241 1553 926 901 284 262 197
SnaBI TAC'GTA
Cuts at: 493 4155 493
Size: 3662 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6364
SphI G_CATG'C
Cuts at: 1132 3722 1132
Size: 2590 3774
SspI AAT'ATT
Cuts at: 5 52 4288 5
Size: 47 4236 2081
Fragments arranged by size:
4236 2081 47
StuI AGG'CCT
Cuts at: 1517 3011 1517
Size: 1494 4870
StyI C'CwwG_G
Cuts at: 513 1065 2464 2889 3438 3803 513
Size: 552 1399 425 549 365 3074
Fragments arranged by size:
3074 1399 552 549 425 365
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2658 4391 4408 4561 4746 5191 325
Size: 1255 1078 1733 17 153 185 445 1498
Fragments arranged by size:
1733 1498 1255 1078 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2730 2814 4610
Size: 274 80 33 51 1443 753 84 1796
Cuts at: 4610 6322 96
Size: 1712 138
Fragments arranged by size:
1796 1712 1443 753 274 138 84 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2379 2400 2411 2432 6324 6356 2379
Size: 21 11 21 3892 32 2387
Fragments arranged by size:
3892 2387 32 21 21 11
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6364
XbaI T'CTAG_A
Cuts at: 3700 3700
Size: 6364
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2077 2077
Size: 6364
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6364
XmaI C'CCGG_G
Cuts at: 1870 3689 1870
Size: 1819 4545
XmnI GAAnn'nnTTC
Cuts at: 2696 3533 4493 2696
Size: 837 960 4567
Fragments arranged by size:
4567 960 837
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4172 276
Size: 53 83 186 936 2638 2468
Fragments arranged by size:
2638 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI