Restriction map for
pFN21AB8125
(Circular) (Six-base) MAPSORT of: pFN21AB8125.seq Check: 2286 from: 1 to: 6070
pFN21AB8125 6070 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2637 3880 278
Size: 53 83 186 936 1101 1243 2468
Fragments arranged by size:
2468 1243 1101 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2572 3261 3413 1937
Size: 635 689 152 4594
Fragments arranged by size:
4594 689 635 152
Acc65I G'GTAC_C
Cuts at: 3391 3391
Size: 6070
AclI AA'CG_TT
Cuts at: 1563 4197 4570 1563
Size: 2634 373 3063
Fragments arranged by size:
3063 2634 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2147 3315 4133 5517 5785 1632
Size: 515 1168 818 1384 268 1917
Fragments arranged by size:
1917 1384 1168 818 515 268
AflII C'TTAA_G
Cuts at: 820 1017 2869 820
Size: 197 1852 4021
Fragments arranged by size:
4021 1852 197
AflIII A'CryG_T
Cuts at: 1203 2773 4969 5946 1203
Size: 1570 2196 977 1327
Fragments arranged by size:
2196 1570 1327 977
AgeI A'CCGG_T
Cuts at: 4901 4901
Size: 6070
AhdI GACnn_n'nnGTC
Cuts at: 4799 4799
Size: 6070
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6070
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6070
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3315 3347 3315
Size: 32 6038
AlwNI CAG_nnn'CTG
Cuts at: 2056 3813 5385 2056
Size: 1757 1572 2741
Fragments arranged by size:
2741 1757 1572
ApaLI G'TGCA_C
Cuts at: 4127 5283 4127
Size: 1156 4914
ApoI r'AATT_y
Cuts at: 1493 2023 2059 2285 2564 3190 3379 3605 3757
Size: 530 36 226 279 626 189 226 152
Cuts at: 3757 5866 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 626 530 279 226 226 189
152 36
AseI AT'TA_AT
Cuts at: 160 1032 4624 160
Size: 872 3592 1606
Fragments arranged by size:
3592 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6070
AvaI C'yCGr_G
Cuts at: 1870 1943 2014 3395 1870
Size: 73 71 1381 4545
Fragments arranged by size:
4545 1381 73 71
AvrII C'CTAG_G
Cuts at: 2825 2825
Size: 6070
BamHI G'GATC_C
Cuts at: 3400 3767 3400
Size: 367 5703
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3391 4846 618
Size: 325 217 627 1604 1455 1842
Fragments arranged by size:
1842 1604 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2213 3389 1391
Size: 822 1176 4072
Fragments arranged by size:
4072 1176 822
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3210 928
Size: 971 1311 3788
Fragments arranged by size:
3788 1311 971
BbvCI CC'TCA_GC
Cuts at: 3366 3366
Size: 6070
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2646 2680 4261 4295 1903
Size: 34 709 34 1581 34 3678
Fragments arranged by size:
3678 1581 709 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3962 5172 3962
Size: 1210 4860
BclI T'GATC_A
Cuts at: 1548 3154 1548
Size: 1606 4464
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4681 136
Size: 107 122 71 631 605 3009 1525
Fragments arranged by size:
3009 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6065 6065
Size: 6070
BlpI GC'TnA_GC
Cuts at: 3487 3487
Size: 6070
Bme1580I G_kGCm'C
Cuts at: 1790 2178 4131 5287 1790
Size: 388 1953 1156 2573
Fragments arranged by size:
2573 1953 1156 388
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6070
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2065 3801 4759
Size: 416 53 83 399 24 648 1736 958
Cuts at: 4759 442
Size: 1753
Fragments arranged by size:
1753 1736 958 648 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3308 1056
Size: 2252 3818
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2913 3265 2913
Size: 352 5718
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4730 1239
Size: 3491 2579
Bpu10I CC'TnA_GC
Cuts at: 2212 3366 2212
Size: 1154 4916
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2368 4195 5060 5358 2368
Size: 1827 865 298 3080
Fragments arranged by size:
3080 1827 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4733 882
Size: 617 3234 2219
Fragments arranged by size:
3234 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3861 493
Size: 711 2657 2702
Fragments arranged by size:
2702 2657 711
BsaBI GATnn'nnATC
Cuts at: 6064 6064
Size: 6070
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2634 3877 4259 4894
Size: 53 83 186 936 1101 1243 382 635
Cuts at: 4894 275
Size: 1451
Fragments arranged by size:
1451 1243 1101 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4503 4901 5175 5322 5733 4503
Size: 398 274 147 411 4840
Fragments arranged by size:
4840 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2952 2982 1178
Size: 30 165 30 1549 30 4266
Fragments arranged by size:
4266 1549 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2660 1186
Size: 1474 4596
BseYI C'CCAG_C
Cuts at: 2832 3010 5273 5769 2832
Size: 178 2263 496 3133
Fragments arranged by size:
3133 2263 496 178
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2162 2423 1163
Size: 573 426 261 4810
Fragments arranged by size:
4810 573 426 261
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3520 4281 5309 1540
Size: 275 186 1519 761 1028 2301
Fragments arranged by size:
2301 1519 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2213 4131 4216 5287 2213
Size: 1918 85 1071 2996
Fragments arranged by size:
2996 1918 1071 85
BsmI GAATG_Cn'
Cuts at: 3581 3674 3581
Size: 93 5977
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2178 2213 3389 4131 4216 5287 1391
Size: 399 388 35 1176 742 85 1071 2174
Fragments arranged by size:
2174 1176 1071 742 399 388 85 35
BspEI T'CCGG_A
Cuts at: 5733 5733
Size: 6070
BspHI T'CATG_A
Cuts at: 2262 2637 3959 5689 2262
Size: 375 1322 1730 2643
Fragments arranged by size:
2643 1730 1322 375
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2118 3425 844
Size: 1274 1307 3489
Fragments arranged by size:
3489 1307 1274
BsrBI CCG'CTC
Cuts at: 1394 1823 3957 1394
Size: 429 2134 3507
Fragments arranged by size:
3507 2134 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4565 4739 65
Size: 1183 3317 174 1396
Fragments arranged by size:
3317 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4714 4901 1700
Size: 110 2904 187 2869
Fragments arranged by size:
2904 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6070
BssHII G'CGCG_C
Cuts at: 1925 2146 2148 1925
Size: 221 2 5847
Fragments arranged by size:
5847 221 2
BssSI C'ACGA_G
Cuts at: 4130 5142 4130
Size: 1012 5058
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2173 2410 794
Size: 1379 237 4454
Fragments arranged by size:
4454 1379 237
BstBI TT'CG_AA
Cuts at: 4874 4874
Size: 6070
BstEII G'GTnAC_C
Cuts at: 1184 3221 1184
Size: 2037 4033
BstYI r'GATC_y
Cuts at: 1972 2226 3400 3767 4152 4169 5621 5707 5719
Size: 254 1174 367 385 17 1452 86 12
Cuts at: 5719 6065 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1174 385 367 346 254 86
17 12
BstZ17I GTA'TAC
Cuts at: 2573 2573
Size: 6070
Bsu36I CC'TnA_GG
Cuts at: 2385 2385
Size: 6070
BtgI C'CryG_G
Cuts at: 513 1065 2136 3208 3351 513
Size: 552 1071 1072 143 3232
Fragments arranged by size:
3232 1072 1071 552 143
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2233 487
Size: 683 56 1007 4324
Fragments arranged by size:
4324 1007 683 56
BtsI GCAGTG_nn'
Cuts at: 3132 3586 4380 4400 3132
Size: 454 794 20 4802
Fragments arranged by size:
4802 794 454 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6070
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6035
DraI TTT'AAA
Cuts at: 3374 3374
Size: 6070
DraIII CAC_nnn'GTG
Cuts at: 2173 2173
Size: 6070
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5077 809
Size: 507 3761 1802
Fragments arranged by size:
3761 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2437 2622 3211
Size: 54 1425 50 275 18 607 185 589
Cuts at: 3211 3517 4406 6035 8
Size: 306 889 1629 43
Fragments arranged by size:
1629 1425 889 607 589 306 275 185
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3517 1537
Size: 275 1705 4090
Fragments arranged by size:
4090 1705 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2201 2792 3291 4000 1353
Size: 848 591 499 709 3423
Fragments arranged by size:
3423 848 709 591 499
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4657 5031 5177 387
Size: 1064 138 3068 374 146 1280
Fragments arranged by size:
3068 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2211 2211
Size: 6070
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2147 3315 4133 4730 5517 5785 1239
Size: 393 515 1168 818 597 787 268 1524
Fragments arranged by size:
1524 1168 818 787 597 515 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2829 3346 2829
Size: 517 5553
EcoRI G'AATT_C
Cuts at: 2023 2059 2564 3190 3379 3757 2023
Size: 36 505 626 189 378 4336
Fragments arranged by size:
4336 626 505 378 189 36
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2512 2803 2512
Size: 291 5779
FspI TGC'GCA
Cuts at: 4576 4576
Size: 6070
HaeII r_GCGC'y
Cuts at: 1678 5217 1678
Size: 3539 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2211 2243 4711 4743 4785
Size: 32 135 32 639 32 2468 32 42
Cuts at: 4785 4817 1373
Size: 32 2626
Fragments arranged by size:
2626 2468 639 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3414 3660 669
Size: 988 281 1476 246 3079
Fragments arranged by size:
3079 1476 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6070
HpaI GTT'AAC
Cuts at: 3660 3660
Size: 6070
KpnI G_GTAC'C
Cuts at: 3395 3395
Size: 6070
MfeI C'AATT_G
Cuts at: 3669 3669
Size: 6070
MluI A'CGCG_T
Cuts at: 5946 5946
Size: 6070
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2088 2863 2968 5184 5368 1305
Size: 783 775 105 2216 184 2007
Fragments arranged by size:
2216 2007 783 775 184 105
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2439 3213 10
Size: 54 1425 343 607 774 2867
Fragments arranged by size:
2867 1425 774 607 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4028 4387 4546 518
Size: 648 2862 359 159 2042
Fragments arranged by size:
2862 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3486 4163 5311 5556 1606
Size: 1880 677 1148 245 2120
Fragments arranged by size:
2120 1880 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 3208 3351 513
Size: 552 2143 143 3232
Fragments arranged by size:
3232 2143 552 143
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6070
NheI G'CTAG_C
Cuts at: 1052 3304 1052
Size: 2252 3818
NotI GC'GGCC_GC
Cuts at: 3517 3517
Size: 6070
NspI r_CATG'y
Cuts at: 1132 1229 2159 2777 3290 3428 4973 1132
Size: 97 930 618 513 138 1545 2229
Fragments arranged by size:
2229 1545 930 618 513 138 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6070
PciI A'CATG_T
Cuts at: 2773 4969 2773
Size: 2196 3874
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6070
PmeI GTTT'AAAC
Cuts at: 3374 3374
Size: 6070
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4139 4171 5652 5684 4139
Size: 32 1481 32 4525
Fragments arranged by size:
4525 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3346 3346
Size: 6070
PshAI GACnn'nnGTC
Cuts at: 1613 2121 1613
Size: 508 5562
PsiI TTA'TAA
Cuts at: 3640 3640
Size: 6070
PstI C_TGCA'G
Cuts at: 830 3422 830
Size: 2592 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6070
SacI G_AGCT'C
Cuts at: 2213 2213
Size: 6070
SalI G'TCGA_C
Cuts at: 1936 3412 1936
Size: 1476 4594
SanDI GG'GwC_CC
Cuts at: 3346 3346
Size: 6070
SapI GCTCTTCn'nnn_
Cuts at: 2201 2201
Size: 6070
SbfI CC_TGCA'GG
Cuts at: 3422 3422
Size: 6070
ScaI AGT'ACT
Cuts at: 4318 6030 4318
Size: 1712 4358
SexAI A'CCwGG_T
Cuts at: 2586 2586
Size: 6070
SfcI C'TryA_G
Cuts at: 826 1045 3418 4553 5234 5425 826
Size: 219 2373 1135 681 191 1471
Fragments arranged by size:
2373 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6070
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6070
SmaI CCC'GGG
Cuts at: 1872 3397 1872
Size: 1525 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2347 2869 4174 5075 5337 820
Size: 197 926 404 522 1305 901 262 1553
Fragments arranged by size:
1553 1305 926 901 522 404 262 197
SnaBI TAC'GTA
Cuts at: 493 3861 493
Size: 3368 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6070
SphI G_CATG'C
Cuts at: 1132 3428 1132
Size: 2296 3774
SspI AAT'ATT
Cuts at: 5 52 2278 2461 3994 5
Size: 47 2226 183 1533 2081
Fragments arranged by size:
2226 2081 1533 183 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6070
StyI C'CwwG_G
Cuts at: 513 1065 2754 2825 3208 3351 3509 513
Size: 552 1689 71 383 143 158 3074
Fragments arranged by size:
3074 1689 552 383 158 143 71
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2117 4097 4114 4267 4452 4897 325
Size: 1255 537 1980 17 153 185 445 1498
Fragments arranged by size:
1980 1498 1255 537 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2841 4316 6028
Size: 274 80 33 51 1443 864 1475 1712
Cuts at: 6028 96
Size: 138
Fragments arranged by size:
1712 1475 1443 864 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2952 2984 6030 6062 2952
Size: 32 3046 32 2960
Fragments arranged by size:
3046 2960 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6070
XbaI T'CTAG_A
Cuts at: 3406 3406
Size: 6070
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2752 3358 2752
Size: 606 5464
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6070
XmaI C'CCGG_G
Cuts at: 1870 3395 1870
Size: 1525 4545
XmnI GAAnn'nnTTC
Cuts at: 2495 4199 2495
Size: 1704 4366
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2635 3878 276
Size: 53 83 186 936 1101 1243 2468
Fragments arranged by size:
2468 1243 1101 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BaeI BbeI BfrBI BsiWI
BsmBI BstXI EcoNI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI PvuII RsrII SacII SfiI SfoI SrfI
SwaI