Restriction map for
pFN21AB8121
(Circular) (Six-base) MAPSORT of: pFN21AB8121.seq Check: 8476 from: 1 to: 5665
pFN21AB8121 5665 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3475 278
Size: 53 83 186 936 1939 2468
Fragments arranged by size:
2468 1939 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3008 1937
Size: 1071 4594
Acc65I G'GTAC_C
Cuts at: 2986 2986
Size: 5665
AclI AA'CG_TT
Cuts at: 1563 3792 4165 1563
Size: 2229 373 3063
Fragments arranged by size:
3063 2229 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2373 2653 3728 5112 5380 1632
Size: 741 280 1075 1384 268 1917
Fragments arranged by size:
1917 1384 1075 741 280 268
AfeI AGC'GCT
Cuts at: 2162 2162
Size: 5665
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5468
AflIII A'CryG_T
Cuts at: 1203 4564 5541 1203
Size: 3361 977 1327
Fragments arranged by size:
3361 1327 977
AgeI A'CCGG_T
Cuts at: 4496 4496
Size: 5665
AhdI GACnn_n'nnGTC
Cuts at: 4394 4394
Size: 5665
AleI CACnn'nnGTG
Cuts at: 1166 2526 1166
Size: 1360 4305
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5665
AlwNI CAG_nnn'CTG
Cuts at: 3408 4980 3408
Size: 1572 4093
ApaLI G'TGCA_C
Cuts at: 3722 4878 3722
Size: 1156 4509
ApoI r'AATT_y
Cuts at: 1493 2818 2842 2974 3200 3352 5461 1493
Size: 1325 24 132 226 152 2109 1697
Fragments arranged by size:
2109 1697 1325 226 152 132 24
AseI AT'TA_AT
Cuts at: 160 1032 4219 160
Size: 872 3187 1606
Fragments arranged by size:
3187 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5665
AvaI C'yCGr_G
Cuts at: 1870 1943 2990 1870
Size: 73 1047 4545
Fragments arranged by size:
4545 1047 73
BamHI G'GATC_C
Cuts at: 2995 3362 2995
Size: 367 5298
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2300 2986 4441 618
Size: 325 217 627 513 686 1455 1842
Fragments arranged by size:
1842 1455 686 627 513 325 217
BanII G_rGCy'C
Cuts at: 1391 2034 2984 1391
Size: 643 950 4072
Fragments arranged by size:
4072 950 643
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2352 2805 928
Size: 971 453 453 3788
Fragments arranged by size:
3788 971 453 453
BbvCI CC'TCA_GC
Cuts at: 2042 2042
Size: 5665
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3856 3890 1903
Size: 34 1919 34 3678
Fragments arranged by size:
3678 1919 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3557 4767 3557
Size: 1210 4455
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5665
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2871 4276 136
Size: 107 122 71 631 605 1199 1405 1525
Fragments arranged by size:
1525 1405 1199 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5660 5660
Size: 5665
BlpI GC'TnA_GC
Cuts at: 3082 3082
Size: 5665
Bme1580I G_kGCm'C
Cuts at: 1790 2305 3726 4882 1790
Size: 515 1421 1156 2573
Fragments arranged by size:
2573 1421 1156 515
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5665
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3396 4354 442
Size: 416 53 83 399 24 1979 958 1753
Fragments arranged by size:
1979 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5665
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2149 2260 4325 1239
Size: 910 111 2065 2579
Fragments arranged by size:
2579 2065 910 111
Bpu10I CC'TnA_GC
Cuts at: 2042 2042
Size: 5665
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3790 4655 4953 3790
Size: 865 298 4502
Fragments arranged by size:
4502 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4328 882
Size: 617 2829 2219
Fragments arranged by size:
2829 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3456 493
Size: 711 2252 2702
Fragments arranged by size:
2702 2252 711
BsaBI GATnn'nnATC
Cuts at: 2408 5659 2408
Size: 3251 2414
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3472 3854 4489 275
Size: 53 83 186 936 1939 382 635 1451
Fragments arranged by size:
1939 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4098 4496 4770 4917 5328 4098
Size: 398 274 147 411 4435
Fragments arranged by size:
4435 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2459 2489 1178
Size: 30 165 30 1056 30 4354
Fragments arranged by size:
4354 1056 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2110 2216 1186
Size: 924 106 4635
Fragments arranged by size:
4635 924 106
BseYI C'CCAG_C
Cuts at: 4868 5364 4868
Size: 496 5169
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5092
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2656 3115 3876 4904 1540
Size: 275 186 655 459 761 1028 2301
Fragments arranged by size:
2301 1028 761 655 459 275 186
BsiHKAI G_wGCw'C
Cuts at: 3726 3811 4882 3726
Size: 85 1071 4509
Fragments arranged by size:
4509 1071 85
BsmI GAATG_Cn'
Cuts at: 2227 3176 3269 2227
Size: 949 93 4623
Fragments arranged by size:
4623 949 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 2305 2984 3726 3811 4882 1391
Size: 399 244 271 679 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 679 399 271 244 85
BspEI T'CCGG_A
Cuts at: 5328 5328
Size: 5665
BspHI T'CATG_A
Cuts at: 2412 3554 5284 2412
Size: 1142 1730 2793
Fragments arranged by size:
2793 1730 1142
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3020 844
Size: 2176 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3552 1394
Size: 429 1729 3507
Fragments arranged by size:
3507 1729 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2117 4160 4334 65
Size: 1183 869 2043 174 1396
Fragments arranged by size:
2043 1396 1183 869 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4309 4496 1700
Size: 110 2499 187 2869
Fragments arranged by size:
2869 2499 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5665
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5665
BssSI C'ACGA_G
Cuts at: 3725 4737 3725
Size: 1012 4653
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5665
BstBI TT'CG_AA
Cuts at: 4469 4469
Size: 5665
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5665
BstYI r'GATC_y
Cuts at: 1972 2146 2995 3362 3747 3764 5216 5302 5314
Size: 174 849 367 385 17 1452 86 12
Cuts at: 5314 5660 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 849 385 367 346 174 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2093 2093
Size: 5665
BtgI C'CryG_G
Cuts at: 513 1065 2478 2698 513
Size: 552 1413 220 3480
Fragments arranged by size:
3480 1413 552 220
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4926
Fragments arranged by size:
4926 683 56
BtsI GCAGTG_nn'
Cuts at: 2434 2728 2956 3181 3975 3995 2434
Size: 294 228 225 794 20 4104
Fragments arranged by size:
4104 794 294 228 225 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5665
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5630
DraI TTT'AAA
Cuts at: 2969 2969
Size: 5665
DraIII CAC_nnn'GTG
Cuts at: 2239 2239
Size: 5665
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4672 809
Size: 507 3356 1802
Fragments arranged by size:
3356 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2518 3112 4001
Size: 54 1425 50 275 18 688 594 889
Cuts at: 4001 5630 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 688 594 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3112 1537
Size: 275 1300 4090
Fragments arranged by size:
4090 1300 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2057 2242 3595 1353
Size: 704 185 1353 3423
Fragments arranged by size:
3423 1353 704 185
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4252 4626 4772 387
Size: 1064 138 2663 374 146 1280
Fragments arranged by size:
2663 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2149 2260 2373 2653 3728 4325 5112
Size: 393 517 111 113 280 1075 597 787
Cuts at: 5112 5380 1239
Size: 268 1524
Fragments arranged by size:
1524 1075 787 597 517 393 280 268
113 111
EcoRI G'AATT_C
Cuts at: 2974 3352 2974
Size: 378 5287
FspI TGC'GCA
Cuts at: 4171 4171
Size: 5665
HaeII r_GCGC'y
Cuts at: 1678 2164 4812 1678
Size: 486 2648 2531
Fragments arranged by size:
2648 2531 486
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2459 2491 4306 4338 4380
Size: 32 135 32 887 32 1815 32 42
Cuts at: 4380 4412 1373
Size: 32 2626
Fragments arranged by size:
2626 1815 887 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3009 3255 669
Size: 988 281 1071 246 3079
Fragments arranged by size:
3079 1071 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5665
HpaI GTT'AAC
Cuts at: 3255 3255
Size: 5665
KpnI G_GTAC'C
Cuts at: 2990 2990
Size: 5665
MfeI C'AATT_G
Cuts at: 3264 3264
Size: 5665
MluI A'CGCG_T
Cuts at: 5541 5541
Size: 5665
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4779 4963 1305
Size: 3474 184 2007
Fragments arranged by size:
3474 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2520 10
Size: 54 1425 343 688 3155
Fragments arranged by size:
3155 1425 688 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2526 3623 3982 4141 518
Size: 648 1360 1097 359 159 2042
Fragments arranged by size:
2042 1360 1097 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2041 3081 3758 4906 5151 1606
Size: 435 1040 677 1148 245 2120
Fragments arranged by size:
2120 1148 1040 677 435 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5113
NdeI CA'TA_TG
Cuts at: 387 2188 387
Size: 1801 3864
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5665
NotI GC'GGCC_GC
Cuts at: 3112 3112
Size: 5665
NspI r_CATG'y
Cuts at: 1132 1229 3023 4568 1132
Size: 97 1794 1545 2229
Fragments arranged by size:
2229 1794 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5665
PciI A'CATG_T
Cuts at: 4564 4564
Size: 5665
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5665
PmeI GTTT'AAAC
Cuts at: 2969 2969
Size: 5665
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3734 3766 5247 5279 3734
Size: 32 1481 32 4120
Fragments arranged by size:
4120 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5665
PsiI TTA'TAA
Cuts at: 3235 3235
Size: 5665
PstI C_TGCA'G
Cuts at: 830 3017 830
Size: 2187 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5665
PvuII CAG'CTG
Cuts at: 2041 2041
Size: 5665
SalI G'TCGA_C
Cuts at: 1936 3007 1936
Size: 1071 4594
SapI GCTCTTCn'nnn_
Cuts at: 2057 2057
Size: 5665
SbfI CC_TGCA'GG
Cuts at: 3017 3017
Size: 5665
ScaI AGT'ACT
Cuts at: 3913 5625 3913
Size: 1712 3953
SexAI A'CCwGG_T
Cuts at: 2296 2296
Size: 5665
SfcI C'TryA_G
Cuts at: 826 1045 3013 4148 4829 5020 826
Size: 219 1968 1135 681 191 1471
Fragments arranged by size:
1968 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5665
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5665
SmaI CCC'GGG
Cuts at: 1872 2992 1872
Size: 1120 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3769 4670 4932 820
Size: 197 926 1826 901 262 1553
Fragments arranged by size:
1826 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3456 493
Size: 2963 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5665
SphI G_CATG'C
Cuts at: 1132 3023 1132
Size: 1891 3774
SspI AAT'ATT
Cuts at: 5 52 3589 5
Size: 47 3537 2081
Fragments arranged by size:
3537 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5665
StyI C'CwwG_G
Cuts at: 513 1065 2908 3104 513
Size: 552 1843 196 3074
Fragments arranged by size:
3074 1843 552 196
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2436 2642 3692 3709 3862 4047 4492
Size: 1255 856 206 1050 17 153 185 445
Cuts at: 4492 325
Size: 1498
Fragments arranged by size:
1498 1255 1050 856 445 206 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3911 5623 96
Size: 274 80 33 51 1443 1934 1712 138
Fragments arranged by size:
1934 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2238 2270 5625 5657 2238
Size: 32 3355 32 2246
Fragments arranged by size:
3355 2246 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5665
XbaI T'CTAG_A
Cuts at: 3001 3001
Size: 5665
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2513 2915 2513
Size: 402 5263
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5665
XmaI C'CCGG_G
Cuts at: 1870 2990 1870
Size: 1120 4545
XmnI GAAnn'nnTTC
Cuts at: 2051 2596 3794 2051
Size: 545 1198 3922
Fragments arranged by size:
3922 1198 545
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3473 276
Size: 53 83 186 936 1939 2468
Fragments arranged by size:
2468 1939 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI
SwaI