Restriction map for
pFN21AB8119
(Circular) (Six-base) MAPSORT of: pFN21AB8119.seq Check: 4411 from: 1 to: 5365
pFN21AB8119 5365 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2268 3175 278
Size: 53 83 186 936 732 907 2468
Fragments arranged by size:
2468 936 907 732 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2060 2708 1937
Size: 123 648 4594
Fragments arranged by size:
4594 648 123
Acc65I G'GTAC_C
Cuts at: 2686 2686
Size: 5365
AclI AA'CG_TT
Cuts at: 1563 3492 3865 1563
Size: 1929 373 3063
Fragments arranged by size:
3063 1929 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3428 4812 5080 1632
Size: 1796 1384 268 1917
Fragments arranged by size:
1917 1796 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5168
AflIII A'CryG_T
Cuts at: 1203 4264 5241 1203
Size: 3061 977 1327
Fragments arranged by size:
3061 1327 977
AgeI A'CCGG_T
Cuts at: 4196 4196
Size: 5365
AhdI GACnn_n'nnGTC
Cuts at: 4094 4094
Size: 5365
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5365
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2324 1184
Size: 1140 4225
AlwNI CAG_nnn'CTG
Cuts at: 2468 3108 4680 2468
Size: 640 1572 3153
Fragments arranged by size:
3153 1572 640
ApaI G_GGCC'C
Cuts at: 2289 2289
Size: 5365
ApaLI G'TGCA_C
Cuts at: 3422 4578 3422
Size: 1156 4209
ApoI r'AATT_y
Cuts at: 1493 2674 2900 3052 5161 1493
Size: 1181 226 152 2109 1697
Fragments arranged by size:
2109 1697 1181 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3919 160
Size: 872 2887 1606
Fragments arranged by size:
2887 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5365
AvaI C'yCGr_G
Cuts at: 1870 1943 2290 2690 1870
Size: 73 347 400 4545
Fragments arranged by size:
4545 400 347 73
BamHI G'GATC_C
Cuts at: 2695 3062 2695
Size: 367 4998
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2686 4141 618
Size: 325 217 627 899 1455 1842
Fragments arranged by size:
1842 1455 899 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2289 2330 2684 1391
Size: 898 41 354 4072
Fragments arranged by size:
4072 898 354 41
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4394
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2493 2527 3556 3590 1903
Size: 34 556 34 1029 34 3678
Fragments arranged by size:
3678 1029 556 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3257 4467 3257
Size: 1210 4155
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5365
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2054 3976 136
Size: 107 122 71 631 605 382 1922 1525
Fragments arranged by size:
1922 1525 631 605 382 122 107 71
BglII A'GATC_T
Cuts at: 5360 5360
Size: 5365
BlpI GC'TnA_GC
Cuts at: 2276 2782 2276
Size: 506 4859
Bme1580I G_kGCm'C
Cuts at: 1790 2289 2313 3426 4582 1790
Size: 499 24 1113 1156 2573
Fragments arranged by size:
2573 1156 1113 499 24
BmgBI CAC'GTC
Cuts at: 1318 2197 1318
Size: 879 4486
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2131 2215 3096
Size: 416 53 83 399 24 714 84 881
Cuts at: 3096 4054 442
Size: 958 1753
Fragments arranged by size:
1753 958 881 714 416 399 84 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5365
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2255 4025 1239
Size: 1016 1770 2579
Fragments arranged by size:
2579 1770 1016
Bpu10I CC'TnA_GC
Cuts at: 2324 2324
Size: 5365
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3490 4355 4653 3490
Size: 865 298 4202
Fragments arranged by size:
4202 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4028 882
Size: 617 2529 2219
Fragments arranged by size:
2529 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3156 493
Size: 711 1952 2702
Fragments arranged by size:
2702 1952 711
BsaBI GATnn'nnATC
Cuts at: 5359 5359
Size: 5365
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2057 2265 3172 3554
Size: 53 83 186 936 524 208 907 382
Cuts at: 3554 4189 275
Size: 635 1451
Fragments arranged by size:
1451 936 907 635 524 382 208 186
83 53
BsaWI w'CCGG_w
Cuts at: 3798 4196 4470 4617 5028 3798
Size: 398 274 147 411 4135
Fragments arranged by size:
4135 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2117 2147 1178
Size: 30 165 30 714 30 4396
Fragments arranged by size:
4396 714 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5365
BseYI C'CCAG_C
Cuts at: 4568 5064 4568
Size: 496 4869
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4792
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2107 2251 2815 3576 4604 1540
Size: 275 186 106 144 564 761 1028 2301
Fragments arranged by size:
2301 1028 761 564 275 186 144 106
BsiHKAI G_wGCw'C
Cuts at: 3426 3511 4582 3426
Size: 85 1071 4209
Fragments arranged by size:
4209 1071 85
BsmI GAATG_Cn'
Cuts at: 2509 2876 2969 2509
Size: 367 93 4905
Fragments arranged by size:
4905 367 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2203 2203
Size: 5365
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2289 2313 2330 2684 3426 3511 4582
Size: 399 499 24 17 354 742 85 1071
Cuts at: 4582 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 499 399 354 85 24
17
BspEI T'CCGG_A
Cuts at: 5028 5028
Size: 5365
BspHI T'CATG_A
Cuts at: 3254 4984 3254
Size: 1730 3635
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2361 2720 844
Size: 1517 359 3489
Fragments arranged by size:
3489 1517 359
BsrBI CCG'CTC
Cuts at: 1394 1823 3252 1394
Size: 429 1429 3507
Fragments arranged by size:
3507 1429 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3860 4034 65
Size: 1183 2612 174 1396
Fragments arranged by size:
2612 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2250 4009 4196 1700
Size: 110 440 1759 187 2869
Fragments arranged by size:
2869 1759 440 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5365
BssHII G'CGCG_C
Cuts at: 1925 2030 2032 2171 1925
Size: 105 2 139 5119
Fragments arranged by size:
5119 139 105 2
BssSI C'ACGA_G
Cuts at: 2078 3425 4437 2078
Size: 1347 1012 3006
Fragments arranged by size:
3006 1347 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5365
BstBI TT'CG_AA
Cuts at: 4169 4169
Size: 5365
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5365
BstXI CCAn_nnnn'nTGG
Cuts at: 2389 2389
Size: 5365
BstYI r'GATC_y
Cuts at: 1972 2695 3062 3447 3464 4916 5002 5014 5360
Size: 723 367 385 17 1452 86 12 346
Cuts at: 5360 1972
Size: 1977
Fragments arranged by size:
1977 1452 723 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2020 2113 513
Size: 552 939 16 93 3765
Fragments arranged by size:
3765 939 552 93 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4626
Fragments arranged by size:
4626 683 56
BtsI GCAGTG_nn'
Cuts at: 2036 2881 3675 3695 2036
Size: 845 794 20 3706
Fragments arranged by size:
3706 845 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5365
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5330
DraI TTT'AAA
Cuts at: 2669 2669
Size: 5365
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4372 809
Size: 507 3056 1802
Fragments arranged by size:
3056 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2104 2217
Size: 54 1425 50 275 18 177 97 113
Cuts at: 2217 2248 2812 3701 5330 8
Size: 31 564 889 1629 43
Fragments arranged by size:
1629 1425 889 564 275 177 113 97
54 50 43 31 18
EagI C'GGCC_G
Cuts at: 1537 1812 2104 2248 2812 1537
Size: 275 292 144 564 4090
Fragments arranged by size:
4090 564 292 275 144
EarI CTCTTCn'nnn_
Cuts at: 1353 3295 1353
Size: 1942 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3952 4326 4472 387
Size: 1064 138 2363 374 146 1280
Fragments arranged by size:
2363 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2255 3428 4025 4812 5080 1239
Size: 393 623 1173 597 787 268 1524
Fragments arranged by size:
1524 1173 787 623 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2286 2599 2286
Size: 313 5052
EcoRI G'AATT_C
Cuts at: 2674 3052 2674
Size: 378 4987
FseI GG_CCGG'CC
Cuts at: 2254 2254
Size: 5365
FspI TGC'GCA
Cuts at: 3871 3871
Size: 5365
HaeII r_GCGC'y
Cuts at: 1678 2075 2214 4512 1678
Size: 397 139 2298 2531
Fragments arranged by size:
2531 2298 397 139
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2117 2149 2255 2287 2610
Size: 32 135 32 545 32 106 32 323
Cuts at: 2610 2642 4006 4038 4080 4112 1373
Size: 32 1364 32 42 32 2626
Fragments arranged by size:
2626 1364 545 323 135 106 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2061 2709 2955 669
Size: 988 281 123 648 246 3079
Fragments arranged by size:
3079 988 648 281 246 123
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5365
HpaI GTT'AAC
Cuts at: 2955 2955
Size: 5365
KpnI G_GTAC'C
Cuts at: 2690 2690
Size: 5365
MfeI C'AATT_G
Cuts at: 2964 2964
Size: 5365
MluI A'CGCG_T
Cuts at: 5241 5241
Size: 5365
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2226 4479 4663 1305
Size: 921 2253 184 2007
Fragments arranged by size:
2253 2007 921 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2219 10
Size: 54 1425 343 387 3156
Fragments arranged by size:
3156 1425 387 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3323 3682 3841 518
Size: 648 2157 359 159 2042
Fragments arranged by size:
2157 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2022 2304 2332 2781 3458 4606 4851 1606
Size: 416 282 28 449 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 449 416 282 245 28
NaeI GCC'GGC
Cuts at: 2252 2252
Size: 5365
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3874
Fragments arranged by size:
3874 939 552
NdeI CA'TA_TG
Cuts at: 387 2368 387
Size: 1981 3384
NgoMIV G'CCGG_C
Cuts at: 2250 2250
Size: 5365
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5365
NotI GC'GGCC_GC
Cuts at: 2812 2812
Size: 5365
NspI r_CATG'y
Cuts at: 1132 1229 2723 4268 1132
Size: 97 1494 1545 2229
Fragments arranged by size:
2229 1545 1494 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5365
PciI A'CATG_T
Cuts at: 4264 4264
Size: 5365
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2595 1108
Size: 1487 3878
PfoI T'CCnGG_A
Cuts at: 2601 2601
Size: 5365
PmeI GTTT'AAAC
Cuts at: 2669 2669
Size: 5365
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3434 3466 4947 4979 3434
Size: 32 1481 32 3820
Fragments arranged by size:
3820 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2599 2599
Size: 5365
PshAI GACnn'nnGTC
Cuts at: 1613 2195 1613
Size: 582 4783
PsiI TTA'TAA
Cuts at: 2649 2935 2649
Size: 286 5079
PspOMI G'GGCC_C
Cuts at: 2285 2285
Size: 5365
PstI C_TGCA'G
Cuts at: 830 2337 2358 2717 830
Size: 1507 21 359 3478
Fragments arranged by size:
3478 1507 359 21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5365
SacII CC_GC'GG
Cuts at: 2023 2023
Size: 5365
SalI G'TCGA_C
Cuts at: 1936 2059 2707 1936
Size: 123 648 4594
Fragments arranged by size:
4594 648 123
SbfI CC_TGCA'GG
Cuts at: 2358 2717 2358
Size: 359 5006
ScaI AGT'ACT
Cuts at: 3613 5325 3613
Size: 1712 3653
SfcI C'TryA_G
Cuts at: 826 1045 2333 2354 2713 3848 4529 4720 826
Size: 219 1288 21 359 1135 681 191 1471
Fragments arranged by size:
1471 1288 1135 681 359 219 191 21
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5365
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5365
SmaI CCC'GGG
Cuts at: 1872 2292 2692 1872
Size: 420 400 4545
Fragments arranged by size:
4545 420 400
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3469 4370 4632 820
Size: 197 926 1526 901 262 1553
Fragments arranged by size:
1553 1526 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3156 493
Size: 2663 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5365
SphI G_CATG'C
Cuts at: 1132 2723 1132
Size: 1591 3774
SspI AAT'ATT
Cuts at: 5 52 2645 3289 5
Size: 47 2593 644 2081
Fragments arranged by size:
2593 2081 644 47
StuI AGG'CCT
Cuts at: 1517 2132 1517
Size: 615 4750
StyI C'CwwG_G
Cuts at: 513 1065 2004 2475 2804 513
Size: 552 939 471 329 3074
Fragments arranged by size:
3074 939 552 471 329
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3392 3409 3562 3747 4192 325
Size: 1255 1812 17 153 185 445 1498
Fragments arranged by size:
1812 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3611 5323 96
Size: 274 80 33 51 1443 1634 1712 138
Fragments arranged by size:
1712 1634 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5325 5357 5325
Size: 32 5333
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5365
XbaI T'CTAG_A
Cuts at: 2701 2701
Size: 5365
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2230 2230
Size: 5365
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5365
XmaI C'CCGG_G
Cuts at: 1870 2290 2690 1870
Size: 420 400 4545
Fragments arranged by size:
4545 420 400
XmnI GAAnn'nnTTC
Cuts at: 3494 3494
Size: 5365
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2266 3173 276
Size: 53 83 186 936 732 907 2468
Fragments arranged by size:
2468 936 907 732 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FseI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FspAI KasI NarI NruI NsiI PacI
PmlI PspXI PsrI PvuII RsrII SacI SanDI SapI
SexAI SfiI SfoI SrfI SwaI