Restriction map for
pFN21AB8116
(Circular) (Six-base) MAPSORT of: pFN21AB8116.seq Check: 485 from: 1 to: 5464
pFN21AB8116 5464 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3274 278
Size: 53 83 186 936 1738 2468
Fragments arranged by size:
2468 1738 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2132 2807 1937
Size: 195 675 4594
Fragments arranged by size:
4594 675 195
Acc65I G'GTAC_C
Cuts at: 2785 2785
Size: 5464
AclI AA'CG_TT
Cuts at: 1563 3591 3964 1563
Size: 2028 373 3063
Fragments arranged by size:
3063 2028 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2616 3527 4911 5179 1632
Size: 984 911 1384 268 1917
Fragments arranged by size:
1917 1384 984 911 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5267
AflIII A'CryG_T
Cuts at: 1203 4363 5340 1203
Size: 3160 977 1327
Fragments arranged by size:
3160 1327 977
AgeI A'CCGG_T
Cuts at: 4295 4295
Size: 5464
AhdI GACnn_n'nnGTC
Cuts at: 4193 4193
Size: 5464
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5464
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2123 1184
Size: 939 4525
AlwNI CAG_nnn'CTG
Cuts at: 2539 2654 3207 4779 2539
Size: 115 553 1572 3224
Fragments arranged by size:
3224 1572 553 115
ApaI G_GGCC'C
Cuts at: 2553 2553
Size: 5464
ApaLI G'TGCA_C
Cuts at: 3521 4677 3521
Size: 1156 4308
ApoI r'AATT_y
Cuts at: 1493 2773 2999 3151 5260 1493
Size: 1280 226 152 2109 1697
Fragments arranged by size:
2109 1697 1280 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4018 160
Size: 872 2986 1606
Fragments arranged by size:
2986 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5464
AvaI C'yCGr_G
Cuts at: 1870 1943 2789 1870
Size: 73 846 4545
Fragments arranged by size:
4545 846 73
BamHI G'GATC_C
Cuts at: 2794 3161 2794
Size: 367 5097
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2067 2785 4240 618
Size: 325 217 627 280 718 1455 1842
Fragments arranged by size:
1842 1455 718 627 325 280 217
BanII G_rGCy'C
Cuts at: 1391 2128 2553 2783 1391
Size: 737 425 230 4072
Fragments arranged by size:
4072 737 425 230
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2656 928
Size: 971 757 3736
Fragments arranged by size:
3736 971 757
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3655 3689 1903
Size: 34 50 34 1634 34 3678
Fragments arranged by size:
3678 1634 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3356 4566 3356
Size: 1210 4254
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5464
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4075 136
Size: 107 122 71 631 605 2403 1525
Fragments arranged by size:
2403 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5459 5459
Size: 5464
BlpI GC'TnA_GC
Cuts at: 2881 2881
Size: 5464
Bme1580I G_kGCm'C
Cuts at: 1790 2553 3525 4681 1790
Size: 763 972 1156 2573
Fragments arranged by size:
2573 1156 972 763
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5464
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2340 3195 4153
Size: 416 53 83 399 24 923 855 958
Cuts at: 4153 442
Size: 1753
Fragments arranged by size:
1753 958 923 855 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5464
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2216 2705 2726 4124 1239
Size: 977 489 21 1398 2579
Fragments arranged by size:
2579 1398 977 489 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3589 4454 4752 3589
Size: 865 298 4301
Fragments arranged by size:
4301 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4127 882
Size: 617 2628 2219
Fragments arranged by size:
2628 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3255 493
Size: 711 2051 2702
Fragments arranged by size:
2702 2051 711
BsaBI GATnn'nnATC
Cuts at: 5458 5458
Size: 5464
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3271 3653 4288 275
Size: 53 83 186 936 1738 382 635 1451
Fragments arranged by size:
1738 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3897 4295 4569 4716 5127 3897
Size: 398 274 147 411 4234
Fragments arranged by size:
4234 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5239
Fragments arranged by size:
5239 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2363 1186
Size: 1177 4287
BseYI C'CCAG_C
Cuts at: 2162 2715 4667 5163 2162
Size: 553 1952 496 2463
Fragments arranged by size:
2463 1952 553 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2555 1163
Size: 573 819 4072
Fragments arranged by size:
4072 819 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2914 3675 4703 1540
Size: 275 186 913 761 1028 2301
Fragments arranged by size:
2301 1028 913 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2490 3525 3610 4681 2490
Size: 1035 85 1071 3273
Fragments arranged by size:
3273 1071 1035 85
BsmI GAATG_Cn'
Cuts at: 2975 3068 2975
Size: 93 5371
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2128 2490 2553 2783 3525 3610 4681
Size: 399 338 362 63 230 742 85 1071
Cuts at: 4681 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 362 338 230 85
63
BspEI T'CCGG_A
Cuts at: 5127 5127
Size: 5464
BspHI T'CATG_A
Cuts at: 3353 5083 3353
Size: 1730 3734
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2244 2819 844
Size: 1400 575 3489
Fragments arranged by size:
3489 1400 575
BsrBI CCG'CTC
Cuts at: 1394 1823 3351 1394
Size: 429 1528 3507
Fragments arranged by size:
3507 1528 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2509 3959 4133 65
Size: 1183 1261 1450 174 1396
Fragments arranged by size:
1450 1396 1261 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4108 4295 1700
Size: 110 2298 187 2869
Fragments arranged by size:
2869 2298 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5464
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5464
BssSI C'ACGA_G
Cuts at: 3524 4536 3524
Size: 1012 4452
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5464
BstBI TT'CG_AA
Cuts at: 4268 4268
Size: 5464
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5464
BstYI r'GATC_y
Cuts at: 1972 2428 2794 3161 3546 3563 5015 5101 5113
Size: 456 366 367 385 17 1452 86 12
Cuts at: 5113 5459 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 456 385 367 366 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2358 2358
Size: 5464
BtgI C'CryG_G
Cuts at: 513 1065 2119 513
Size: 552 1054 3858
Fragments arranged by size:
3858 1054 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4725
Fragments arranged by size:
4725 683 56
BtsI GCAGTG_nn'
Cuts at: 2259 2385 2980 3774 3794 2259
Size: 126 595 794 20 3929
Fragments arranged by size:
3929 794 595 126 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5464
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5429
DraI TTT'AAA
Cuts at: 2768 2768
Size: 5464
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4471 809
Size: 507 3155 1802
Fragments arranged by size:
3155 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2116 2543 2911
Size: 54 1425 50 275 18 286 427 368
Cuts at: 2911 3800 5429 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 427 368 286 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2911 1537
Size: 275 1099 4090
Fragments arranged by size:
4090 1099 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2703 2760 3394 1353
Size: 1350 57 634 3423
Fragments arranged by size:
3423 1350 634 57
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4051 4425 4571 387
Size: 1064 138 2462 374 146 1280
Fragments arranged by size:
2462 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2216 2616 2705 2726 3527 4124 4911
Size: 393 584 400 89 21 801 597 787
Cuts at: 4911 5179 1239
Size: 268 1524
Fragments arranged by size:
1524 801 787 597 584 400 393 268
89 21
EcoO109I rG'GnC_Cy
Cuts at: 2362 2519 2549 2550 2362
Size: 157 30 1 5276
Fragments arranged by size:
5276 157 30 1
EcoRI G'AATT_C
Cuts at: 2773 3151 2773
Size: 378 5086
FspI TGC'GCA
Cuts at: 3970 3970
Size: 5464
HaeII r_GCGC'y
Cuts at: 1678 4611 1678
Size: 2933 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2425 2457 4105 4137 4179
Size: 32 135 32 853 32 1648 32 42
Cuts at: 4179 4211 1373
Size: 32 2626
Fragments arranged by size:
2626 1648 853 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2808 3054 669
Size: 988 281 870 246 3079
Fragments arranged by size:
3079 988 870 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5464
HpaI GTT'AAC
Cuts at: 3054 3054
Size: 5464
KpnI G_GTAC'C
Cuts at: 2789 2789
Size: 5464
MfeI C'AATT_G
Cuts at: 3063 3063
Size: 5464
MluI A'CGCG_T
Cuts at: 5340 5340
Size: 5464
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2133 4578 4762 1305
Size: 828 2445 184 2007
Fragments arranged by size:
2445 2007 828 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2118 2545 10
Size: 54 1425 343 286 427 2929
Fragments arranged by size:
2929 1425 427 343 286 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3422 3781 3940 518
Size: 648 2256 359 159 2042
Fragments arranged by size:
2256 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2452 2536 2880 3557 4705 4950 1606
Size: 846 84 344 677 1148 245 2120
Fragments arranged by size:
2120 1148 846 677 344 245 84
NcoI C'CATG_G
Cuts at: 513 1065 2119 513
Size: 552 1054 3858
Fragments arranged by size:
3858 1054 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5464
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5464
NotI GC'GGCC_GC
Cuts at: 2911 2911
Size: 5464
NspI r_CATG'y
Cuts at: 1132 1229 2211 2822 4367 1132
Size: 97 982 611 1545 2229
Fragments arranged by size:
2229 1545 982 611 97
PasI CC'CwG_GG
Cuts at: 1348 2042 1348
Size: 694 4770
PciI A'CATG_T
Cuts at: 4363 4363
Size: 5464
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2614 1108
Size: 1506 3958
PfoI T'CCnGG_A
Cuts at: 2721 2721
Size: 5464
PmeI GTTT'AAAC
Cuts at: 2768 2768
Size: 5464
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3533 3565 5046 5078 3533
Size: 32 1481 32 3919
Fragments arranged by size:
3919 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2519 2519
Size: 5464
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5464
PsiI TTA'TAA
Cuts at: 3034 3034
Size: 5464
PspOMI G'GGCC_C
Cuts at: 2549 2549
Size: 5464
PstI C_TGCA'G
Cuts at: 830 2090 2325 2673 2816 830
Size: 1260 235 348 143 3478
Fragments arranged by size:
3478 1260 348 235 143
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5464
PvuII CAG'CTG
Cuts at: 2452 2452
Size: 5464
SalI G'TCGA_C
Cuts at: 1936 2806 1936
Size: 870 4594
SapI GCTCTTCn'nnn_
Cuts at: 2760 2760
Size: 5464
SbfI CC_TGCA'GG
Cuts at: 2816 2816
Size: 5464
ScaI AGT'ACT
Cuts at: 3712 5424 3712
Size: 1712 3752
SfcI C'TryA_G
Cuts at: 826 1045 2086 2321 2669 2812 3947 4628 4819
Size: 219 1041 235 348 143 1135 681 191
Cuts at: 4819 826
Size: 1471
Fragments arranged by size:
1471 1135 1041 681 348 235 219 191
143
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5464
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5464
SmaI CCC'GGG
Cuts at: 1872 2791 1872
Size: 919 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3568 4469 4731 820
Size: 197 926 1625 901 262 1553
Fragments arranged by size:
1625 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3255 493
Size: 2762 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5464
SphI G_CATG'C
Cuts at: 1132 2211 2822 1132
Size: 1079 611 3774
Fragments arranged by size:
3774 1079 611
SspI AAT'ATT
Cuts at: 5 52 3388 5
Size: 47 3336 2081
Fragments arranged by size:
3336 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5464
StyI C'CwwG_G
Cuts at: 513 1065 2119 2903 513
Size: 552 1054 784 3074
Fragments arranged by size:
3074 1054 784 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3491 3508 3661 3846 4291 325
Size: 1255 1911 17 153 185 445 1498
Fragments arranged by size:
1911 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3710 5422 96
Size: 274 80 33 51 1443 1733 1712 138
Fragments arranged by size:
1733 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5424 5456 5424
Size: 32 5432
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5464
XbaI T'CTAG_A
Cuts at: 2800 2800
Size: 5464
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5464
XmaI C'CCGG_G
Cuts at: 1870 2789 1870
Size: 919 4545
XmnI GAAnn'nnTTC
Cuts at: 3593 3593
Size: 5464
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3272 276
Size: 53 83 186 936 1738 2468
Fragments arranged by size:
2468 1738 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI