Restriction map for
pFN21AB8115
(Circular) (Six-base) MAPSORT of: pFN21AB8115.seq Check: 2027 from: 1 to: 5905
pFN21AB8115 5905 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3715 278
Size: 53 83 186 936 2179 2468
Fragments arranged by size:
2468 2179 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3248 1937
Size: 1311 4594
Acc65I G'GTAC_C
Cuts at: 3226 3226
Size: 5905
AclI AA'CG_TT
Cuts at: 1563 4032 4405 1563
Size: 2469 373 3063
Fragments arranged by size:
3063 2469 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2129 2177 2507 3968 5352 5620 1632
Size: 497 48 330 1461 1384 268 1917
Fragments arranged by size:
1917 1461 1384 497 330 268 48
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5708
AflIII A'CryG_T
Cuts at: 1203 4804 5781 1203
Size: 3601 977 1327
Fragments arranged by size:
3601 1327 977
AgeI A'CCGG_T
Cuts at: 4736 4736
Size: 5905
AhdI GACnn_n'nnGTC
Cuts at: 4634 4634
Size: 5905
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5905
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5905
AlwNI CAG_nnn'CTG
Cuts at: 3648 5220 3648
Size: 1572 4333
ApaLI G'TGCA_C
Cuts at: 3962 5118 3962
Size: 1156 4749
ApoI r'AATT_y
Cuts at: 1493 2017 2327 2407 2657 2923 2984 3030 3214
Size: 524 310 80 250 266 61 46 184
Cuts at: 3214 3440 3592 5701 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 524 310 266 250 226 184
152 80 61 46
AseI AT'TA_AT
Cuts at: 160 1032 4459 160
Size: 872 3427 1606
Fragments arranged by size:
3427 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5905
AvaI C'yCGr_G
Cuts at: 1870 1943 3230 1870
Size: 73 1287 4545
Fragments arranged by size:
4545 1287 73
AvrII C'CTAG_G
Cuts at: 2584 2584
Size: 5905
BamHI G'GATC_C
Cuts at: 3235 3602 3235
Size: 367 5538
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3226 4681 618
Size: 325 217 627 1439 1455 1842
Fragments arranged by size:
1842 1455 1439 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2183 2817 3224 1391
Size: 792 634 407 4072
Fragments arranged by size:
4072 792 634 407
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2135 3078 928
Size: 971 236 943 3755
Fragments arranged by size:
3755 971 943 236
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4096 4130 1903
Size: 34 2159 34 3678
Fragments arranged by size:
3678 2159 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3797 5007 3797
Size: 1210 4695
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5905
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4516 136
Size: 107 122 71 631 605 2844 1525
Fragments arranged by size:
2844 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2749 5900 2749
Size: 3151 2754
BlpI GC'TnA_GC
Cuts at: 2601 3322 2601
Size: 721 5184
Bme1580I G_kGCm'C
Cuts at: 1790 3966 5122 1790
Size: 2176 1156 2573
Fragments arranged by size:
2573 2176 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5905
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3636 4594 442
Size: 416 53 83 399 24 2219 958 1753
Fragments arranged by size:
2219 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2891 1056
Size: 1835 4070
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4565 1239
Size: 3326 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2387 2683 4030 4895 5193 2387
Size: 296 1347 865 298 3099
Fragments arranged by size:
3099 1347 865 298 296
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4568 882
Size: 617 3069 2219
Fragments arranged by size:
3069 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3696 493
Size: 711 2492 2702
Fragments arranged by size:
2702 2492 711
BsaBI GATnn'nnATC
Cuts at: 5899 5899
Size: 5905
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3712 4094 4729 275
Size: 53 83 186 936 2179 382 635 1451
Fragments arranged by size:
2179 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2114 4338 4736 5010 5157 5568 2114
Size: 2224 398 274 147 411 2451
Fragments arranged by size:
2451 2224 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2736 2766 1178
Size: 30 165 30 1333 30 4317
Fragments arranged by size:
4317 1333 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2073 2825 1186
Size: 887 752 4266
Fragments arranged by size:
4266 887 752
BseYI C'CCAG_C
Cuts at: 2998 5108 5604 2998
Size: 2110 496 3299
Fragments arranged by size:
3299 2110 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5332
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3355 4116 5144 1540
Size: 275 186 1354 761 1028 2301
Fragments arranged by size:
2301 1354 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2817 3966 4051 5122 2817
Size: 1149 85 1071 3600
Fragments arranged by size:
3600 1149 1071 85
BsmI GAATG_Cn'
Cuts at: 3416 3509 3416
Size: 93 5812
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2183 2817 3224 3966 4051 5122 1391
Size: 399 393 634 407 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 634 407 399 393 85
BspEI T'CCGG_A
Cuts at: 2114 5568 2114
Size: 3454 2451
BspHI T'CATG_A
Cuts at: 2272 3794 5524 2272
Size: 1522 1730 2653
Fragments arranged by size:
2653 1730 1522
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3056 3260 844
Size: 2212 204 3489
Fragments arranged by size:
3489 2212 204
BsrBI CCG'CTC
Cuts at: 1394 1823 3792 1394
Size: 429 1969 3507
Fragments arranged by size:
3507 1969 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2229 2837 4400 4574 65
Size: 1183 981 608 1563 174 1396
Fragments arranged by size:
1563 1396 1183 981 608 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4549 4736 1700
Size: 110 2739 187 2869
Fragments arranged by size:
2869 2739 187 110
BsrGI T'GTAC_A
Cuts at: 96 2461 2863 96
Size: 2365 402 3138
Fragments arranged by size:
3138 2365 402
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5905
BssSI C'ACGA_G
Cuts at: 3965 4977 3965
Size: 1012 4893
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3180 794
Size: 2386 3519
BstBI TT'CG_AA
Cuts at: 4709 4709
Size: 5905
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5905
BstYI r'GATC_y
Cuts at: 1972 2749 3037 3235 3602 3987 4004 5456 5542
Size: 777 288 198 367 385 17 1452 86
Cuts at: 5542 5554 5900 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 777 385 367 346 288 198
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2153 513
Size: 552 1088 4265
Fragments arranged by size:
4265 1088 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5166
Fragments arranged by size:
5166 683 56
BtsI GCAGTG_nn'
Cuts at: 3178 3421 4215 4235 3178
Size: 243 794 20 4848
Fragments arranged by size:
4848 794 243 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5905
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3085 3120 529
Size: 35 2521 35 3314
Fragments arranged by size:
3314 2521 35 35
DraI TTT'AAA
Cuts at: 3209 3209
Size: 5905
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4912 809
Size: 507 3596 1802
Fragments arranged by size:
3596 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3352 4241 5870
Size: 54 1425 50 275 18 1522 889 1629
Cuts at: 5870 8
Size: 43
Fragments arranged by size:
1629 1522 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3352 1537
Size: 275 1540 4090
Fragments arranged by size:
4090 1540 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2833 3835 1353
Size: 1480 1002 3423
Fragments arranged by size:
3423 1480 1002
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4492 4866 5012 387
Size: 1064 138 2903 374 146 1280
Fragments arranged by size:
2903 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2815 2815
Size: 5905
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2129 2177 2507 3968 4565 5352 5620
Size: 393 497 48 330 1461 597 787 268
Cuts at: 5620 1239
Size: 1524
Fragments arranged by size:
1524 1461 787 597 497 393 330 268
48
EcoRI G'AATT_C
Cuts at: 2327 2407 2984 3214 3592 2327
Size: 80 577 230 378 4640
Fragments arranged by size:
4640 577 378 230 80
EcoRV GAT'ATC
Cuts at: 2278 2278
Size: 5905
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2459 2724 2459
Size: 265 5640
FspI TGC'GCA
Cuts at: 4411 4411
Size: 5905
HaeII r_GCGC'y
Cuts at: 1678 5052 1678
Size: 3374 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4546 4578 4620 4652 1373
Size: 32 135 32 2974 32 42 32 2626
Fragments arranged by size:
2974 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2691 3249 3495 669
Size: 988 281 753 558 246 3079
Fragments arranged by size:
3079 988 753 558 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5905
HpaI GTT'AAC
Cuts at: 2691 3495 2691
Size: 804 5101
KpnI G_GTAC'C
Cuts at: 3230 3230
Size: 5905
MfeI C'AATT_G
Cuts at: 3166 3504 3166
Size: 338 5567
MluI A'CGCG_T
Cuts at: 5781 5781
Size: 5905
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5019 5203 1305
Size: 3714 184 2007
Fragments arranged by size:
3714 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4083
Fragments arranged by size:
4083 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3863 4222 4381 518
Size: 648 2697 359 159 2042
Fragments arranged by size:
2697 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3002 3321 3998 5146 5391 1606
Size: 1396 319 677 1148 245 2120
Fragments arranged by size:
2120 1396 1148 677 319 245
NcoI C'CATG_G
Cuts at: 513 1065 2153 513
Size: 552 1088 4265
Fragments arranged by size:
4265 1088 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5905
NheI G'CTAG_C
Cuts at: 1052 2887 1052
Size: 1835 4070
NotI GC'GGCC_GC
Cuts at: 3352 3352
Size: 5905
NspI r_CATG'y
Cuts at: 1132 1229 3263 4808 1132
Size: 97 2034 1545 2229
Fragments arranged by size:
2229 2034 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5905
PciI A'CATG_T
Cuts at: 4804 4804
Size: 5905
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5905
PmeI GTTT'AAAC
Cuts at: 3209 3209
Size: 5905
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3974 4006 5487 5519 3974
Size: 32 1481 32 4360
Fragments arranged by size:
4360 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5905
PsiI TTA'TAA
Cuts at: 3475 3475
Size: 5905
PstI C_TGCA'G
Cuts at: 830 2993 3257 830
Size: 2163 264 3478
Fragments arranged by size:
3478 2163 264
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5905
PvuII CAG'CTG
Cuts at: 3002 3002
Size: 5905
SacI G_AGCT'C
Cuts at: 2817 2817
Size: 5905
SalI G'TCGA_C
Cuts at: 1936 3247 1936
Size: 1311 4594
SbfI CC_TGCA'GG
Cuts at: 3257 3257
Size: 5905
ScaI AGT'ACT
Cuts at: 4153 5865 4153
Size: 1712 4193
SfcI C'TryA_G
Cuts at: 826 1045 2426 2989 3253 4388 5069 5260 826
Size: 219 1381 563 264 1135 681 191 1471
Fragments arranged by size:
1471 1381 1135 681 563 264 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5905
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5905
SmaI CCC'GGG
Cuts at: 1872 3232 1872
Size: 1360 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2402 2698 4009 4910 5172 820
Size: 197 926 459 296 1311 901 262 1553
Fragments arranged by size:
1553 1311 926 901 459 296 262 197
SnaBI TAC'GTA
Cuts at: 493 3696 493
Size: 3203 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5905
SphI G_CATG'C
Cuts at: 1132 3263 1132
Size: 2131 3774
SspI AAT'ATT
Cuts at: 5 52 2247 3121 3829 5
Size: 47 2195 874 708 2081
Fragments arranged by size:
2195 2081 874 708 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5905
StyI C'CwwG_G
Cuts at: 513 1065 2153 2332 2584 3344 513
Size: 552 1088 179 252 760 3074
Fragments arranged by size:
3074 1088 760 552 252 179
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3932 3949 4102 4287 4732 325
Size: 1255 2352 17 153 185 445 1498
Fragments arranged by size:
2352 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2461 2863 4151
Size: 274 80 33 51 1443 484 402 1288
Cuts at: 4151 5863 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1288 484 402 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5865 5897 5865
Size: 32 5873
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5905
XbaI T'CTAG_A
Cuts at: 2126 3241 2126
Size: 1115 4790
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5905
XmaI C'CCGG_G
Cuts at: 1870 3230 1870
Size: 1360 4545
XmnI GAAnn'nnTTC
Cuts at: 2772 3021 4034 2772
Size: 249 1013 4643
Fragments arranged by size:
4643 1013 249
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3713 276
Size: 53 83 186 936 2179 2468
Fragments arranged by size:
2468 2179 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoNI EcoO109I FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI