Restriction map for
pFN21AB8114
(Circular) (Six-base) MAPSORT of: pFN21AB8114.seq Check: 4074 from: 1 to: 5677
pFN21AB8114 5677 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2816 2816
Size: 5677
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3487 278
Size: 53 83 186 936 1951 2468
Fragments arranged by size:
2468 1951 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3020 1937
Size: 1083 4594
Acc65I G'GTAC_C
Cuts at: 2998 2998
Size: 5677
AclI AA'CG_TT
Cuts at: 1563 3804 4177 1563
Size: 2241 373 3063
Fragments arranged by size:
3063 2241 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2366 2458 2624 3740 5124 5392 1632
Size: 734 92 166 1116 1384 268 1917
Fragments arranged by size:
1917 1384 1116 734 268 166 92
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5480
AflIII A'CryG_T
Cuts at: 1203 4576 5553 1203
Size: 3373 977 1327
Fragments arranged by size:
3373 1327 977
AgeI A'CCGG_T
Cuts at: 4508 4508
Size: 5677
AhdI GACnn_n'nnGTC
Cuts at: 4406 4406
Size: 5677
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5677
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2739 1184
Size: 1555 4122
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2378 2410 2378
Size: 32 5645
AlwNI CAG_nnn'CTG
Cuts at: 2237 2258 3420 4992 2237
Size: 21 1162 1572 2922
Fragments arranged by size:
2922 1572 1162 21
ApaI G_GGCC'C
Cuts at: 2369 2369
Size: 5677
ApaLI G'TGCA_C
Cuts at: 3734 4890 3734
Size: 1156 4521
ApoI r'AATT_y
Cuts at: 1493 2138 2318 2986 3212 3364 5473 1493
Size: 645 180 668 226 152 2109 1697
Fragments arranged by size:
2109 1697 668 645 226 180 152
AseI AT'TA_AT
Cuts at: 160 1032 4231 160
Size: 872 3199 1606
Fragments arranged by size:
3199 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5677
AvaI C'yCGr_G
Cuts at: 1870 1943 2247 2300 3002 1870
Size: 73 304 53 702 4545
Fragments arranged by size:
4545 702 304 73 53
BamHI G'GATC_C
Cuts at: 3007 3374 3007
Size: 367 5310
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2094 2998 4453 618
Size: 325 217 627 307 904 1455 1842
Fragments arranged by size:
1842 1455 904 627 325 307 217
BanII G_rGCy'C
Cuts at: 1391 2352 2369 2996 1391
Size: 961 17 627 4072
Fragments arranged by size:
4072 961 627 17
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2302 928
Size: 971 403 4303
Fragments arranged by size:
4303 971 403
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3868 3902 1903
Size: 34 1931 34 3678
Fragments arranged by size:
3678 1931 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3569 4779 3569
Size: 1210 4467
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5677
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4288 136
Size: 107 122 71 631 605 2616 1525
Fragments arranged by size:
2616 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5672 5672
Size: 5677
BlpI GC'TnA_GC
Cuts at: 2657 3094 2657
Size: 437 5240
Bme1580I G_kGCm'C
Cuts at: 1790 2369 2445 2705 3738 4894 1790
Size: 579 76 260 1033 1156 2573
Fragments arranged by size:
2573 1156 1033 579 260 76
BmgBI CAC'GTC
Cuts at: 1318 2032 1318
Size: 714 4963
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2654 3408 4366
Size: 416 53 83 399 24 1237 754 958
Cuts at: 4366 442
Size: 1753
Fragments arranged by size:
1753 1237 958 754 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5677
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2974 2974
Size: 5677
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2072 4337 1239
Size: 833 2265 2579
Fragments arranged by size:
2579 2265 833
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3802 4667 4965 3802
Size: 865 298 4514
Fragments arranged by size:
4514 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2328 4340 882
Size: 617 829 2012 2219
Fragments arranged by size:
2219 2012 829 617
BsaAI yAC'GTr
Cuts at: 493 1204 3468 493
Size: 711 2264 2702
Fragments arranged by size:
2702 2264 711
BsaBI GATnn'nnATC
Cuts at: 5671 5671
Size: 5677
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2533 3484 3866 4501
Size: 53 83 186 936 1000 951 382 635
Cuts at: 4501 275
Size: 1451
Fragments arranged by size:
1451 1000 951 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4110 4508 4782 4929 5340 4110
Size: 398 274 147 411 4447
Fragments arranged by size:
4447 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5452
Fragments arranged by size:
5452 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2318 2446 2449 2603 2963 1186
Size: 1132 128 3 154 360 3900
Fragments arranged by size:
3900 1132 360 154 128 3
BseYI C'CCAG_C
Cuts at: 2810 2871 4880 5376 2810
Size: 61 2009 496 3111
Fragments arranged by size:
3111 2009 496 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5104
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2203 3127 3888 4916 1540
Size: 275 186 202 924 761 1028 2301
Fragments arranged by size:
2301 1028 924 761 275 202 186
BsiHKAI G_wGCw'C
Cuts at: 2352 3738 3823 4894 2352
Size: 1386 85 1071 3135
Fragments arranged by size:
3135 1386 1071 85
BsmI GAATG_Cn'
Cuts at: 3188 3281 3188
Size: 93 5584
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2352 2369 2445 2705 2996 3738 3823
Size: 399 562 17 76 260 291 742 85
Cuts at: 3823 4894 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 562 399 291 260 85
76 17
BspEI T'CCGG_A
Cuts at: 5340 5340
Size: 5677
BspHI T'CATG_A
Cuts at: 3566 5296 3566
Size: 1730 3947
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2775 2816 3032 844
Size: 1931 41 216 3489
Fragments arranged by size:
3489 1931 216 41
BsrBI CCG'CTC
Cuts at: 1394 1823 2575 3564 1394
Size: 429 752 989 3507
Fragments arranged by size:
3507 989 752 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4172 4346 65
Size: 1183 2924 174 1396
Fragments arranged by size:
2924 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4321 4508 1700
Size: 110 2511 187 2869
Fragments arranged by size:
2869 2511 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5677
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5677
BssSI C'ACGA_G
Cuts at: 3737 4749 3737
Size: 1012 4665
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5677
BstBI TT'CG_AA
Cuts at: 4481 4481
Size: 5677
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5677
BstYI r'GATC_y
Cuts at: 1972 2022 2881 3007 3374 3759 3776 5228 5314
Size: 50 859 126 367 385 17 1452 86
Cuts at: 5314 5326 5672 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 859 385 367 346 126 86
50 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2778 513
Size: 552 939 774 3412
Fragments arranged by size:
3412 939 774 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4938
Fragments arranged by size:
4938 683 56
BtsI GCAGTG_nn'
Cuts at: 2269 3193 3987 4007 2269
Size: 924 794 20 3939
Fragments arranged by size:
3939 924 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5677
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5642
DraI TTT'AAA
Cuts at: 2981 2981
Size: 5677
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4684 809
Size: 507 3368 1802
Fragments arranged by size:
3368 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2151 2209 2775
Size: 54 1425 50 275 18 321 58 566
Cuts at: 2775 3124 4013 5642 8
Size: 349 889 1629 43
Fragments arranged by size:
1629 1425 889 566 349 321 275 58
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3124 1537
Size: 275 1312 4090
Fragments arranged by size:
4090 1312 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2431 3607 1353
Size: 1078 1176 3423
Fragments arranged by size:
3423 1176 1078
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4264 4638 4784 387
Size: 1064 138 2675 374 146 1280
Fragments arranged by size:
2675 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2350 2350
Size: 5677
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2072 2366 2458 2624 3740 4337 5124
Size: 393 440 294 92 166 1116 597 787
Cuts at: 5124 5392 1239
Size: 268 1524
Fragments arranged by size:
1524 1116 787 597 440 393 294 268
166 92
EcoNI CCTnn'n_nnAGG
Cuts at: 2753 2753
Size: 5677
EcoO109I rG'GnC_Cy
Cuts at: 2366 2408 2764 2898 2366
Size: 42 356 134 5145
Fragments arranged by size:
5145 356 134 42
EcoRI G'AATT_C
Cuts at: 2318 2986 3364 2318
Size: 668 378 4631
Fragments arranged by size:
4631 668 378
FspI TGC'GCA
Cuts at: 4183 4183
Size: 5677
HaeII r_GCGC'y
Cuts at: 1678 4824 1678
Size: 3146 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2441 2473 2523 2555 4318
Size: 32 135 32 869 32 50 32 1763
Cuts at: 4318 4350 4392 4424 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1763 869 135 50 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3021 3267 669
Size: 988 281 1083 246 3079
Fragments arranged by size:
3079 1083 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5677
HpaI GTT'AAC
Cuts at: 3267 3267
Size: 5677
KpnI G_GTAC'C
Cuts at: 3002 3002
Size: 5677
MfeI C'AATT_G
Cuts at: 3276 3276
Size: 5677
MluI A'CGCG_T
Cuts at: 5553 5553
Size: 5677
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4791 4975 1305
Size: 3486 184 2007
Fragments arranged by size:
3486 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2777 10
Size: 54 1425 343 945 2910
Fragments arranged by size:
2910 1425 945 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3635 3994 4153 518
Size: 648 2469 359 159 2042
Fragments arranged by size:
2469 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2051 2602 2773 2810 3093 3770 4918 5163
Size: 445 551 171 37 283 677 1148 245
Cuts at: 5163 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 551 445 283 245 171
37
NcoI C'CATG_G
Cuts at: 513 1065 2004 2778 513
Size: 552 939 774 3412
Fragments arranged by size:
3412 939 774 552
NdeI CA'TA_TG
Cuts at: 387 2161 387
Size: 1774 3903
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5677
NotI GC'GGCC_GC
Cuts at: 3124 3124
Size: 5677
NruI TCG'CGA
Cuts at: 2667 2667
Size: 5677
NspI r_CATG'y
Cuts at: 1132 1229 3035 4580 1132
Size: 97 1806 1545 2229
Fragments arranged by size:
2229 1806 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2438 1348
Size: 1090 4587
PciI A'CATG_T
Cuts at: 4576 4576
Size: 5677
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5677
PfoI T'CCnGG_A
Cuts at: 2313 2313
Size: 5677
PmeI GTTT'AAAC
Cuts at: 2981 2981
Size: 5677
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3746 3778 5259 5291 3746
Size: 32 1481 32 4132
Fragments arranged by size:
4132 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2764 2898 2764
Size: 134 5543
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5677
PsiI TTA'TAA
Cuts at: 3247 3247
Size: 5677
PspOMI G'GGCC_C
Cuts at: 2365 2365
Size: 5677
PstI C_TGCA'G
Cuts at: 830 2772 3029 830
Size: 1942 257 3478
Fragments arranged by size:
3478 1942 257
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5677
PvuII CAG'CTG
Cuts at: 2602 2773 2810 2602
Size: 171 37 5469
Fragments arranged by size:
5469 171 37
SacI G_AGCT'C
Cuts at: 2352 2352
Size: 5677
SalI G'TCGA_C
Cuts at: 1936 3019 1936
Size: 1083 4594
SanDI GG'GwC_CC
Cuts at: 2898 2898
Size: 5677
SbfI CC_TGCA'GG
Cuts at: 3029 3029
Size: 5677
ScaI AGT'ACT
Cuts at: 3925 5637 3925
Size: 1712 3965
SfcI C'TryA_G
Cuts at: 826 1045 2768 2785 3025 4160 4841 5032 826
Size: 219 1723 17 240 1135 681 191 1471
Fragments arranged by size:
1723 1471 1135 681 240 219 191 17
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5677
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5677
SmaI CCC'GGG
Cuts at: 1872 3004 1872
Size: 1132 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3781 4682 4944 820
Size: 197 926 1838 901 262 1553
Fragments arranged by size:
1838 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3468 493
Size: 2975 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5677
SphI G_CATG'C
Cuts at: 1132 3035 1132
Size: 1903 3774
SspI AAT'ATT
Cuts at: 5 52 3601 5
Size: 47 3549 2081
Fragments arranged by size:
3549 2081 47
StuI AGG'CCT
Cuts at: 1517 2957 1517
Size: 1440 4237
StyI C'CwwG_G
Cuts at: 513 1065 2004 2527 2736 2778 2865 3116 513
Size: 552 939 523 209 42 87 251 3074
Fragments arranged by size:
3074 939 552 523 251 209 87 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2112 3704 3721 3874 4059 4504 325
Size: 1255 532 1592 17 153 185 445 1498
Fragments arranged by size:
1592 1498 1255 532 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3923 5635 96
Size: 274 80 33 51 1443 1946 1712 138
Fragments arranged by size:
1946 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5637 5669 5637
Size: 32 5645
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5677
XbaI T'CTAG_A
Cuts at: 3013 3013
Size: 5677
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5677
XmaI C'CCGG_G
Cuts at: 1870 3002 1870
Size: 1132 4545
XmnI GAAnn'nnTTC
Cuts at: 3806 3806
Size: 5677
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3485 276
Size: 53 83 186 936 1951 2468
Fragments arranged by size:
2468 1951 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NruI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI
PmlI PspXI PsrI RsrII SacII SapI SexAI SfiI
SfoI SrfI SwaI XcmI