Restriction map for
pFN21AB8113
(Circular) (Six-base) MAPSORT of: pFN21AB8113.seq Check: 4526 from: 1 to: 5845
pFN21AB8113 5845 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2284 2792 2284
Size: 508 5337
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3655 278
Size: 53 83 186 936 2119 2468
Fragments arranged by size:
2468 2119 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2100 3188 1937
Size: 163 1088 4594
Fragments arranged by size:
4594 1088 163
Acc65I G'GTAC_C
Cuts at: 3166 3166
Size: 5845
AclI AA'CG_TT
Cuts at: 1563 3972 4345 1563
Size: 2409 373 3063
Fragments arranged by size:
3063 2409 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2572 3908 5292 5560 1632
Size: 940 1336 1384 268 1917
Fragments arranged by size:
1917 1384 1336 940 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5648
AflIII A'CryG_T
Cuts at: 1203 2095 4744 5721 1203
Size: 892 2649 977 1327
Fragments arranged by size:
2649 1327 977 892
AgeI A'CCGG_T
Cuts at: 4676 4676
Size: 5845
AhdI GACnn_n'nnGTC
Cuts at: 4574 4574
Size: 5845
AleI CACnn'nnGTG
Cuts at: 1166 2870 1166
Size: 1704 4141
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5845
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2619 2651 2619
Size: 32 5813
AlwNI CAG_nnn'CTG
Cuts at: 2947 3588 5160 2947
Size: 641 1572 3632
Fragments arranged by size:
3632 1572 641
ApaLI G'TGCA_C
Cuts at: 3902 5058 3902
Size: 1156 4689
ApoI r'AATT_y
Cuts at: 1493 3154 3380 3532 5641 1493
Size: 1661 226 152 2109 1697
Fragments arranged by size:
2109 1697 1661 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4399 160
Size: 872 3367 1606
Fragments arranged by size:
3367 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5845
AvaI C'yCGr_G
Cuts at: 1870 1943 3170 1870
Size: 73 1227 4545
Fragments arranged by size:
4545 1227 73
BamHI G'GATC_C
Cuts at: 2853 3175 3542 2853
Size: 322 367 5156
Fragments arranged by size:
5156 367 322
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2236 3166 4621 618
Size: 325 217 627 449 930 1455 1842
Fragments arranged by size:
1842 1455 930 627 449 325 217
BanII G_rGCy'C
Cuts at: 1391 2331 2889 3050 3164 1391
Size: 940 558 161 114 4072
Fragments arranged by size:
4072 940 558 161 114
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2088 2797 928
Size: 971 189 709 3976
Fragments arranged by size:
3976 971 709 189
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4036 4070 1903
Size: 34 50 34 2015 34 3678
Fragments arranged by size:
3678 2015 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3737 4947 3737
Size: 1210 4635
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5845
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2456 4456 136
Size: 107 122 71 631 605 784 2000 1525
Fragments arranged by size:
2000 1525 784 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2116 5840 2116
Size: 3724 2121
BlpI GC'TnA_GC
Cuts at: 3262 3262
Size: 5845
Bme1580I G_kGCm'C
Cuts at: 1790 2239 3906 5062 1790
Size: 449 1667 1156 2573
Fragments arranged by size:
2573 1667 1156 449
BmgBI CAC'GTC
Cuts at: 1318 2096 1318
Size: 778 5067
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2379 2533 3576
Size: 416 53 83 399 24 962 154 1043
Cuts at: 3576 4534 442
Size: 958 1753
Fragments arranged by size:
1753 1043 962 958 416 399 154 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5845
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1994 2437 2762 2903 4505 1239
Size: 755 443 325 141 1602 2579
Fragments arranged by size:
2579 1602 755 443 325 141
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2378 3970 4835 5133 2378
Size: 1592 865 298 3090
Fragments arranged by size:
3090 1592 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4508 882
Size: 617 3009 2219
Fragments arranged by size:
3009 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2182 3636 493
Size: 711 978 1454 2702
Fragments arranged by size:
2702 1454 978 711
BsaBI GATnn'nnATC
Cuts at: 5839 5839
Size: 5845
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3652 4034 4669 275
Size: 53 83 186 936 2119 382 635 1451
Fragments arranged by size:
2119 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4278 4676 4950 5097 5508 4278
Size: 398 274 147 411 4615
Fragments arranged by size:
4615 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5620
Fragments arranged by size:
5620 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2381 3074 3093 1186
Size: 1195 693 19 3938
Fragments arranged by size:
3938 1195 693 19
BseYI C'CCAG_C
Cuts at: 2696 3071 5048 5544 2696
Size: 375 1977 496 2997
Fragments arranged by size:
2997 1977 496 375
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5272
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3295 4056 5084 1540
Size: 275 186 1294 761 1028 2301
Fragments arranged by size:
2301 1294 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2528 2889 3050 3906 3991 5062 2528
Size: 361 161 856 85 1071 3311
Fragments arranged by size:
3311 1071 856 361 161 85
BsmI GAATG_Cn'
Cuts at: 3356 3449 3356
Size: 93 5752
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2239 2331 2528 2889 3050 3164 3906
Size: 399 449 92 197 361 161 114 742
Cuts at: 3906 3991 5062 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 449 399 361 197 161
114 92 85
BspEI T'CCGG_A
Cuts at: 5508 5508
Size: 5845
BspHI T'CATG_A
Cuts at: 3734 5464 3734
Size: 1730 4115
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2284 2745 2792 3200 844
Size: 1440 461 47 408 3489
Fragments arranged by size:
3489 1440 461 408 47
BsrBI CCG'CTC
Cuts at: 1394 1823 2325 3732 1394
Size: 429 502 1407 3507
Fragments arranged by size:
3507 1407 502 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2445 2592 2860 4340 4514 65
Size: 1183 1197 147 268 1480 174 1396
Fragments arranged by size:
1480 1396 1197 1183 268 174 147
BsrFI r'CCGG_y
Cuts at: 1700 1810 2379 2450 3090 4489 4676 1700
Size: 110 569 71 640 1399 187 2869
Fragments arranged by size:
2869 1399 640 569 187 110 71
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5845
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5845
BssSI C'ACGA_G
Cuts at: 3905 4917 3905
Size: 1012 4833
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5845
BstBI TT'CG_AA
Cuts at: 4649 4649
Size: 5845
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5845
BstXI CCAn_nnnn'nTGG
Cuts at: 2296 2296
Size: 5845
BstYI r'GATC_y
Cuts at: 1972 2116 2853 3175 3542 3927 3944 5396 5482
Size: 144 737 322 367 385 17 1452 86
Cuts at: 5482 5494 5840 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 737 385 367 346 322 144
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5293
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5106
Fragments arranged by size:
5106 683 56
BtsI GCAGTG_nn'
Cuts at: 3031 3361 4155 4175 3031
Size: 330 794 20 4701
Fragments arranged by size:
4701 794 330 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5845
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5810
DraI TTT'AAA
Cuts at: 3149 3149
Size: 5845
DraIII CAC_nnn'GTG
Cuts at: 2644 2644
Size: 5845
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4852 809
Size: 507 3536 1802
Fragments arranged by size:
3536 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2335 2457 2646
Size: 54 1425 50 275 18 505 122 189
Cuts at: 2646 3106 3292 4181 5810 8
Size: 460 186 889 1629 43
Fragments arranged by size:
1629 1425 889 505 460 275 189 186
122 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3292 1537
Size: 275 1480 4090
Fragments arranged by size:
4090 1480 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2153 2591 2731 3775 1353
Size: 800 438 140 1044 3423
Fragments arranged by size:
3423 1044 800 438 140
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4432 4806 4952 387
Size: 1064 138 2843 374 146 1280
Fragments arranged by size:
2843 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2887 3048 2887
Size: 161 5684
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1994 2437 2572 2762 2903 3908 4505
Size: 393 362 443 135 190 141 1005 597
Cuts at: 4505 5292 5560 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1005 787 597 443 393 362 268
190 141 135
EcoRI G'AATT_C
Cuts at: 3154 3532 3154
Size: 378 5467
EcoRV GAT'ATC
Cuts at: 2513 2513
Size: 5845
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2324 2324
Size: 5845
FspI TGC'GCA
Cuts at: 2978 4351 2978
Size: 1373 4472
HaeII r_GCGC'y
Cuts at: 1678 4992 1678
Size: 3314 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2297 2329 2823 2855 4486
Size: 32 135 32 725 32 494 32 1631
Cuts at: 4486 4518 4560 4592 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1631 725 494 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3189 3435 669
Size: 988 281 1251 246 3079
Fragments arranged by size:
3079 1251 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5845
HpaI GTT'AAC
Cuts at: 3435 3435
Size: 5845
KpnI G_GTAC'C
Cuts at: 3170 3170
Size: 5845
MfeI C'AATT_G
Cuts at: 3444 3444
Size: 5845
MluI A'CGCG_T
Cuts at: 5721 5721
Size: 5845
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4959 5143 1305
Size: 3654 184 2007
Fragments arranged by size:
3654 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2648 3108 10
Size: 54 1425 343 816 460 2747
Fragments arranged by size:
2747 1425 816 460 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2870 3803 4162 4321 518
Size: 648 1704 933 359 159 2042
Fragments arranged by size:
2042 1704 933 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2415 2944 3071 3261 3938 5086 5331 1606
Size: 809 529 127 190 677 1148 245 2120
Fragments arranged by size:
2120 1148 809 677 529 245 190 127
NaeI GCC'GGC
Cuts at: 2452 3092 2452
Size: 640 5205
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5293
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5845
NgoMIV G'CCGG_C
Cuts at: 2450 3090 2450
Size: 640 5205
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5845
NotI GC'GGCC_GC
Cuts at: 3292 3292
Size: 5845
NspI r_CATG'y
Cuts at: 1132 1229 3203 4748 1132
Size: 97 1974 1545 2229
Fragments arranged by size:
2229 1974 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5845
PciI A'CATG_T
Cuts at: 4744 4744
Size: 5845
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2644 1108
Size: 1536 4309
PmeI GTTT'AAAC
Cuts at: 3149 3149
Size: 5845
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2339 2371 3914 3946 5427 5459 2339
Size: 32 1543 32 1481 32 2725
Fragments arranged by size:
2725 1543 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5845
PsiI TTA'TAA
Cuts at: 3415 3415
Size: 5845
PstI C_TGCA'G
Cuts at: 830 3070 3197 830
Size: 2240 127 3478
Fragments arranged by size:
3478 2240 127
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5845
PvuII CAG'CTG
Cuts at: 2415 2944 3071 2415
Size: 529 127 5189
Fragments arranged by size:
5189 529 127
SacI G_AGCT'C
Cuts at: 2889 3050 2889
Size: 161 5684
SalI G'TCGA_C
Cuts at: 1936 3187 1936
Size: 1251 4594
SapI GCTCTTCn'nnn_
Cuts at: 2153 2591 2153
Size: 438 5407
SbfI CC_TGCA'GG
Cuts at: 3197 3197
Size: 5845
ScaI AGT'ACT
Cuts at: 4093 5805 4093
Size: 1712 4133
SfcI C'TryA_G
Cuts at: 826 1045 2939 3066 3193 4328 5009 5200 826
Size: 219 1894 127 127 1135 681 191 1471
Fragments arranged by size:
1894 1471 1135 681 219 191 127 127
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5845
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5845
SmaI CCC'GGG
Cuts at: 1872 3172 1872
Size: 1300 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2393 3949 4850 5112 820
Size: 197 926 450 1556 901 262 1553
Fragments arranged by size:
1556 1553 926 901 450 262 197
SnaBI TAC'GTA
Cuts at: 493 3636 493
Size: 3143 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5845
SphI G_CATG'C
Cuts at: 1132 3203 1132
Size: 2071 3774
SspI AAT'ATT
Cuts at: 5 52 3769 5
Size: 47 3717 2081
Fragments arranged by size:
3717 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5845
StyI C'CwwG_G
Cuts at: 513 1065 2253 3284 513
Size: 552 1188 1031 3074
Fragments arranged by size:
3074 1188 1031 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2100 3872 3889 4042 4227 4672 325
Size: 1255 520 1772 17 153 185 445 1498
Fragments arranged by size:
1772 1498 1255 520 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4091 5803 96
Size: 274 80 33 51 1443 2114 1712 138
Fragments arranged by size:
2114 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2030 2062 5805 5837 2030
Size: 32 3743 32 2038
Fragments arranged by size:
3743 2038 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5845
XbaI T'CTAG_A
Cuts at: 2077 3181 2077
Size: 1104 4741
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2251 2251
Size: 5845
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5845
XmaI C'CCGG_G
Cuts at: 1870 3170 1870
Size: 1300 4545
XmnI GAAnn'nnTTC
Cuts at: 3974 3974
Size: 5845
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3653 276
Size: 53 83 186 936 2119 2468
Fragments arranged by size:
2468 2119 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoO109I
FseI FspAI KasI NarI NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI
SexAI SfiI SfoI SrfI SwaI