Restriction map for
pFN21AB8104
(Circular) (Six-base) MAPSORT of: pFN21AB8104.seq Check: 8044 from: 1 to: 6088
pFN21AB8104 6088 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2352 3055 2352
Size: 703 5385
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3898 278
Size: 53 83 186 936 2362 2468
Fragments arranged by size:
2468 2362 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3431 1937
Size: 1494 4594
Acc65I G'GTAC_C
Cuts at: 3409 3409
Size: 6088
AclI AA'CG_TT
Cuts at: 1563 4215 4588 1563
Size: 2652 373 3063
Fragments arranged by size:
3063 2652 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2648 2916 3157 4151 5535 5803 1632
Size: 1016 268 241 994 1384 268 1917
Fragments arranged by size:
1917 1384 1016 994 268 268 241
AfeI AGC'GCT
Cuts at: 2558 2633 2651 2558
Size: 75 18 5995
Fragments arranged by size:
5995 75 18
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5891
AflIII A'CryG_T
Cuts at: 1203 2865 4987 5964 1203
Size: 1662 2122 977 1327
Fragments arranged by size:
2122 1662 1327 977
AgeI A'CCGG_T
Cuts at: 4919 4919
Size: 6088
AhdI GACnn_n'nnGTC
Cuts at: 4817 4817
Size: 6088
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6088
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6088
AlwNI CAG_nnn'CTG
Cuts at: 2755 3289 3831 5403 2755
Size: 534 542 1572 3440
Fragments arranged by size:
3440 1572 542 534
ApaI G_GGCC'C
Cuts at: 2024 2083 2173 2024
Size: 59 90 5939
Fragments arranged by size:
5939 90 59
ApaLI G'TGCA_C
Cuts at: 3212 4145 5301 3212
Size: 933 1156 3999
Fragments arranged by size:
3999 1156 933
ApoI r'AATT_y
Cuts at: 1493 3397 3623 3775 5884 1493
Size: 1904 226 152 2109 1697
Fragments arranged by size:
2109 1904 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4642 160
Size: 872 3610 1606
Fragments arranged by size:
3610 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6088
AvaI C'yCGr_G
Cuts at: 1870 1943 2015 2065 2166 2405 3413 1870
Size: 73 72 50 101 239 1008 4545
Fragments arranged by size:
4545 1008 239 101 73 72 50
BamHI G'GATC_C
Cuts at: 2890 3418 3785 2890
Size: 528 367 5193
Fragments arranged by size:
5193 528 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2609 3409 4864 618
Size: 325 217 627 822 800 1455 1842
Fragments arranged by size:
1842 1455 822 800 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2024 2083 2166 2173 2224 2271 2336 3407
Size: 633 59 83 7 51 47 65 1071
Cuts at: 3407 1391
Size: 4072
Fragments arranged by size:
4072 1071 633 83 65 59 51 47
7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5117
BbvCI CC'TCA_GC
Cuts at: 2244 3113 2244
Size: 869 5219
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2968 3002 3053 3087 4279 4313 1903
Size: 34 1031 34 51 34 1192 34 3678
Fragments arranged by size:
3678 1192 1031 51 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3172 3980 5190 3172
Size: 808 1210 4070
Fragments arranged by size:
4070 1210 808
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6088
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2028 2177 3377
Size: 107 122 71 631 605 356 149 1200
Cuts at: 3377 4699 136
Size: 1322 1525
Fragments arranged by size:
1525 1322 1200 631 605 356 149 122
107 71
BglII A'GATC_T
Cuts at: 6083 6083
Size: 6088
BlpI GC'TnA_GC
Cuts at: 2429 3505 2429
Size: 1076 5012
Bme1580I G_kGCm'C
Cuts at: 1790 2024 2083 2173 2280 2586 2612 3216 4149
Size: 234 59 90 107 306 26 604 933
Cuts at: 4149 5305 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 933 604 306 234 107 90
59 26
BmgBI CAC'GTC
Cuts at: 1318 2275 2485 1318
Size: 957 210 4921
Fragments arranged by size:
4921 957 210
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2598 3819 4777
Size: 416 53 83 399 24 1181 1221 958
Cuts at: 4777 442
Size: 1753
Fragments arranged by size:
1753 1221 1181 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6088
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2093 2959 3176 4748 1239
Size: 854 866 217 1572 2579
Fragments arranged by size:
2579 1572 866 854 217
Bpu10I CC'TnA_GC
Cuts at: 2244 3113 2244
Size: 869 5219
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4213 5078 5376 4213
Size: 865 298 4925
Fragments arranged by size:
4925 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2383 4751 882
Size: 617 884 2368 2219
Fragments arranged by size:
2368 2219 884 617
BsaAI yAC'GTr
Cuts at: 493 1204 3879 493
Size: 711 2675 2702
Fragments arranged by size:
2702 2675 711
BsaBI GATnn'nnATC
Cuts at: 6082 6082
Size: 6088
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2802 3895 4277 4912
Size: 53 83 186 936 1269 1093 382 635
Cuts at: 4912 275
Size: 1451
Fragments arranged by size:
1451 1269 1093 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2514 4521 4919 5193 5340 5751 2514
Size: 2007 398 274 147 411 2851
Fragments arranged by size:
2851 2007 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5863
Fragments arranged by size:
5863 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2279 2371 2383 2834 2837 2924 2927 3194
Size: 1093 92 12 451 3 87 3 267
Cuts at: 3194 3326 1186
Size: 132 3948
Fragments arranged by size:
3948 1093 451 267 132 92 87 12
3 3
BseYI C'CCAG_C
Cuts at: 3340 5291 5787 3340
Size: 1951 496 3641
Fragments arranged by size:
3641 1951 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2483 2591 1163
Size: 573 747 108 4660
Fragments arranged by size:
4660 747 573 108
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2042 2139 2148 3538 4299 5327
Size: 275 186 41 97 9 1390 761 1028
Cuts at: 5327 1540
Size: 2301
Fragments arranged by size:
2301 1390 1028 761 275 186 97 41
9
BsiHKAI G_wGCw'C
Cuts at: 2787 2919 3216 4149 4234 5305 2787
Size: 132 297 933 85 1071 3570
Fragments arranged by size:
3570 1071 933 297 132 85
BsmI GAATG_Cn'
Cuts at: 3599 3692 3599
Size: 93 5995
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2024 2083 2166 2173 2224 2271 2280
Size: 399 234 59 83 7 51 47 9
Cuts at: 2280 2336 2586 2612 2787 2919 3216 3407 4149
Size: 56 250 26 175 132 297 191 742
Cuts at: 4149 4234 5305 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 297 250 234 191
175 132 85 83 59 56 51 47
26 9 7
BspEI T'CCGG_A
Cuts at: 2514 5751 2514
Size: 3237 2851
BspHI T'CATG_A
Cuts at: 3977 5707 3977
Size: 1730 4358
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2352 2433 2748 2763 3055 3443 844
Size: 1508 81 315 15 292 388 3489
Fragments arranged by size:
3489 1508 388 315 292 81 15
BsrBI CCG'CTC
Cuts at: 1394 1823 2077 2305 2845 3975 1394
Size: 429 254 228 540 1130 3507
Fragments arranged by size:
3507 1130 540 429 254 228
BsrDI GCAATG_nn'
Cuts at: 65 1248 4583 4757 65
Size: 1183 3335 174 1396
Fragments arranged by size:
3335 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2533 3233 3297 4732 4919 1700
Size: 110 723 700 64 1435 187 2869
Fragments arranged by size:
2869 1435 723 700 187 110 64
BsrGI T'GTAC_A
Cuts at: 96 2868 96
Size: 2772 3316
BssHII G'CGCG_C
Cuts at: 1925 2123 2926 1925
Size: 198 803 5087
Fragments arranged by size:
5087 803 198
BssSI C'ACGA_G
Cuts at: 3182 4148 5160 3182
Size: 966 1012 4110
Fragments arranged by size:
4110 1012 966
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2755 794
Size: 1961 4127
BstBI TT'CG_AA
Cuts at: 4892 4892
Size: 6088
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6088
BstYI r'GATC_y
Cuts at: 1972 2511 2890 3141 3285 3418 3785 4170 4187
Size: 539 379 251 144 133 367 385 17
Cuts at: 4187 5639 5725 5737 6083 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 539 385 379 367 346 251
144 133 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2154 2173 2216 2452 2520 2707 2904
Size: 552 1089 19 43 236 68 187 197
Cuts at: 2904 513
Size: 3697
Fragments arranged by size:
3697 1089 552 236 197 187 68 43
19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3212 487
Size: 683 56 1986 3363
Fragments arranged by size:
3363 1986 683 56
BtsI GCAGTG_nn'
Cuts at: 2960 3220 3604 4398 4418 2960
Size: 260 384 794 20 4630
Fragments arranged by size:
4630 794 384 260 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6088
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2170 2205 529
Size: 35 1606 35 4412
Fragments arranged by size:
4412 1606 35 35
DraI TTT'AAA
Cuts at: 3392 3392
Size: 6088
DraIII CAC_nnn'GTG
Cuts at: 2713 3187 2713
Size: 474 5614
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5095 809
Size: 507 3779 1802
Fragments arranged by size:
3779 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2039 2145 3280
Size: 54 1425 50 275 18 209 106 1135
Cuts at: 3280 3369 3535 4424 6053 8
Size: 89 166 889 1629 43
Fragments arranged by size:
1629 1425 1135 889 275 209 166 106
89 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2039 2145 3535 1537
Size: 275 227 106 1390 4090
Fragments arranged by size:
4090 1390 275 227 106
EarI CTCTTCn'nnn_
Cuts at: 1353 2120 2341 3078 4018 1353
Size: 767 221 737 940 3423
Fragments arranged by size:
3423 940 767 737 221
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4675 5049 5195 387
Size: 1064 138 3086 374 146 1280
Fragments arranged by size:
3086 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2093 2648 2916 2959 3157 3176 4151
Size: 393 461 555 268 43 198 19 975
Cuts at: 4151 4748 5535 5803 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 975 787 597 555 461 393 268
268 198 43 19
EcoNI CCTnn'n_nnAGG
Cuts at: 2819 2819
Size: 6088
EcoO109I rG'GnC_Cy
Cuts at: 2020 2055 2080 2200 2752 2020
Size: 35 25 120 552 5356
Fragments arranged by size:
5356 552 120 35 25
EcoRI G'AATT_C
Cuts at: 3397 3775 3397
Size: 378 5710
FspI TGC'GCA
Cuts at: 4594 4594
Size: 6088
HaeII r_GCGC'y
Cuts at: 1678 2560 2635 2653 5235 1678
Size: 882 75 18 2582 2531
Fragments arranged by size:
2582 2531 882 75 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3106 3138 4729 4761 4803
Size: 32 135 32 1534 32 1591 32 42
Cuts at: 4803 4835 1373
Size: 32 2626
Fragments arranged by size:
2626 1591 1534 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3432 3678 669
Size: 988 281 1494 246 3079
Fragments arranged by size:
3079 1494 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6088
HpaI GTT'AAC
Cuts at: 3678 3678
Size: 6088
KpnI G_GTAC'C
Cuts at: 3413 3413
Size: 6088
MfeI C'AATT_G
Cuts at: 3687 3687
Size: 6088
MluI A'CGCG_T
Cuts at: 5964 5964
Size: 6088
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5202 5386 1305
Size: 3897 184 2007
Fragments arranged by size:
3897 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3282 3371 10
Size: 54 1425 343 1450 89 2727
Fragments arranged by size:
2727 1450 1425 343 89 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4046 4405 4564 518
Size: 648 2880 359 159 2042
Fragments arranged by size:
2880 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2156 2175 2218 2248 2330 2454 2467 2575
Size: 550 19 43 30 82 124 13 108
Cuts at: 2575 2616 2746 3504 4181 5329 5574 1606
Size: 41 130 758 677 1148 245 2120
Fragments arranged by size:
2120 1148 758 677 550 245 130 124
108 82 43 41 30 19 13
NaeI GCC'GGC
Cuts at: 3235 3235
Size: 6088
NcoI C'CATG_G
Cuts at: 513 1065 2520 513
Size: 552 1455 4081
Fragments arranged by size:
4081 1455 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6088
NgoMIV G'CCGG_C
Cuts at: 3233 3233
Size: 6088
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6088
NotI GC'GGCC_GC
Cuts at: 2145 3535 2145
Size: 1390 4698
NspI r_CATG'y
Cuts at: 1132 1229 2869 2953 3446 4991 1132
Size: 97 1640 84 493 1545 2229
Fragments arranged by size:
2229 1640 1545 493 97 84
PasI CC'CwG_GG
Cuts at: 1348 2291 3306 1348
Size: 943 1015 4130
Fragments arranged by size:
4130 1015 943
PciI A'CATG_T
Cuts at: 2865 4987 2865
Size: 2122 3966
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2713 3289 1108
Size: 1605 576 3907
Fragments arranged by size:
3907 1605 576
PfoI T'CCnGG_A
Cuts at: 3144 3288 3144
Size: 144 5944
PmeI GTTT'AAAC
Cuts at: 3392 3392
Size: 6088
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4157 4189 5670 5702 4157
Size: 32 1481 32 4543
Fragments arranged by size:
4543 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2200 2752 2200
Size: 552 5536
PshAI GACnn'nnGTC
Cuts at: 1613 2487 2522 1613
Size: 874 35 5179
Fragments arranged by size:
5179 874 35
PsiI TTA'TAA
Cuts at: 3658 3658
Size: 6088
PspOMI G'GGCC_C
Cuts at: 2020 2079 2169 2020
Size: 59 90 5939
Fragments arranged by size:
5939 90 59
PspXI vC'TCGA_Gb
Cuts at: 2405 2405
Size: 6088
PstI C_TGCA'G
Cuts at: 830 2063 2349 2745 2751 3032 3138 3440 830
Size: 1233 286 396 6 281 106 302 3478
Fragments arranged by size:
3478 1233 396 302 286 281 106 6
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6088
PvuII CAG'CTG
Cuts at: 2248 2467 2575 2746 2248
Size: 219 108 171 5590
Fragments arranged by size:
5590 219 171 108
SacII CC_GC'GG
Cuts at: 2157 2176 2219 2455 2157
Size: 19 43 236 5790
Fragments arranged by size:
5790 236 43 19
SalI G'TCGA_C
Cuts at: 1936 3430 1936
Size: 1494 4594
SapI GCTCTTCn'nnn_
Cuts at: 2120 2341 2120
Size: 221 5867
SbfI CC_TGCA'GG
Cuts at: 3440 3440
Size: 6088
ScaI AGT'ACT
Cuts at: 4336 6048 4336
Size: 1712 4376
SfcI C'TryA_G
Cuts at: 826 1045 2059 2345 2741 2747 3028 3134 3436
Size: 219 1014 286 396 6 281 106 302
Cuts at: 3436 4571 5252 5443 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1014 681 396 302 286 281
219 191 106 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 2177 2177
Size: 6088
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6088
SgrAI Cr'CCGG_yG
Cuts at: 1700 3233 3297 1700
Size: 1533 64 4491
Fragments arranged by size:
4491 1533 64
SmaI CCC'GGG
Cuts at: 1872 2017 2067 2168 3415 1872
Size: 145 50 101 1247 4545
Fragments arranged by size:
4545 1247 145 101 50
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2405 4192 5093 5355 820
Size: 197 926 462 1787 901 262 1553
Fragments arranged by size:
1787 1553 926 901 462 262 197
SnaBI TAC'GTA
Cuts at: 493 3879 493
Size: 3386 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6088
SphI G_CATG'C
Cuts at: 1132 3446 1132
Size: 2314 3774
SspI AAT'ATT
Cuts at: 5 52 4012 5
Size: 47 3960 2081
Fragments arranged by size:
3960 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6088
StyI C'CwwG_G
Cuts at: 513 1065 2520 2832 3527 513
Size: 552 1455 312 695 3074
Fragments arranged by size:
3074 1455 695 552 312
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2365 2557 4115 4132 4285 4470 4915
Size: 1255 785 192 1558 17 153 185 445
Cuts at: 4915 325
Size: 1498
Fragments arranged by size:
1558 1498 1255 785 445 192 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2868 4334 6046
Size: 274 80 33 51 1443 891 1466 1712
Cuts at: 6046 96
Size: 138
Fragments arranged by size:
1712 1466 1443 891 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2766 2798 6048 6080 2766
Size: 32 3250 32 2774
Fragments arranged by size:
3250 2774 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6088
XbaI T'CTAG_A
Cuts at: 3424 3424
Size: 6088
XhoI C'TCGA_G
Cuts at: 1943 2405 1943
Size: 462 5626
XmaI C'CCGG_G
Cuts at: 1870 2015 2065 2166 3413 1870
Size: 145 50 101 1247 4545
Fragments arranged by size:
4545 1247 145 101 50
XmnI GAAnn'nnTTC
Cuts at: 4217 4217
Size: 6088
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3896 276
Size: 53 83 186 936 2362 2468
Fragments arranged by size:
2468 2362 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SacII SalI SapI SbfI ScaI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI
FspAI KasI NarI NruI NsiI PacI PmlI PsrI
RsrII SacI SanDI SexAI SfoI SrfI SwaI XcmI