Restriction map for
pFN21AB8030
(Circular) (Six-base) MAPSORT of: pFN21AB8030.seq Check: 3688 from: 1 to: 6391
pFN21AB8030 6391 bp
With 182 enzymes: SgfI *
November 15, 2011 13:59 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4201 278
Size: 53 83 186 936 2665 2468
Fragments arranged by size:
2665 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3734 1937
Size: 1797 4594
Acc65I G'GTAC_C
Cuts at: 3712 3712
Size: 6391
AclI AA'CG_TT
Cuts at: 1563 4518 4891 1563
Size: 2955 373 3063
Fragments arranged by size:
3063 2955 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2333 2336 4454 5838 6106 1632
Size: 701 3 2118 1384 268 1917
Fragments arranged by size:
2118 1917 1384 701 268 3
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6194
AflIII A'CryG_T
Cuts at: 1203 3353 3664 5290 6267 1203
Size: 2150 311 1626 977 1327
Fragments arranged by size:
2150 1626 1327 977 311
AgeI A'CCGG_T
Cuts at: 5222 5222
Size: 6391
AhdI GACnn_n'nnGTC
Cuts at: 5120 5120
Size: 6391
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6391
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6391
AlwNI CAG_nnn'CTG
Cuts at: 3390 4134 5706 3390
Size: 744 1572 4075
Fragments arranged by size:
4075 1572 744
ApaLI G'TGCA_C
Cuts at: 4448 5604 4448
Size: 1156 5235
ApoI r'AATT_y
Cuts at: 1493 2032 2143 2816 3253 3375 3700 3926 4078
Size: 539 111 673 437 122 325 226 152
Cuts at: 4078 6187 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 673 539 437 325 226 152
122 111
AseI AT'TA_AT
Cuts at: 160 1032 4945 160
Size: 872 3913 1606
Fragments arranged by size:
3913 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6391
AvaI C'yCGr_G
Cuts at: 1870 1943 3716 1870
Size: 73 1773 4545
Fragments arranged by size:
4545 1773 73
BamHI G'GATC_C
Cuts at: 3478 3721 4088 3478
Size: 243 367 5781
Fragments arranged by size:
5781 367 243
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3712 5167 618
Size: 325 217 627 1925 1455 1842
Fragments arranged by size:
1925 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3710 1391
Size: 2319 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2066 2598 3498 928
Size: 971 167 532 900 3821
Fragments arranged by size:
3821 971 900 532 167
BbvCI CC'TCA_GC
Cuts at: 2152 2152
Size: 6391
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3605 3639 4582 4616 1903
Size: 34 1668 34 943 34 3678
Fragments arranged by size:
3678 1668 943 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3217 4283 5493 3217
Size: 1066 1210 4115
Fragments arranged by size:
4115 1210 1066
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6391
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2930 5002 136
Size: 107 122 71 631 605 1258 2072 1525
Fragments arranged by size:
2072 1525 1258 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6386 6386
Size: 6391
BlpI GC'TnA_GC
Cuts at: 3808 3808
Size: 6391
Bme1580I G_kGCm'C
Cuts at: 1790 2012 4452 5608 1790
Size: 222 2440 1156 2573
Fragments arranged by size:
2573 2440 1156 222
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6391
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3091 3179 3453
Size: 416 53 83 399 24 1674 88 274
Cuts at: 3453 4122 5080 442
Size: 669 958 1753
Fragments arranged by size:
1753 1674 958 669 416 399 274 88
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6391
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2421 3243 2421
Size: 822 5569
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3660 5051 1239
Size: 2421 1391 2579
Fragments arranged by size:
2579 2421 1391
Bpu10I CC'TnA_GC
Cuts at: 2152 2152
Size: 6391
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4516 5381 5679 4516
Size: 865 298 5228
Fragments arranged by size:
5228 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5054 882
Size: 617 3555 2219
Fragments arranged by size:
3555 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4182 493
Size: 711 2978 2702
Fragments arranged by size:
2978 2702 711
BsaBI GATnn'nnATC
Cuts at: 3366 6385 3366
Size: 3019 3372
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4198 4580 5215 275
Size: 53 83 186 936 2665 382 635 1451
Fragments arranged by size:
2665 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4824 5222 5496 5643 6054 4824
Size: 398 274 147 411 5161
Fragments arranged by size:
5161 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2859 2889 1178
Size: 30 165 30 1456 30 4680
Fragments arranged by size:
4680 1456 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3404 1186
Size: 2218 4173
BseYI C'CCAG_C
Cuts at: 2847 5594 6090 2847
Size: 2747 496 3148
Fragments arranged by size:
3148 2747 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2606 1163
Size: 573 870 4948
Fragments arranged by size:
4948 870 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3841 4602 5630 1540
Size: 275 186 1840 761 1028 2301
Fragments arranged by size:
2301 1840 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4452 4537 5608 4452
Size: 85 1071 5235
Fragments arranged by size:
5235 1071 85
BsmI GAATG_Cn'
Cuts at: 3565 3902 3995 3565
Size: 337 93 5961
Fragments arranged by size:
5961 337 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 3710 4452 4537 5608 1391
Size: 399 222 1698 742 85 1071 2174
Fragments arranged by size:
2174 1698 1071 742 399 222 85
BspEI T'CCGG_A
Cuts at: 6054 6054
Size: 6391
BspHI T'CATG_A
Cuts at: 4280 6010 4280
Size: 1730 4661
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3746 844
Size: 2902 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3412 4278 1394
Size: 429 1589 866 3507
Fragments arranged by size:
3507 1589 866 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3303 4886 5060 65
Size: 1183 2055 1583 174 1396
Fragments arranged by size:
2055 1583 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5035 5222 1700
Size: 110 3225 187 2869
Fragments arranged by size:
3225 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6391
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6391
BssSI C'ACGA_G
Cuts at: 4451 5463 4451
Size: 1012 5379
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3056 794
Size: 2262 4129
BstBI TT'CG_AA
Cuts at: 5195 5195
Size: 6391
BstEII G'GTnAC_C
Cuts at: 1184 3346 1184
Size: 2162 4229
BstYI r'GATC_y
Cuts at: 1972 3478 3721 4088 4473 4490 5942 6028 6040
Size: 1506 243 367 385 17 1452 86 12
Cuts at: 6040 6386 1972
Size: 346 1977
Fragments arranged by size:
1977 1506 1452 385 367 346 243 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5839
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2219 3463 487
Size: 683 56 993 1244 3415
Fragments arranged by size:
3415 1244 993 683 56
BtsI GCAGTG_nn'
Cuts at: 3207 3907 4701 4721 3207
Size: 700 794 20 4877
Fragments arranged by size:
4877 794 700 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6391
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3455 3490 529
Size: 35 2891 35 3430
Fragments arranged by size:
3430 2891 35 35
DraI TTT'AAA
Cuts at: 2611 2706 3695 2611
Size: 95 989 5307
Fragments arranged by size:
5307 989 95
DraIII CAC_nnn'GTG
Cuts at: 3187 3187
Size: 6391
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5398 809
Size: 507 4082 1802
Fragments arranged by size:
4082 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3838 4727 6356
Size: 54 1425 50 275 18 2008 889 1629
Cuts at: 6356 8
Size: 43
Fragments arranged by size:
2008 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3838 1537
Size: 275 2026 4090
Fragments arranged by size:
4090 2026 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2300 2355 2390 2994 4321 1353
Size: 947 55 35 604 1327 3423
Fragments arranged by size:
3423 1327 947 604 55 35
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3301 4978 5352 5498 387
Size: 1064 138 1712 1677 374 146 1280
Fragments arranged by size:
1712 1677 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2333 2336 3660 4454 5051 5838 6106
Size: 393 701 3 1324 794 597 787 268
Cuts at: 6106 1239
Size: 1524
Fragments arranged by size:
1524 1324 794 787 701 597 393 268
3
EcoNI CCTnn'n_nnAGG
Cuts at: 2299 2299
Size: 6391
EcoRI G'AATT_C
Cuts at: 2032 3253 3700 4078 2032
Size: 1221 447 378 4345
Fragments arranged by size:
4345 1221 447 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2849 3288 2849
Size: 439 5952
FspI TGC'GCA
Cuts at: 4897 4897
Size: 6391
HaeII r_GCGC'y
Cuts at: 1678 5538 1678
Size: 3860 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2080 2112 5032 5064 5106
Size: 32 135 32 508 32 2920 32 42
Cuts at: 5106 5138 1373
Size: 32 2626
Fragments arranged by size:
2920 2626 508 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3735 3981 669
Size: 988 281 1797 246 3079
Fragments arranged by size:
3079 1797 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6391
HpaI GTT'AAC
Cuts at: 3981 3981
Size: 6391
KpnI G_GTAC'C
Cuts at: 3716 3716
Size: 6391
MfeI C'AATT_G
Cuts at: 3990 3990
Size: 6391
MluI A'CGCG_T
Cuts at: 6267 6267
Size: 6391
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2537 2809 5505 5689 1305
Size: 1232 272 2696 184 2007
Fragments arranged by size:
2696 2007 1232 272 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4569
Fragments arranged by size:
4569 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3084 3234 4349 4708 4867 518
Size: 648 1918 150 1115 359 159 2042
Fragments arranged by size:
2042 1918 1115 648 359 159 150
MspA1I CmG'CkG
Cuts at: 1606 2156 3390 3654 3807 4484 5632 5877 1606
Size: 550 1234 264 153 677 1148 245 2120
Fragments arranged by size:
2120 1234 1148 677 550 264 245 153
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5839
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6391
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6391
NotI GC'GGCC_GC
Cuts at: 3838 3838
Size: 6391
NspI r_CATG'y
Cuts at: 1132 1229 3357 3668 3749 5294 1132
Size: 97 2128 311 81 1545 2229
Fragments arranged by size:
2229 2128 1545 311 97 81
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6391
PciI A'CATG_T
Cuts at: 3353 3664 5290 3353
Size: 311 1626 4454
Fragments arranged by size:
4454 1626 311
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6391
PfoI T'CCnGG_A
Cuts at: 3257 3257
Size: 6391
PmeI GTTT'AAAC
Cuts at: 3695 3695
Size: 6391
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4460 4492 5973 6005 4460
Size: 32 1481 32 4846
Fragments arranged by size:
4846 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6391
PsiI TTA'TAA
Cuts at: 3961 3961
Size: 6391
PstI C_TGCA'G
Cuts at: 830 3743 830
Size: 2913 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6391
PvuII CAG'CTG
Cuts at: 2156 3390 2156
Size: 1234 5157
SalI G'TCGA_C
Cuts at: 1936 3733 1936
Size: 1797 4594
SbfI CC_TGCA'GG
Cuts at: 3743 3743
Size: 6391
ScaI AGT'ACT
Cuts at: 4639 6351 4639
Size: 1712 4679
SexAI A'CCwGG_T
Cuts at: 3458 3458
Size: 6391
SfcI C'TryA_G
Cuts at: 826 1045 3739 4874 5555 5746 826
Size: 219 2694 1135 681 191 1471
Fragments arranged by size:
2694 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6391
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6391
SmaI CCC'GGG
Cuts at: 1872 3718 1872
Size: 1846 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4495 5396 5658 820
Size: 197 926 2552 901 262 1553
Fragments arranged by size:
2552 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4182 493
Size: 3689 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6391
SphI G_CATG'C
Cuts at: 1132 3749 1132
Size: 2617 3774
SspI AAT'ATT
Cuts at: 5 52 2760 4315 5
Size: 47 2708 1555 2081
Fragments arranged by size:
2708 2081 1555 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6391
StyI C'CwwG_G
Cuts at: 513 1065 2121 3830 513
Size: 552 1056 1709 3074
Fragments arranged by size:
3074 1709 1056 552
SwaI ATTT'AAAT
Cuts at: 2706 2706
Size: 6391
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3198 3633 4418 4435 4588 4773 5218
Size: 1255 1618 435 785 17 153 185 445
Cuts at: 5218 325
Size: 1498
Fragments arranged by size:
1618 1498 1255 785 445 435 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4637 6349 96
Size: 274 80 33 51 1443 2660 1712 138
Fragments arranged by size:
2660 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3546 3578 6351 6383 3546
Size: 32 2773 32 3554
Fragments arranged by size:
3554 2773 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3497 1316
Size: 2181 4210
XbaI T'CTAG_A
Cuts at: 3727 3727
Size: 6391
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2269 3151 2269
Size: 882 5509
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6391
XmaI C'CCGG_G
Cuts at: 1870 3716 1870
Size: 1846 4545
XmnI GAAnn'nnTTC
Cuts at: 4520 4520
Size: 6391
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4199 276
Size: 53 83 186 936 2665 2468
Fragments arranged by size:
2665 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SfiI SfoI SrfI