Restriction map for
pFN21AB8018
(Circular) (Six-base) MAPSORT of: pFN21AB8018.seq Check: 6859 from: 1 to: 6370
pFN21AB8018 6370 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3504 3504
Size: 6370
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4180 278
Size: 53 83 186 936 2644 2468
Fragments arranged by size:
2644 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3713 1937
Size: 1776 4594
Acc65I G'GTAC_C
Cuts at: 3691 3691
Size: 6370
AclI AA'CG_TT
Cuts at: 1563 4497 4870 1563
Size: 2934 373 3063
Fragments arranged by size:
3063 2934 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3026 4433 5817 6085 1632
Size: 1394 1407 1384 268 1917
Fragments arranged by size:
1917 1407 1394 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2870 820
Size: 197 1853 4320
Fragments arranged by size:
4320 1853 197
AflIII A'CryG_T
Cuts at: 1203 2707 5269 6246 1203
Size: 1504 2562 977 1327
Fragments arranged by size:
2562 1504 1327 977
AgeI A'CCGG_T
Cuts at: 3450 5201 3450
Size: 1751 4619
AhdI GACnn_n'nnGTC
Cuts at: 5099 5099
Size: 6370
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6370
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6370
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3612 3644 3612
Size: 32 6338
AlwNI CAG_nnn'CTG
Cuts at: 2125 2146 2606 4113 5685 2125
Size: 21 460 1507 1572 2810
Fragments arranged by size:
2810 1572 1507 460 21
ApaLI G'TGCA_C
Cuts at: 2214 4427 5583 2214
Size: 2213 1156 3001
Fragments arranged by size:
3001 2213 1156
ApoI r'AATT_y
Cuts at: 1493 2731 3157 3679 3905 4057 6166 1493
Size: 1238 426 522 226 152 2109 1697
Fragments arranged by size:
2109 1697 1238 522 426 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4924 160
Size: 872 3892 1606
Fragments arranged by size:
3892 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6370
AvaI C'yCGr_G
Cuts at: 1870 1943 3072 3354 3695 1870
Size: 73 1129 282 341 4545
Fragments arranged by size:
4545 1129 341 282 73
BamHI G'GATC_C
Cuts at: 3700 4067 3700
Size: 367 6003
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2390 3691 5146 618
Size: 325 217 627 603 1301 1455 1842
Fragments arranged by size:
1842 1455 1301 627 603 325 217
BanII G_rGCy'C
Cuts at: 1391 2030 2385 2428 3437 3689 1391
Size: 639 355 43 1009 252 4072
Fragments arranged by size:
4072 1009 639 355 252 43
BbeI G_GCGC'C
Cuts at: 2394 2394
Size: 6370
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5399
BbvCI CC'TCA_GC
Cuts at: 3595 3595
Size: 6370
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4561 4595 1903
Size: 34 2624 34 3678
Fragments arranged by size:
3678 2624 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2989 4262 5472 2989
Size: 1273 1210 3887
Fragments arranged by size:
3887 1273 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6370
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2399 2408 4981
Size: 107 122 71 631 605 727 9 2573
Cuts at: 4981 136
Size: 1525
Fragments arranged by size:
2573 1525 727 631 605 122 107 71
9
BglII A'GATC_T
Cuts at: 2747 3532 6365 2747
Size: 785 2833 2752
Fragments arranged by size:
2833 2752 785
BlpI GC'TnA_GC
Cuts at: 3787 3787
Size: 6370
Bme1580I G_kGCm'C
Cuts at: 1790 2218 4431 5587 1790
Size: 428 2213 1156 2573
Fragments arranged by size:
2573 2213 1156 428
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6370
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2968 4101 5059
Size: 416 53 83 399 24 1551 1133 958
Cuts at: 5059 442
Size: 1753
Fragments arranged by size:
1753 1551 1133 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6370
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3454 3456 3454
Size: 2 6368
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2168 2651 3442 5030 1239
Size: 929 483 791 1588 2579
Fragments arranged by size:
2579 1588 929 791 483
Bpu10I CC'TnA_GC
Cuts at: 3595 3595
Size: 6370
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4495 5360 5658 4495
Size: 865 298 5207
Fragments arranged by size:
5207 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5033 882
Size: 617 3534 2219
Fragments arranged by size:
3534 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2710 4161 493
Size: 711 1506 1451 2702
Fragments arranged by size:
2702 1506 1451 711
BsaBI GATnn'nnATC
Cuts at: 6364 6364
Size: 6370
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2391 4177 4559 5194
Size: 53 83 186 936 858 1786 382 635
Cuts at: 5194 275
Size: 1451
Fragments arranged by size:
1786 1451 936 858 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2852 3450 4803 5201 5475 5622 6033 2852
Size: 598 1353 398 274 147 411 3189
Fragments arranged by size:
3189 1353 598 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2174 2204 2893 2923 2941
Size: 30 165 30 771 30 689 30 18
Cuts at: 2941 2971 3440 3470 3612 3642 1178
Size: 30 469 30 142 30 3906
Fragments arranged by size:
3906 771 689 469 165 142 30 30
30 30 30 30 30 18
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2165 3036 3427 3583 1186
Size: 979 871 391 156 3973
Fragments arranged by size:
3973 979 871 391 156
BseYI C'CCAG_C
Cuts at: 2155 5573 6069 2155
Size: 3418 496 2456
Fragments arranged by size:
3418 2456 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5797
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3471 3820 4581 5609 1540
Size: 275 186 1470 349 761 1028 2301
Fragments arranged by size:
2301 1470 1028 761 349 275 186
BsiHKAI G_wGCw'C
Cuts at: 2218 2385 3437 4431 4516 5587 2218
Size: 167 1052 994 85 1071 3001
Fragments arranged by size:
3001 1071 1052 994 167 85
BsmI GAATG_Cn'
Cuts at: 3881 3974 3881
Size: 93 6277
BsmBI CGTCTCn'nnnn_
Cuts at: 3178 3178
Size: 6370
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2030 2218 2385 2428 3437 3689 4431
Size: 399 240 188 167 43 1009 252 742
Cuts at: 4431 4516 5587 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 1009 742 399 252 240 188
167 85 43
BspEI T'CCGG_A
Cuts at: 6033 6033
Size: 6370
BspHI T'CATG_A
Cuts at: 4259 5989 4259
Size: 1730 4640
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3504 3725 844
Size: 2660 221 3489
Fragments arranged by size:
3489 2660 221
BsrBI CCG'CTC
Cuts at: 1394 1823 3409 3475 4257 1394
Size: 429 1586 66 782 3507
Fragments arranged by size:
3507 1586 782 429 66
BsrDI GCAATG_nn'
Cuts at: 65 1248 3125 3198 4865 5039 65
Size: 1183 1877 73 1667 174 1396
Fragments arranged by size:
1877 1667 1396 1183 174 73
BsrFI r'CCGG_y
Cuts at: 1700 1810 3390 3450 5014 5201 1700
Size: 110 1580 60 1564 187 2869
Fragments arranged by size:
2869 1580 1564 187 110 60
BsrGI T'GTAC_A
Cuts at: 96 2679 2944 96
Size: 2583 265 3522
Fragments arranged by size:
3522 2583 265
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6370
BssSI C'ACGA_G
Cuts at: 4430 5442 4430
Size: 1012 5358
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2125 794
Size: 1331 5039
BstBI TT'CG_AA
Cuts at: 5174 5174
Size: 6370
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6370
BstXI CCAn_nnnn'nTGG
Cuts at: 2038 2038
Size: 6370
BstYI r'GATC_y
Cuts at: 1972 2602 2747 3031 3532 3700 4067 4452 4469
Size: 630 145 284 501 168 367 385 17
Cuts at: 4469 5921 6007 6019 6365 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 630 501 385 367 346 284
168 145 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3138 3138
Size: 6370
BtgI C'CryG_G
Cuts at: 513 1065 2004 3290 513
Size: 552 939 1286 3593
Fragments arranged by size:
3593 1286 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5631
Fragments arranged by size:
5631 683 56
BtsI GCAGTG_nn'
Cuts at: 3886 4680 4700 3886
Size: 794 20 5556
Fragments arranged by size:
5556 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6370
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2839 2874 3279 3314 529
Size: 35 2275 35 405 35 3585
Fragments arranged by size:
3585 2275 405 35 35 35
DraI TTT'AAA
Cuts at: 2565 3674 2565
Size: 1109 5261
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2316 2974 3357 5377 809
Size: 507 1000 658 383 2020 1802
Fragments arranged by size:
2020 1802 1000 658 507 383
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3817 4706 6335
Size: 54 1425 50 275 18 1987 889 1629
Cuts at: 6335 8
Size: 43
Fragments arranged by size:
1987 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3817 1537
Size: 275 2005 4090
Fragments arranged by size:
4090 2005 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3423 4300 1353
Size: 2070 877 3423
Fragments arranged by size:
3423 2070 877
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3039 4957 5331 5477 387
Size: 1064 138 1450 1918 374 146 1280
Fragments arranged by size:
1918 1450 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2383 3435 2383
Size: 1052 5318
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2168 2651 3026 3442 4433 5030 5817
Size: 393 536 483 375 416 991 597 787
Cuts at: 5817 6085 1239
Size: 268 1524
Fragments arranged by size:
1524 991 787 597 536 483 416 393
375 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2664 2664
Size: 6370
EcoO109I rG'GnC_Cy
Cuts at: 2143 2350 2401 2143
Size: 207 51 6112
Fragments arranged by size:
6112 207 51
EcoRI G'AATT_C
Cuts at: 3157 3679 4057 3157
Size: 522 378 5470
Fragments arranged by size:
5470 522 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2340 2533 2340
Size: 193 6177
FspI TGC'GCA
Cuts at: 4876 4876
Size: 6370
HaeII r_GCGC'y
Cuts at: 1678 2394 2881 3549 5517 1678
Size: 716 487 668 1968 2531
Fragments arranged by size:
2531 1968 716 668 487
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2091 2122 2123 2154 2830
Size: 32 135 32 519 31 1 31 676
Cuts at: 2830 2862 2941 2973 5011 5043 5085 5117 1373
Size: 32 79 32 2038 32 42 32 2626
Fragments arranged by size:
2626 2038 676 519 135 79 42 32
32 32 32 32 32 31 31 1
HincII GTy'rAC
Cuts at: 669 1657 1938 3663 3714 3960 669
Size: 988 281 1725 51 246 3079
Fragments arranged by size:
3079 1725 988 281 246 51
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6370
HpaI GTT'AAC
Cuts at: 3960 3960
Size: 6370
KasI G'GCGC_C
Cuts at: 2390 2390
Size: 6370
KpnI G_GTAC'C
Cuts at: 3695 3695
Size: 6370
MfeI C'AATT_G
Cuts at: 3969 3969
Size: 6370
MluI A'CGCG_T
Cuts at: 6246 6246
Size: 6370
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2040 2172 5484 5668 1305
Size: 735 132 3312 184 2007
Fragments arranged by size:
3312 2007 735 184 132
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4548
Fragments arranged by size:
4548 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2766 3257 4328 4687 4846 518
Size: 648 1600 491 1071 359 159 2042
Fragments arranged by size:
2042 1600 1071 648 491 359 159
MspA1I CmG'CkG
Cuts at: 1606 2122 2815 3639 3786 4463 5611 5856 1606
Size: 516 693 824 147 677 1148 245 2120
Fragments arranged by size:
2120 1148 824 693 677 516 245 147
NarI GG'CG_CC
Cuts at: 2391 2391
Size: 6370
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4879
Fragments arranged by size:
4879 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6370
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6370
NotI GC'GGCC_GC
Cuts at: 3817 3817
Size: 6370
NspI r_CATG'y
Cuts at: 1132 1229 2628 2680 3728 5273 1132
Size: 97 1399 52 1048 1545 2229
Fragments arranged by size:
2229 1545 1399 1048 97 52
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6370
PciI A'CATG_T
Cuts at: 5269 5269
Size: 6370
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3131 3281 1108
Size: 2023 150 4197
Fragments arranged by size:
4197 2023 150
PfoI T'CCnGG_A
Cuts at: 3027 3561 3027
Size: 534 5836
PmeI GTTT'AAAC
Cuts at: 3674 3674
Size: 6370
PmlI CAC'GTG
Cuts at: 2710 2710
Size: 6370
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3612 3644 4439 4471 5952 5984 3612
Size: 32 795 32 1481 32 3998
Fragments arranged by size:
3998 1481 795 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2143 2143
Size: 6370
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6370
PsiI TTA'TAA
Cuts at: 3940 3940
Size: 6370
PspXI vC'TCGA_Gb
Cuts at: 3354 3354
Size: 6370
PstI C_TGCA'G
Cuts at: 830 2130 2743 3722 830
Size: 1300 613 979 3478
Fragments arranged by size:
3478 1300 979 613
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6370
PvuII CAG'CTG
Cuts at: 2122 2815 3639 2122
Size: 693 824 4853
Fragments arranged by size:
4853 824 693
SacI G_AGCT'C
Cuts at: 2385 3437 2385
Size: 1052 5318
SalI G'TCGA_C
Cuts at: 1936 3712 1936
Size: 1776 4594
SbfI CC_TGCA'GG
Cuts at: 3722 3722
Size: 6370
ScaI AGT'ACT
Cuts at: 4618 6330 4618
Size: 1712 4658
SfcI C'TryA_G
Cuts at: 826 1045 2126 2499 2739 3718 4853 5534 5725
Size: 219 1081 373 240 979 1135 681 191
Cuts at: 5725 826
Size: 1471
Fragments arranged by size:
1471 1135 1081 979 681 373 240 219
191
SfoI GGC'GCC
Cuts at: 2392 2392
Size: 6370
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6370
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6370
SmaI CCC'GGG
Cuts at: 1872 3074 3697 1872
Size: 1202 623 4545
Fragments arranged by size:
4545 1202 623
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2870 3354 4474 5375 5637 820
Size: 197 926 927 484 1120 901 262 1553
Fragments arranged by size:
1553 1120 927 926 901 484 262 197
SnaBI TAC'GTA
Cuts at: 493 4161 493
Size: 3668 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6370
SphI G_CATG'C
Cuts at: 1132 3728 1132
Size: 2596 3774
SspI AAT'ATT
Cuts at: 5 52 4294 5
Size: 47 4242 2081
Fragments arranged by size:
4242 2081 47
StuI AGG'CCT
Cuts at: 1517 2670 1517
Size: 1153 5217
StyI C'CwwG_G
Cuts at: 513 1065 2004 2397 3809 513
Size: 552 939 393 1412 3074
Fragments arranged by size:
3074 1412 939 552 393
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3485 4397 4414 4567 4752 5197 325
Size: 1255 1905 912 17 153 185 445 1498
Fragments arranged by size:
1905 1498 1255 912 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2679 2944 4616
Size: 274 80 33 51 1443 702 265 1672
Cuts at: 4616 6328 96
Size: 1712 138
Fragments arranged by size:
1712 1672 1443 702 274 265 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6330 6362 6330
Size: 32 6338
Tth111I GACn'n_nGTC
Cuts at: 1316 2774 1316
Size: 1458 4912
XbaI T'CTAG_A
Cuts at: 3706 3706
Size: 6370
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2038 2404 2038
Size: 366 6004
XhoI C'TCGA_G
Cuts at: 1943 3354 1943
Size: 1411 4959
XmaI C'CCGG_G
Cuts at: 1870 3072 3695 1870
Size: 1202 623 4545
Fragments arranged by size:
4545 1202 623
XmnI GAAnn'nnTTC
Cuts at: 2778 3161 4499 2778
Size: 383 1338 4649
Fragments arranged by size:
4649 1338 383
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4178 276
Size: 53 83 186 936 2644 2468
Fragments arranged by size:
2644 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BfrBI BsiWI BstZ17I
DraIII EcoRV FseI FspAI NaeI NgoMIV NruI NsiI
PacI PspOMI PsrI RsrII SacII SanDI SapI SexAI
SfiI SrfI SwaI