Restriction map for
pFN21AB8009
(Circular) (Six-base) MAPSORT of: pFN21AB8009.seq Check: 3942 from: 1 to: 5854
pFN21AB8009 5854 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2084 3664 278
Size: 53 83 186 936 548 1580 2468
Fragments arranged by size:
2468 1580 936 548 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3197 1937
Size: 1260 4594
Acc65I G'GTAC_C
Cuts at: 2884 3175 2884
Size: 291 5563
AclI AA'CG_TT
Cuts at: 1563 3981 4354 1563
Size: 2418 373 3063
Fragments arranged by size:
3063 2418 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2520 2613 2912 2990 3917 5301 5569 1632
Size: 888 93 299 78 927 1384 268 1917
Fragments arranged by size:
1917 1384 927 888 299 268 93 78
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5657
AflIII A'CryG_T
Cuts at: 1203 4753 5730 1203
Size: 3550 977 1327
Fragments arranged by size:
3550 1327 977
AgeI A'CCGG_T
Cuts at: 4685 4685
Size: 5854
AhdI GACnn_n'nnGTC
Cuts at: 4583 4583
Size: 5854
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5854
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2817 3144 1184
Size: 1633 327 3894
Fragments arranged by size:
3894 1633 327
AlwNI CAG_nnn'CTG
Cuts at: 3597 5169 3597
Size: 1572 4282
ApaLI G'TGCA_C
Cuts at: 2457 3911 5067 2457
Size: 1454 1156 3244
Fragments arranged by size:
3244 1454 1156
ApoI r'AATT_y
Cuts at: 1493 2572 2744 2913 3148 3163 3389 3541 5650
Size: 1079 172 169 235 15 226 152 2109
Cuts at: 5650 1493
Size: 1697
Fragments arranged by size:
2109 1697 1079 235 226 172 169 152
15
AseI AT'TA_AT
Cuts at: 160 1032 4408 160
Size: 872 3376 1606
Fragments arranged by size:
3376 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5854
AvaI C'yCGr_G
Cuts at: 1870 1943 3179 1870
Size: 73 1236 4545
Fragments arranged by size:
4545 1236 73
BamHI G'GATC_C
Cuts at: 3184 3551 3184
Size: 367 5487
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2884 3175 4630 618
Size: 325 217 627 1097 291 1455 1842
Fragments arranged by size:
1842 1455 1097 627 325 291 217
BanII G_rGCy'C
Cuts at: 1391 2947 3173 1391
Size: 1556 226 4072
Fragments arranged by size:
4072 1556 226
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2150 2472 928
Size: 971 251 322 4310
Fragments arranged by size:
4310 971 322 251
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4045 4079 1903
Size: 34 50 34 2024 34 3678
Fragments arranged by size:
3678 2024 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2995 3746 4956 2995
Size: 751 1210 3893
Fragments arranged by size:
3893 1210 751
BclI T'GATC_A
Cuts at: 1548 2824 1548
Size: 1276 4578
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4465 136
Size: 107 122 71 631 605 2793 1525
Fragments arranged by size:
2793 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5849 5849
Size: 5854
BlpI GC'TnA_GC
Cuts at: 3271 3271
Size: 5854
Bme1580I G_kGCm'C
Cuts at: 1790 2461 3915 5071 1790
Size: 671 1454 1156 2573
Fragments arranged by size:
2573 1454 1156 671
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5854
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3585 4543 442
Size: 416 53 83 399 24 2168 958 1753
Fragments arranged by size:
2168 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5854
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2709 2709
Size: 5854
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2214 2704 4514 1239
Size: 975 490 1810 2579
Fragments arranged by size:
2579 1810 975 490
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2717 3979 4844 5142 2717
Size: 1262 865 298 3429
Fragments arranged by size:
3429 1262 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4517 882
Size: 617 3018 2219
Fragments arranged by size:
3018 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2068 3645 493
Size: 711 864 1577 2702
Fragments arranged by size:
2702 1577 864 711
BsaBI GATnn'nnATC
Cuts at: 5848 5848
Size: 5854
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2081 3661 4043 4678
Size: 53 83 186 936 548 1580 382 635
Cuts at: 4678 275
Size: 1451
Fragments arranged by size:
1580 1451 936 635 548 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4287 4685 4959 5106 5517 4287
Size: 398 274 147 411 4624
Fragments arranged by size:
4624 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2187 2217 1178
Size: 30 165 30 784 30 4815
Fragments arranged by size:
4815 784 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5854
BseYI C'CCAG_C
Cuts at: 5057 5553 5057
Size: 496 5358
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2075 2271 1163
Size: 573 339 196 4746
Fragments arranged by size:
4746 573 339 196
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2049 3304 4065 5093 1540
Size: 275 186 48 1255 761 1028 2301
Fragments arranged by size:
2301 1255 1028 761 275 186 48
BsiHKAI G_wGCw'C
Cuts at: 2461 2806 3915 4000 5071 2461
Size: 345 1109 85 1071 3244
Fragments arranged by size:
3244 1109 1071 345 85
BsmI GAATG_Cn'
Cuts at: 2514 3021 3365 3458 2514
Size: 507 344 93 4910
Fragments arranged by size:
4910 507 344 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2072 2072
Size: 5854
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2461 2806 2947 3173 3915 4000 5071
Size: 399 671 345 141 226 742 85 1071
Cuts at: 5071 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 671 399 345 226 141
85
BspEI T'CCGG_A
Cuts at: 5517 5517
Size: 5854
BspHI T'CATG_A
Cuts at: 2251 3033 3743 5473 2251
Size: 782 710 1730 2632
Fragments arranged by size:
2632 1730 782 710
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2896 3209 844
Size: 2052 313 3489
Fragments arranged by size:
3489 2052 313
BsrBI CCG'CTC
Cuts at: 1394 1823 3741 1394
Size: 429 1918 3507
Fragments arranged by size:
3507 1918 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4349 4523 65
Size: 1183 3101 174 1396
Fragments arranged by size:
3101 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2044 4498 4685 1700
Size: 110 234 2454 187 2869
Fragments arranged by size:
2869 2454 234 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5854
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5854
BssSI C'ACGA_G
Cuts at: 2940 3914 4926 2940
Size: 974 1012 3868
Fragments arranged by size:
3868 1012 974
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5854
BstBI TT'CG_AA
Cuts at: 4658 4658
Size: 5854
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5854
BstXI CCAn_nnnn'nTGG
Cuts at: 3140 3140
Size: 5854
BstYI r'GATC_y
Cuts at: 1972 3184 3551 3936 3953 5405 5491 5503 5849
Size: 1212 367 385 17 1452 86 12 346
Cuts at: 5849 1972
Size: 1977
Fragments arranged by size:
1977 1452 1212 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2584 513
Size: 552 1519 3783
Fragments arranged by size:
3783 1519 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5115
Fragments arranged by size:
5115 683 56
BtsI GCAGTG_nn'
Cuts at: 3128 3370 4164 4184 3128
Size: 242 794 20 4798
Fragments arranged by size:
4798 794 242 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5854
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5819
DraI TTT'AAA
Cuts at: 3158 3158
Size: 5854
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4861 809
Size: 507 3545 1802
Fragments arranged by size:
3545 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2046 2441 3301
Size: 54 1425 50 275 18 216 395 860
Cuts at: 3301 4190 5819 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 860 395 275 216 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2046 3301 1537
Size: 275 234 1255 4090
Fragments arranged by size:
4090 1255 275 234
EarI CTCTTCn'nnn_
Cuts at: 1353 2260 2504 2987 3035 3784 1353
Size: 907 244 483 48 749 3423
Fragments arranged by size:
3423 907 749 483 244 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4441 4815 4961 387
Size: 1064 138 2852 374 146 1280
Fragments arranged by size:
2852 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2214 2520 2613 2704 2912 2990 3917
Size: 393 582 306 93 91 208 78 927
Cuts at: 3917 4514 5301 5569 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 927 787 597 582 393 306 268
208 93 91 78
EcoRI G'AATT_C
Cuts at: 3163 3541 3163
Size: 378 5476
EcoRV GAT'ATC
Cuts at: 2152 2152
Size: 5854
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3046 3104 3046
Size: 58 5796
FspI TGC'GCA
Cuts at: 4360 4360
Size: 5854
HaeII r_GCGC'y
Cuts at: 1678 5001 1678
Size: 3323 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2071 2103 4495 4527 4569
Size: 32 135 32 499 32 2392 32 42
Cuts at: 4569 4601 1373
Size: 32 2626
Fragments arranged by size:
2626 2392 499 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3198 3444 669
Size: 988 281 1260 246 3079
Fragments arranged by size:
3079 1260 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5854
HpaI GTT'AAC
Cuts at: 3444 3444
Size: 5854
KpnI G_GTAC'C
Cuts at: 2888 3179 2888
Size: 291 5563
MfeI C'AATT_G
Cuts at: 3453 3453
Size: 5854
MluI A'CGCG_T
Cuts at: 5730 5730
Size: 5854
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2153 3078 4968 5152 1305
Size: 848 925 1890 184 2007
Fragments arranged by size:
2007 1890 925 848 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4032
Fragments arranged by size:
4032 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2799 3812 4171 4330 518
Size: 648 1633 1013 359 159 2042
Fragments arranged by size:
2042 1633 1013 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2989 3270 3947 5095 5340 1606
Size: 1383 281 677 1148 245 2120
Fragments arranged by size:
2120 1383 1148 677 281 245
NaeI GCC'GGC
Cuts at: 2046 2046
Size: 5854
NcoI C'CATG_G
Cuts at: 513 1065 2584 513
Size: 552 1519 3783
Fragments arranged by size:
3783 1519 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5854
NgoMIV G'CCGG_C
Cuts at: 2044 2044
Size: 5854
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5854
NotI GC'GGCC_GC
Cuts at: 3301 3301
Size: 5854
NspI r_CATG'y
Cuts at: 1132 1229 2534 3212 4757 1132
Size: 97 1305 678 1545 2229
Fragments arranged by size:
2229 1545 1305 678 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5854
PciI A'CATG_T
Cuts at: 4753 4753
Size: 5854
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5854
PfoI T'CCnGG_A
Cuts at: 2716 2716
Size: 5854
PmeI GTTT'AAAC
Cuts at: 3158 3158
Size: 5854
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3923 3955 5436 5468 3923
Size: 32 1481 32 4309
Fragments arranged by size:
4309 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5854
PsiI TTA'TAA
Cuts at: 3424 3424
Size: 5854
PstI C_TGCA'G
Cuts at: 830 2911 3206 830
Size: 2081 295 3478
Fragments arranged by size:
3478 2081 295
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5854
PvuII CAG'CTG
Cuts at: 2989 2989
Size: 5854
SalI G'TCGA_C
Cuts at: 1936 3196 1936
Size: 1260 4594
SapI GCTCTTCn'nnn_
Cuts at: 3035 3035
Size: 5854
SbfI CC_TGCA'GG
Cuts at: 3206 3206
Size: 5854
ScaI AGT'ACT
Cuts at: 4102 5814 4102
Size: 1712 4142
SfcI C'TryA_G
Cuts at: 826 1045 2470 2907 3202 4337 5018 5209 826
Size: 219 1425 437 295 1135 681 191 1471
Fragments arranged by size:
1471 1425 1135 681 437 295 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5854
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5854
SmaI CCC'GGG
Cuts at: 1872 3181 1872
Size: 1309 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2696 3958 4859 5121 820
Size: 197 926 753 1262 901 262 1553
Fragments arranged by size:
1553 1262 926 901 753 262 197
SnaBI TAC'GTA
Cuts at: 493 3645 493
Size: 3152 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5854
SphI G_CATG'C
Cuts at: 1132 2534 3212 1132
Size: 1402 678 3774
Fragments arranged by size:
3774 1402 678
SspI AAT'ATT
Cuts at: 5 52 2651 3778 5
Size: 47 2599 1127 2081
Fragments arranged by size:
2599 2081 1127 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5854
StyI C'CwwG_G
Cuts at: 513 1065 2584 3293 513
Size: 552 1519 709 3074
Fragments arranged by size:
3074 1519 709 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3881 3898 4051 4236 4681 325
Size: 1255 2301 17 153 185 445 1498
Fragments arranged by size:
2301 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4100 5812 96
Size: 274 80 33 51 1443 2123 1712 138
Fragments arranged by size:
2123 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5814 5846 5814
Size: 32 5822
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5854
XbaI T'CTAG_A
Cuts at: 3190 3190
Size: 5854
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5854
XmaI C'CCGG_G
Cuts at: 1870 3179 1870
Size: 1309 4545
XmnI GAAnn'nnTTC
Cuts at: 2709 3983 2709
Size: 1274 4580
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2082 3662 276
Size: 53 83 186 936 548 1580 2468
Fragments arranged by size:
2468 1580 936 548 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I FseI FspAI KasI NarI NruI NsiI
PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI