Restriction map for
pFN21AB8007
(Circular) (Six-base) MAPSORT of: pFN21AB8007.seq Check: 567 from: 1 to: 7471
pFN21AB8007 7471 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5281 278
Size: 53 83 186 936 3745 2468
Fragments arranged by size:
3745 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4814 1937
Size: 2877 4594
Acc65I G'GTAC_C
Cuts at: 4792 4792
Size: 7471
AclI AA'CG_TT
Cuts at: 1563 5598 5971 1563
Size: 4035 373 3063
Fragments arranged by size:
4035 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2508 2817 2940 4241 4346 5534 6918 7186
Size: 876 309 123 1301 105 1188 1384 268
Cuts at: 7186 1632
Size: 1917
Fragments arranged by size:
1917 1384 1301 1188 876 309 268 123
105
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7274
AflIII A'CryG_T
Cuts at: 1203 4670 6370 7347 1203
Size: 3467 1700 977 1327
Fragments arranged by size:
3467 1700 1327 977
AgeI A'CCGG_T
Cuts at: 6302 6302
Size: 7471
AhdI GACnn_n'nnGTC
Cuts at: 6200 6200
Size: 7471
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7471
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7471
AlwNI CAG_nnn'CTG
Cuts at: 5214 6786 5214
Size: 1572 5899
ApaLI G'TGCA_C
Cuts at: 5528 6684 5528
Size: 1156 6315
ApoI r'AATT_y
Cuts at: 1493 2240 3259 4109 4780 5006 5158 7267 1493
Size: 747 1019 850 671 226 152 2109 1697
Fragments arranged by size:
2109 1697 1019 850 747 671 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3951 6025 160
Size: 872 2919 2074 1606
Fragments arranged by size:
2919 2074 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7471
AvaI C'yCGr_G
Cuts at: 1870 1943 4302 4796 1870
Size: 73 2359 494 4545
Fragments arranged by size:
4545 2359 494 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2772 2805 2772
Size: 33 7438
BamHI G'GATC_C
Cuts at: 3013 4801 5168 3013
Size: 1788 367 5316
Fragments arranged by size:
5316 1788 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4792 6247 618
Size: 325 217 627 3005 1455 1842
Fragments arranged by size:
3005 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4596 4790 1391
Size: 3205 194 4072
Fragments arranged by size:
4072 3205 194
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6500
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5662 5696 1903
Size: 34 3725 34 3678
Fragments arranged by size:
3725 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5363 6573 5363
Size: 1210 6261
BclI T'GATC_A
Cuts at: 1548 3550 1548
Size: 2002 5469
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2546 6082 136
Size: 107 122 71 631 605 874 3536 1525
Fragments arranged by size:
3536 1525 874 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7466 7466
Size: 7471
BlpI GC'TnA_GC
Cuts at: 4590 4888 4590
Size: 298 7173
Bme1580I G_kGCm'C
Cuts at: 1790 5532 6688 1790
Size: 3742 1156 2573
Fragments arranged by size:
3742 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7471
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3445 5202 6160
Size: 416 53 83 399 24 2028 1757 958
Cuts at: 6160 442
Size: 1753
Fragments arranged by size:
2028 1757 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7471
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2769 2832 3141 6131 1239
Size: 1530 63 309 2990 2579
Fragments arranged by size:
2990 2579 1530 309 63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3456 3799 5596 6461 6759 3456
Size: 343 1797 865 298 4168
Fragments arranged by size:
4168 1797 865 343 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3133 6134 882
Size: 617 1634 3001 2219
Fragments arranged by size:
3001 2219 1634 617
BsaAI yAC'GTr
Cuts at: 493 1204 5262 493
Size: 711 4058 2702
Fragments arranged by size:
4058 2702 711
BsaBI GATnn'nnATC
Cuts at: 7465 7465
Size: 7471
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5278 5660 6295 275
Size: 53 83 186 936 3745 382 635 1451
Fragments arranged by size:
3745 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5904 6302 6576 6723 7134 5904
Size: 398 274 147 411 6241
Fragments arranged by size:
6241 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2674 2704 2767 2797 1178
Size: 30 165 30 1271 30 63 30 5852
Fragments arranged by size:
5852 1271 165 63 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3796 3822 4268 4320 1186
Size: 2610 26 446 52 4337
Fragments arranged by size:
4337 2610 446 52 26
BseYI C'CCAG_C
Cuts at: 3387 3507 6674 7170 3387
Size: 120 3167 496 3688
Fragments arranged by size:
3688 3167 496 120
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2925 1163
Size: 573 1189 5709
Fragments arranged by size:
5709 1189 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4921 5682 6710 1540
Size: 275 186 2920 761 1028 2301
Fragments arranged by size:
2920 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4596 5532 5617 6688 4596
Size: 936 85 1071 5379
Fragments arranged by size:
5379 1071 936 85
BsmI GAATG_Cn'
Cuts at: 3043 4539 4982 5075 3043
Size: 1496 443 93 5439
Fragments arranged by size:
5439 1496 443 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2661 2661
Size: 7471
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4596 4790 5532 5617 6688 1391
Size: 399 2806 194 742 85 1071 2174
Fragments arranged by size:
2806 2174 1071 742 399 194 85
BspEI T'CCGG_A
Cuts at: 7134 7134
Size: 7471
BspHI T'CATG_A
Cuts at: 5360 7090 5360
Size: 1730 5741
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4571 4826 844
Size: 3727 255 3489
Fragments arranged by size:
3727 3489 255
BsrBI CCG'CTC
Cuts at: 1394 1823 5358 1394
Size: 429 3535 3507
Fragments arranged by size:
3535 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3595 5966 6140 65
Size: 1183 2347 2371 174 1396
Fragments arranged by size:
2371 2347 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6115 6302 1700
Size: 110 4305 187 2869
Fragments arranged by size:
4305 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7471
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7471
BssSI C'ACGA_G
Cuts at: 3525 5531 6543 3525
Size: 2006 1012 4453
Fragments arranged by size:
4453 2006 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7471
BstBI TT'CG_AA
Cuts at: 6275 6275
Size: 7471
BstEII G'GTnAC_C
Cuts at: 1184 4653 1184
Size: 3469 4002
BstXI CCAn_nnnn'nTGG
Cuts at: 3066 3387 3507 4243 3066
Size: 321 120 736 6294
Fragments arranged by size:
6294 736 321 120
BstYI r'GATC_y
Cuts at: 1972 2842 3013 3082 3460 3580 4687 4801 5168
Size: 870 171 69 378 120 1107 114 367
Cuts at: 5168 5553 5570 7022 7108 7120 7466 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1107 870 385 378 367 346
171 120 114 86 69 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3243 3999 513
Size: 552 939 1239 756 3985
Fragments arranged by size:
3985 1239 939 756 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2243 487
Size: 683 56 1017 5715
Fragments arranged by size:
5715 1017 683 56
BtsI GCAGTG_nn'
Cuts at: 2010 4987 5781 5801 2010
Size: 2977 794 20 3680
Fragments arranged by size:
3680 2977 794 20
ClaI AT'CG_AT
Cuts at: 1554 4026 1554
Size: 2472 4999
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7436
DraI TTT'AAA
Cuts at: 2268 4775 2268
Size: 2507 4964
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6478 809
Size: 507 5162 1802
Fragments arranged by size:
5162 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 4918 5807
Size: 54 1425 50 275 18 177 2911 889
Cuts at: 5807 7436 8
Size: 1629 43
Fragments arranged by size:
2911 1629 1425 889 275 177 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4918 1537
Size: 275 3106 4090
Fragments arranged by size:
4090 3106 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3166 3184 4365 4740 5401 1353
Size: 1813 18 1181 375 661 3423
Fragments arranged by size:
3423 1813 1181 661 375 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6058 6432 6578 387
Size: 1064 138 4469 374 146 1280
Fragments arranged by size:
4469 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4594 4594
Size: 7471
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2508 2769 2817 2832 2940 3141 4241
Size: 393 876 261 48 15 108 201 1100
Cuts at: 4241 4346 5534 6131 6918 7186 1239
Size: 105 1188 597 787 268 1524
Fragments arranged by size:
1524 1188 1100 876 787 597 393 268
261 201 108 105 48 15
EcoNI CCTnn'n_nnAGG
Cuts at: 4578 4578
Size: 7471
EcoRI G'AATT_C
Cuts at: 4780 5158 4780
Size: 378 7093
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3836 4349 3836
Size: 513 6958
FspI TGC'GCA
Cuts at: 5977 5977
Size: 7471
HaeII r_GCGC'y
Cuts at: 1678 3790 6618 1678
Size: 2112 2828 2531
Fragments arranged by size:
2828 2531 2112
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2753 2785 4079 4111 6112
Size: 32 135 32 1181 32 1294 32 2001
Cuts at: 6112 6144 6186 6218 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2001 1294 1181 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4183 4426 4815 5061 669
Size: 988 281 2245 243 389 246 3079
Fragments arranged by size:
3079 2245 988 389 281 246 243
HindIII A'AGCT_T
Cuts at: 748 3653 4326 748
Size: 2905 673 3893
Fragments arranged by size:
3893 2905 673
HpaI GTT'AAC
Cuts at: 5061 5061
Size: 7471
KpnI G_GTAC'C
Cuts at: 4796 4796
Size: 7471
MfeI C'AATT_G
Cuts at: 3290 5070 3290
Size: 1780 5691
MluI A'CGCG_T
Cuts at: 7347 7347
Size: 7471
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2495 3772 6585 6769 1305
Size: 1190 1277 2813 184 2007
Fragments arranged by size:
2813 2007 1277 1190 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 5472
Fragments arranged by size:
5472 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2511 3064 5429 5788 5947 518
Size: 648 1345 553 2365 359 159 2042
Fragments arranged by size:
2365 2042 1345 648 553 359 159
MspA1I CmG'CkG
Cuts at: 1606 3235 3283 4887 5564 6712 6957 1606
Size: 1629 48 1604 677 1148 245 2120
Fragments arranged by size:
2120 1629 1604 1148 677 245 48
NcoI C'CATG_G
Cuts at: 513 1065 2004 3243 3999 513
Size: 552 939 1239 756 3985
Fragments arranged by size:
3985 1239 939 756 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7471
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7471
NotI GC'GGCC_GC
Cuts at: 4918 4918
Size: 7471
NspI r_CATG'y
Cuts at: 1132 1229 4417 4674 4829 6374 1132
Size: 97 3188 257 155 1545 2229
Fragments arranged by size:
3188 2229 1545 257 155 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7471
PciI A'CATG_T
Cuts at: 4670 6370 4670
Size: 1700 5771
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7471
PmeI GTTT'AAAC
Cuts at: 4775 4775
Size: 7471
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5540 5572 7053 7085 5540
Size: 32 1481 32 5926
Fragments arranged by size:
5926 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7471
PsiI TTA'TAA
Cuts at: 4116 5041 4116
Size: 925 6546
PstI C_TGCA'G
Cuts at: 830 2462 3355 3930 4823 830
Size: 1632 893 575 893 3478
Fragments arranged by size:
3478 1632 893 893 575
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7471
SacI G_AGCT'C
Cuts at: 4596 4596
Size: 7471
SalI G'TCGA_C
Cuts at: 1936 4813 1936
Size: 2877 4594
SbfI CC_TGCA'GG
Cuts at: 4823 4823
Size: 7471
ScaI AGT'ACT
Cuts at: 2830 4376 5719 7431 2830
Size: 1546 1343 1712 2870
Fragments arranged by size:
2870 1712 1546 1343
SfcI C'TryA_G
Cuts at: 826 1045 2458 3351 3926 4370 4819 5954 6635
Size: 219 1413 893 575 444 449 1135 681
Cuts at: 6635 6826 826
Size: 191 1471
Fragments arranged by size:
1471 1413 1135 893 681 575 449 444
219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7471
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7471
SmaI CCC'GGG
Cuts at: 1872 4798 1872
Size: 2926 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3471 3778 4302 5575 6476 6738
Size: 197 926 1528 307 524 1273 901 262
Cuts at: 6738 820
Size: 1553
Fragments arranged by size:
1553 1528 1273 926 901 524 307 262
197
SnaBI TAC'GTA
Cuts at: 493 5262 493
Size: 4769 2702
SpeI A'CTAG_T
Cuts at: 152 2033 2825 152
Size: 1881 792 4798
Fragments arranged by size:
4798 1881 792
SphI G_CATG'C
Cuts at: 1132 4417 4829 1132
Size: 3285 412 3774
Fragments arranged by size:
3774 3285 412
SspI AAT'ATT
Cuts at: 5 52 3801 4240 5395 5
Size: 47 3749 439 1155 2081
Fragments arranged by size:
3749 2081 1155 439 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7471
StyI C'CwwG_G
Cuts at: 513 1065 2004 2021 2370 3243 3999 4910 513
Size: 552 939 17 349 873 756 911 3074
Fragments arranged by size:
3074 939 911 873 756 552 349 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2994 5498 5515 5668 5853 6298 325
Size: 1255 1414 2504 17 153 185 445 1498
Fragments arranged by size:
2504 1498 1414 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2828 4170 4309
Size: 274 80 33 51 1443 851 1342 139
Cuts at: 4309 4374 5717 7429 96
Size: 65 1343 1712 138
Fragments arranged by size:
1712 1443 1343 1342 851 274 139 138
80 65 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2767 2799 7431 7463 2767
Size: 32 4632 32 2775
Fragments arranged by size:
4632 2775 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7471
XbaI T'CTAG_A
Cuts at: 2957 4807 2957
Size: 1850 5621
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2908 2908
Size: 7471
XhoI C'TCGA_G
Cuts at: 1943 4302 1943
Size: 2359 5112
XmaI C'CCGG_G
Cuts at: 1870 4796 1870
Size: 2926 4545
XmnI GAAnn'nnTTC
Cuts at: 2108 2889 2965 4353 4728 5600 2108
Size: 781 76 1388 375 872 3979
Fragments arranged by size:
3979 1388 872 781 375 76
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5279 276
Size: 53 83 186 936 3745 2468
Fragments arranged by size:
3745 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI