Restriction map for
pFN21AB8005
(Circular) (Six-base) MAPSORT of: pFN21AB8005.seq Check: 9322 from: 1 to: 6949
pFN21AB8005 6949 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4759 278
Size: 53 83 186 936 3223 2468
Fragments arranged by size:
3223 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4292 1937
Size: 2355 4594
Acc65I G'GTAC_C
Cuts at: 4270 4270
Size: 6949
AclI AA'CG_TT
Cuts at: 1563 5076 5449 1563
Size: 3513 373 3063
Fragments arranged by size:
3513 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2396 2658 3866 4004 5012 6396 6664 1632
Size: 764 262 1208 138 1008 1384 268 1917
Fragments arranged by size:
1917 1384 1208 1008 764 268 262 138
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6752
AflIII A'CryG_T
Cuts at: 1203 2660 4229 5848 6825 1203
Size: 1457 1569 1619 977 1327
Fragments arranged by size:
1619 1569 1457 1327 977
AgeI A'CCGG_T
Cuts at: 5780 5780
Size: 6949
AhdI GACnn_n'nnGTC
Cuts at: 5678 5678
Size: 6949
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6949
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3196 4095 1184
Size: 2012 899 4038
Fragments arranged by size:
4038 2012 899
AlwNI CAG_nnn'CTG
Cuts at: 2159 4692 6264 2159
Size: 2533 1572 2844
Fragments arranged by size:
2844 2533 1572
ApaI G_GGCC'C
Cuts at: 2037 2037
Size: 6949
ApaLI G'TGCA_C
Cuts at: 5006 6162 5006
Size: 1156 5793
ApoI r'AATT_y
Cuts at: 1493 3135 3278 4258 4484 4636 6745 1493
Size: 1642 143 980 226 152 2109 1697
Fragments arranged by size:
2109 1697 1642 980 226 152 143
AseI AT'TA_AT
Cuts at: 160 1032 3269 5503 160
Size: 872 2237 2234 1606
Fragments arranged by size:
2237 2234 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6949
AvaI C'yCGr_G
Cuts at: 1870 1943 4041 4087 4274 1870
Size: 73 2098 46 187 4545
Fragments arranged by size:
4545 2098 187 73 46
BamHI G'GATC_C
Cuts at: 4034 4279 4646 4034
Size: 245 367 6337
Fragments arranged by size:
6337 367 245
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4270 5725 618
Size: 325 217 627 2483 1455 1842
Fragments arranged by size:
2483 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2037 3876 4268 1391
Size: 646 1839 392 4072
Fragments arranged by size:
4072 1839 646 392
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2291 2402 2664 3854 928
Size: 971 392 111 262 1190 4023
Fragments arranged by size:
4023 1190 971 392 262 111
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5140 5174 1903
Size: 34 3203 34 3678
Fragments arranged by size:
3678 3203 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3603 3725 4841 6051 3603
Size: 122 1116 1210 4501
Fragments arranged by size:
4501 1210 1116 122
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6949
BfrBI ATG'CAT
Cuts at: 3668 3668
Size: 6949
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5560 136
Size: 107 122 71 631 605 3888 1525
Fragments arranged by size:
3888 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3253 6944 3253
Size: 3691 3258
BlpI GC'TnA_GC
Cuts at: 4366 4366
Size: 6949
Bme1580I G_kGCm'C
Cuts at: 1790 2037 2859 5010 6166 1790
Size: 247 822 2151 1156 2573
Fragments arranged by size:
2573 2151 1156 822 247
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6949
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4680 5638 442
Size: 416 53 83 399 24 3263 958 1753
Fragments arranged by size:
3263 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6949
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2739 3787 5609 1239
Size: 1500 1048 1822 2579
Fragments arranged by size:
2579 1822 1500 1048
Bpu10I CC'TnA_GC
Cuts at: 2115 2115
Size: 6949
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3635 5074 5939 6237 3635
Size: 1439 865 298 4347
Fragments arranged by size:
4347 1439 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2415 3108 3238 5612 882
Size: 617 916 693 130 2374 2219
Fragments arranged by size:
2374 2219 916 693 617 130
BsaAI yAC'GTr
Cuts at: 493 1204 4740 493
Size: 711 3536 2702
Fragments arranged by size:
3536 2702 711
BsaBI GATnn'nnATC
Cuts at: 3585 6943 3585
Size: 3358 3591
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4756 5138 5773 275
Size: 53 83 186 936 3223 382 635 1451
Fragments arranged by size:
3223 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3433 3583 5382 5780 6054 6201 6612 3433
Size: 150 1799 398 274 147 411 3770
Fragments arranged by size:
3770 1799 411 398 274 150 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2430 2460 1178
Size: 30 165 30 1027 30 5667
Fragments arranged by size:
5667 1027 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2710 2742 1186
Size: 1524 32 5393
Fragments arranged by size:
5393 1524 32
BseYI C'CCAG_C
Cuts at: 2490 6152 6648 2490
Size: 3662 496 2791
Fragments arranged by size:
3662 2791 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4105 1163
Size: 573 2369 4007
Fragments arranged by size:
4007 2369 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2576 4399 5160 6188 1540
Size: 275 186 575 1823 761 1028 2301
Fragments arranged by size:
2301 1823 1028 761 575 275 186
BsiHKAI G_wGCw'C
Cuts at: 2837 3876 5010 5095 6166 2837
Size: 1039 1134 85 1071 3620
Fragments arranged by size:
3620 1134 1071 1039 85
BsmI GAATG_Cn'
Cuts at: 3092 4460 4553 3092
Size: 1368 93 5488
Fragments arranged by size:
5488 1368 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2037 2837 2859 3876 4268 5010 5095
Size: 399 247 800 22 1017 392 742 85
Cuts at: 5095 6166 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 1017 800 742 399 392 247
85 22
BspEI T'CCGG_A
Cuts at: 3433 3583 6612 3433
Size: 150 3029 3770
Fragments arranged by size:
3770 3029 150
BspHI T'CATG_A
Cuts at: 3234 4838 6568 3234
Size: 1604 1730 3615
Fragments arranged by size:
3615 1730 1604
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3164 4140 4304 844
Size: 2320 976 164 3489
Fragments arranged by size:
3489 2320 976 164
BsrBI CCG'CTC
Cuts at: 1394 1823 4836 1394
Size: 429 3013 3507
Fragments arranged by size:
3507 3013 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2147 2561 2897 3178 3401 3518 4067
Size: 1183 899 414 336 281 223 117 549
Cuts at: 4067 5444 5618 65
Size: 1377 174 1396
Fragments arranged by size:
1396 1377 1183 899 549 414 336 281
223 174 117
BsrFI r'CCGG_y
Cuts at: 1700 1810 2575 5593 5780 1700
Size: 110 765 3018 187 2869
Fragments arranged by size:
3018 2869 765 187 110
BsrGI T'GTAC_A
Cuts at: 96 3941 96
Size: 3845 3104
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6949
BssSI C'ACGA_G
Cuts at: 5009 6021 5009
Size: 1012 5937
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6949
BstBI TT'CG_AA
Cuts at: 5753 5753
Size: 6949
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6949
BstXI CCAn_nnnn'nTGG
Cuts at: 2490 3461 2490
Size: 971 5978
BstYI r'GATC_y
Cuts at: 1972 2132 2256 2677 2863 2916 2983 3253 3580
Size: 160 124 421 186 53 67 270 327
Cuts at: 3580 3586 4034 4279 4646 5031 5048 6500 6586
Size: 6 448 245 367 385 17 1452 86
Cuts at: 6586 6598 6944 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 448 421 385 367 346 327
270 245 186 160 124 86 67 53
17 12 6
Bsu36I CC'TnA_GG
Cuts at: 3168 3168
Size: 6949
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6397
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6210
Fragments arranged by size:
6210 683 56
BtsI GCAGTG_nn'
Cuts at: 4465 5259 5279 4465
Size: 794 20 6135
Fragments arranged by size:
6135 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6949
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6914
DraI TTT'AAA
Cuts at: 3018 4253 3018
Size: 1235 5714
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5956 809
Size: 507 4640 1802
Fragments arranged by size:
4640 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4396 5285 6914
Size: 54 1425 50 275 18 2566 889 1629
Cuts at: 6914 8
Size: 43
Fragments arranged by size:
2566 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4396 1537
Size: 275 2584 4090
Fragments arranged by size:
4090 2584 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2880 3198 3377 3993 4879 1353
Size: 1527 318 179 616 886 3423
Fragments arranged by size:
3423 1527 886 616 318 179
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5536 5910 6056 387
Size: 1064 138 3947 374 146 1280
Fragments arranged by size:
3947 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3874 3874
Size: 6949
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2396 2658 2739 3787 3866 4004 5012
Size: 393 764 262 81 1048 79 138 1008
Cuts at: 5012 5609 6396 6664 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1048 1008 787 764 597 393 268
262 138 81 79
EcoNI CCTnn'n_nnAGG
Cuts at: 3171 3171
Size: 6949
EcoRI G'AATT_C
Cuts at: 4258 4636 4258
Size: 378 6571
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3314 3314
Size: 6949
FspI TGC'GCA
Cuts at: 5455 5455
Size: 6949
HaeII r_GCGC'y
Cuts at: 1678 2566 6096 1678
Size: 888 3530 2531
Fragments arranged by size:
3530 2531 888
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2901 2933 5590 5622 5664
Size: 32 135 32 1329 32 2657 32 42
Cuts at: 5664 5696 1373
Size: 32 2626
Fragments arranged by size:
2657 2626 1329 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2953 4293 4539 669
Size: 988 281 1015 1340 246 3079
Fragments arranged by size:
3079 1340 1015 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6949
HpaI GTT'AAC
Cuts at: 4539 4539
Size: 6949
KpnI G_GTAC'C
Cuts at: 4274 4274
Size: 6949
MfeI C'AATT_G
Cuts at: 2878 3032 3536 4548 2878
Size: 154 504 1012 5279
Fragments arranged by size:
5279 1012 504 154
MluI A'CGCG_T
Cuts at: 6825 6825
Size: 6949
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2119 2328 3442 6063 6247 1305
Size: 814 209 1114 2621 184 2007
Fragments arranged by size:
2621 2007 1114 814 209 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5127
Fragments arranged by size:
5127 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3239 4907 5266 5425 518
Size: 648 2073 1668 359 159 2042
Fragments arranged by size:
2073 2042 1668 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4365 5042 6190 6435 1606
Size: 2759 677 1148 245 2120
Fragments arranged by size:
2759 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6397
NdeI CA'TA_TG
Cuts at: 387 3670 387
Size: 3283 3666
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6949
NotI GC'GGCC_GC
Cuts at: 4396 4396
Size: 6949
NsiI A_TGCA'T
Cuts at: 3670 3670
Size: 6949
NspI r_CATG'y
Cuts at: 1132 1229 2664 4233 4307 5852 1132
Size: 97 1435 1569 74 1545 2229
Fragments arranged by size:
2229 1569 1545 1435 97 74
PasI CC'CwG_GG
Cuts at: 1348 2859 1348
Size: 1511 5438
PciI A'CATG_T
Cuts at: 2660 4229 5848 2660
Size: 1569 1619 3761
Fragments arranged by size:
3761 1619 1569
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2642 3324 3365 1108
Size: 1534 682 41 4692
Fragments arranged by size:
4692 1534 682 41
PfoI T'CCnGG_A
Cuts at: 2905 2905
Size: 6949
PmeI GTTT'AAAC
Cuts at: 4253 4253
Size: 6949
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3105 3137 5018 5050 6531 6563 3105
Size: 32 1881 32 1481 32 3491
Fragments arranged by size:
3491 1881 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2457 1613
Size: 844 6105
PsiI TTA'TAA
Cuts at: 3531 4519 3531
Size: 988 5961
PspOMI G'GGCC_C
Cuts at: 2033 2033
Size: 6949
PstI C_TGCA'G
Cuts at: 830 2459 3427 3810 4056 4084 4301 830
Size: 1629 968 383 246 28 217 3478
Fragments arranged by size:
3478 1629 968 383 246 217 28
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6949
SacI G_AGCT'C
Cuts at: 3876 3876
Size: 6949
SalI G'TCGA_C
Cuts at: 1936 4291 1936
Size: 2355 4594
SapI GCTCTTCn'nnn_
Cuts at: 2880 3993 2880
Size: 1113 5836
SbfI CC_TGCA'GG
Cuts at: 4301 4301
Size: 6949
ScaI AGT'ACT
Cuts at: 2058 5197 6909 2058
Size: 3139 1712 2098
Fragments arranged by size:
3139 2098 1712
SexAI A'CCwGG_T
Cuts at: 2073 3117 2073
Size: 1044 5905
SfcI C'TryA_G
Cuts at: 826 1045 2151 2455 3423 3806 4052 4080 4297
Size: 219 1106 304 968 383 246 28 217
Cuts at: 4297 5432 6113 6304 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1106 968 681 383 304 246
219 217 191 28
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6949
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6949
SmaI CCC'GGG
Cuts at: 1872 4276 1872
Size: 2404 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3614 4041 5053 5954 6216 820
Size: 197 926 1671 427 1012 901 262 1553
Fragments arranged by size:
1671 1553 1012 926 901 427 262 197
SnaBI TAC'GTA
Cuts at: 493 4740 493
Size: 4247 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6949
SphI G_CATG'C
Cuts at: 1132 4307 1132
Size: 3175 3774
SspI AAT'ATT
Cuts at: 5 52 2588 4873 5
Size: 47 2536 2285 2081
Fragments arranged by size:
2536 2285 2081 47
StuI AGG'CCT
Cuts at: 1517 3297 1517
Size: 1780 5169
StyI C'CwwG_G
Cuts at: 513 1065 3101 3558 4388 513
Size: 552 2036 457 830 3074
Fragments arranged by size:
3074 2036 830 552 457
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2439 4976 4993 5146 5331 5776 325
Size: 1255 859 2537 17 153 185 445 1498
Fragments arranged by size:
2537 1498 1255 859 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2056 3941 5195
Size: 274 80 33 51 1443 79 1885 1254
Cuts at: 5195 6907 96
Size: 1712 138
Fragments arranged by size:
1885 1712 1443 1254 274 138 80 79
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2433 2465 3468 3500 4227 4259 6909 6941 2433
Size: 32 1003 32 727 32 2650 32 2441
Fragments arranged by size:
2650 2441 1003 727 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6949
XbaI T'CTAG_A
Cuts at: 4285 4285
Size: 6949
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3255 3255
Size: 6949
XhoI C'TCGA_G
Cuts at: 1943 4041 1943
Size: 2098 4851
XmaI C'CCGG_G
Cuts at: 1870 4274 1870
Size: 2404 4545
XmnI GAAnn'nnTTC
Cuts at: 3681 3828 5078 3681
Size: 147 1250 5552
Fragments arranged by size:
5552 1250 147
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4757 276
Size: 53 83 186 936 3223 2468
Fragments arranged by size:
3223 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BsmBI BstZ17I DraIII EcoO109I EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PpuMI PspXI PsrI PvuII RsrII SacII SanDI SfiI
SfoI SrfI SwaI