Restriction map for
pFN21AB7989
(Circular) (Six-base) MAPSORT of: pFN21AB7989.seq Check: 1556 from: 1 to: 6031
pFN21AB7989 6031 bp
With 182 enzymes: SgfI *
July 6, 2012 17:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3841 278
Size: 53 83 186 936 2305 2468
Fragments arranged by size:
2468 2305 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3374 1937
Size: 1437 4594
Acc65I G'GTAC_C
Cuts at: 3352 3352
Size: 6031
AclI AA'CG_TT
Cuts at: 1563 4158 4531 1563
Size: 2595 373 3063
Fragments arranged by size:
3063 2595 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4094 5478 5746 1632
Size: 2462 1384 268 1917
Fragments arranged by size:
2462 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5834
AflIII A'CryG_T
Cuts at: 1203 4930 5907 1203
Size: 3727 977 1327
Fragments arranged by size:
3727 1327 977
AgeI A'CCGG_T
Cuts at: 4862 4862
Size: 6031
AhdI GACnn_n'nnGTC
Cuts at: 4760 4760
Size: 6031
AleI CACnn'nnGTG
Cuts at: 1166 2408 1166
Size: 1242 4789
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3050 1184
Size: 1866 4165
AlwNI CAG_nnn'CTG
Cuts at: 3774 5346 3774
Size: 1572 4459
ApaI G_GGCC'C
Cuts at: 2071 2071
Size: 6031
ApaLI G'TGCA_C
Cuts at: 2012 2918 4088 5244 2012
Size: 906 1170 1156 2799
Fragments arranged by size:
2799 1170 1156 906
ApoI r'AATT_y
Cuts at: 1493 2150 3340 3566 3718 5827 1493
Size: 657 1190 226 152 2109 1697
Fragments arranged by size:
2109 1697 1190 657 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4585 160
Size: 872 3553 1606
Fragments arranged by size:
3553 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6031
AvaI C'yCGr_G
Cuts at: 1870 1943 2747 2928 3007 3356 1870
Size: 73 804 181 79 349 4545
Fragments arranged by size:
4545 804 349 181 79 73
BamHI G'GATC_C
Cuts at: 3361 3728 3361
Size: 367 5664
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2107 2209 2266 2356 3352
Size: 325 217 627 320 102 57 90 996
Cuts at: 3352 4807 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 996 627 325 320 217 102
90 57
BanII G_rGCy'C
Cuts at: 1391 2071 2847 3135 3350 1391
Size: 680 776 288 215 4072
Fragments arranged by size:
4072 776 680 288 215
BbeI G_GCGC'C
Cuts at: 2111 2213 2270 2111
Size: 102 57 5872
Fragments arranged by size:
5872 102 57
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2647 928
Size: 971 748 4312
Fragments arranged by size:
4312 971 748
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4222 4256 1903
Size: 34 50 34 2201 34 3678
Fragments arranged by size:
3678 2201 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3923 5133 3923
Size: 1210 4821
BclI T'GATC_A
Cuts at: 1548 2556 1548
Size: 1008 5023
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2066 2303 2628
Size: 107 122 71 631 605 394 237 325
Cuts at: 2628 2674 4642 136
Size: 46 1968 1525
Fragments arranged by size:
1968 1525 631 605 394 325 237 122
107 71 46
BglII A'GATC_T
Cuts at: 3054 6026 3054
Size: 2972 3059
BlpI GC'TnA_GC
Cuts at: 2687 3448 2687
Size: 761 5270
Bme1580I G_kGCm'C
Cuts at: 1790 2016 2071 2415 2571 2670 2922 4092 5248
Size: 226 55 344 156 99 252 1170 1156
Cuts at: 5248 1790
Size: 2573
Fragments arranged by size:
2573 1170 1156 344 252 226 156 99
55
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6031
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3762 4720 442
Size: 416 53 83 399 24 2345 958 1753
Fragments arranged by size:
2345 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6031
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2522 3149 3254 4691 1239
Size: 1283 627 105 1437 2579
Fragments arranged by size:
2579 1437 1283 627 105
Bpu10I CC'TnA_GC
Cuts at: 3084 3084
Size: 6031
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3227 4156 5021 5319 3227
Size: 929 865 298 3939
Fragments arranged by size:
3939 929 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4694 882
Size: 617 3195 2219
Fragments arranged by size:
3195 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3822 493
Size: 711 2618 2702
Fragments arranged by size:
2702 2618 711
BsaBI GATnn'nnATC
Cuts at: 6025 6025
Size: 6031
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2108 2210 2217 2267
Size: 53 83 186 936 575 102 7 50
Cuts at: 2267 3838 4220 4855 275
Size: 1571 382 635 1451
Fragments arranged by size:
1571 1451 936 635 575 382 186 102
83 53 50 7
BsaWI w'CCGG_w
Cuts at: 4464 4862 5136 5283 5694 4464
Size: 398 274 147 411 4801
Fragments arranged by size:
4801 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2006 2036 2901 2931 1178
Size: 30 165 30 603 30 865 30 4278
Fragments arranged by size:
4278 865 603 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2280 2351 1186
Size: 1094 71 4866
Fragments arranged by size:
4866 1094 71
BseYI C'CCAG_C
Cuts at: 2047 2962 3100 3115 5234 5730 2047
Size: 915 138 15 2119 496 2348
Fragments arranged by size:
2348 2119 915 496 138 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2408 2747 3002 1163
Size: 573 672 339 255 4192
Fragments arranged by size:
4192 672 573 339 255
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2133 2674 3481 4242 5270 1540
Size: 275 186 132 541 807 761 1028 2301
Fragments arranged by size:
2301 1028 807 761 541 275 186 132
BsiHKAI G_wGCw'C
Cuts at: 2016 2922 4092 4177 5248 2016
Size: 906 1170 85 1071 2799
Fragments arranged by size:
2799 1170 1071 906 85
BsmI GAATG_Cn'
Cuts at: 3542 3635 3542
Size: 93 5938
BsmBI CGTCTCn'nnnn_
Cuts at: 2085 2945 2085
Size: 860 5171
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2016 2071 2415 2571 2670 2847 2922
Size: 399 226 55 344 156 99 177 75
Cuts at: 2922 3135 3350 4092 4177 5248 1391
Size: 213 215 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 344 226 215 213
177 156 99 85 75 55
BspEI T'CCGG_A
Cuts at: 5694 5694
Size: 6031
BspHI T'CATG_A
Cuts at: 3920 5650 3920
Size: 1730 4301
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2517 2859 3386 844
Size: 1673 342 527 3489
Fragments arranged by size:
3489 1673 527 342
BsrBI CCG'CTC
Cuts at: 1394 1823 2116 3093 3918 1394
Size: 429 293 977 825 3507
Fragments arranged by size:
3507 977 825 429 293
BsrDI GCAATG_nn'
Cuts at: 65 1248 2523 4526 4700 65
Size: 1183 1275 2003 174 1396
Fragments arranged by size:
2003 1396 1275 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2060 2104 2128 2673 2811 4675 4862
Size: 110 250 44 24 545 138 1864 187
Cuts at: 4862 1700
Size: 2869
Fragments arranged by size:
2869 1864 545 250 187 138 110 44
24
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6031
BssHII G'CGCG_C
Cuts at: 1925 2442 1925
Size: 517 5514
BssSI C'ACGA_G
Cuts at: 2333 4091 5103 2333
Size: 1758 1012 3261
Fragments arranged by size:
3261 1758 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6031
BstBI TT'CG_AA
Cuts at: 4835 4835
Size: 6031
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6031
BstYI r'GATC_y
Cuts at: 1972 3054 3361 3728 4113 4130 5582 5668 5680
Size: 1082 307 367 385 17 1452 86 12
Cuts at: 5680 6026 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1082 385 367 346 307 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2292 2292
Size: 6031
BtgI C'CryG_G
Cuts at: 513 1065 2028 3175 513
Size: 552 963 1147 3369
Fragments arranged by size:
3369 1147 963 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5292
Fragments arranged by size:
5292 683 56
BtsI GCAGTG_nn'
Cuts at: 2403 2681 3210 3277 3286 3547 4341 4361 2403
Size: 278 529 67 9 261 794 20 4073
Fragments arranged by size:
4073 794 529 278 261 67 20 9
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6031
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5996
DraI TTT'AAA
Cuts at: 3335 3335
Size: 6031
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5038 809
Size: 507 3722 1802
Fragments arranged by size:
3722 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2130 2433 2610
Size: 54 1425 50 275 18 300 303 177
Cuts at: 2610 2671 2860 3478 4367 5996 8
Size: 61 189 618 889 1629 43
Fragments arranged by size:
1629 1425 889 618 303 300 275 189
177 61 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2130 2671 3478 1537
Size: 275 318 541 807 4090
Fragments arranged by size:
4090 807 541 318 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3961 1353
Size: 2608 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2014 4618 4992 5138 387
Size: 1064 138 425 2604 374 146 1280
Fragments arranged by size:
2604 1280 1064 425 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2522 3149 3254 4094 4691 5478 5746
Size: 393 890 627 105 840 597 787 268
Cuts at: 5746 1239
Size: 1524
Fragments arranged by size:
1524 890 840 787 627 597 393 268
105
EcoO109I rG'GnC_Cy
Cuts at: 2067 2248 2506 2067
Size: 181 258 5592
Fragments arranged by size:
5592 258 181
EcoRI G'AATT_C
Cuts at: 2150 3340 3718 2150
Size: 1190 378 4463
Fragments arranged by size:
4463 1190 378
FspI TGC'GCA
Cuts at: 4537 4537
Size: 6031
HaeII r_GCGC'y
Cuts at: 1678 2038 2111 2213 2270 5178 1678
Size: 360 73 102 57 2908 2531
Fragments arranged by size:
2908 2531 360 102 73 57
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4672 4704 4746 4778 1373
Size: 32 135 32 3100 32 42 32 2626
Fragments arranged by size:
3100 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3375 3621 669
Size: 988 281 1437 246 3079
Fragments arranged by size:
3079 1437 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6031
HpaI GTT'AAC
Cuts at: 3621 3621
Size: 6031
KasI G'GCGC_C
Cuts at: 2107 2209 2266 2107
Size: 102 57 5872
Fragments arranged by size:
5872 102 57
KpnI G_GTAC'C
Cuts at: 3356 3356
Size: 6031
MfeI C'AATT_G
Cuts at: 3630 3630
Size: 6031
MluI A'CGCG_T
Cuts at: 5907 5907
Size: 6031
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5145 5329 1305
Size: 3840 184 2007
Fragments arranged by size:
3840 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2612 10
Size: 54 1425 343 780 3429
Fragments arranged by size:
3429 1425 780 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2408 3989 4348 4507 518
Size: 648 843 399 1581 359 159 2042
Fragments arranged by size:
2042 1581 843 648 399 359 159
MspA1I CmG'CkG
Cuts at: 1606 2057 2141 2194 2731 2858 2975 3100 3447
Size: 451 84 53 537 127 117 125 347
Cuts at: 3447 4124 5272 5517 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 537 451 347 245 127
125 117 84 53
NaeI GCC'GGC
Cuts at: 2062 2106 2130 2675 2062
Size: 44 24 545 5418
Fragments arranged by size:
5418 545 44 24
NarI GG'CG_CC
Cuts at: 2108 2210 2267 2108
Size: 102 57 5872
Fragments arranged by size:
5872 102 57
NcoI C'CATG_G
Cuts at: 513 1065 2028 3175 513
Size: 552 963 1147 3369
Fragments arranged by size:
3369 1147 963 552
NdeI CA'TA_TG
Cuts at: 387 2254 387
Size: 1867 4164
NgoMIV G'CCGG_C
Cuts at: 2060 2104 2128 2673 2060
Size: 44 24 545 5418
Fragments arranged by size:
5418 545 44 24
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6031
NotI GC'GGCC_GC
Cuts at: 3478 3478
Size: 6031
NspI r_CATG'y
Cuts at: 1132 1229 3037 3389 4934 1132
Size: 97 1808 352 1545 2229
Fragments arranged by size:
2229 1808 1545 352 97
PasI CC'CwG_GG
Cuts at: 1348 2374 1348
Size: 1026 5005
PciI A'CATG_T
Cuts at: 4930 4930
Size: 6031
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6031
PfoI T'CCnGG_A
Cuts at: 3126 3126
Size: 6031
PmeI GTTT'AAAC
Cuts at: 3335 3335
Size: 6031
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2942 2974 4100 4132 5613 5645 2942
Size: 32 1126 32 1481 32 3328
Fragments arranged by size:
3328 1481 1126 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2248 2506 2248
Size: 258 5773
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6031
PsiI TTA'TAA
Cuts at: 3601 3601
Size: 6031
PspOMI G'GGCC_C
Cuts at: 2067 2067
Size: 6031
PstI C_TGCA'G
Cuts at: 830 2514 3383 830
Size: 1684 869 3478
Fragments arranged by size:
3478 1684 869
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6031
PvuII CAG'CTG
Cuts at: 2057 2141 2858 2975 2057
Size: 84 717 117 5113
Fragments arranged by size:
5113 717 117 84
SalI G'TCGA_C
Cuts at: 1936 3373 1936
Size: 1437 4594
SanDI GG'GwC_CC
Cuts at: 2248 2248
Size: 6031
SbfI CC_TGCA'GG
Cuts at: 2514 3383 2514
Size: 869 5162
ScaI AGT'ACT
Cuts at: 4279 5991 4279
Size: 1712 4319
SfcI C'TryA_G
Cuts at: 826 1045 2510 3379 4514 5195 5386 826
Size: 219 1465 869 1135 681 191 1471
Fragments arranged by size:
1471 1465 1135 869 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2303 2303
Size: 6031
SfoI GGC'GCC
Cuts at: 2109 2211 2268 2109
Size: 102 57 5872
Fragments arranged by size:
5872 102 57
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6031
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6031
SmaI CCC'GGG
Cuts at: 1872 3009 3358 1872
Size: 1137 349 4545
Fragments arranged by size:
4545 1137 349
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3242 4135 5036 5298 820
Size: 197 926 1299 893 901 262 1553
Fragments arranged by size:
1553 1299 926 901 893 262 197
SnaBI TAC'GTA
Cuts at: 493 3822 493
Size: 3329 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6031
SphI G_CATG'C
Cuts at: 1132 3037 3389 1132
Size: 1905 352 3774
Fragments arranged by size:
3774 1905 352
SspI AAT'ATT
Cuts at: 5 52 3955 5
Size: 47 3903 2081
Fragments arranged by size:
3903 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6031
StyI C'CwwG_G
Cuts at: 513 1065 2028 2607 2967 3175 3297 3470 513
Size: 552 963 579 360 208 122 173 3074
Fragments arranged by size:
3074 963 579 552 360 208 173 122
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3188 4058 4075 4228 4413 4858 325
Size: 1255 1608 870 17 153 185 445 1498
Fragments arranged by size:
1608 1498 1255 870 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2703 3139 4277
Size: 274 80 33 51 1443 726 436 1138
Cuts at: 4277 5989 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1138 726 436 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2006 2038 2901 2933 5991 6023 2006
Size: 32 863 32 3058 32 2014
Fragments arranged by size:
3058 2014 863 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6031
XbaI T'CTAG_A
Cuts at: 3367 3367
Size: 6031
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2620 3182 2620
Size: 562 5469
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6031
XmaI C'CCGG_G
Cuts at: 1870 3007 3356 1870
Size: 1137 349 4545
Fragments arranged by size:
4545 1137 349
XmnI GAAnn'nnTTC
Cuts at: 4160 4160
Size: 6031
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3839 276
Size: 53 83 186 936 2305 2468
Fragments arranged by size:
2468 2305 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SanDI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI NruI NsiI PacI PmlI PspXI
PsrI RsrII SacI SacII SapI SexAI SrfI SwaI