Restriction map for
pFN21AB7988
(Circular) (Six-base) MAPSORT of: pFN21AB7988.seq Check: 8411 from: 1 to: 5680
pFN21AB7988 5680 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3490 278
Size: 53 83 186 936 1954 2468
Fragments arranged by size:
2468 1954 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3023 1937
Size: 1086 4594
Acc65I G'GTAC_C
Cuts at: 3001 3001
Size: 5680
AclI AA'CG_TT
Cuts at: 1563 3807 4180 1563
Size: 2244 373 3063
Fragments arranged by size:
3063 2244 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3743 5127 5395 1632
Size: 2111 1384 268 1917
Fragments arranged by size:
2111 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5483
AflIII A'CryG_T
Cuts at: 1203 2505 2907 4579 5556 1203
Size: 1302 402 1672 977 1327
Fragments arranged by size:
1672 1327 1302 977 402
AgeI A'CCGG_T
Cuts at: 4511 4511
Size: 5680
AhdI GACnn_n'nnGTC
Cuts at: 4409 4409
Size: 5680
AleI CACnn'nnGTG
Cuts at: 1166 2198 1166
Size: 1032 4648
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2546 1184
Size: 1362 4318
AlwNI CAG_nnn'CTG
Cuts at: 2710 3423 4995 2710
Size: 713 1572 3395
Fragments arranged by size:
3395 1572 713
ApaI G_GGCC'C
Cuts at: 2262 2262
Size: 5680
ApaLI G'TGCA_C
Cuts at: 3737 4893 3737
Size: 1156 4524
ApoI r'AATT_y
Cuts at: 1493 2989 3215 3367 5476 1493
Size: 1496 226 152 2109 1697
Fragments arranged by size:
2109 1697 1496 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4234 160
Size: 872 3202 1606
Fragments arranged by size:
3202 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5680
AvaI C'yCGr_G
Cuts at: 1870 1943 2117 2180 2255 2442 3005 1870
Size: 73 174 63 75 187 563 4545
Fragments arranged by size:
4545 563 187 174 75 73 63
BamHI G'GATC_C
Cuts at: 3010 3377 3010
Size: 367 5313
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2140 2833 2918 3001 4456
Size: 325 217 627 353 693 85 83 1455
Cuts at: 4456 618
Size: 1842
Fragments arranged by size:
1842 1455 693 627 353 325 217 85
83
BanII G_rGCy'C
Cuts at: 1391 2111 2207 2262 2999 1391
Size: 720 96 55 737 4072
Fragments arranged by size:
4072 737 720 96 55
BbeI G_GCGC'C
Cuts at: 2144 2144
Size: 5680
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2501 928
Size: 971 602 4107
Fragments arranged by size:
4107 971 602
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2908 2942 3871 3905 1903
Size: 34 50 34 887 34 929 34 3678
Fragments arranged by size:
3678 929 887 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3572 4782 3572
Size: 1210 4470
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5680
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2222 2289 4291
Size: 107 122 71 631 605 550 67 2002
Cuts at: 4291 136
Size: 1525
Fragments arranged by size:
2002 1525 631 605 550 122 107 71
67
BglII A'GATC_T
Cuts at: 5675 5675
Size: 5680
BlpI GC'TnA_GC
Cuts at: 3097 3097
Size: 5680
Bme1580I G_kGCm'C
Cuts at: 1790 2262 2838 2921 3741 4897 1790
Size: 472 576 83 820 1156 2573
Fragments arranged by size:
2573 1156 820 576 472 83
BmgBI CAC'GTC
Cuts at: 1318 2908 1318
Size: 1590 4090
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2150 2710 3411
Size: 416 53 83 399 24 733 560 701
Cuts at: 3411 4369 442
Size: 958 1753
Fragments arranged by size:
1753 958 733 701 560 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5680
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4340 1239
Size: 3101 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3805 4670 4968 3805
Size: 865 298 4517
Fragments arranged by size:
4517 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4343 882
Size: 617 2844 2219
Fragments arranged by size:
2844 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3471 493
Size: 711 2267 2702
Fragments arranged by size:
2702 2267 711
BsaBI GATnn'nnATC
Cuts at: 5674 5674
Size: 5680
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2031 2141 3487 3869
Size: 53 83 186 936 498 110 1346 382
Cuts at: 3869 4504 275
Size: 635 1451
Fragments arranged by size:
1451 1346 936 635 498 382 186 110
83 53
BsaWI w'CCGG_w
Cuts at: 2325 2867 4113 4511 4785 4932 5343 2325
Size: 542 1246 398 274 147 411 2662
Fragments arranged by size:
2662 1246 542 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5455
Fragments arranged by size:
5455 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2380 2423 1186
Size: 1194 43 4443
Fragments arranged by size:
4443 1194 43
BseYI C'CCAG_C
Cuts at: 2095 2279 2628 2680 2855 2877 4883 5379 2095
Size: 184 349 52 175 22 2006 496 2396
Fragments arranged by size:
2396 2006 496 349 184 175 52 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2468 2740 1163
Size: 573 732 272 4103
Fragments arranged by size:
4103 732 573 272
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2640 2733 3130 3891 4919 1540
Size: 275 186 639 93 397 761 1028 2301
Fragments arranged by size:
2301 1028 761 639 397 275 186 93
BsiHKAI G_wGCw'C
Cuts at: 2606 2958 3741 3826 4897 2606
Size: 352 783 85 1071 3389
Fragments arranged by size:
3389 1071 783 352 85
BsmI GAATG_Cn'
Cuts at: 3191 3284 3191
Size: 93 5587
BsmBI CGTCTCn'nnnn_
Cuts at: 2869 2869
Size: 5680
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2111 2207 2262 2606 2838 2921 2958
Size: 399 321 96 55 344 232 83 37
Cuts at: 2958 2999 3741 3826 4897 1391
Size: 41 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 344 321 232 96
85 83 55 41 37
BspEI T'CCGG_A
Cuts at: 5343 5343
Size: 5680
BspHI T'CATG_A
Cuts at: 3569 5299 3569
Size: 1730 3950
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2440 3035 844
Size: 1596 595 3489
Fragments arranged by size:
3489 1596 595
BsrBI CCG'CTC
Cuts at: 1394 1823 3567 1394
Size: 429 1744 3507
Fragments arranged by size:
3507 1744 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4175 4349 65
Size: 1183 2927 174 1396
Fragments arranged by size:
2927 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2288 2674 2732 4324 4511 1700
Size: 110 478 386 58 1592 187 2869
Fragments arranged by size:
2869 1592 478 386 187 110 58
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5680
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5680
BssSI C'ACGA_G
Cuts at: 3740 4752 3740
Size: 1012 4668
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5680
BstBI TT'CG_AA
Cuts at: 4484 4484
Size: 5680
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5680
BstXI CCAn_nnnn'nTGG
Cuts at: 2752 2770 2752
Size: 18 5662
BstYI r'GATC_y
Cuts at: 1972 3010 3377 3762 3779 5231 5317 5329 5675
Size: 1038 367 385 17 1452 86 12 346
Cuts at: 5675 1972
Size: 1977
Fragments arranged by size:
1977 1452 1038 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2586 2697 2751 513
Size: 552 1521 111 54 3442
Fragments arranged by size:
3442 1521 552 111 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2911 487
Size: 683 56 1685 3256
Fragments arranged by size:
3256 1685 683 56
BtsI GCAGTG_nn'
Cuts at: 2417 2525 3196 3990 4010 2417
Size: 108 671 794 20 4087
Fragments arranged by size:
4087 794 671 108 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5680
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5645
DraI TTT'AAA
Cuts at: 2984 2984
Size: 5680
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4687 809
Size: 507 3371 1802
Fragments arranged by size:
3371 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2290 2637 2730
Size: 54 1425 50 275 18 460 347 93
Cuts at: 2730 3127 4016 5645 8
Size: 397 889 1629 43
Fragments arranged by size:
1629 1425 889 460 397 347 275 93
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2637 2730 3127 1537
Size: 275 825 93 397 4090
Fragments arranged by size:
4090 825 397 275 93
EarI CTCTTCn'nnn_
Cuts at: 1353 3610 1353
Size: 2257 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2727 2927 4267 4641 4787 387
Size: 1064 138 1138 200 1340 374 146 1280
Fragments arranged by size:
1340 1280 1138 1064 374 200 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3743 4340 5127 5395 1239
Size: 393 2111 597 787 268 1524
Fragments arranged by size:
2111 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2134 2259 2134
Size: 125 5555
EcoRI G'AATT_C
Cuts at: 2989 3367 2989
Size: 378 5302
FspI TGC'GCA
Cuts at: 4186 4186
Size: 5680
HaeII r_GCGC'y
Cuts at: 1678 2144 2235 2323 2681 2951 4827 1678
Size: 466 91 88 358 270 1876 2531
Fragments arranged by size:
2531 1876 466 358 270 91 88
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2436 2468 4321 4353 4395
Size: 32 135 32 864 32 1853 32 42
Cuts at: 4395 4427 1373
Size: 32 2626
Fragments arranged by size:
2626 1853 864 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3024 3270 669
Size: 988 281 1086 246 3079
Fragments arranged by size:
3079 1086 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5680
HpaI GTT'AAC
Cuts at: 3270 3270
Size: 5680
KasI G'GCGC_C
Cuts at: 2140 2140
Size: 5680
KpnI G_GTAC'C
Cuts at: 3005 3005
Size: 5680
MfeI C'AATT_G
Cuts at: 3279 3279
Size: 5680
MluI A'CGCG_T
Cuts at: 5556 5556
Size: 5680
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2136 2340 2787 2805 4794 4978 1305
Size: 831 204 447 18 1989 184 2007
Fragments arranged by size:
2007 1989 831 447 204 184 18
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3858
Fragments arranged by size:
3858 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2198 2399 3638 3997 4156 518
Size: 648 1032 201 1239 359 159 2042
Fragments arranged by size:
2042 1239 1032 648 359 201 159
MspA1I CmG'CkG
Cuts at: 1606 2576 2628 2662 3096 3773 4921 5166 1606
Size: 970 52 34 434 677 1148 245 2120
Fragments arranged by size:
2120 1148 970 677 434 245 52 34
NaeI GCC'GGC
Cuts at: 2290 2676 2290
Size: 386 5294
NarI GG'CG_CC
Cuts at: 2141 2141
Size: 5680
NcoI C'CATG_G
Cuts at: 513 1065 2586 2697 2751 513
Size: 552 1521 111 54 3442
Fragments arranged by size:
3442 1521 552 111 54
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5680
NgoMIV G'CCGG_C
Cuts at: 2288 2674 2288
Size: 386 5294
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5680
NotI GC'GGCC_GC
Cuts at: 2637 3127 2637
Size: 490 5190
NspI r_CATG'y
Cuts at: 1132 1229 3038 4583 1132
Size: 97 1809 1545 2229
Fragments arranged by size:
2229 1809 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2263 1348
Size: 915 4765
PciI A'CATG_T
Cuts at: 4579 4579
Size: 5680
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5680
PfoI T'CCnGG_A
Cuts at: 2025 2025
Size: 5680
PmeI GTTT'AAAC
Cuts at: 2984 2984
Size: 5680
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3749 3781 5262 5294 3749
Size: 32 1481 32 4135
Fragments arranged by size:
4135 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2134 2134
Size: 5680
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5680
PsiI TTA'TAA
Cuts at: 3250 3250
Size: 5680
PspOMI G'GGCC_C
Cuts at: 2258 2258
Size: 5680
PstI C_TGCA'G
Cuts at: 830 2799 2888 3032 830
Size: 1969 89 144 3478
Fragments arranged by size:
3478 1969 144 89
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5680
PvuII CAG'CTG
Cuts at: 2662 2662
Size: 5680
RsrII CG'GwC_CG
Cuts at: 2735 2735
Size: 5680
SalI G'TCGA_C
Cuts at: 1936 3022 1936
Size: 1086 4594
SbfI CC_TGCA'GG
Cuts at: 3032 3032
Size: 5680
ScaI AGT'ACT
Cuts at: 3928 5640 3928
Size: 1712 3968
SfcI C'TryA_G
Cuts at: 826 1045 2795 2884 3028 4163 4844 5035 826
Size: 219 1750 89 144 1135 681 191 1471
Fragments arranged by size:
1750 1471 1135 681 219 191 144 89
SfoI GGC'GCC
Cuts at: 2142 2142
Size: 5680
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5680
SgrAI Cr'CCGG_yG
Cuts at: 1700 2674 1700
Size: 974 4706
SmaI CCC'GGG
Cuts at: 1872 2119 2182 2257 3007 1872
Size: 247 63 75 750 4545
Fragments arranged by size:
4545 750 247 75 63
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3784 4685 4947 820
Size: 197 926 1841 901 262 1553
Fragments arranged by size:
1841 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3471 493
Size: 2978 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5680
SphI G_CATG'C
Cuts at: 1132 3038 1132
Size: 1906 3774
SrfI GCCC'GGGC
Cuts at: 2257 2257
Size: 5680
SspI AAT'ATT
Cuts at: 5 52 3604 5
Size: 47 3552 2081
Fragments arranged by size:
3552 2081 47
StuI AGG'CCT
Cuts at: 1517 2544 1517
Size: 1027 4653
StyI C'CwwG_G
Cuts at: 513 1065 2586 2697 2751 3119 513
Size: 552 1521 111 54 368 3074
Fragments arranged by size:
3074 1521 552 368 111 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2450 3707 3724 3877 4062 4507 325
Size: 1255 870 1257 17 153 185 445 1498
Fragments arranged by size:
1498 1257 1255 870 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3926 5638 96
Size: 274 80 33 51 1443 1949 1712 138
Fragments arranged by size:
1949 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5640 5672 5640
Size: 32 5648
Tth111I GACn'n_nGTC
Cuts at: 1316 2437 1316
Size: 1121 4559
XbaI T'CTAG_A
Cuts at: 3016 3016
Size: 5680
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5680
XmaI C'CCGG_G
Cuts at: 1870 2117 2180 2255 3005 1870
Size: 247 63 75 750 4545
Fragments arranged by size:
4545 750 247 75 63
XmnI GAAnn'nnTTC
Cuts at: 3809 3809
Size: 5680
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3488 276
Size: 53 83 186 936 1954 2468
Fragments arranged by size:
2468 1954 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
RsrII SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI NruI NsiI PacI PmlI
PspXI PsrI SacI SacII SanDI SapI SexAI SfiI
SwaI XcmI