Restriction map for
pFN21AB7986
(Circular) (Six-base) MAPSORT of: pFN21AB7986.seq Check: 3690 from: 1 to: 5680
pFN21AB7986 5680 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3490 278
Size: 53 83 186 936 1954 2468
Fragments arranged by size:
2468 1954 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2875 3023 1937
Size: 938 148 4594
Fragments arranged by size:
4594 938 148
Acc65I G'GTAC_C
Cuts at: 2769 3001 2769
Size: 232 5448
AclI AA'CG_TT
Cuts at: 1563 3807 4180 1563
Size: 2244 373 3063
Fragments arranged by size:
3063 2244 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2688 2918 3743 5127 5395 1632
Size: 1056 230 825 1384 268 1917
Fragments arranged by size:
1917 1384 1056 825 268 230
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5483
AflIII A'CryG_T
Cuts at: 1203 4579 5556 1203
Size: 3376 977 1327
Fragments arranged by size:
3376 1327 977
AgeI A'CCGG_T
Cuts at: 2977 4511 2977
Size: 1534 4146
AhdI GACnn_n'nnGTC
Cuts at: 4409 4409
Size: 5680
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5680
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5680
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2775 2807 2775
Size: 32 5648
AlwNI CAG_nnn'CTG
Cuts at: 2539 3423 4995 2539
Size: 884 1572 3224
Fragments arranged by size:
3224 1572 884
ApaLI G'TGCA_C
Cuts at: 2973 3737 4893 2973
Size: 764 1156 3760
Fragments arranged by size:
3760 1156 764
ApoI r'AATT_y
Cuts at: 1493 2989 3215 3367 5476 1493
Size: 1496 226 152 2109 1697
Fragments arranged by size:
2109 1697 1496 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2052 2085 4234 160
Size: 872 1020 33 2149 1606
Fragments arranged by size:
2149 1606 1020 872 33
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5680
AvaI C'yCGr_G
Cuts at: 1870 1943 2068 3005 1870
Size: 73 125 937 4545
Fragments arranged by size:
4545 937 125 73
BamHI G'GATC_C
Cuts at: 3010 3377 3010
Size: 367 5313
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2769 3001 4456 618
Size: 325 217 627 982 232 1455 1842
Fragments arranged by size:
1842 1455 982 627 325 232 217
BanII G_rGCy'C
Cuts at: 1391 2076 2999 1391
Size: 685 923 4072
Fragments arranged by size:
4072 923 685
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2878 928
Size: 971 979 3730
Fragments arranged by size:
3730 979 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3871 3905 1903
Size: 34 50 34 1850 34 3678
Fragments arranged by size:
3678 1850 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3572 4782 3572
Size: 1210 4470
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5680
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4291 136
Size: 107 122 71 631 605 2619 1525
Fragments arranged by size:
2619 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2893 5675 2893
Size: 2782 2898
BlpI GC'TnA_GC
Cuts at: 2314 3097 2314
Size: 783 4897
Bme1580I G_kGCm'C
Cuts at: 1790 2977 3741 4897 1790
Size: 1187 764 1156 2573
Fragments arranged by size:
2573 1187 1156 764
BmgBI CAC'GTC
Cuts at: 1318 2232 1318
Size: 914 4766
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2591 2933 3411
Size: 416 53 83 399 24 1174 342 478
Cuts at: 3411 4369 442
Size: 958 1753
Fragments arranged by size:
1753 1174 958 478 416 399 342 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5680
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2529 2808 4340 1239
Size: 1290 279 1532 2579
Fragments arranged by size:
2579 1532 1290 279
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3805 4670 4968 3805
Size: 865 298 4517
Fragments arranged by size:
4517 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2023 4343 882
Size: 617 524 2320 2219
Fragments arranged by size:
2320 2219 617 524
BsaAI yAC'GTr
Cuts at: 493 1204 3471 493
Size: 711 2267 2702
Fragments arranged by size:
2702 2267 711
BsaBI GATnn'nnATC
Cuts at: 5674 5674
Size: 5680
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3487 3869 4504 275
Size: 53 83 186 936 1954 382 635 1451
Fragments arranged by size:
1954 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2169 2331 2943 2977 4113 4511 4785 4932 5343
Size: 162 612 34 1136 398 274 147 411
Cuts at: 5343 2169
Size: 2506
Fragments arranged by size:
2506 1136 612 411 398 274 162 147
34
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5455
Fragments arranged by size:
5455 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2108 2294 2846 1186
Size: 922 186 552 4020
Fragments arranged by size:
4020 922 552 186
BseYI C'CCAG_C
Cuts at: 2139 2175 4883 5379 2139
Size: 36 2708 496 2440
Fragments arranged by size:
2708 2440 496 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5107
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3130 3891 4919 1540
Size: 275 186 1129 761 1028 2301
Fragments arranged by size:
2301 1129 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2977 3741 3826 4897 2977
Size: 764 85 1071 3760
Fragments arranged by size:
3760 1071 764 85
BsmI GAATG_Cn'
Cuts at: 3191 3284 3191
Size: 93 5587
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2076 2977 2999 3741 3826 4897 1391
Size: 399 286 901 22 742 85 1071 2174
Fragments arranged by size:
2174 1071 901 742 399 286 85 22
BspEI T'CCGG_A
Cuts at: 2169 5343 2169
Size: 3174 2506
BspHI T'CATG_A
Cuts at: 2715 3569 5299 2715
Size: 854 1730 3096
Fragments arranged by size:
3096 1730 854
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3035 844
Size: 2191 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3567 1394
Size: 429 1744 3507
Fragments arranged by size:
3507 1744 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4175 4349 65
Size: 1183 2927 174 1396
Fragments arranged by size:
2927 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2977 4324 4511 1700
Size: 110 1167 1347 187 2869
Fragments arranged by size:
2869 1347 1167 187 110
BsrGI T'GTAC_A
Cuts at: 96 2631 96
Size: 2535 3145
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5680
BssSI C'ACGA_G
Cuts at: 3740 4752 3740
Size: 1012 4668
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5680
BstBI TT'CG_AA
Cuts at: 4484 4484
Size: 5680
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5680
BstXI CCAn_nnnn'nTGG
Cuts at: 2920 2920
Size: 5680
BstYI r'GATC_y
Cuts at: 1972 2109 2221 2893 3010 3377 3762 3779 5231
Size: 137 112 672 117 367 385 17 1452
Cuts at: 5231 5317 5329 5675 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 672 385 367 346 137 117
112 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2706 2706
Size: 5680
BtgI C'CryG_G
Cuts at: 513 1065 2114 513
Size: 552 1049 4079
Fragments arranged by size:
4079 1049 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4941
Fragments arranged by size:
4941 683 56
BtsI GCAGTG_nn'
Cuts at: 3196 3990 4010 3196
Size: 794 20 4866
Fragments arranged by size:
4866 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5680
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2736 2771 529
Size: 35 2172 35 3438
Fragments arranged by size:
3438 2172 35 35
DraI TTT'AAA
Cuts at: 2984 2984
Size: 5680
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4687 809
Size: 507 3371 1802
Fragments arranged by size:
3371 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3127 4016 5645
Size: 54 1425 50 275 18 1297 889 1629
Cuts at: 5645 8
Size: 43
Fragments arranged by size:
1629 1425 1297 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3127 1537
Size: 275 1315 4090
Fragments arranged by size:
4090 1315 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3610 1353
Size: 2257 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4267 4641 4787 387
Size: 1064 138 2678 374 146 1280
Fragments arranged by size:
2678 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2529 2688 2808 2918 3743 4340 5127
Size: 393 897 159 120 110 825 597 787
Cuts at: 5127 5395 1239
Size: 268 1524
Fragments arranged by size:
1524 897 825 787 597 393 268 159
120 110
EcoO109I rG'GnC_Cy
Cuts at: 2536 2536
Size: 5680
EcoRI G'AATT_C
Cuts at: 2989 3367 2989
Size: 378 5302
EcoRV GAT'ATC
Cuts at: 2275 2275
Size: 5680
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2260 2260
Size: 5680
FspI TGC'GCA
Cuts at: 2024 4186 2024
Size: 2162 3518
FspAI rTGC'GCAy
Cuts at: 2024 2024
Size: 5680
HaeII r_GCGC'y
Cuts at: 1678 4827 1678
Size: 3149 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2432 2464 4321 4353 4395
Size: 32 135 32 860 32 1857 32 42
Cuts at: 4395 4427 1373
Size: 32 2626
Fragments arranged by size:
2626 1857 860 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3024 3270 669
Size: 988 281 1086 246 3079
Fragments arranged by size:
3079 1086 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5680
HpaI GTT'AAC
Cuts at: 3270 3270
Size: 5680
KpnI G_GTAC'C
Cuts at: 2773 3005 2773
Size: 232 5448
MfeI C'AATT_G
Cuts at: 3279 3279
Size: 5680
MluI A'CGCG_T
Cuts at: 5556 5556
Size: 5680
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2082 2715 4794 4978 1305
Size: 777 633 2079 184 2007
Fragments arranged by size:
2079 2007 777 633 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3858
Fragments arranged by size:
3858 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2018 2597 3638 3997 4156 518
Size: 648 852 579 1041 359 159 2042
Fragments arranged by size:
2042 1041 852 648 579 359 159
MspA1I CmG'CkG
Cuts at: 1606 2318 3096 3773 4921 5166 1606
Size: 712 778 677 1148 245 2120
Fragments arranged by size:
2120 1148 778 712 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2114 513
Size: 552 1049 4079
Fragments arranged by size:
4079 1049 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5680
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5680
NotI GC'GGCC_GC
Cuts at: 3127 3127
Size: 5680
NspI r_CATG'y
Cuts at: 1132 1229 2126 2515 3038 4583 1132
Size: 97 897 389 523 1545 2229
Fragments arranged by size:
2229 1545 897 523 389 97
PasI CC'CwG_GG
Cuts at: 1348 2852 1348
Size: 1504 4176
PciI A'CATG_T
Cuts at: 4579 4579
Size: 5680
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2232 1108
Size: 1124 4556
PmeI GTTT'AAAC
Cuts at: 2984 2984
Size: 5680
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3749 3781 5262 5294 3749
Size: 32 1481 32 4135
Fragments arranged by size:
4135 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2939 1613
Size: 1326 4354
PsiI TTA'TAA
Cuts at: 3250 3250
Size: 5680
PstI C_TGCA'G
Cuts at: 830 3032 830
Size: 2202 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5680
PvuII CAG'CTG
Cuts at: 2318 2318
Size: 5680
SalI G'TCGA_C
Cuts at: 1936 3022 1936
Size: 1086 4594
SbfI CC_TGCA'GG
Cuts at: 3032 3032
Size: 5680
ScaI AGT'ACT
Cuts at: 3928 5640 3928
Size: 1712 3968
SfcI C'TryA_G
Cuts at: 826 1045 2876 3028 4163 4844 5035 826
Size: 219 1831 152 1135 681 191 1471
Fragments arranged by size:
1831 1471 1135 681 219 191 152
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5680
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5680
SmaI CCC'GGG
Cuts at: 1872 2070 3007 1872
Size: 198 937 4545
Fragments arranged by size:
4545 937 198
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3784 4685 4947 820
Size: 197 926 1841 901 262 1553
Fragments arranged by size:
1841 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3471 493
Size: 2978 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5680
SphI G_CATG'C
Cuts at: 1132 2126 3038 1132
Size: 994 912 3774
Fragments arranged by size:
3774 994 912
SspI AAT'ATT
Cuts at: 5 52 3604 5
Size: 47 3552 2081
Fragments arranged by size:
3552 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5680
StyI C'CwwG_G
Cuts at: 513 1065 2114 3119 513
Size: 552 1049 1005 3074
Fragments arranged by size:
3074 1049 1005 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2665 3707 3724 3877 4062 4507 325
Size: 1255 1085 1042 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1085 1042 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2631 3926 5638
Size: 274 80 33 51 1443 654 1295 1712
Cuts at: 5638 96
Size: 138
Fragments arranged by size:
1712 1443 1295 654 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2407 2439 5640 5672 2407
Size: 32 3201 32 2415
Fragments arranged by size:
3201 2415 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5680
XbaI T'CTAG_A
Cuts at: 3016 3016
Size: 5680
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5680
XmaI C'CCGG_G
Cuts at: 1870 2068 3005 1870
Size: 198 937 4545
Fragments arranged by size:
4545 937 198
XmnI GAAnn'nnTTC
Cuts at: 3809 3809
Size: 5680
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3488 276
Size: 53 83 186 936 1954 2468
Fragments arranged by size:
2468 1954 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI
EcoNI FseI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI