Restriction map for
pFN21AB7983
(Circular) (Six-base) MAPSORT of: pFN21AB7983.seq Check: 8951 from: 1 to: 5971
pFN21AB7983 5971 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2134 2134
Size: 5971
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3781 278
Size: 53 83 186 936 2245 2468
Fragments arranged by size:
2468 2245 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2889 3314 1937
Size: 952 425 4594
Fragments arranged by size:
4594 952 425
Acc65I G'GTAC_C
Cuts at: 3292 3292
Size: 5971
AclI AA'CG_TT
Cuts at: 1563 4098 4471 1563
Size: 2535 373 3063
Fragments arranged by size:
3063 2535 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2600 2642 2775 4034 5418 5686 1632
Size: 968 42 133 1259 1384 268 1917
Fragments arranged by size:
1917 1384 1259 968 268 133 42
AfeI AGC'GCT
Cuts at: 2986 2986
Size: 5971
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5774
AflIII A'CryG_T
Cuts at: 1203 4870 5847 1203
Size: 3667 977 1327
Fragments arranged by size:
3667 1327 977
AgeI A'CCGG_T
Cuts at: 2917 4802 2917
Size: 1885 4086
AhdI GACnn_n'nnGTC
Cuts at: 4700 4700
Size: 5971
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5971
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5971
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2689 2721 2689
Size: 32 5939
AlwNI CAG_nnn'CTG
Cuts at: 3714 5286 3714
Size: 1572 4399
ApaI G_GGCC'C
Cuts at: 2116 2116
Size: 5971
ApaLI G'TGCA_C
Cuts at: 4028 5184 4028
Size: 1156 4815
ApoI r'AATT_y
Cuts at: 1493 2136 2169 3280 3506 3658 5767 1493
Size: 643 33 1111 226 152 2109 1697
Fragments arranged by size:
2109 1697 1111 643 226 152 33
AseI AT'TA_AT
Cuts at: 160 1032 4525 160
Size: 872 3493 1606
Fragments arranged by size:
3493 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5971
AvaI C'yCGr_G
Cuts at: 1870 1943 2721 3169 3296 1870
Size: 73 778 448 127 4545
Fragments arranged by size:
4545 778 448 127 73
BamHI G'GATC_C
Cuts at: 3301 3668 3301
Size: 367 5604
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2474 2506 3164 3173 3292
Size: 325 217 627 687 32 658 9 119
Cuts at: 3292 4747 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 687 658 627 325 217 119
32 9
BanII G_rGCy'C
Cuts at: 1391 2116 3290 1391
Size: 725 1174 4072
Fragments arranged by size:
4072 1174 725
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2836 928
Size: 971 937 4063
Fragments arranged by size:
4063 971 937
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4162 4196 1903
Size: 34 50 34 2141 34 3678
Fragments arranged by size:
3678 2141 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2502 3863 5073 2502
Size: 1361 1210 3400
Fragments arranged by size:
3400 1361 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5971
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4582 136
Size: 107 122 71 631 605 2910 1525
Fragments arranged by size:
2910 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2746 5966 2746
Size: 3220 2751
BlpI GC'TnA_GC
Cuts at: 3388 3388
Size: 5971
Bme1580I G_kGCm'C
Cuts at: 1790 2116 2288 2477 3167 3176 4032 5188 1790
Size: 326 172 189 690 9 856 1156 2573
Fragments arranged by size:
2573 1156 856 690 326 189 172 9
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5971
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2263 3035 3702
Size: 416 53 83 399 24 846 772 667
Cuts at: 3702 4660 442
Size: 958 1753
Fragments arranged by size:
1753 958 846 772 667 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2102 2400 1056
Size: 1046 298 4627
Fragments arranged by size:
4627 1046 298
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2212 2212
Size: 5971
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2229 2699 4631 1239
Size: 990 470 1932 2579
Fragments arranged by size:
2579 1932 990 470
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4096 4961 5259 4096
Size: 865 298 4808
Fragments arranged by size:
4808 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4634 882
Size: 617 3135 2219
Fragments arranged by size:
3135 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3762 493
Size: 711 2558 2702
Fragments arranged by size:
2702 2558 711
BsaBI GATnn'nnATC
Cuts at: 5965 5965
Size: 5971
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3778 4160 4795 275
Size: 53 83 186 936 2245 382 635 1451
Fragments arranged by size:
2245 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2462 2917 4404 4802 5076 5223 5634 2462
Size: 455 1487 398 274 147 411 2799
Fragments arranged by size:
2799 1487 455 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2273 2303 2672 2702 1178
Size: 30 165 30 870 30 369 30 4447
Fragments arranged by size:
4447 870 369 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3049 1186
Size: 1863 4108
BseYI C'CCAG_C
Cuts at: 2469 3002 3047 5174 5670 2469
Size: 533 45 2127 496 2770
Fragments arranged by size:
2770 2127 533 496 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5398
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2120 3421 4182 5210 1540
Size: 275 186 119 1301 761 1028 2301
Fragments arranged by size:
2301 1301 1028 761 275 186 119
BsiHKAI G_wGCw'C
Cuts at: 4032 4117 5188 4032
Size: 85 1071 4815
Fragments arranged by size:
4815 1071 85
BsmI GAATG_Cn'
Cuts at: 3482 3575 3482
Size: 93 5878
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2116 2288 2477 3167 3176 3290 4032
Size: 399 326 172 189 690 9 114 742
Cuts at: 4032 4117 5188 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 690 399 326 189 172
114 85 9
BspEI T'CCGG_A
Cuts at: 5634 5634
Size: 5971
BspHI T'CATG_A
Cuts at: 3860 5590 3860
Size: 1730 4241
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2134 2169 3033 3326 844
Size: 1290 35 864 293 3489
Fragments arranged by size:
3489 1290 864 293 35
BsrBI CCG'CTC
Cuts at: 1394 1823 3858 1394
Size: 429 2035 3507
Fragments arranged by size:
3507 2035 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4466 4640 65
Size: 1183 3218 174 1396
Fragments arranged by size:
3218 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2917 4615 4802 1700
Size: 110 1107 1698 187 2869
Fragments arranged by size:
2869 1698 1107 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5971
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5971
BssSI C'ACGA_G
Cuts at: 4031 5043 4031
Size: 1012 4959
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5971
BstBI TT'CG_AA
Cuts at: 4775 4775
Size: 5971
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5971
BstYI r'GATC_y
Cuts at: 1972 2746 3301 3668 4053 4070 5522 5608 5620
Size: 774 555 367 385 17 1452 86 12
Cuts at: 5620 5966 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 774 555 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 3012 513
Size: 552 1947 3472
Fragments arranged by size:
3472 1947 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5232
Fragments arranged by size:
5232 683 56
BtsI GCAGTG_nn'
Cuts at: 2063 3487 4281 4301 2063
Size: 1424 794 20 3733
Fragments arranged by size:
3733 1424 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5971
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5936
DraI TTT'AAA
Cuts at: 3275 3275
Size: 5971
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4978 809
Size: 507 3662 1802
Fragments arranged by size:
3662 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2060 2117 3257
Size: 54 1425 50 275 18 230 57 1140
Cuts at: 3257 3418 4307 5936 8
Size: 161 889 1629 43
Fragments arranged by size:
1629 1425 1140 889 275 230 161 57
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2117 3418 1537
Size: 275 305 1301 4090
Fragments arranged by size:
4090 1301 305 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2797 3901 1353
Size: 1444 1104 3423
Fragments arranged by size:
3423 1444 1104
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3118 4558 4932 5078 387
Size: 1064 138 1529 1440 374 146 1280
Fragments arranged by size:
1529 1440 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2229 2600 2642 2699 2775 4034 4631
Size: 393 597 371 42 57 76 1259 597
Cuts at: 4631 5418 5686 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1259 787 597 597 393 371 268
76 57 42
EcoO109I rG'GnC_Cy
Cuts at: 2112 2674 2112
Size: 562 5409
EcoRI G'AATT_C
Cuts at: 2136 3280 3658 2136
Size: 1144 378 4449
Fragments arranged by size:
4449 1144 378
FspI TGC'GCA
Cuts at: 4477 4477
Size: 5971
HaeII r_GCGC'y
Cuts at: 1678 2988 5118 1678
Size: 1310 2130 2531
Fragments arranged by size:
2531 2130 1310
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2273 2305 4612 4644 4686
Size: 32 135 32 701 32 2307 32 42
Cuts at: 4686 4718 1373
Size: 32 2626
Fragments arranged by size:
2626 2307 701 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2053 3315 3561 669
Size: 988 281 115 1262 246 3079
Fragments arranged by size:
3079 1262 988 281 246 115
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5971
HpaI GTT'AAC
Cuts at: 3561 3561
Size: 5971
KpnI G_GTAC'C
Cuts at: 3296 3296
Size: 5971
MfeI C'AATT_G
Cuts at: 3570 3570
Size: 5971
MluI A'CGCG_T
Cuts at: 5847 5847
Size: 5971
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2175 2604 5085 5269 1305
Size: 870 429 2481 184 2007
Fragments arranged by size:
2481 2007 870 429 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3259 10
Size: 54 1425 343 1427 2722
Fragments arranged by size:
2722 1427 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3929 4288 4447 518
Size: 648 2763 359 159 2042
Fragments arranged by size:
2763 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2022 2109 2620 3387 4064 5212 5457 1606
Size: 416 87 511 767 677 1148 245 2120
Fragments arranged by size:
2120 1148 767 677 511 416 245 87
NcoI C'CATG_G
Cuts at: 513 1065 3012 513
Size: 552 1947 3472
Fragments arranged by size:
3472 1947 552
NdeI CA'TA_TG
Cuts at: 387 3132 387
Size: 2745 3226
NheI G'CTAG_C
Cuts at: 1052 2098 2396 1052
Size: 1046 298 4627
Fragments arranged by size:
4627 1046 298
NotI GC'GGCC_GC
Cuts at: 3418 3418
Size: 5971
NspI r_CATG'y
Cuts at: 1132 1229 2431 3076 3329 4874 1132
Size: 97 1202 645 253 1545 2229
Fragments arranged by size:
2229 1545 1202 645 253 97
PasI CC'CwG_GG
Cuts at: 1348 2208 1348
Size: 860 5111
PciI A'CATG_T
Cuts at: 4870 4870
Size: 5971
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5971
PfoI T'CCnGG_A
Cuts at: 3226 3226
Size: 5971
PmeI GTTT'AAAC
Cuts at: 3275 3275
Size: 5971
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4040 4072 5553 5585 4040
Size: 32 1481 32 4426
Fragments arranged by size:
4426 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5971
PsiI TTA'TAA
Cuts at: 3541 3541
Size: 5971
PspOMI G'GGCC_C
Cuts at: 2112 2112
Size: 5971
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2638 2670 2638
Size: 32 5939
PstI C_TGCA'G
Cuts at: 830 2145 3323 830
Size: 1315 1178 3478
Fragments arranged by size:
3478 1315 1178
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5971
PvuII CAG'CTG
Cuts at: 2620 2620
Size: 5971
SalI G'TCGA_C
Cuts at: 1936 3313 1936
Size: 1377 4594
SbfI CC_TGCA'GG
Cuts at: 3323 3323
Size: 5971
ScaI AGT'ACT
Cuts at: 4219 5931 4219
Size: 1712 4259
SfcI C'TryA_G
Cuts at: 826 1045 2141 2980 3319 4454 5135 5326 826
Size: 219 1096 839 339 1135 681 191 1471
Fragments arranged by size:
1471 1135 1096 839 681 339 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5971
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5971
SmaI CCC'GGG
Cuts at: 1872 3298 1872
Size: 1426 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4075 4976 5238 820
Size: 197 926 2132 901 262 1553
Fragments arranged by size:
2132 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3762 493
Size: 3269 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5971
SphI G_CATG'C
Cuts at: 1132 3076 3329 1132
Size: 1944 253 3774
Fragments arranged by size:
3774 1944 253
SspI AAT'ATT
Cuts at: 5 52 3895 5
Size: 47 3843 2081
Fragments arranged by size:
3843 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5971
StyI C'CwwG_G
Cuts at: 513 1065 3012 3410 513
Size: 552 1947 398 3074
Fragments arranged by size:
3074 1947 552 398
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3998 4015 4168 4353 4798 325
Size: 1255 2418 17 153 185 445 1498
Fragments arranged by size:
2418 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4217 5929 96
Size: 274 80 33 51 1443 2240 1712 138
Fragments arranged by size:
2240 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5931 5963 5931
Size: 32 5939
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5971
XbaI T'CTAG_A
Cuts at: 3307 3307
Size: 5971
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3010 3010
Size: 5971
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5971
XmaI C'CCGG_G
Cuts at: 1870 3296 1870
Size: 1426 4545
XmnI GAAnn'nnTTC
Cuts at: 4100 4100
Size: 5971
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3779 276
Size: 53 83 186 936 2245 2468
Fragments arranged by size:
2468 2245 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspXI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI