Restriction map for
pFN21AB7979
(Circular) (Six-base) MAPSORT of: pFN21AB7979.seq Check: 9847 from: 1 to: 6001
pFN21AB7979 6001 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3811 278
Size: 53 83 186 936 2275 2468
Fragments arranged by size:
2468 2275 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2424 3344 1937
Size: 487 920 4594
Fragments arranged by size:
4594 920 487
Acc65I G'GTAC_C
Cuts at: 2613 3322 2613
Size: 709 5292
AclI AA'CG_TT
Cuts at: 1563 4128 4501 1563
Size: 2565 373 3063
Fragments arranged by size:
3063 2565 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2048 4064 5448 5716 1632
Size: 416 2016 1384 268 1917
Fragments arranged by size:
2016 1917 1384 416 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5804
AflIII A'CryG_T
Cuts at: 1203 3099 4900 5877 1203
Size: 1896 1801 977 1327
Fragments arranged by size:
1896 1801 1327 977
AgeI A'CCGG_T
Cuts at: 4832 4832
Size: 6001
AhdI GACnn_n'nnGTC
Cuts at: 4730 4730
Size: 6001
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6001
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6001
AlwNI CAG_nnn'CTG
Cuts at: 3052 3055 3244 3744 5316 3052
Size: 3 189 500 1572 3737
Fragments arranged by size:
3737 1572 500 189 3
ApaLI G'TGCA_C
Cuts at: 4058 5214 4058
Size: 1156 4845
ApoI r'AATT_y
Cuts at: 1493 2396 3310 3536 3688 5797 1493
Size: 903 914 226 152 2109 1697
Fragments arranged by size:
2109 1697 914 903 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2630 4555 160
Size: 872 1598 1925 1606
Fragments arranged by size:
1925 1606 1598 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6001
AvaI C'yCGr_G
Cuts at: 1870 1943 3326 1870
Size: 73 1383 4545
Fragments arranged by size:
4545 1383 73
BamHI G'GATC_C
Cuts at: 3331 3698 3331
Size: 367 5634
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2613 2804 3322 4777 618
Size: 325 217 627 826 191 518 1455 1842
Fragments arranged by size:
1842 1455 826 627 518 325 217 191
BanII G_rGCy'C
Cuts at: 1391 3320 1391
Size: 1929 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2118 928
Size: 971 219 4811
Fragments arranged by size:
4811 971 219
BbvCI CC'TCA_GC
Cuts at: 3152 3152
Size: 6001
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2258 2292 2775 2809 4192 4226 1903
Size: 34 321 34 483 34 1383 34 3678
Fragments arranged by size:
3678 1383 483 321 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3893 5103 3893
Size: 1210 4791
BclI T'GATC_A
Cuts at: 1548 2857 1548
Size: 1309 4692
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2798 3156 4612
Size: 107 122 71 631 605 1126 358 1456
Cuts at: 4612 136
Size: 1525
Fragments arranged by size:
1525 1456 1126 631 605 358 122 107
71
BglII A'GATC_T
Cuts at: 5996 5996
Size: 6001
BlpI GC'TnA_GC
Cuts at: 3418 3418
Size: 6001
Bme1580I G_kGCm'C
Cuts at: 1790 2807 4062 5218 1790
Size: 1017 1255 1156 2573
Fragments arranged by size:
2573 1255 1156 1017
BmgBI CAC'GTC
Cuts at: 1318 2923 1318
Size: 1605 4396
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2386 3732 4690
Size: 416 53 83 399 24 969 1346 958
Cuts at: 4690 442
Size: 1753
Fragments arranged by size:
1753 1346 969 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6001
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2360 3221 4661 1239
Size: 1121 861 1440 2579
Fragments arranged by size:
2579 1440 1121 861
Bpu10I CC'TnA_GC
Cuts at: 3152 3152
Size: 6001
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4126 4991 5289 4126
Size: 865 298 4838
Fragments arranged by size:
4838 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4664 882
Size: 617 3165 2219
Fragments arranged by size:
3165 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2071 3792 493
Size: 711 867 1721 2702
Fragments arranged by size:
2702 1721 867 711
BsaBI GATnn'nnATC
Cuts at: 5995 5995
Size: 6001
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2736 3808 4190 4825
Size: 53 83 186 936 1203 1072 382 635
Cuts at: 4825 275
Size: 1451
Fragments arranged by size:
1451 1203 1072 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4434 4832 5106 5253 5664 4434
Size: 398 274 147 411 4771
Fragments arranged by size:
4771 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5776
Fragments arranged by size:
5776 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2441 1186
Size: 1255 4746
BseYI C'CCAG_C
Cuts at: 2324 5204 5700 2324
Size: 2880 496 2625
Fragments arranged by size:
2880 2625 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2234 1163
Size: 573 498 4930
Fragments arranged by size:
4930 573 498
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3160 3451 4212 5240 1540
Size: 275 186 1159 291 761 1028 2301
Fragments arranged by size:
2301 1159 1028 761 291 275 186
BsiHKAI G_wGCw'C
Cuts at: 4062 4147 5218 4062
Size: 85 1071 4845
Fragments arranged by size:
4845 1071 85
BsmI GAATG_Cn'
Cuts at: 2872 3512 3605 2872
Size: 640 93 5268
Fragments arranged by size:
5268 640 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2807 3320 4062 4147 5218 1391
Size: 399 1017 513 742 85 1071 2174
Fragments arranged by size:
2174 1071 1017 742 513 399 85
BspEI T'CCGG_A
Cuts at: 5664 5664
Size: 6001
BspHI T'CATG_A
Cuts at: 3890 5620 3890
Size: 1730 4271
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2395 2601 3356 844
Size: 1551 206 755 3489
Fragments arranged by size:
3489 1551 755 206
BsrBI CCG'CTC
Cuts at: 1394 1823 3888 1394
Size: 429 2065 3507
Fragments arranged by size:
3507 2065 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4496 4670 65
Size: 1183 3248 174 1396
Fragments arranged by size:
3248 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4645 4832 1700
Size: 110 2835 187 2869
Fragments arranged by size:
2869 2835 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6001
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6001
BssSI C'ACGA_G
Cuts at: 4061 5073 4061
Size: 1012 4989
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6001
BstBI TT'CG_AA
Cuts at: 4805 4805
Size: 6001
BstEII G'GTnAC_C
Cuts at: 1184 2689 1184
Size: 1505 4496
BstXI CCAn_nnnn'nTGG
Cuts at: 2830 2830
Size: 6001
BstYI r'GATC_y
Cuts at: 1972 3331 3698 4083 4100 5552 5638 5650 5996
Size: 1359 367 385 17 1452 86 12 346
Cuts at: 5996 1972
Size: 1977
Fragments arranged by size:
1977 1452 1359 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2425 2425
Size: 6001
BtgI C'CryG_G
Cuts at: 513 1065 2004 2016 2401 2536 513
Size: 552 939 12 385 135 3978
Fragments arranged by size:
3978 939 552 385 135 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5262
Fragments arranged by size:
5262 683 56
BtsI GCAGTG_nn'
Cuts at: 2097 2335 3261 3517 4311 4331 2097
Size: 238 926 256 794 20 3767
Fragments arranged by size:
3767 926 794 256 238 20
ClaI AT'CG_AT
Cuts at: 1554 2268 1554
Size: 714 5287
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5966
DraI TTT'AAA
Cuts at: 3305 3305
Size: 6001
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2492 5008 809
Size: 507 1176 2516 1802
Fragments arranged by size:
2516 1802 1176 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2049 2623 3157
Size: 54 1425 50 275 18 219 574 534
Cuts at: 3157 3448 4337 5966 8
Size: 291 889 1629 43
Fragments arranged by size:
1629 1425 889 574 534 291 275 219
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3157 3448 1537
Size: 275 1345 291 4090
Fragments arranged by size:
4090 1345 291 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2340 3134 3267 3931 1353
Size: 987 794 133 664 3423
Fragments arranged by size:
3423 987 794 664 133
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4588 4962 5108 387
Size: 1064 138 2999 374 146 1280
Fragments arranged by size:
2999 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2048 2360 3221 4064 4661 5448 5716
Size: 393 416 312 861 843 597 787 268
Cuts at: 5716 1239
Size: 1524
Fragments arranged by size:
1524 861 843 787 597 416 393 312
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2030 2030
Size: 6001
EcoO109I rG'GnC_Cy
Cuts at: 3148 3163 3208 3241 3148
Size: 15 45 33 5908
Fragments arranged by size:
5908 45 33 15
EcoRI G'AATT_C
Cuts at: 2396 3310 3688 2396
Size: 914 378 4709
Fragments arranged by size:
4709 914 378
FspI TGC'GCA
Cuts at: 4507 4507
Size: 6001
HaeII r_GCGC'y
Cuts at: 1678 2039 5148 1678
Size: 361 3109 2531
Fragments arranged by size:
3109 2531 361
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3010 3042 4642 4674 4716
Size: 32 135 32 1438 32 1600 32 42
Cuts at: 4716 4748 1373
Size: 32 2626
Fragments arranged by size:
2626 1600 1438 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2497 3345 3591 669
Size: 988 281 559 848 246 3079
Fragments arranged by size:
3079 988 848 559 281 246
HindIII A'AGCT_T
Cuts at: 748 3284 748
Size: 2536 3465
HpaI GTT'AAC
Cuts at: 3591 3591
Size: 6001
KpnI G_GTAC'C
Cuts at: 2617 3326 2617
Size: 709 5292
MfeI C'AATT_G
Cuts at: 3600 3600
Size: 6001
MluI A'CGCG_T
Cuts at: 5877 5877
Size: 6001
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5115 5299 1305
Size: 3810 184 2007
Fragments arranged by size:
3810 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2625 10
Size: 54 1425 343 793 3386
Fragments arranged by size:
3386 1425 793 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2566 2828 2877 3959 4318 4477 518
Size: 648 1400 262 49 1082 359 159 2042
Fragments arranged by size:
2042 1400 1082 648 359 262 159 49
MspA1I CmG'CkG
Cuts at: 1606 2308 2393 3052 3156 3417 4094 5242 5487
Size: 702 85 659 104 261 677 1148 245
Cuts at: 5487 1606
Size: 2120
Fragments arranged by size:
2120 1148 702 677 659 261 245 104
85
NcoI C'CATG_G
Cuts at: 513 1065 2004 2401 513
Size: 552 939 397 4113
Fragments arranged by size:
4113 939 552 397
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6001
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6001
NotI GC'GGCC_GC
Cuts at: 3448 3448
Size: 6001
NspI r_CATG'y
Cuts at: 1132 1229 2283 3359 4904 1132
Size: 97 1054 1076 1545 2229
Fragments arranged by size:
2229 1545 1076 1054 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6001
PciI A'CATG_T
Cuts at: 4900 4900
Size: 6001
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6001
PmeI GTTT'AAAC
Cuts at: 3305 3305
Size: 6001
PmlI CAC'GTG
Cuts at: 2071 2071
Size: 6001
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4070 4102 5583 5615 4070
Size: 32 1481 32 4456
Fragments arranged by size:
4456 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3163 3163
Size: 6001
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6001
PsiI TTA'TAA
Cuts at: 3571 3571
Size: 6001
PstI C_TGCA'G
Cuts at: 830 2392 2798 2977 3353 830
Size: 1562 406 179 376 3478
Fragments arranged by size:
3478 1562 406 376 179
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6001
PvuII CAG'CTG
Cuts at: 2308 2393 3052 2308
Size: 85 659 5257
Fragments arranged by size:
5257 659 85
SalI G'TCGA_C
Cuts at: 1936 3343 1936
Size: 1407 4594
SapI GCTCTTCn'nnn_
Cuts at: 3134 3134
Size: 6001
SbfI CC_TGCA'GG
Cuts at: 2798 3353 2798
Size: 555 5446
ScaI AGT'ACT
Cuts at: 4249 5961 4249
Size: 1712 4289
SfcI C'TryA_G
Cuts at: 826 1045 2388 2794 2973 3349 4484 5165 5356
Size: 219 1343 406 179 376 1135 681 191
Cuts at: 5356 826
Size: 1471
Fragments arranged by size:
1471 1343 1135 681 406 376 219 191
179
SfiI GGCCn_nnn'nGGCC
Cuts at: 3156 3156
Size: 6001
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6001
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6001
SmaI CCC'GGG
Cuts at: 1872 3328 1872
Size: 1456 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4105 5006 5268 820
Size: 197 926 2162 901 262 1553
Fragments arranged by size:
2162 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3792 493
Size: 3299 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6001
SphI G_CATG'C
Cuts at: 1132 2283 3359 1132
Size: 1151 1076 3774
Fragments arranged by size:
3774 1151 1076
SspI AAT'ATT
Cuts at: 5 52 2155 3925 5
Size: 47 2103 1770 2081
Fragments arranged by size:
2103 2081 1770 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6001
StyI C'CwwG_G
Cuts at: 513 1065 2004 2401 3440 513
Size: 552 939 397 1039 3074
Fragments arranged by size:
3074 1039 939 552 397
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4028 4045 4198 4383 4828 325
Size: 1255 2448 17 153 185 445 1498
Fragments arranged by size:
2448 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2955 4247 5959
Size: 274 80 33 51 1443 978 1292 1712
Cuts at: 5959 96
Size: 138
Fragments arranged by size:
1712 1443 1292 978 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5961 5993 5961
Size: 32 5969
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6001
XbaI T'CTAG_A
Cuts at: 3337 3337
Size: 6001
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2543 2543
Size: 6001
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6001
XmaI C'CCGG_G
Cuts at: 1870 3326 1870
Size: 1456 4545
XmnI GAAnn'nnTTC
Cuts at: 4130 4130
Size: 6001
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3809 276
Size: 53 83 186 936 2275 2468
Fragments arranged by size:
2468 2275 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SexAI SfoI SrfI SwaI