Restriction map for
pFN21AB7978
(Circular) (Six-base) MAPSORT of: pFN21AB7978.seq Check: 1393 from: 1 to: 5992
pFN21AB7978 5992 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3802 278
Size: 53 83 186 936 2266 2468
Fragments arranged by size:
2468 2266 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3335 1937
Size: 1398 4594
Acc65I G'GTAC_C
Cuts at: 3313 3313
Size: 5992
AclI AA'CG_TT
Cuts at: 1563 4119 4492 1563
Size: 2556 373 3063
Fragments arranged by size:
3063 2556 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2704 4055 5439 5707 1632
Size: 1072 1351 1384 268 1917
Fragments arranged by size:
1917 1384 1351 1072 268
AfeI AGC'GCT
Cuts at: 3148 3148
Size: 5992
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5795
AflIII A'CryG_T
Cuts at: 1203 4891 5868 1203
Size: 3688 977 1327
Fragments arranged by size:
3688 1327 977
AgeI A'CCGG_T
Cuts at: 4823 4823
Size: 5992
AhdI GACnn_n'nnGTC
Cuts at: 4721 4721
Size: 5992
AleI CACnn'nnGTG
Cuts at: 1166 2531 1166
Size: 1365 4627
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5992
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2756 2788 2756
Size: 32 5960
AlwNI CAG_nnn'CTG
Cuts at: 2605 3735 5307 2605
Size: 1130 1572 3290
Fragments arranged by size:
3290 1572 1130
ApaI G_GGCC'C
Cuts at: 2178 2992 3012 2178
Size: 814 20 5158
Fragments arranged by size:
5158 814 20
ApaLI G'TGCA_C
Cuts at: 4049 5205 4049
Size: 1156 4836
ApoI r'AATT_y
Cuts at: 1493 3301 3527 3679 5788 1493
Size: 1808 226 152 2109 1697
Fragments arranged by size:
2109 1808 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2185 2185
Size: 5992
AseI AT'TA_AT
Cuts at: 160 1032 4546 160
Size: 872 3514 1606
Fragments arranged by size:
3514 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5992
AvaI C'yCGr_G
Cuts at: 1870 1943 2195 2577 2691 2858 2899 2983 3317
Size: 73 252 382 114 167 41 84 334
Cuts at: 3317 1870
Size: 4545
Fragments arranged by size:
4545 382 334 252 167 114 84 73
41
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2349 2382 2349
Size: 33 5959
BamHI G'GATC_C
Cuts at: 3322 3689 3322
Size: 367 5625
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2592 2750 3313 4768 618
Size: 325 217 627 805 158 563 1455 1842
Fragments arranged by size:
1842 1455 805 627 563 325 217 158
BanII G_rGCy'C
Cuts at: 1391 2178 2244 2586 2992 3012 3311 1391
Size: 787 66 342 406 20 299 4072
Fragments arranged by size:
4072 787 406 342 299 66 20
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5021
BbvCI CC'TCA_GC
Cuts at: 2921 2921
Size: 5992
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4183 4217 1903
Size: 34 2246 34 3678
Fragments arranged by size:
3678 2246 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3884 5094 3884
Size: 1210 4782
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5992
BfrBI ATG'CAT
Cuts at: 2851 2851
Size: 5992
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2182 2590 2694
Size: 107 122 71 631 605 510 408 104
Cuts at: 2694 3192 4603 136
Size: 498 1411 1525
Fragments arranged by size:
1525 1411 631 605 510 498 408 122
107 104 71
BglII A'GATC_T
Cuts at: 5987 5987
Size: 5992
BlpI GC'TnA_GC
Cuts at: 2153 2403 3409 2153
Size: 250 1006 4736
Fragments arranged by size:
4736 1006 250
Bme1580I G_kGCm'C
Cuts at: 1790 2178 2906 2992 3012 4053 5209 1790
Size: 388 728 86 20 1041 1156 2573
Fragments arranged by size:
2573 1156 1041 728 388 86 20
BmgBI CAC'GTC
Cuts at: 1318 2218 2347 1318
Size: 900 129 4963
Fragments arranged by size:
4963 900 129
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3723 4681 442
Size: 416 53 83 399 24 2306 958 1753
Fragments arranged by size:
2306 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5992
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2221 2413 2717 2221
Size: 192 304 5496
Fragments arranged by size:
5496 304 192
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2627 4652 1239
Size: 1388 2025 2579
Fragments arranged by size:
2579 2025 1388
Bpu10I CC'TnA_GC
Cuts at: 2921 3248 2921
Size: 327 5665
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4117 4982 5280 4117
Size: 865 298 4829
Fragments arranged by size:
4829 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4655 882
Size: 617 3156 2219
Fragments arranged by size:
3156 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3783 493
Size: 711 2579 2702
Fragments arranged by size:
2702 2579 711
BsaBI GATnn'nnATC
Cuts at: 5986 5986
Size: 5992
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3799 4181 4816 275
Size: 53 83 186 936 2266 382 635 1451
Fragments arranged by size:
2266 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4425 4823 5097 5244 5655 4425
Size: 398 274 147 411 4762
Fragments arranged by size:
4762 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2756 2786 3059 3089 1178
Size: 30 165 30 1353 30 273 30 4081
Fragments arranged by size:
4081 1353 273 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5992
BseYI C'CCAG_C
Cuts at: 2684 3182 5195 5691 2684
Size: 498 2013 496 2985
Fragments arranged by size:
2985 2013 498 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5419
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2169 2424 3109 3442 4203 5231
Size: 275 186 168 255 685 333 761 1028
Cuts at: 5231 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 685 333 275 255 186
168
BsiHKAI G_wGCw'C
Cuts at: 2244 2681 3254 4053 4138 5209 2244
Size: 437 573 799 85 1071 3027
Fragments arranged by size:
3027 1071 799 573 437 85
BsmI GAATG_Cn'
Cuts at: 3503 3596 3503
Size: 93 5899
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2178 2244 2586 2681 2906 2992 3012
Size: 399 388 66 342 95 225 86 20
Cuts at: 3012 3254 3311 4053 4138 5209 1391
Size: 242 57 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 388 342 242 225
95 86 85 66 57 20
BspEI T'CCGG_A
Cuts at: 5655 5655
Size: 5992
BspHI T'CATG_A
Cuts at: 2427 3174 3881 5611 2427
Size: 747 707 1730 2808
Fragments arranged by size:
2808 1730 747 707
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3347 844
Size: 2503 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2796 3006 3111 3879 1394
Size: 429 973 210 105 768 3507
Fragments arranged by size:
3507 973 768 429 210 105
BsrDI GCAATG_nn'
Cuts at: 65 1248 2389 2419 4487 4661 65
Size: 1183 1141 30 2068 174 1396
Fragments arranged by size:
2068 1396 1183 1141 174 30
BsrFI r'CCGG_y
Cuts at: 1700 1810 2595 2938 3086 3164 4636 4823 1700
Size: 110 785 343 148 78 1472 187 2869
Fragments arranged by size:
2869 1472 785 343 187 148 110 78
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5992
BssHII G'CGCG_C
Cuts at: 1925 2148 2157 2185 1925
Size: 223 9 28 5732
Fragments arranged by size:
5732 223 28 9
BssSI C'ACGA_G
Cuts at: 2243 2905 4052 5064 2243
Size: 662 1147 1012 3171
Fragments arranged by size:
3171 1147 1012 662
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5992
BstBI TT'CG_AA
Cuts at: 4796 4796
Size: 5992
BstEII G'GTnAC_C
Cuts at: 1184 2560 1184
Size: 1376 4616
BstXI CCAn_nnnn'nTGG
Cuts at: 2533 3203 2533
Size: 670 5322
BstYI r'GATC_y
Cuts at: 1972 3322 3689 4074 4091 5543 5629 5641 5987
Size: 1350 367 385 17 1452 86 12 346
Cuts at: 5987 1972
Size: 1977
Fragments arranged by size:
1977 1452 1350 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2169 2565 3078 3240 513
Size: 552 939 165 396 513 162 3265
Fragments arranged by size:
3265 939 552 513 396 165 162
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5253
Fragments arranged by size:
5253 683 56
BtsI GCAGTG_nn'
Cuts at: 2482 3103 3508 4302 4322 2482
Size: 621 405 794 20 4152
Fragments arranged by size:
4152 794 621 405 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5992
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5957
DraI TTT'AAA
Cuts at: 3296 3296
Size: 5992
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4999 809
Size: 507 3683 1802
Fragments arranged by size:
3683 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2166 2421 2442
Size: 54 1425 50 275 18 336 255 21
Cuts at: 2442 2940 3084 3193 3439 4328 5957 8
Size: 498 144 109 246 889 1629 43
Fragments arranged by size:
1629 1425 889 498 336 275 255 246
144 109 54 50 43 21 18
EagI C'GGCC_G
Cuts at: 1537 1812 2166 2421 3439 1537
Size: 275 354 255 1018 4090
Fragments arranged by size:
4090 1018 354 275 255
EarI CTCTTCn'nnn_
Cuts at: 1353 2483 3922 1353
Size: 1130 1439 3423
Fragments arranged by size:
3423 1439 1130
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2240 4579 4953 5099 387
Size: 1064 138 651 2339 374 146 1280
Fragments arranged by size:
2339 1280 1064 651 374 146 138
EcoICRI GAG'CTC
Cuts at: 2242 2242
Size: 5992
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2627 2704 4055 4652 5439 5707 1239
Size: 393 995 77 1351 597 787 268 1524
Fragments arranged by size:
1524 1351 995 787 597 393 268 77
EcoO109I rG'GnC_Cy
Cuts at: 2174 2644 2988 2989 3009 2174
Size: 470 344 1 20 5157
Fragments arranged by size:
5157 470 344 20 1
EcoRI G'AATT_C
Cuts at: 3301 3679 3301
Size: 378 5614
FspI TGC'GCA
Cuts at: 2249 2411 2660 4498 2249
Size: 162 249 1838 3743
Fragments arranged by size:
3743 1838 249 162
FspAI rTGC'GCAy
Cuts at: 2249 2249
Size: 5992
HaeII r_GCGC'y
Cuts at: 1678 2299 2892 3150 5139 1678
Size: 621 593 258 1989 2531
Fragments arranged by size:
2531 1989 621 593 258
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2839 2871 4633 4665 4707
Size: 32 135 32 1267 32 1762 32 42
Cuts at: 4707 4739 1373
Size: 32 2626
Fragments arranged by size:
2626 1762 1267 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3336 3582 669
Size: 988 281 1398 246 3079
Fragments arranged by size:
3079 1398 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5992
HpaI GTT'AAC
Cuts at: 3582 3582
Size: 5992
KpnI G_GTAC'C
Cuts at: 3317 3317
Size: 5992
MfeI C'AATT_G
Cuts at: 3591 3591
Size: 5992
MluI A'CGCG_T
Cuts at: 5868 5868
Size: 5992
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2799 5106 5290 1305
Size: 1494 2307 184 2007
Fragments arranged by size:
2307 2007 1494 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2444 10
Size: 54 1425 343 612 3558
Fragments arranged by size:
3558 1425 612 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2510 2531 2558 2666 3950 4309 4468
Size: 648 1344 21 27 108 1284 359 159
Cuts at: 4468 518
Size: 2042
Fragments arranged by size:
2042 1344 1284 648 359 159 108 27
21
MspA1I CmG'CkG
Cuts at: 1606 2145 2402 2873 3408 4085 5233 5478 1606
Size: 539 257 471 535 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 539 535 471 257 245
NaeI GCC'GGC
Cuts at: 2940 3088 3166 2940
Size: 148 78 5766
Fragments arranged by size:
5766 148 78
NcoI C'CATG_G
Cuts at: 513 1065 2004 3078 513
Size: 552 939 1074 3427
Fragments arranged by size:
3427 1074 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5992
NgoMIV G'CCGG_C
Cuts at: 2938 3086 3164 2938
Size: 148 78 5766
Fragments arranged by size:
5766 148 78
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5992
NotI GC'GGCC_GC
Cuts at: 3439 3439
Size: 5992
NsiI A_TGCA'T
Cuts at: 2853 2853
Size: 5992
NspI r_CATG'y
Cuts at: 1132 1229 2665 2938 3127 3350 4895 1132
Size: 97 1436 273 189 223 1545 2229
Fragments arranged by size:
2229 1545 1436 273 223 189 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5992
PciI A'CATG_T
Cuts at: 4891 4891
Size: 5992
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5992
PmeI GTTT'AAAC
Cuts at: 3296 3296
Size: 5992
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2756 2788 4061 4093 5574 5606 2756
Size: 32 1273 32 1481 32 3142
Fragments arranged by size:
3142 1481 1273 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2644 2644
Size: 5992
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5992
PsiI TTA'TAA
Cuts at: 3562 3562
Size: 5992
PspOMI G'GGCC_C
Cuts at: 2174 2988 3008 2174
Size: 814 20 5158
Fragments arranged by size:
5158 814 20
PstI C_TGCA'G
Cuts at: 830 3344 830
Size: 2514 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5992
PvuII CAG'CTG
Cuts at: 2873 2873
Size: 5992
SacI G_AGCT'C
Cuts at: 2244 2244
Size: 5992
SalI G'TCGA_C
Cuts at: 1936 3334 1936
Size: 1398 4594
SbfI CC_TGCA'GG
Cuts at: 3344 3344
Size: 5992
ScaI AGT'ACT
Cuts at: 4240 5952 4240
Size: 1712 4280
SexAI A'CCwGG_T
Cuts at: 2436 2436
Size: 5992
SfcI C'TryA_G
Cuts at: 826 1045 2884 3340 4475 5156 5347 826
Size: 219 1839 456 1135 681 191 1471
Fragments arranged by size:
1839 1471 1135 681 456 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5992
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5992
SmaI CCC'GGG
Cuts at: 1872 2579 2860 2985 3319 1872
Size: 707 281 125 334 4545
Fragments arranged by size:
4545 707 334 281 125
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4096 4997 5259 820
Size: 197 926 2153 901 262 1553
Fragments arranged by size:
2153 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3783 493
Size: 3290 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5992
SphI G_CATG'C
Cuts at: 1132 2665 2938 3127 3350 1132
Size: 1533 273 189 223 3774
Fragments arranged by size:
3774 1533 273 223 189
SspI AAT'ATT
Cuts at: 5 52 3916 5
Size: 47 3864 2081
Fragments arranged by size:
3864 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5992
StyI C'CwwG_G
Cuts at: 513 1065 2004 2746 3078 3431 513
Size: 552 939 742 332 353 3074
Fragments arranged by size:
3074 939 742 552 353 332
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2543 2698 3253 4019 4036 4189 4374
Size: 1255 963 155 555 766 17 153 185
Cuts at: 4374 4819 325
Size: 445 1498
Fragments arranged by size:
1498 1255 963 766 555 445 185 155
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4238 5950 96
Size: 274 80 33 51 1443 2261 1712 138
Fragments arranged by size:
2261 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5952 5984 5952
Size: 32 5960
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5992
XbaI T'CTAG_A
Cuts at: 3328 3328
Size: 5992
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5992
XmaI C'CCGG_G
Cuts at: 1870 2577 2858 2983 3317 1870
Size: 707 281 125 334 4545
Fragments arranged by size:
4545 707 334 281 125
XmnI GAAnn'nnTTC
Cuts at: 2492 2768 4121 2492
Size: 276 1353 4363
Fragments arranged by size:
4363 1353 276
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3800 276
Size: 53 83 186 936 2266 2468
Fragments arranged by size:
2468 2266 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AvrII BbeI BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoNI EcoRV FalI FseI KasI NarI NruI PacI
PfoI PmlI PspXI PsrI RsrII SacII SanDI SapI
SfiI SfoI SrfI SwaI XcmI