Restriction map for
pFN21AB7974
(Circular) (Six-base) MAPSORT of: pFN21AB7974.seq Check: 3578 from: 1 to: 5944
pFN21AB7974 5944 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3754 278
Size: 53 83 186 936 2218 2468
Fragments arranged by size:
2468 2218 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3287 1937
Size: 1350 4594
Acc65I G'GTAC_C
Cuts at: 3265 3265
Size: 5944
AclI AA'CG_TT
Cuts at: 1563 4071 4444 1563
Size: 2508 373 3063
Fragments arranged by size:
3063 2508 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2573 2661 4007 5391 5659 1632
Size: 941 88 1346 1384 268 1917
Fragments arranged by size:
1917 1384 1346 941 268 88
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5747
AflIII A'CryG_T
Cuts at: 1203 4843 5820 1203
Size: 3640 977 1327
Fragments arranged by size:
3640 1327 977
AgeI A'CCGG_T
Cuts at: 4775 4775
Size: 5944
AhdI GACnn_n'nnGTC
Cuts at: 4673 4673
Size: 5944
AleI CACnn'nnGTG
Cuts at: 1166 3085 1166
Size: 1919 4025
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2925 1184
Size: 1741 4203
AlwNI CAG_nnn'CTG
Cuts at: 2801 3687 5259 2801
Size: 886 1572 3486
Fragments arranged by size:
3486 1572 886
ApaI G_GGCC'C
Cuts at: 2482 2876 2482
Size: 394 5550
ApaLI G'TGCA_C
Cuts at: 4001 5157 4001
Size: 1156 4788
ApoI r'AATT_y
Cuts at: 1493 3253 3479 3631 5740 1493
Size: 1760 226 152 2109 1697
Fragments arranged by size:
2109 1760 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4498 160
Size: 872 3466 1606
Fragments arranged by size:
3466 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5944
AvaI C'yCGr_G
Cuts at: 1870 1943 2038 2274 3163 3269 1870
Size: 73 95 236 889 106 4545
Fragments arranged by size:
4545 889 236 106 95 73
BamHI G'GATC_C
Cuts at: 3274 3641 3274
Size: 367 5577
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2026 2489 2503 3265 4720
Size: 325 217 627 239 463 14 762 1455
Cuts at: 4720 618
Size: 1842
Fragments arranged by size:
1842 1455 762 627 463 325 239 217
14
BanII G_rGCy'C
Cuts at: 1391 2304 2482 2609 2660 2856 2876 3015 3098
Size: 913 178 127 51 196 20 139 83
Cuts at: 3098 3263 1391
Size: 165 4072
Fragments arranged by size:
4072 913 196 178 165 139 127 83
51 20
BbeI G_GCGC'C
Cuts at: 2030 2030
Size: 5944
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2898 928
Size: 971 999 3974
Fragments arranged by size:
3974 999 971
BbvCI CC'TCA_GC
Cuts at: 2739 2739
Size: 5944
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4135 4169 1903
Size: 34 2198 34 3678
Fragments arranged by size:
3678 2198 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3836 5046 3836
Size: 1210 4734
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5944
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2477 4555 136
Size: 107 122 71 631 605 805 2078 1525
Fragments arranged by size:
2078 1525 805 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5939 5939
Size: 5944
BlpI GC'TnA_GC
Cuts at: 3361 3361
Size: 5944
Bme1580I G_kGCm'C
Cuts at: 1790 2045 2482 2508 2876 4005 5161 1790
Size: 255 437 26 368 1129 1156 2573
Fragments arranged by size:
2573 1156 1129 437 368 255 26
BmgBI CAC'GTC
Cuts at: 1318 2398 2726 1318
Size: 1080 328 4536
Fragments arranged by size:
4536 1080 328
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2701 2879 3675
Size: 416 53 83 399 24 1284 178 796
Cuts at: 3675 4633 442
Size: 958 1753
Fragments arranged by size:
1753 1284 958 796 416 399 178 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5944
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2480 2480
Size: 5944
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2475 2519 2636 3128 4604 1239
Size: 1236 44 117 492 1476 2579
Fragments arranged by size:
2579 1476 1236 492 117 44
Bpu10I CC'TnA_GC
Cuts at: 2739 2739
Size: 5944
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2117 2288 4069 4934 5232 2117
Size: 171 1781 865 298 2829
Fragments arranged by size:
2829 1781 865 298 171
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2525 3162 4607 882
Size: 617 1026 637 1445 2219
Fragments arranged by size:
2219 1445 1026 637 617
BsaAI yAC'GTr
Cuts at: 493 1204 3735 493
Size: 711 2531 2702
Fragments arranged by size:
2702 2531 711
BsaBI GATnn'nnATC
Cuts at: 5938 5938
Size: 5944
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2027 3751 4133 4768
Size: 53 83 186 936 494 1724 382 635
Cuts at: 4768 275
Size: 1451
Fragments arranged by size:
1724 1451 936 635 494 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2379 2984 4377 4775 5049 5196 5607 2379
Size: 605 1393 398 274 147 411 2716
Fragments arranged by size:
2716 1393 605 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3104 3134 1178
Size: 30 165 30 1701 30 3988
Fragments arranged by size:
3988 1701 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2333 3125 1186
Size: 1147 792 4005
Fragments arranged by size:
4005 1147 792
BseYI C'CCAG_C
Cuts at: 2794 5147 5643 2794
Size: 2353 496 3095
Fragments arranged by size:
3095 2353 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2387 2420 2790 1163
Size: 573 651 33 370 4317
Fragments arranged by size:
4317 651 573 370 33
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3394 4155 5183 1540
Size: 275 186 1393 761 1028 2301
Fragments arranged by size:
2301 1393 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2304 2609 2856 3098 4005 4090 5161 2304
Size: 305 247 242 907 85 1071 3087
Fragments arranged by size:
3087 1071 907 305 247 242 85
BsmI GAATG_Cn'
Cuts at: 3455 3548 3455
Size: 93 5851
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2045 2304 2482 2508 2609 2660 2856
Size: 399 255 259 178 26 101 51 196
Cuts at: 2856 2876 3015 3098 3263 4005 4090 5161 1391
Size: 20 139 83 165 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 259 255 196 178
165 139 101 85 83 51 26 20
BspEI T'CCGG_A
Cuts at: 2379 2984 5607 2379
Size: 605 2623 2716
Fragments arranged by size:
2716 2623 605
BspHI T'CATG_A
Cuts at: 2428 3833 5563 2428
Size: 1405 1730 2809
Fragments arranged by size:
2809 1730 1405
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3299 844
Size: 2455 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3831 1394
Size: 429 2008 3507
Fragments arranged by size:
3507 2008 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4439 4613 65
Size: 1183 3191 174 1396
Fragments arranged by size:
3191 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2052 4588 4775 1700
Size: 110 242 2536 187 2869
Fragments arranged by size:
2869 2536 242 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5944
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5944
BssSI C'ACGA_G
Cuts at: 3081 4004 5016 3081
Size: 923 1012 4009
Fragments arranged by size:
4009 1012 923
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5944
BstBI TT'CG_AA
Cuts at: 4748 4748
Size: 5944
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5944
BstXI CCAn_nnnn'nTGG
Cuts at: 2197 2197
Size: 5944
BstYI r'GATC_y
Cuts at: 1972 3274 3641 4026 4043 5495 5581 5593 5939
Size: 1302 367 385 17 1452 86 12 346
Cuts at: 5939 1972
Size: 1977
Fragments arranged by size:
1977 1452 1302 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4453
Fragments arranged by size:
4453 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5205
Fragments arranged by size:
5205 683 56
BtsI GCAGTG_nn'
Cuts at: 2751 2773 2897 3460 4254 4274 2751
Size: 22 124 563 794 20 4421
Fragments arranged by size:
4421 794 563 124 22 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5944
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2827 2862 529
Size: 35 2263 35 3611
Fragments arranged by size:
3611 2263 35 35
DraI TTT'AAA
Cuts at: 3248 3248
Size: 5944
DraIII CAC_nnn'GTG
Cuts at: 2359 2359
Size: 5944
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4951 809
Size: 507 3635 1802
Fragments arranged by size:
3635 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2469 2894 3391
Size: 54 1425 50 275 18 639 425 497
Cuts at: 3391 4280 5909 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 639 497 425 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3391 1537
Size: 275 1579 4090
Fragments arranged by size:
4090 1579 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2549 3874 1353
Size: 1196 1325 3423
Fragments arranged by size:
3423 1325 1196
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 2188 4531 4905 5051 387
Size: 1064 138 435 164 2343 374 146 1280
Fragments arranged by size:
2343 1280 1064 435 374 164 146 138
EcoICRI GAG'CTC
Cuts at: 2302 2607 2854 3096 2302
Size: 305 247 242 5150
Fragments arranged by size:
5150 305 247 242
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2475 2519 2573 2636 2661 3128 4007
Size: 393 843 44 54 63 25 467 879
Cuts at: 4007 4604 5391 5659 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 879 843 787 597 467 393 268
63 54 44 25
EcoNI CCTnn'n_nnAGG
Cuts at: 2102 2102
Size: 5944
EcoO109I rG'GnC_Cy
Cuts at: 2258 2479 2258
Size: 221 5723
EcoRI G'AATT_C
Cuts at: 3253 3631 3253
Size: 378 5566
FspI TGC'GCA
Cuts at: 4450 4450
Size: 5944
HaeII r_GCGC'y
Cuts at: 1678 2030 5091 1678
Size: 352 3061 2531
Fragments arranged by size:
3061 2531 352
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2210 2242 3104 3136 4585
Size: 32 135 32 638 32 862 32 1449
Cuts at: 4585 4617 4659 4691 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1449 862 638 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3288 3534 669
Size: 988 281 1350 246 3079
Fragments arranged by size:
3079 1350 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5944
HpaI GTT'AAC
Cuts at: 3534 3534
Size: 5944
KasI G'GCGC_C
Cuts at: 2026 2026
Size: 5944
KpnI G_GTAC'C
Cuts at: 3269 3269
Size: 5944
MfeI C'AATT_G
Cuts at: 3543 3543
Size: 5944
MluI A'CGCG_T
Cuts at: 5820 5820
Size: 5944
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5058 5242 1305
Size: 3753 184 2007
Fragments arranged by size:
3753 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2471 10
Size: 54 1425 343 639 3483
Fragments arranged by size:
3483 1425 639 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3085 3902 4261 4420 518
Size: 648 1919 817 359 159 2042
Fragments arranged by size:
2042 1919 817 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2144 2332 2409 2801 3360 4037 5185 5430
Size: 538 188 77 392 559 677 1148 245
Cuts at: 5430 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 559 538 392 245 188
77
NaeI GCC'GGC
Cuts at: 2054 2054
Size: 5944
NarI GG'CG_CC
Cuts at: 2027 2027
Size: 5944
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4453
Fragments arranged by size:
4453 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5944
NgoMIV G'CCGG_C
Cuts at: 2052 2052
Size: 5944
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5944
NotI GC'GGCC_GC
Cuts at: 3391 3391
Size: 5944
NspI r_CATG'y
Cuts at: 1132 1229 3302 4847 1132
Size: 97 2073 1545 2229
Fragments arranged by size:
2229 2073 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2825 1348
Size: 1477 4467
PciI A'CATG_T
Cuts at: 4843 4843
Size: 5944
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2785 1108
Size: 1677 4267
PmeI GTTT'AAAC
Cuts at: 3248 3248
Size: 5944
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4013 4045 5526 5558 4013
Size: 32 1481 32 4399
Fragments arranged by size:
4399 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5944
PsiI TTA'TAA
Cuts at: 3514 3514
Size: 5944
PspOMI G'GGCC_C
Cuts at: 2478 2872 2478
Size: 394 5550
PstI C_TGCA'G
Cuts at: 830 2106 2328 2444 2904 3296 830
Size: 1276 222 116 460 392 3478
Fragments arranged by size:
3478 1276 460 392 222 116
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5944
PvuII CAG'CTG
Cuts at: 2144 2409 2801 2144
Size: 265 392 5287
Fragments arranged by size:
5287 392 265
SacI G_AGCT'C
Cuts at: 2304 2609 2856 3098 2304
Size: 305 247 242 5150
Fragments arranged by size:
5150 305 247 242
SalI G'TCGA_C
Cuts at: 1936 3286 1936
Size: 1350 4594
SbfI CC_TGCA'GG
Cuts at: 3296 3296
Size: 5944
ScaI AGT'ACT
Cuts at: 4192 5904 4192
Size: 1712 4232
SfcI C'TryA_G
Cuts at: 826 1045 2102 2324 2440 2900 3292 4427 5108
Size: 219 1057 222 116 460 392 1135 681
Cuts at: 5108 5299 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1057 681 460 392 222 219
191 116
SfiI GGCCn_nnn'nGGCC
Cuts at: 2477 2477
Size: 5944
SfoI GGC'GCC
Cuts at: 2028 2028
Size: 5944
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5944
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5944
SmaI CCC'GGG
Cuts at: 1872 2040 2276 3165 3271 1872
Size: 168 236 889 106 4545
Fragments arranged by size:
4545 889 236 168 106
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2132 2303 4048 4949 5211 820
Size: 197 926 189 171 1745 901 262 1553
Fragments arranged by size:
1745 1553 926 901 262 197 189 171
SnaBI TAC'GTA
Cuts at: 493 3735 493
Size: 3242 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5944
SphI G_CATG'C
Cuts at: 1132 3302 1132
Size: 2170 3774
SspI AAT'ATT
Cuts at: 5 52 3868 5
Size: 47 3816 2081
Fragments arranged by size:
3816 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5944
StyI C'CwwG_G
Cuts at: 513 1065 2004 2112 3383 513
Size: 552 939 108 1271 3074
Fragments arranged by size:
3074 1271 939 552 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2353 3971 3988 4141 4326 4771 325
Size: 1255 773 1618 17 153 185 445 1498
Fragments arranged by size:
1618 1498 1255 773 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4190 5902 96
Size: 274 80 33 51 1443 2213 1712 138
Fragments arranged by size:
2213 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5904 5936 5904
Size: 32 5912
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5944
XbaI T'CTAG_A
Cuts at: 3280 3280
Size: 5944
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2786 3037 2786
Size: 251 5693
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5944
XmaI C'CCGG_G
Cuts at: 1870 2038 2274 3163 3269 1870
Size: 168 236 889 106 4545
Fragments arranged by size:
4545 889 236 168 106
XmnI GAAnn'nnTTC
Cuts at: 4073 4073
Size: 5944
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3752 276
Size: 53 83 186 936 2218 2468
Fragments arranged by size:
2468 2218 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI
BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI NruI
NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII
SacII SanDI SapI SexAI SrfI SwaI