Restriction map for
pFN21AB7972
(Circular) (Six-base) MAPSORT of: pFN21AB7972.seq Check: 3283 from: 1 to: 5938
pFN21AB7972 5938 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3748 278
Size: 53 83 186 936 2212 2468
Fragments arranged by size:
2468 2212 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3083 3281 1937
Size: 1146 198 4594
Fragments arranged by size:
4594 1146 198
Acc65I G'GTAC_C
Cuts at: 3259 3259
Size: 5938
AclI AA'CG_TT
Cuts at: 1563 4065 4438 1563
Size: 2502 373 3063
Fragments arranged by size:
3063 2502 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2031 4001 5385 5653 1632
Size: 399 1970 1384 268 1917
Fragments arranged by size:
1970 1917 1384 399 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5741
AflIII A'CryG_T
Cuts at: 1203 2033 2959 4837 5814 1203
Size: 830 926 1878 977 1327
Fragments arranged by size:
1878 1327 977 926 830
AgeI A'CCGG_T
Cuts at: 4769 4769
Size: 5938
AhdI GACnn_n'nnGTC
Cuts at: 4667 4667
Size: 5938
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5938
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5938
AlwNI CAG_nnn'CTG
Cuts at: 3681 5253 3681
Size: 1572 4366
ApaLI G'TGCA_C
Cuts at: 3995 5151 3995
Size: 1156 4782
ApoI r'AATT_y
Cuts at: 1493 2512 2693 3197 3247 3473 3625 5734 1493
Size: 1019 181 504 50 226 152 2109 1697
Fragments arranged by size:
2109 1697 1019 504 226 181 152 50
AseI AT'TA_AT
Cuts at: 160 1032 4492 160
Size: 872 3460 1606
Fragments arranged by size:
3460 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5938
AvaI C'yCGr_G
Cuts at: 1870 1943 2108 3263 1870
Size: 73 165 1155 4545
Fragments arranged by size:
4545 1155 165 73
BamHI G'GATC_C
Cuts at: 2353 3268 3635 2353
Size: 915 367 4656
Fragments arranged by size:
4656 915 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2138 3259 4714 618
Size: 325 217 627 351 1121 1455 1842
Fragments arranged by size:
1842 1455 1121 627 351 325 217
BanII G_rGCy'C
Cuts at: 1391 3257 1391
Size: 1866 4072
BbeI G_GCGC'C
Cuts at: 2142 2142
Size: 5938
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4967
BbvCI CC'TCA_GC
Cuts at: 2192 2303 2192
Size: 111 5827
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4129 4163 1903
Size: 34 2192 34 3678
Fragments arranged by size:
3678 2192 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3033 3830 5040 3033
Size: 797 1210 3931
Fragments arranged by size:
3931 1210 797
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5938
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2025 2261 4549
Size: 107 122 71 631 605 353 236 2288
Cuts at: 4549 136
Size: 1525
Fragments arranged by size:
2288 1525 631 605 353 236 122 107
71
BglII A'GATC_T
Cuts at: 5933 5933
Size: 5938
BlpI GC'TnA_GC
Cuts at: 2298 3355 2298
Size: 1057 4881
Bme1580I G_kGCm'C
Cuts at: 1790 3999 5155 1790
Size: 2209 1156 2573
Fragments arranged by size:
2573 2209 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5938
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2057 2927 3200
Size: 416 53 83 399 24 640 870 273
Cuts at: 3200 3669 4627 442
Size: 469 958 1753
Fragments arranged by size:
1753 958 870 640 469 416 399 273
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5938
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2306 2663 4598 1239
Size: 1067 357 1935 2579
Fragments arranged by size:
2579 1935 1067 357
Bpu10I CC'TnA_GC
Cuts at: 2192 2303 2721 2192
Size: 111 418 5409
Fragments arranged by size:
5409 418 111
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2360 4063 4928 5226 2360
Size: 1703 865 298 3072
Fragments arranged by size:
3072 1703 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4601 882
Size: 617 3102 2219
Fragments arranged by size:
3102 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2034 3729 493
Size: 711 830 1695 2702
Fragments arranged by size:
2702 1695 830 711
BsaBI GATnn'nnATC
Cuts at: 5932 5932
Size: 5938
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2139 3745 4127 4762
Size: 53 83 186 936 606 1606 382 635
Cuts at: 4762 275
Size: 1451
Fragments arranged by size:
1606 1451 936 635 606 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2368 4371 4769 5043 5190 5601 2368
Size: 2003 398 274 147 411 2705
Fragments arranged by size:
2705 2003 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2051 2081 1178
Size: 30 165 30 648 30 5035
Fragments arranged by size:
5035 648 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5938
BseYI C'CCAG_C
Cuts at: 5141 5637 5141
Size: 496 5442
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3149 1163
Size: 573 1413 3952
Fragments arranged by size:
3952 1413 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2245 3388 4149 5177 1540
Size: 275 186 244 1143 761 1028 2301
Fragments arranged by size:
2301 1143 1028 761 275 244 186
BsiHKAI G_wGCw'C
Cuts at: 2727 3999 4084 5155 2727
Size: 1272 85 1071 3510
Fragments arranged by size:
3510 1272 1071 85
BsmI GAATG_Cn'
Cuts at: 2807 3165 3449 3542 2807
Size: 358 284 93 5203
Fragments arranged by size:
5203 358 284 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2727 3257 3999 4084 5155 1391
Size: 399 937 530 742 85 1071 2174
Fragments arranged by size:
2174 1071 937 742 530 399 85
BspEI T'CCGG_A
Cuts at: 2368 5601 2368
Size: 3233 2705
BspHI T'CATG_A
Cuts at: 2995 3827 5557 2995
Size: 832 1730 3376
Fragments arranged by size:
3376 1730 832
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3293 844
Size: 2449 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2213 3825 1394
Size: 429 390 1612 3507
Fragments arranged by size:
3507 1612 429 390
BsrDI GCAATG_nn'
Cuts at: 65 1248 4433 4607 65
Size: 1183 3185 174 1396
Fragments arranged by size:
3185 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2203 4582 4769 1700
Size: 110 393 2379 187 2869
Fragments arranged by size:
2869 2379 393 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5938
BssHII G'CGCG_C
Cuts at: 1925 2122 1925
Size: 197 5741
BssSI C'ACGA_G
Cuts at: 3998 5010 3998
Size: 1012 4926
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5938
BstBI TT'CG_AA
Cuts at: 4742 4742
Size: 5938
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5938
BstXI CCAn_nnnn'nTGG
Cuts at: 2272 2687 2272
Size: 415 5523
BstYI r'GATC_y
Cuts at: 1972 2269 2353 3268 3635 4020 4037 5489 5575
Size: 297 84 915 367 385 17 1452 86
Cuts at: 5575 5587 5933 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 915 385 367 346 297 86
84 17 12
BstZ17I GTA'TAC
Cuts at: 3084 3084
Size: 5938
BtgI C'CryG_G
Cuts at: 513 1065 2004 2256 513
Size: 552 939 252 4195
Fragments arranged by size:
4195 939 552 252
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5199
Fragments arranged by size:
5199 683 56
BtsI GCAGTG_nn'
Cuts at: 2080 3454 4248 4268 2080
Size: 1374 794 20 3750
Fragments arranged by size:
3750 1374 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5938
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5903
DraI TTT'AAA
Cuts at: 3242 3242
Size: 5938
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4945 809
Size: 507 3629 1802
Fragments arranged by size:
3629 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2262 3385 4274
Size: 54 1425 50 275 18 432 1123 889
Cuts at: 4274 5903 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1123 889 432 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3385 1537
Size: 275 1573 4090
Fragments arranged by size:
4090 1573 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3868 1353
Size: 2515 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4525 4899 5045 387
Size: 1064 138 2936 374 146 1280
Fragments arranged by size:
2936 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2031 2306 2663 4001 4598 5385 5653
Size: 393 399 275 357 1338 597 787 268
Cuts at: 5653 1239
Size: 1524
Fragments arranged by size:
1524 1338 787 597 399 393 357 275
268
EcoO109I rG'GnC_Cy
Cuts at: 2059 3127 2059
Size: 1068 4870
EcoRI G'AATT_C
Cuts at: 2693 3197 3247 3625 2693
Size: 504 50 378 5006
Fragments arranged by size:
5006 504 378 50
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2558 2558
Size: 5938
FspI TGC'GCA
Cuts at: 4444 4444
Size: 5938
HaeII r_GCGC'y
Cuts at: 1678 2142 5085 1678
Size: 464 2943 2531
Fragments arranged by size:
2943 2531 464
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2051 2083 2468 2500 3007
Size: 32 135 32 479 32 385 32 507
Cuts at: 3007 3039 4579 4611 4653 4685 1373
Size: 32 1540 32 42 32 2626
Fragments arranged by size:
2626 1540 507 479 385 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3282 3528 669
Size: 988 281 1344 246 3079
Fragments arranged by size:
3079 1344 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5938
HpaI GTT'AAC
Cuts at: 3528 3528
Size: 5938
KasI G'GCGC_C
Cuts at: 2138 2138
Size: 5938
KpnI G_GTAC'C
Cuts at: 3263 3263
Size: 5938
MfeI C'AATT_G
Cuts at: 3537 3537
Size: 5938
MluI A'CGCG_T
Cuts at: 5814 5814
Size: 5938
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2265 5052 5236 1305
Size: 960 2787 184 2007
Fragments arranged by size:
2787 2007 960 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2264 10
Size: 54 1425 343 432 3684
Fragments arranged by size:
3684 1425 432 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2997 3896 4255 4414 518
Size: 648 1831 899 359 159 2042
Fragments arranged by size:
2042 1831 899 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2234 2258 3354 4031 5179 5424 1606
Size: 628 24 1096 677 1148 245 2120
Fragments arranged by size:
2120 1148 1096 677 628 245 24
NaeI GCC'GGC
Cuts at: 2205 2205
Size: 5938
NarI GG'CG_CC
Cuts at: 2139 2139
Size: 5938
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4447
Fragments arranged by size:
4447 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5938
NgoMIV G'CCGG_C
Cuts at: 2203 2203
Size: 5938
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5938
NotI GC'GGCC_GC
Cuts at: 3385 3385
Size: 5938
NspI r_CATG'y
Cuts at: 1132 1229 2492 2963 3296 4841 1132
Size: 97 1263 471 333 1545 2229
Fragments arranged by size:
2229 1545 1263 471 333 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5938
PciI A'CATG_T
Cuts at: 2959 4837 2959
Size: 1878 4060
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5938
PfoI T'CCnGG_A
Cuts at: 3223 3223
Size: 5938
PmeI GTTT'AAAC
Cuts at: 3242 3242
Size: 5938
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4007 4039 5520 5552 4007
Size: 32 1481 32 4393
Fragments arranged by size:
4393 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2059 3127 2059
Size: 1068 4870
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5938
PsiI TTA'TAA
Cuts at: 2781 3508 2781
Size: 727 5211
PstI C_TGCA'G
Cuts at: 830 2282 2456 3290 830
Size: 1452 174 834 3478
Fragments arranged by size:
3478 1452 834 174
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5938
PvuII CAG'CTG
Cuts at: 2234 2234
Size: 5938
SacII CC_GC'GG
Cuts at: 2259 2259
Size: 5938
SalI G'TCGA_C
Cuts at: 1936 3280 1936
Size: 1344 4594
SbfI CC_TGCA'GG
Cuts at: 3290 3290
Size: 5938
ScaI AGT'ACT
Cuts at: 4186 5898 4186
Size: 1712 4226
SexAI A'CCwGG_T
Cuts at: 2382 2382
Size: 5938
SfcI C'TryA_G
Cuts at: 826 1045 2278 2452 3286 4421 5102 5293 826
Size: 219 1233 174 834 1135 681 191 1471
Fragments arranged by size:
1471 1233 1135 834 681 219 191 174
SfoI GGC'GCC
Cuts at: 2140 2140
Size: 5938
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5938
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5938
SmaI CCC'GGG
Cuts at: 1872 3265 1872
Size: 1393 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2339 4042 4943 5205 820
Size: 197 926 396 1703 901 262 1553
Fragments arranged by size:
1703 1553 926 901 396 262 197
SnaBI TAC'GTA
Cuts at: 493 3729 493
Size: 3236 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5938
SphI G_CATG'C
Cuts at: 1132 3296 1132
Size: 2164 3774
SspI AAT'ATT
Cuts at: 5 52 3862 5
Size: 47 3810 2081
Fragments arranged by size:
3810 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5938
StyI C'CwwG_G
Cuts at: 513 1065 2004 3377 513
Size: 552 939 1373 3074
Fragments arranged by size:
3074 1373 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2036 3085 3965 3982 4135 4320 4765
Size: 1255 456 1049 880 17 153 185 445
Cuts at: 4765 325
Size: 1498
Fragments arranged by size:
1498 1255 1049 880 456 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4184 5896 96
Size: 274 80 33 51 1443 2207 1712 138
Fragments arranged by size:
2207 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5898 5930 5898
Size: 32 5906
Tth111I GACn'n_nGTC
Cuts at: 1316 2063 1316
Size: 747 5191
XbaI T'CTAG_A
Cuts at: 3274 3274
Size: 5938
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5938
XmaI C'CCGG_G
Cuts at: 1870 3263 1870
Size: 1393 4545
XmnI GAAnn'nnTTC
Cuts at: 2364 4067 2364
Size: 1703 4235
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3746 276
Size: 53 83 186 936 2212 2468
Fragments arranged by size:
2468 2212 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacII SalI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BplI BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI EcoRV
FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI
PsrI RsrII SacI SanDI SapI SfiI SrfI SwaI
XcmI