Restriction map for
pFN21AB7971
(Circular) (Six-base) MAPSORT of: pFN21AB7971.seq Check: 7507 from: 1 to: 5575
pFN21AB7971 5575 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3385 278
Size: 53 83 186 936 1849 2468
Fragments arranged by size:
2468 1849 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2149 2918 1937
Size: 212 769 4594
Fragments arranged by size:
4594 769 212
Acc65I G'GTAC_C
Cuts at: 2896 2896
Size: 5575
AclI AA'CG_TT
Cuts at: 1563 2874 3702 4075 1563
Size: 1311 828 373 3063
Fragments arranged by size:
3063 1311 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3638 5022 5290 1632
Size: 2006 1384 268 1917
Fragments arranged by size:
2006 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5378
AflIII A'CryG_T
Cuts at: 1203 4474 5451 1203
Size: 3271 977 1327
Fragments arranged by size:
3271 1327 977
AgeI A'CCGG_T
Cuts at: 4406 4406
Size: 5575
AhdI GACnn_n'nnGTC
Cuts at: 4304 4304
Size: 5575
AleI CACnn'nnGTG
Cuts at: 1166 2657 1166
Size: 1491 4084
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5575
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2739 2771 2739
Size: 32 5543
AlwNI CAG_nnn'CTG
Cuts at: 2404 2453 3318 4890 2404
Size: 49 865 1572 3089
Fragments arranged by size:
3089 1572 865 49
ApaI G_GGCC'C
Cuts at: 2619 2619
Size: 5575
ApaLI G'TGCA_C
Cuts at: 3632 4788 3632
Size: 1156 4419
ApoI r'AATT_y
Cuts at: 1493 2135 2884 3110 3262 5371 1493
Size: 642 749 226 152 2109 1697
Fragments arranged by size:
2109 1697 749 642 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4129 160
Size: 872 3097 1606
Fragments arranged by size:
3097 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5575
AvaI C'yCGr_G
Cuts at: 1870 1943 2096 2900 1870
Size: 73 153 804 4545
Fragments arranged by size:
4545 804 153 73
BamHI G'GATC_C
Cuts at: 2113 2698 2905 3272 2113
Size: 585 207 367 4416
Fragments arranged by size:
4416 585 367 207
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2896 4351 618
Size: 325 217 627 1109 1455 1842
Fragments arranged by size:
1842 1455 1109 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2372 2619 2894 1391
Size: 981 247 275 4072
Fragments arranged by size:
4072 981 275 247
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2238 928
Size: 971 339 4265
Fragments arranged by size:
4265 971 339
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3766 3800 1903
Size: 34 1829 34 3678
Fragments arranged by size:
3678 1829 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2206 3467 4677 2206
Size: 1261 1210 3104
Fragments arranged by size:
3104 1261 1210
BclI T'GATC_A
Cuts at: 1548 2167 1548
Size: 619 4956
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4186 136
Size: 107 122 71 631 605 2514 1525
Fragments arranged by size:
2514 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5570 5570
Size: 5575
BlpI GC'TnA_GC
Cuts at: 2039 2992 2039
Size: 953 4622
Bme1580I G_kGCm'C
Cuts at: 1790 2619 3636 4792 1790
Size: 829 1017 1156 2573
Fragments arranged by size:
2573 1156 1017 829
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5575
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2424 3306 4264
Size: 416 53 83 399 24 1007 882 958
Cuts at: 4264 442
Size: 1753
Fragments arranged by size:
1753 1007 958 882 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5575
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2484 2619 4235 1239
Size: 1245 135 1616 2579
Fragments arranged by size:
2579 1616 1245 135
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2784 3700 4565 4863 2784
Size: 916 865 298 3496
Fragments arranged by size:
3496 916 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4238 882
Size: 617 2739 2219
Fragments arranged by size:
2739 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3366 493
Size: 711 2162 2702
Fragments arranged by size:
2702 2162 711
BsaBI GATnn'nnATC
Cuts at: 5569 5569
Size: 5575
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3382 3764 4399 275
Size: 53 83 186 936 1849 382 635 1451
Fragments arranged by size:
1849 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2076 4008 4406 4680 4827 5238 2076
Size: 1932 398 274 147 411 2413
Fragments arranged by size:
2413 1932 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2058 2088 2378 2408 1178
Size: 30 165 30 655 30 290 30 4345
Fragments arranged by size:
4345 655 290 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2342 2380 1186
Size: 1156 38 4381
Fragments arranged by size:
4381 1156 38
BseYI C'CCAG_C
Cuts at: 2729 4778 5274 2729
Size: 2049 496 3030
Fragments arranged by size:
3030 2049 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2288 1163
Size: 573 552 4450
Fragments arranged by size:
4450 573 552
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3025 3786 4814 1540
Size: 275 186 1024 761 1028 2301
Fragments arranged by size:
2301 1028 1024 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2040 2372 3636 3721 4792 2040
Size: 332 1264 85 1071 2823
Fragments arranged by size:
2823 1264 1071 332 85
BsmI GAATG_Cn'
Cuts at: 2298 3086 3179 2298
Size: 788 93 4694
Fragments arranged by size:
4694 788 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 2372 2619 2894 3636 3721 4792
Size: 399 250 332 247 275 742 85 1071
Cuts at: 4792 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 332 275 250 247
85
BspEI T'CCGG_A
Cuts at: 2076 5238 2076
Size: 3162 2413
BspHI T'CATG_A
Cuts at: 3464 5194 3464
Size: 1730 3845
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2930 844
Size: 2086 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3462 1394
Size: 429 1639 3507
Fragments arranged by size:
3507 1639 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4070 4244 65
Size: 1183 2822 174 1396
Fragments arranged by size:
2822 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4219 4406 1700
Size: 110 2409 187 2869
Fragments arranged by size:
2869 2409 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5575
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5575
BssSI C'ACGA_G
Cuts at: 3635 4647 3635
Size: 1012 4563
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5575
BstBI TT'CG_AA
Cuts at: 4379 4379
Size: 5575
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5575
BstYI r'GATC_y
Cuts at: 1972 2113 2698 2905 3272 3657 3674 5126 5212
Size: 141 585 207 367 385 17 1452 86
Cuts at: 5212 5224 5570 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 585 385 367 346 207 141
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2338 513
Size: 552 939 334 3750
Fragments arranged by size:
3750 939 552 334
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4836
Fragments arranged by size:
4836 683 56
BtsI GCAGTG_nn'
Cuts at: 2282 2664 3091 3885 3905 2282
Size: 382 427 794 20 3952
Fragments arranged by size:
3952 794 427 382 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5575
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5540
DraI TTT'AAA
Cuts at: 2140 2879 2140
Size: 739 4836
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4582 809
Size: 507 3266 1802
Fragments arranged by size:
3266 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3022 3911 5540
Size: 54 1425 50 275 18 1192 889 1629
Cuts at: 5540 8
Size: 43
Fragments arranged by size:
1629 1425 1192 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3022 1537
Size: 275 1210 4090
Fragments arranged by size:
4090 1210 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2784 3505 1353
Size: 1431 721 3423
Fragments arranged by size:
3423 1431 721
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4162 4536 4682 387
Size: 1064 138 2573 374 146 1280
Fragments arranged by size:
2573 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2370 2370
Size: 5575
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2484 2619 3638 4235 5022 5290 1239
Size: 393 852 135 1019 597 787 268 1524
Fragments arranged by size:
1524 1019 852 787 597 393 268 135
EcoO109I rG'GnC_Cy
Cuts at: 2010 2010
Size: 5575
EcoRI G'AATT_C
Cuts at: 2884 3262 2884
Size: 378 5197
EcoRV GAT'ATC
Cuts at: 2595 2595
Size: 5575
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2825 2825
Size: 5575
FspI TGC'GCA
Cuts at: 4081 4081
Size: 5575
HaeII r_GCGC'y
Cuts at: 1678 4722 1678
Size: 3044 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2058 2090 4216 4248 4290
Size: 32 135 32 486 32 2126 32 42
Cuts at: 4290 4322 1373
Size: 32 2626
Fragments arranged by size:
2626 2126 486 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2919 3165 669
Size: 988 281 981 246 3079
Fragments arranged by size:
3079 988 981 281 246
HindIII A'AGCT_T
Cuts at: 748 2578 748
Size: 1830 3745
HpaI GTT'AAC
Cuts at: 3165 3165
Size: 5575
KpnI G_GTAC'C
Cuts at: 2900 2900
Size: 5575
MfeI C'AATT_G
Cuts at: 3174 3174
Size: 5575
MluI A'CGCG_T
Cuts at: 5451 5451
Size: 5575
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2034 2044 2782 4689 4873 1305
Size: 729 10 738 1907 184 2007
Fragments arranged by size:
2007 1907 738 729 184 10
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3753
Fragments arranged by size:
3753 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2657 3533 3892 4051 518
Size: 648 1491 876 359 159 2042
Fragments arranged by size:
2042 1491 876 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2991 3668 4816 5061 1606
Size: 1385 677 1148 245 2120
Fragments arranged by size:
2120 1385 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2338 513
Size: 552 939 334 3750
Fragments arranged by size:
3750 939 552 334
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5575
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5575
NotI GC'GGCC_GC
Cuts at: 3022 3022
Size: 5575
NspI r_CATG'y
Cuts at: 1132 1229 2933 4478 1132
Size: 97 1704 1545 2229
Fragments arranged by size:
2229 1704 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2473 1348
Size: 1125 4450
PciI A'CATG_T
Cuts at: 4474 4474
Size: 5575
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2339 2853 1108
Size: 1231 514 3830
Fragments arranged by size:
3830 1231 514
PfoI T'CCnGG_A
Cuts at: 2205 2205
Size: 5575
PmeI GTTT'AAAC
Cuts at: 2879 2879
Size: 5575
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3644 3676 5157 5189 3644
Size: 32 1481 32 4030
Fragments arranged by size:
4030 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2010 2010
Size: 5575
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5575
PsiI TTA'TAA
Cuts at: 3145 3145
Size: 5575
PspOMI G'GGCC_C
Cuts at: 2615 2615
Size: 5575
PstI C_TGCA'G
Cuts at: 830 2927 830
Size: 2097 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5575
SacI G_AGCT'C
Cuts at: 2372 2372
Size: 5575
SalI G'TCGA_C
Cuts at: 1936 2917 1936
Size: 981 4594
SbfI CC_TGCA'GG
Cuts at: 2927 2927
Size: 5575
ScaI AGT'ACT
Cuts at: 3823 5535 3823
Size: 1712 3863
SfcI C'TryA_G
Cuts at: 826 1045 2923 4058 4739 4930 826
Size: 219 1878 1135 681 191 1471
Fragments arranged by size:
1878 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5575
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5575
SmaI CCC'GGG
Cuts at: 1872 2902 1872
Size: 1030 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2799 3679 4580 4842 820
Size: 197 926 856 880 901 262 1553
Fragments arranged by size:
1553 926 901 880 856 262 197
SnaBI TAC'GTA
Cuts at: 493 3366 493
Size: 2873 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5575
SphI G_CATG'C
Cuts at: 1132 2933 1132
Size: 1801 3774
SspI AAT'ATT
Cuts at: 5 52 3499 5
Size: 47 3447 2081
Fragments arranged by size:
3447 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5575
StyI C'CwwG_G
Cuts at: 513 1065 2004 2338 2487 3014 513
Size: 552 939 334 149 527 3074
Fragments arranged by size:
3074 939 552 527 334 149
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3602 3619 3772 3957 4402 325
Size: 1255 2022 17 153 185 445 1498
Fragments arranged by size:
2022 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3821 5533 96
Size: 274 80 33 51 1443 1844 1712 138
Fragments arranged by size:
1844 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5535 5567 5535
Size: 32 5543
Tth111I GACn'n_nGTC
Cuts at: 1316 2678 1316
Size: 1362 4213
XbaI T'CTAG_A
Cuts at: 2911 2911
Size: 5575
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5575
XmaI C'CCGG_G
Cuts at: 1870 2900 1870
Size: 1030 4545
XmnI GAAnn'nnTTC
Cuts at: 3704 3704
Size: 5575
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3383 276
Size: 53 83 186 936 1849 2468
Fragments arranged by size:
2468 1849 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspXI PsrI PvuII RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI