Restriction map for
pFN21AB7970
(Circular) (Six-base) MAPSORT of: pFN21AB7970.seq Check: 3430 from: 1 to: 5935
pFN21AB7970 5935 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3745 278
Size: 53 83 186 936 2209 2468
Fragments arranged by size:
2468 2209 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3278 1937
Size: 1341 4594
Acc65I G'GTAC_C
Cuts at: 2443 3256 2443
Size: 813 5122
AclI AA'CG_TT
Cuts at: 1563 2818 4062 4435 1563
Size: 1255 1244 373 3063
Fragments arranged by size:
3063 1255 1244 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2742 2912 3998 5382 5650 1632
Size: 1110 170 1086 1384 268 1917
Fragments arranged by size:
1917 1384 1110 1086 268 170
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5738
AflIII A'CryG_T
Cuts at: 1203 2431 4834 5811 1203
Size: 1228 2403 977 1327
Fragments arranged by size:
2403 1327 1228 977
AgeI A'CCGG_T
Cuts at: 4766 4766
Size: 5935
AhdI GACnn_n'nnGTC
Cuts at: 4664 4664
Size: 5935
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5935
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5935
AlwNI CAG_nnn'CTG
Cuts at: 2843 3678 5250 2843
Size: 835 1572 3528
Fragments arranged by size:
3528 1572 835
ApaI G_GGCC'C
Cuts at: 3184 3184
Size: 5935
ApaLI G'TGCA_C
Cuts at: 2140 3992 5148 2140
Size: 1852 1156 2927
Fragments arranged by size:
2927 1852 1156
ApoI r'AATT_y
Cuts at: 1493 2366 2883 3244 3470 3622 5731 1493
Size: 873 517 361 226 152 2109 1697
Fragments arranged by size:
2109 1697 873 517 361 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4489 160
Size: 872 3457 1606
Fragments arranged by size:
3457 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5935
AvaI C'yCGr_G
Cuts at: 1870 1943 3260 1870
Size: 73 1317 4545
Fragments arranged by size:
4545 1317 73
BamHI G'GATC_C
Cuts at: 2605 3265 3632 2605
Size: 660 367 4908
Fragments arranged by size:
4908 660 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2443 3256 4711 618
Size: 325 217 627 656 813 1455 1842
Fragments arranged by size:
1842 1455 813 656 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2598 2616 3184 3254 1391
Size: 1207 18 568 70 4072
Fragments arranged by size:
4072 1207 568 70 18
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2773 928
Size: 971 874 4090
Fragments arranged by size:
4090 971 874
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4126 4160 1903
Size: 34 50 34 2105 34 3678
Fragments arranged by size:
3678 2105 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3827 5037 3827
Size: 1210 4725
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5935
BfrBI ATG'CAT
Cuts at: 3048 3048
Size: 5935
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4546 136
Size: 107 122 71 631 605 2874 1525
Fragments arranged by size:
2874 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5930 5930
Size: 5935
BlpI GC'TnA_GC
Cuts at: 3352 3352
Size: 5935
Bme1580I G_kGCm'C
Cuts at: 1790 2144 3184 3996 5152 1790
Size: 354 1040 812 1156 2573
Fragments arranged by size:
2573 1156 1040 812 354
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5935
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2495 3666 4624
Size: 416 53 83 399 24 1078 1171 958
Cuts at: 4624 442
Size: 1753
Fragments arranged by size:
1753 1171 1078 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5935
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4595 1239
Size: 3356 2579
Bpu10I CC'TnA_GC
Cuts at: 2610 2610
Size: 5935
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2433 4060 4925 5223 2433
Size: 1627 865 298 3145
Fragments arranged by size:
3145 1627 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4598 882
Size: 617 3099 2219
Fragments arranged by size:
3099 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3726 493
Size: 711 2522 2702
Fragments arranged by size:
2702 2522 711
BsaBI GATnn'nnATC
Cuts at: 5929 5929
Size: 5935
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3742 4124 4759 275
Size: 53 83 186 936 2209 382 635 1451
Fragments arranged by size:
2209 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4368 4766 5040 5187 5598 4368
Size: 398 274 147 411 4705
Fragments arranged by size:
4705 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5710
Fragments arranged by size:
5710 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2695 1186
Size: 1509 4426
BseYI C'CCAG_C
Cuts at: 5138 5634 5138
Size: 496 5439
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5362
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3385 4146 5174 1540
Size: 275 186 1384 761 1028 2301
Fragments arranged by size:
2301 1384 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2144 2616 3996 4081 5152 2144
Size: 472 1380 85 1071 2927
Fragments arranged by size:
2927 1380 1071 472 85
BsmI GAATG_Cn'
Cuts at: 3446 3539 3446
Size: 93 5842
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2144 2598 2616 3184 3254 3996 4081
Size: 399 354 454 18 568 70 742 85
Cuts at: 4081 5152 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 568 454 399 354 85
70 18
BspEI T'CCGG_A
Cuts at: 5598 5598
Size: 5935
BspHI T'CATG_A
Cuts at: 3824 5554 3824
Size: 1730 4205
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3290 844
Size: 2446 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3822 1394
Size: 429 1999 3507
Fragments arranged by size:
3507 1999 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2159 2719 4430 4604 65
Size: 1183 911 560 1711 174 1396
Fragments arranged by size:
1711 1396 1183 911 560 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4579 4766 1700
Size: 110 2769 187 2869
Fragments arranged by size:
2869 2769 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5935
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5935
BssSI C'ACGA_G
Cuts at: 3995 5007 3995
Size: 1012 4923
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5935
BstBI TT'CG_AA
Cuts at: 4739 4739
Size: 5935
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5935
BstXI CCAn_nnnn'nTGG
Cuts at: 3202 3202
Size: 5935
BstYI r'GATC_y
Cuts at: 1972 2605 3265 3632 4017 4034 5486 5572 5584
Size: 633 660 367 385 17 1452 86 12
Cuts at: 5584 5930 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 660 633 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 3020 513
Size: 552 1955 3428
Fragments arranged by size:
3428 1955 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5196
Fragments arranged by size:
5196 683 56
BtsI GCAGTG_nn'
Cuts at: 2173 2938 3032 3451 4245 4265 2173
Size: 765 94 419 794 20 3843
Fragments arranged by size:
3843 794 765 419 94 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5935
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5900
DraI TTT'AAA
Cuts at: 2050 3239 2050
Size: 1189 4746
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4942 809
Size: 507 3626 1802
Fragments arranged by size:
3626 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2962 3382 4271
Size: 54 1425 50 275 18 1132 420 889
Cuts at: 4271 5900 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1132 889 420 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3382 1537
Size: 275 1570 4090
Fragments arranged by size:
4090 1570 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2401 3865 1353
Size: 1048 1464 3423
Fragments arranged by size:
3423 1464 1048
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4522 4896 5042 387
Size: 1064 138 2933 374 146 1280
Fragments arranged by size:
2933 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2614 2614
Size: 5935
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2742 2912 3998 4595 5382 5650 1239
Size: 393 1110 170 1086 597 787 268 1524
Fragments arranged by size:
1524 1110 1086 787 597 393 268 170
EcoNI CCTnn'n_nnAGG
Cuts at: 2050 2050
Size: 5935
EcoO109I rG'GnC_Cy
Cuts at: 3181 3181
Size: 5935
EcoRI G'AATT_C
Cuts at: 3244 3622 3244
Size: 378 5557
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2427 2427
Size: 5935
FspI TGC'GCA
Cuts at: 2165 4441 2165
Size: 2276 3659
HaeII r_GCGC'y
Cuts at: 1678 5082 1678
Size: 3404 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2690 2722 4576 4608 4650
Size: 32 135 32 1118 32 1854 32 42
Cuts at: 4650 4682 1373
Size: 32 2626
Fragments arranged by size:
2626 1854 1118 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2252 3279 3525 669
Size: 988 281 314 1027 246 3079
Fragments arranged by size:
3079 1027 988 314 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5935
HpaI GTT'AAC
Cuts at: 3525 3525
Size: 5935
KpnI G_GTAC'C
Cuts at: 2447 3260 2447
Size: 813 5122
MfeI C'AATT_G
Cuts at: 3534 3534
Size: 5935
MluI A'CGCG_T
Cuts at: 5811 5811
Size: 5935
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2931 5049 5233 1305
Size: 1626 2118 184 2007
Fragments arranged by size:
2118 2007 1626 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2964 10
Size: 54 1425 343 1132 2981
Fragments arranged by size:
2981 1425 1132 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3025 3893 4252 4411 518
Size: 648 1859 868 359 159 2042
Fragments arranged by size:
2042 1859 868 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3178 3351 4028 5176 5421 1606
Size: 1572 173 677 1148 245 2120
Fragments arranged by size:
2120 1572 1148 677 245 173
NcoI C'CATG_G
Cuts at: 513 1065 3020 513
Size: 552 1955 3428
Fragments arranged by size:
3428 1955 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5935
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5935
NotI GC'GGCC_GC
Cuts at: 3382 3382
Size: 5935
NsiI A_TGCA'T
Cuts at: 3050 3050
Size: 5935
NspI r_CATG'y
Cuts at: 1132 1229 2216 2435 3048 3293 4838 1132
Size: 97 987 219 613 245 1545 2229
Fragments arranged by size:
2229 1545 987 613 245 219 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5935
PciI A'CATG_T
Cuts at: 2431 4834 2431
Size: 2403 3532
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5935
PfoI T'CCnGG_A
Cuts at: 2526 2526
Size: 5935
PmeI GTTT'AAAC
Cuts at: 3239 3239
Size: 5935
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4004 4036 5517 5549 4004
Size: 32 1481 32 4390
Fragments arranged by size:
4390 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3171 1613
Size: 1558 4377
PsiI TTA'TAA
Cuts at: 2271 2538 3505 2271
Size: 267 967 4701
Fragments arranged by size:
4701 967 267
PspOMI G'GGCC_C
Cuts at: 3180 3180
Size: 5935
PstI C_TGCA'G
Cuts at: 830 3287 830
Size: 2457 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5935
SacI G_AGCT'C
Cuts at: 2616 2616
Size: 5935
SalI G'TCGA_C
Cuts at: 1936 3277 1936
Size: 1341 4594
SapI GCTCTTCn'nnn_
Cuts at: 2401 2401
Size: 5935
SbfI CC_TGCA'GG
Cuts at: 3287 3287
Size: 5935
ScaI AGT'ACT
Cuts at: 4183 5895 4183
Size: 1712 4223
SfcI C'TryA_G
Cuts at: 826 1045 3283 4418 5099 5290 826
Size: 219 2238 1135 681 191 1471
Fragments arranged by size:
2238 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5935
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5935
SmaI CCC'GGG
Cuts at: 1872 3262 1872
Size: 1390 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2448 4039 4940 5202 820
Size: 197 926 505 1591 901 262 1553
Fragments arranged by size:
1591 1553 926 901 505 262 197
SnaBI TAC'GTA
Cuts at: 493 3726 493
Size: 3233 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5935
SphI G_CATG'C
Cuts at: 1132 3293 1132
Size: 2161 3774
SspI AAT'ATT
Cuts at: 5 52 2030 2534 3859 5
Size: 47 1978 504 1325 2081
Fragments arranged by size:
2081 1978 1325 504 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5935
StyI C'CwwG_G
Cuts at: 513 1065 3020 3133 3374 513
Size: 552 1955 113 241 3074
Fragments arranged by size:
3074 1955 552 241 113
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2024 3208 3962 3979 4132 4317 4762
Size: 1255 444 1184 754 17 153 185 445
Cuts at: 4762 325
Size: 1498
Fragments arranged by size:
1498 1255 1184 754 445 444 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2457 2824 4181
Size: 274 80 33 51 1443 480 367 1357
Cuts at: 4181 5893 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1357 480 367 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2421 2453 5895 5927 2421
Size: 32 3442 32 2429
Fragments arranged by size:
3442 2429 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5935
XbaI T'CTAG_A
Cuts at: 2623 3271 2623
Size: 648 5287
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3153 3153
Size: 5935
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5935
XmaI C'CCGG_G
Cuts at: 1870 3260 1870
Size: 1390 4545
XmnI GAAnn'nnTTC
Cuts at: 4064 4064
Size: 5935
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3743 276
Size: 53 83 186 936 2209 2468
Fragments arranged by size:
2468 2209 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PpuMI PspXI PsrI PvuII RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI