Restriction map for
pFN21AB7967
(Circular) (Six-base) MAPSORT of: pFN21AB7967.seq Check: 511 from: 1 to: 5554
pFN21AB7967 5554 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3364 278
Size: 53 83 186 936 1828 2468
Fragments arranged by size:
2468 1828 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2897 1937
Size: 960 4594
Acc65I G'GTAC_C
Cuts at: 2875 2875
Size: 5554
AclI AA'CG_TT
Cuts at: 1563 2125 3681 4054 1563
Size: 562 1556 373 3063
Fragments arranged by size:
3063 1556 562 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2261 3617 5001 5269 1632
Size: 629 1356 1384 268 1917
Fragments arranged by size:
1917 1384 1356 629 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5357
AflIII A'CryG_T
Cuts at: 1203 4453 5430 1203
Size: 3250 977 1327
Fragments arranged by size:
3250 1327 977
AgeI A'CCGG_T
Cuts at: 2604 4385 2604
Size: 1781 3773
AhdI GACnn_n'nnGTC
Cuts at: 4283 4283
Size: 5554
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5554
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5554
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2115 2147 2115
Size: 32 5522
AlwNI CAG_nnn'CTG
Cuts at: 3297 4869 3297
Size: 1572 3982
ApaLI G'TGCA_C
Cuts at: 2051 2600 3611 4767 2051
Size: 549 1011 1156 2838
Fragments arranged by size:
2838 1156 1011 549
ApoI r'AATT_y
Cuts at: 1493 2171 2863 3089 3241 5350 1493
Size: 678 692 226 152 2109 1697
Fragments arranged by size:
2109 1697 692 678 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4108 160
Size: 872 3076 1606
Fragments arranged by size:
3076 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5554
AvaI C'yCGr_G
Cuts at: 1870 1943 2214 2281 2413 2501 2879 1870
Size: 73 271 67 132 88 378 4545
Fragments arranged by size:
4545 378 271 132 88 73 67
BamHI G'GATC_C
Cuts at: 2884 3251 2884
Size: 367 5187
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2607 2847 2875 4330 618
Size: 325 217 627 820 240 28 1455 1842
Fragments arranged by size:
1842 1455 820 627 325 240 217 28
BanII G_rGCy'C
Cuts at: 1391 2043 2145 2429 2873 1391
Size: 652 102 284 444 4072
Fragments arranged by size:
4072 652 444 284 102
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4583
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3745 3779 1903
Size: 34 1808 34 3678
Fragments arranged by size:
3678 1808 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3446 4656 3446
Size: 1210 4344
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5554
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4165 136
Size: 107 122 71 631 605 2493 1525
Fragments arranged by size:
2493 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2188 5549 2188
Size: 3361 2193
BlpI GC'TnA_GC
Cuts at: 2971 2971
Size: 5554
Bme1580I G_kGCm'C
Cuts at: 1790 2055 2502 2604 3615 4771 1790
Size: 265 447 102 1011 1156 2573
Fragments arranged by size:
2573 1156 1011 447 265 102
BmgBI CAC'GTC
Cuts at: 1318 2011 1318
Size: 693 4861
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3285 4243 442
Size: 416 53 83 399 24 1868 958 1753
Fragments arranged by size:
1868 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5554
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2530 2530
Size: 5554
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2027 2108 2117 2244 4214 1239
Size: 788 81 9 127 1970 2579
Fragments arranged by size:
2579 1970 788 127 81 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3679 4544 4842 3679
Size: 865 298 4391
Fragments arranged by size:
4391 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2051 2297 4217 882
Size: 617 552 246 1920 2219
Fragments arranged by size:
2219 1920 617 552 246
BsaAI yAC'GTr
Cuts at: 493 1204 3345 493
Size: 711 2141 2702
Fragments arranged by size:
2702 2141 711
BsaBI GATnn'nnATC
Cuts at: 5548 5548
Size: 5554
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3361 3743 4378 275
Size: 53 83 186 936 1828 382 635 1451
Fragments arranged by size:
1828 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2458 2604 3987 4385 4659 4806 5217 2458
Size: 146 1383 398 274 147 411 2795
Fragments arranged by size:
2795 1383 411 398 274 147 146
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2115 2145 1178
Size: 30 165 30 712 30 4587
Fragments arranged by size:
4587 712 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2105 2419 2608 1186
Size: 919 314 189 4132
Fragments arranged by size:
4132 919 314 189
BseYI C'CCAG_C
Cuts at: 4757 5253 4757
Size: 496 5058
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2044 2819 1163
Size: 573 308 775 3898
Fragments arranged by size:
3898 775 573 308
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3004 3765 4793 1540
Size: 275 186 1003 761 1028 2301
Fragments arranged by size:
2301 1028 1003 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2016 2043 2055 2429 2604 3615 3700 4771 2016
Size: 27 12 374 175 1011 85 1071 2799
Fragments arranged by size:
2799 1071 1011 374 175 85 27 12
BsmI GAATG_Cn'
Cuts at: 3065 3158 3065
Size: 93 5461
BsmBI CGTCTCn'nnnn_
Cuts at: 2088 2088
Size: 5554
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2016 2043 2055 2145 2429 2502 2604
Size: 399 226 27 12 90 284 73 102
Cuts at: 2604 2873 3615 3700 4771 1391
Size: 269 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 284 269 226 102
90 85 73 27 12
BspEI T'CCGG_A
Cuts at: 2458 5217 2458
Size: 2759 2795
BspHI T'CATG_A
Cuts at: 3443 5173 3443
Size: 1730 3824
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2117 2909 844
Size: 1273 792 3489
Fragments arranged by size:
3489 1273 792
BsrBI CCG'CTC
Cuts at: 1394 1823 3441 1394
Size: 429 1618 3507
Fragments arranged by size:
3507 1618 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4049 4223 65
Size: 1183 2801 174 1396
Fragments arranged by size:
2801 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2060 2604 4198 4385 1700
Size: 110 250 544 1594 187 2869
Fragments arranged by size:
2869 1594 544 250 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5554
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5554
BssSI C'ACGA_G
Cuts at: 2054 3614 4626 2054
Size: 1560 1012 2982
Fragments arranged by size:
2982 1560 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5554
BstBI TT'CG_AA
Cuts at: 4358 4358
Size: 5554
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5554
BstYI r'GATC_y
Cuts at: 1972 2188 2884 3251 3636 3653 5105 5191 5203
Size: 216 696 367 385 17 1452 86 12
Cuts at: 5203 5549 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 696 385 367 346 216 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2367 513
Size: 552 939 363 3700
Fragments arranged by size:
3700 939 552 363
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2586 487
Size: 683 56 1360 3455
Fragments arranged by size:
3455 1360 683 56
BtsI GCAGTG_nn'
Cuts at: 3070 3864 3884 3070
Size: 794 20 4740
Fragments arranged by size:
4740 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5554
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5519
DraI TTT'AAA
Cuts at: 2785 2858 2785
Size: 73 5481
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4561 809
Size: 507 3245 1802
Fragments arranged by size:
3245 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2370 3001 3890
Size: 54 1425 50 275 18 540 631 889
Cuts at: 3890 5519 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 631 540 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3001 1537
Size: 275 1189 4090
Fragments arranged by size:
4090 1189 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2837 3484 1353
Size: 1484 647 3423
Fragments arranged by size:
3423 1484 647
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4141 4515 4661 387
Size: 1064 138 2552 374 146 1280
Fragments arranged by size:
2552 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2041 2427 2041
Size: 386 5168
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2027 2108 2117 2244 2261 3617 4214
Size: 393 395 81 9 127 17 1356 597
Cuts at: 4214 5001 5269 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1356 787 597 395 393 268 127
81 17 9
EcoRI G'AATT_C
Cuts at: 2863 3241 2863
Size: 378 5176
EcoRV GAT'ATC
Cuts at: 2247 2247
Size: 5554
FspI TGC'GCA
Cuts at: 2531 4060 2531
Size: 1529 4025
HaeII r_GCGC'y
Cuts at: 1678 2078 4701 1678
Size: 400 2623 2531
Fragments arranged by size:
2623 2531 400
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4195 4227 4269 4301 1373
Size: 32 135 32 2623 32 42 32 2626
Fragments arranged by size:
2626 2623 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2745 2898 3144 669
Size: 988 281 807 153 246 3079
Fragments arranged by size:
3079 988 807 281 246 153
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5554
HpaI GTT'AAC
Cuts at: 2745 3144 2745
Size: 399 5155
KpnI G_GTAC'C
Cuts at: 2879 2879
Size: 5554
MfeI C'AATT_G
Cuts at: 3153 3153
Size: 5554
MluI A'CGCG_T
Cuts at: 5430 5430
Size: 5554
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2409 4668 4852 1305
Size: 1104 2259 184 2007
Fragments arranged by size:
2259 2007 1104 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3732
Fragments arranged by size:
3732 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3512 3871 4030 518
Size: 648 843 1503 359 159 2042
Fragments arranged by size:
2042 1503 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2535 2668 2970 3647 4795 5040 1606
Size: 929 133 302 677 1148 245 2120
Fragments arranged by size:
2120 1148 929 677 302 245 133
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4063
Fragments arranged by size:
4063 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5554
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5554
NotI GC'GGCC_GC
Cuts at: 3001 3001
Size: 5554
NspI r_CATG'y
Cuts at: 1132 1229 2912 4457 1132
Size: 97 1683 1545 2229
Fragments arranged by size:
2229 1683 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5554
PciI A'CATG_T
Cuts at: 4453 4453
Size: 5554
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5554
PfoI T'CCnGG_A
Cuts at: 2034 2034
Size: 5554
PmeI GTTT'AAAC
Cuts at: 2858 2858
Size: 5554
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2115 2147 3623 3655 5136 5168 2115
Size: 32 1476 32 1481 32 2501
Fragments arranged by size:
2501 1481 1476 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5554
PsiI TTA'TAA
Cuts at: 3124 3124
Size: 5554
PstI C_TGCA'G
Cuts at: 830 2627 2906 830
Size: 1797 279 3478
Fragments arranged by size:
3478 1797 279
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5554
SacI G_AGCT'C
Cuts at: 2043 2429 2043
Size: 386 5168
SalI G'TCGA_C
Cuts at: 1936 2896 1936
Size: 960 4594
SbfI CC_TGCA'GG
Cuts at: 2906 2906
Size: 5554
ScaI AGT'ACT
Cuts at: 2096 3802 5514 2096
Size: 1706 1712 2136
Fragments arranged by size:
2136 1712 1706
SfcI C'TryA_G
Cuts at: 826 1045 2623 2902 4037 4718 4909 826
Size: 219 1578 279 1135 681 191 1471
Fragments arranged by size:
1578 1471 1135 681 279 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5554
SgrAI Cr'CCGG_yG
Cuts at: 1700 2604 1700
Size: 904 4650
SmaI CCC'GGG
Cuts at: 1872 2503 2881 1872
Size: 631 378 4545
Fragments arranged by size:
4545 631 378
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3658 4559 4821 820
Size: 197 926 1715 901 262 1553
Fragments arranged by size:
1715 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3345 493
Size: 2852 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5554
SphI G_CATG'C
Cuts at: 1132 2912 1132
Size: 1780 3774
SspI AAT'ATT
Cuts at: 5 52 3478 5
Size: 47 3426 2081
Fragments arranged by size:
3426 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5554
StyI C'CwwG_G
Cuts at: 513 1065 2004 2993 513
Size: 552 939 989 3074
Fragments arranged by size:
3074 989 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3581 3598 3751 3936 4381 325
Size: 1255 2001 17 153 185 445 1498
Fragments arranged by size:
2001 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2094 3800 5512
Size: 274 80 33 51 1443 117 1706 1712
Cuts at: 5512 96
Size: 138
Fragments arranged by size:
1712 1706 1443 274 138 117 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2177 2209 2539 2571 5514 5546 2177
Size: 32 330 32 2943 32 2185
Fragments arranged by size:
2943 2185 330 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5554
XbaI T'CTAG_A
Cuts at: 2890 2890
Size: 5554
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2206 2206
Size: 5554
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5554
XmaI C'CCGG_G
Cuts at: 1870 2501 2879 1870
Size: 631 378 4545
Fragments arranged by size:
4545 631 378
XmnI GAAnn'nnTTC
Cuts at: 3683 3683
Size: 5554
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3362 276
Size: 53 83 186 936 1828 2468
Fragments arranged by size:
2468 1828 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI
EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI