Restriction map for
pFN21AB7966
(Circular) (Six-base) MAPSORT of: pFN21AB7966.seq Check: 8136 from: 1 to: 5908
pFN21AB7966 5908 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3718 278
Size: 53 83 186 936 2182 2468
Fragments arranged by size:
2468 2182 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2624 3251 1937
Size: 687 627 4594
Fragments arranged by size:
4594 687 627
Acc65I G'GTAC_C
Cuts at: 3229 3229
Size: 5908
AclI AA'CG_TT
Cuts at: 1563 4035 4408 1563
Size: 2472 373 3063
Fragments arranged by size:
3063 2472 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2565 3115 3173 3971 5355 5623 1632
Size: 933 550 58 798 1384 268 1917
Fragments arranged by size:
1917 1384 933 798 550 268 58
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5711
AflIII A'CryG_T
Cuts at: 1203 4807 5784 1203
Size: 3604 977 1327
Fragments arranged by size:
3604 1327 977
AgeI A'CCGG_T
Cuts at: 4739 4739
Size: 5908
AhdI GACnn_n'nnGTC
Cuts at: 4637 4637
Size: 5908
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5908
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5908
AlwNI CAG_nnn'CTG
Cuts at: 2423 3651 5223 2423
Size: 1228 1572 3108
Fragments arranged by size:
3108 1572 1228
ApaI G_GGCC'C
Cuts at: 2316 2316
Size: 5908
ApaLI G'TGCA_C
Cuts at: 3965 5121 3965
Size: 1156 4752
ApoI r'AATT_y
Cuts at: 1493 3217 3443 3595 5704 1493
Size: 1724 226 152 2109 1697
Fragments arranged by size:
2109 1724 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4462 160
Size: 872 3430 1606
Fragments arranged by size:
3430 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5908
AvaI C'yCGr_G
Cuts at: 1870 1943 2108 2878 3233 1870
Size: 73 165 770 355 4545
Fragments arranged by size:
4545 770 355 165 73
AvrII C'CTAG_G
Cuts at: 2845 2845
Size: 5908
BamHI G'GATC_C
Cuts at: 3238 3605 3238
Size: 367 5541
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2382 3229 4684 618
Size: 325 217 627 595 847 1455 1842
Fragments arranged by size:
1842 1455 847 627 595 325 217
BanII G_rGCy'C
Cuts at: 1391 2045 2063 2316 2756 3227 1391
Size: 654 18 253 440 471 4072
Fragments arranged by size:
4072 654 471 440 253 18
BbeI G_GCGC'C
Cuts at: 2386 2386
Size: 5908
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4937
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2522 2556 4099 4133 1903
Size: 34 585 34 1543 34 3678
Fragments arranged by size:
3678 1543 585 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3800 5010 3800
Size: 1210 4698
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5908
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2320 4519 136
Size: 107 122 71 631 605 648 2199 1525
Fragments arranged by size:
2199 1525 648 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5903 5903
Size: 5908
BlpI GC'TnA_GC
Cuts at: 3325 3325
Size: 5908
Bme1580I G_kGCm'C
Cuts at: 1790 2284 2316 3969 5125 1790
Size: 494 32 1653 1156 2573
Fragments arranged by size:
2573 1653 1156 494 32
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5908
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2113 3639 4597
Size: 416 53 83 399 24 696 1526 958
Cuts at: 4597 442
Size: 1753
Fragments arranged by size:
1753 1526 958 696 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5908
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4568 1239
Size: 3329 2579
Bpu10I CC'TnA_GC
Cuts at: 2488 2865 2488
Size: 377 5531
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4033 4898 5196 4033
Size: 865 298 4745
Fragments arranged by size:
4745 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2798 4571 882
Size: 617 1299 1773 2219
Fragments arranged by size:
2219 1773 1299 617
BsaAI yAC'GTr
Cuts at: 493 1204 3699 493
Size: 711 2495 2702
Fragments arranged by size:
2702 2495 711
BsaBI GATnn'nnATC
Cuts at: 2706 2774 5902 2706
Size: 68 3128 2712
Fragments arranged by size:
3128 2712 68
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2147 2383 3715 4097
Size: 53 83 186 936 614 236 1332 382
Cuts at: 4097 4732 275
Size: 635 1451
Fragments arranged by size:
1451 1332 936 635 614 382 236 186
83 53
BsaWI w'CCGG_w
Cuts at: 3112 4341 4739 5013 5160 5571 3112
Size: 1229 398 274 147 411 3449
Fragments arranged by size:
3449 1229 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5683
Fragments arranged by size:
5683 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2241 2367 2660 2816 3087 1186
Size: 1055 126 293 156 271 4007
Fragments arranged by size:
4007 1055 293 271 156 126
BseYI C'CCAG_C
Cuts at: 2188 2296 2309 2607 2828 5111 5607 2188
Size: 108 13 298 221 2283 496 2489
Fragments arranged by size:
2489 2283 496 298 221 108 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2552 1163
Size: 573 816 4519
Fragments arranged by size:
4519 816 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2324 2967 3358 4119 5147 1540
Size: 275 186 323 643 391 761 1028 2301
Fragments arranged by size:
2301 1028 761 643 391 323 275 186
BsiHKAI G_wGCw'C
Cuts at: 2126 2562 2756 3969 4054 5125 2126
Size: 436 194 1213 85 1071 2909
Fragments arranged by size:
2909 1213 1071 436 194 85
BsmI GAATG_Cn'
Cuts at: 3419 3512 3419
Size: 93 5815
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2045 2063 2126 2284 2316 2562 2756
Size: 399 255 18 63 158 32 246 194
Cuts at: 2756 3227 3969 4054 5125 1391
Size: 471 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 471 399 255 246 194
158 85 63 32 18
BspEI T'CCGG_A
Cuts at: 5571 5571
Size: 5908
BspHI T'CATG_A
Cuts at: 3797 5527 3797
Size: 1730 4178
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3263 844
Size: 2419 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3795 1394
Size: 429 1972 3507
Fragments arranged by size:
3507 1972 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4403 4577 65
Size: 1183 3155 174 1396
Fragments arranged by size:
3155 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2257 2319 4552 4739 1700
Size: 110 447 62 2233 187 2869
Fragments arranged by size:
2869 2233 447 187 110 62
BsrGI T'GTAC_A
Cuts at: 96 2822 96
Size: 2726 3182
BssHII G'CGCG_C
Cuts at: 1925 2261 1925
Size: 336 5572
BssSI C'ACGA_G
Cuts at: 3968 4980 3968
Size: 1012 4896
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5908
BstBI TT'CG_AA
Cuts at: 4712 4712
Size: 5908
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5908
BstXI CCAn_nnnn'nTGG
Cuts at: 2196 2196
Size: 5908
BstYI r'GATC_y
Cuts at: 1972 2701 3238 3605 3990 4007 5459 5545 5557
Size: 729 537 367 385 17 1452 86 12
Cuts at: 5557 5903 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 729 537 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2090 2133 2164 2195 2342 2728
Size: 552 939 86 43 31 31 147 386
Cuts at: 2728 513
Size: 3693
Fragments arranged by size:
3693 939 552 386 147 86 43 31
31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5169
Fragments arranged by size:
5169 683 56
BtsI GCAGTG_nn'
Cuts at: 2434 3424 4218 4238 2434
Size: 990 794 20 4104
Fragments arranged by size:
4104 990 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5908
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5873
DraI TTT'AAA
Cuts at: 3212 3212
Size: 5908
DraIII CAC_nnn'GTG
Cuts at: 3080 3080
Size: 5908
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4915 809
Size: 507 3599 1802
Fragments arranged by size:
3599 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2321 3003 3355
Size: 54 1425 50 275 18 491 682 352
Cuts at: 3355 4244 5873 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 682 491 352 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2321 3355 1537
Size: 275 509 1034 4090
Fragments arranged by size:
4090 1034 509 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3838 1353
Size: 2485 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4495 4869 5015 387
Size: 1064 138 2906 374 146 1280
Fragments arranged by size:
2906 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2754 2754
Size: 5908
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2565 3115 3173 3971 4568 5355 5623
Size: 393 933 550 58 798 597 787 268
Cuts at: 5623 1239
Size: 1524
Fragments arranged by size:
1524 933 798 787 597 550 393 268
58
EcoNI CCTnn'n_nnAGG
Cuts at: 2519 3122 2519
Size: 603 5305
EcoO109I rG'GnC_Cy
Cuts at: 2232 2313 2232
Size: 81 5827
EcoRI G'AATT_C
Cuts at: 3217 3595 3217
Size: 378 5530
FspI TGC'GCA
Cuts at: 4414 4414
Size: 5908
HaeII r_GCGC'y
Cuts at: 1678 2195 2386 5055 1678
Size: 517 191 2669 2531
Fragments arranged by size:
2669 2531 517 191
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4549 4581 4623 4655 1373
Size: 32 135 32 2977 32 42 32 2626
Fragments arranged by size:
2977 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3252 3498 669
Size: 988 281 1314 246 3079
Fragments arranged by size:
3079 1314 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5908
HpaI GTT'AAC
Cuts at: 3498 3498
Size: 5908
KasI G'GCGC_C
Cuts at: 2382 2382
Size: 5908
KpnI G_GTAC'C
Cuts at: 3233 3233
Size: 5908
MfeI C'AATT_G
Cuts at: 3507 3507
Size: 5908
MluI A'CGCG_T
Cuts at: 5784 5784
Size: 5908
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5022 5206 1305
Size: 3717 184 2007
Fragments arranged by size:
3717 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4086
Fragments arranged by size:
4086 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3866 4225 4384 518
Size: 648 2700 359 159 2042
Fragments arranged by size:
2700 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2166 2344 2483 2828 3206 3324 4001 5149
Size: 560 178 139 345 378 118 677 1148
Cuts at: 5149 5394 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 560 378 345 245 178
139 118
NaeI GCC'GGC
Cuts at: 2321 2321
Size: 5908
NarI GG'CG_CC
Cuts at: 2383 2383
Size: 5908
NcoI C'CATG_G
Cuts at: 513 1065 2004 2090 513
Size: 552 939 86 4331
Fragments arranged by size:
4331 939 552 86
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5908
NgoMIV G'CCGG_C
Cuts at: 2319 2319
Size: 5908
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5908
NotI GC'GGCC_GC
Cuts at: 3355 3355
Size: 5908
NspI r_CATG'y
Cuts at: 1132 1229 2683 3266 4811 1132
Size: 97 1454 583 1545 2229
Fragments arranged by size:
2229 1545 1454 583 97
PasI CC'CwG_GG
Cuts at: 1348 2356 1348
Size: 1008 4900
PciI A'CATG_T
Cuts at: 4807 4807
Size: 5908
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5908
PfoI T'CCnGG_A
Cuts at: 2391 2391
Size: 5908
PmeI GTTT'AAAC
Cuts at: 3212 3212
Size: 5908
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3977 4009 5490 5522 3977
Size: 32 1481 32 4363
Fragments arranged by size:
4363 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2232 2232
Size: 5908
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5908
PsiI TTA'TAA
Cuts at: 3478 3478
Size: 5908
PspOMI G'GGCC_C
Cuts at: 2312 2312
Size: 5908
PstI C_TGCA'G
Cuts at: 830 3178 3260 830
Size: 2348 82 3478
Fragments arranged by size:
3478 2348 82
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5908
PvuII CAG'CTG
Cuts at: 2828 3206 2828
Size: 378 5530
SacI G_AGCT'C
Cuts at: 2756 2756
Size: 5908
SacII CC_GC'GG
Cuts at: 2167 2345 2167
Size: 178 5730
SalI G'TCGA_C
Cuts at: 1936 3250 1936
Size: 1314 4594
SbfI CC_TGCA'GG
Cuts at: 3260 3260
Size: 5908
ScaI AGT'ACT
Cuts at: 4156 5868 4156
Size: 1712 4196
SfcI C'TryA_G
Cuts at: 826 1045 2630 3174 3256 4391 5072 5263 826
Size: 219 1585 544 82 1135 681 191 1471
Fragments arranged by size:
1585 1471 1135 681 544 219 191 82
SfiI GGCCn_nnn'nGGCC
Cuts at: 2320 2320
Size: 5908
SfoI GGC'GCC
Cuts at: 2384 2384
Size: 5908
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5908
SgrAI Cr'CCGG_yG
Cuts at: 1700 2257 1700
Size: 557 5351
SmaI CCC'GGG
Cuts at: 1872 2110 2880 3235 1872
Size: 238 770 355 4545
Fragments arranged by size:
4545 770 355 238
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4012 4913 5175 820
Size: 197 926 2069 901 262 1553
Fragments arranged by size:
2069 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3699 493
Size: 3206 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5908
SphI G_CATG'C
Cuts at: 1132 3266 1132
Size: 2134 3774
SspI AAT'ATT
Cuts at: 5 52 3832 5
Size: 47 3780 2081
Fragments arranged by size:
3780 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5908
StyI C'CwwG_G
Cuts at: 513 1065 2004 2090 2304 2463 2520 2845 3347
Size: 552 939 86 214 159 57 325 502
Cuts at: 3347 513
Size: 3074
Fragments arranged by size:
3074 939 552 502 325 214 159 86
57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2953 3935 3952 4105 4290 4735 325
Size: 1255 1373 982 17 153 185 445 1498
Fragments arranged by size:
1498 1373 1255 982 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2822 4154 5866
Size: 274 80 33 51 1443 845 1332 1712
Cuts at: 5866 96
Size: 138
Fragments arranged by size:
1712 1443 1332 845 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5868 5900 5868
Size: 32 5876
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5908
XbaI T'CTAG_A
Cuts at: 2797 3244 2797
Size: 447 5461
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2140 3131 2140
Size: 991 4917
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5908
XmaI C'CCGG_G
Cuts at: 1870 2108 2878 3233 1870
Size: 238 770 355 4545
Fragments arranged by size:
4545 770 355 238
XmnI GAAnn'nnTTC
Cuts at: 4037 4037
Size: 5908
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3716 276
Size: 53 83 186 936 2182 2468
Fragments arranged by size:
2468 2182 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SacII SalI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbvCI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI
NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI
SapI SexAI SrfI SwaI