Restriction map for
pFN21AB7957
(Circular) (Six-base) MAPSORT of: pFN21AB7957.seq Check: 2471 from: 1 to: 5488
pFN21AB7957 5488 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2129 2129
Size: 5488
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3298 278
Size: 53 83 186 936 1762 2468
Fragments arranged by size:
2468 1762 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2831 1937
Size: 894 4594
Acc65I G'GTAC_C
Cuts at: 2809 2809
Size: 5488
AclI AA'CG_TT
Cuts at: 1563 3615 3988 1563
Size: 2052 373 3063
Fragments arranged by size:
3063 2052 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2244 2706 3551 4935 5203 1632
Size: 612 462 845 1384 268 1917
Fragments arranged by size:
1917 1384 845 612 462 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5291
AflIII A'CryG_T
Cuts at: 1203 4387 5364 1203
Size: 3184 977 1327
Fragments arranged by size:
3184 1327 977
AgeI A'CCGG_T
Cuts at: 4319 4319
Size: 5488
AhdI GACnn_n'nnGTC
Cuts at: 4217 4217
Size: 5488
AleI CACnn'nnGTG
Cuts at: 1166 2114 1166
Size: 948 4540
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5488
AlwNI CAG_nnn'CTG
Cuts at: 2518 3231 4803 2518
Size: 713 1572 3203
Fragments arranged by size:
3203 1572 713
ApaLI G'TGCA_C
Cuts at: 3545 4701 3545
Size: 1156 4332
ApoI r'AATT_y
Cuts at: 1493 2797 3023 3175 5284 1493
Size: 1304 226 152 2109 1697
Fragments arranged by size:
2109 1697 1304 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4042 160
Size: 872 3010 1606
Fragments arranged by size:
3010 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5488
AvaI C'yCGr_G
Cuts at: 1870 1943 2168 2813 1870
Size: 73 225 645 4545
Fragments arranged by size:
4545 645 225 73
BamHI G'GATC_C
Cuts at: 2451 2818 3185 2451
Size: 367 367 4754
Fragments arranged by size:
4754 367 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2809 4264 618
Size: 325 217 627 1022 1455 1842
Fragments arranged by size:
1842 1455 1022 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2574 2807 1391
Size: 1183 233 4072
Fragments arranged by size:
4072 1183 233
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4517
BbvCI CC'TCA_GC
Cuts at: 2411 2738 2411
Size: 327 5161
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3679 3713 1903
Size: 34 1742 34 3678
Fragments arranged by size:
3678 1742 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3380 4590 3380
Size: 1210 4278
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5488
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4099 136
Size: 107 122 71 631 605 2427 1525
Fragments arranged by size:
2427 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5483 5483
Size: 5488
BlpI GC'TnA_GC
Cuts at: 2905 2905
Size: 5488
Bme1580I G_kGCm'C
Cuts at: 1790 3549 4705 1790
Size: 1759 1156 2573
Fragments arranged by size:
2573 1759 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5488
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3219 4177 442
Size: 416 53 83 399 24 1802 958 1753
Fragments arranged by size:
1802 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5488
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2788 2788
Size: 5488
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2367 2495 4148 1239
Size: 1128 128 1653 2579
Fragments arranged by size:
2579 1653 1128 128
Bpu10I CC'TnA_GC
Cuts at: 2300 2411 2738 2300
Size: 111 327 5050
Fragments arranged by size:
5050 327 111
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2071 2549 3613 4478 4776 2071
Size: 478 1064 865 298 2783
Fragments arranged by size:
2783 1064 865 478 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2729 4151 882
Size: 617 1230 1422 2219
Fragments arranged by size:
2219 1422 1230 617
BsaAI yAC'GTr
Cuts at: 493 1204 2263 3279 493
Size: 711 1059 1016 2702
Fragments arranged by size:
2702 1059 1016 711
BsaBI GATnn'nnATC
Cuts at: 5482 5482
Size: 5488
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3295 3677 4312 275
Size: 53 83 186 936 1762 382 635 1451
Fragments arranged by size:
1762 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2433 3921 4319 4593 4740 5151 2433
Size: 1488 398 274 147 411 2770
Fragments arranged by size:
2770 1488 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5263
Fragments arranged by size:
5263 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2717 1186
Size: 1531 3957
BseYI C'CCAG_C
Cuts at: 2018 2252 4691 5187 2018
Size: 234 2439 496 2319
Fragments arranged by size:
2439 2319 496 234
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2654 1163
Size: 573 918 3997
Fragments arranged by size:
3997 918 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2151 2938 3699 4727 1540
Size: 275 186 150 787 761 1028 2301
Fragments arranged by size:
2301 1028 787 761 275 186 150
BsiHKAI G_wGCw'C
Cuts at: 3549 3634 4705 3549
Size: 85 1071 4332
Fragments arranged by size:
4332 1071 85
BsmI GAATG_Cn'
Cuts at: 2999 3092 2999
Size: 93 5395
BsmBI CGTCTCn'nnnn_
Cuts at: 2194 2194
Size: 5488
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2574 2807 3549 3634 4705 1391
Size: 399 784 233 742 85 1071 2174
Fragments arranged by size:
2174 1071 784 742 399 233 85
BspEI T'CCGG_A
Cuts at: 5151 5151
Size: 5488
BspHI T'CATG_A
Cuts at: 3377 5107 3377
Size: 1730 3758
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2129 2843 844
Size: 1285 714 3489
Fragments arranged by size:
3489 1285 714
BsrBI CCG'CTC
Cuts at: 1394 1823 2599 3375 1394
Size: 429 776 776 3507
Fragments arranged by size:
3507 776 776 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3983 4157 65
Size: 1183 2735 174 1396
Fragments arranged by size:
2735 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4132 4319 1700
Size: 110 2322 187 2869
Fragments arranged by size:
2869 2322 187 110
BsrGI T'GTAC_A
Cuts at: 96 2478 96
Size: 2382 3106
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5488
BssSI C'ACGA_G
Cuts at: 3548 4560 3548
Size: 1012 4476
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5488
BstBI TT'CG_AA
Cuts at: 4292 4292
Size: 5488
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5488
BstXI CCAn_nnnn'nTGG
Cuts at: 2116 2652 2116
Size: 536 4952
BstYI r'GATC_y
Cuts at: 1972 2451 2818 3185 3570 3587 5039 5125 5137
Size: 479 367 367 385 17 1452 86 12
Cuts at: 5137 5483 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 479 385 367 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2085 2115 2278 513
Size: 552 939 81 30 163 3723
Fragments arranged by size:
3723 939 552 163 81 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4749
Fragments arranged by size:
4749 683 56
BtsI GCAGTG_nn'
Cuts at: 2455 3004 3798 3818 2455
Size: 549 794 20 4125
Fragments arranged by size:
4125 794 549 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5488
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5453
DraI TTT'AAA
Cuts at: 2792 2792
Size: 5488
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2218 2768 4495 809
Size: 507 902 550 1727 1802
Fragments arranged by size:
1802 1727 902 550 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2148 2265 2935
Size: 54 1425 50 275 18 318 117 670
Cuts at: 2935 3824 5453 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 670 318 275 117 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2148 2935 1537
Size: 275 336 787 4090
Fragments arranged by size:
4090 787 336 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2715 3418 1353
Size: 1362 703 3423
Fragments arranged by size:
3423 1362 703
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2396 2558 4075 4449 4595 387
Size: 1064 138 807 162 1517 374 146 1280
Fragments arranged by size:
1517 1280 1064 807 374 162 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2244 2367 2495 2706 3551 4148 4935
Size: 393 612 123 128 211 845 597 787
Cuts at: 4935 5203 1239
Size: 268 1524
Fragments arranged by size:
1524 845 787 612 597 393 268 211
128 123
EcoNI CCTnn'n_nnAGG
Cuts at: 2564 2564
Size: 5488
EcoO109I rG'GnC_Cy
Cuts at: 2075 2296 2352 2515 2075
Size: 221 56 163 5048
Fragments arranged by size:
5048 221 163 56
EcoRI G'AATT_C
Cuts at: 2797 3175 2797
Size: 378 5110
FspI TGC'GCA
Cuts at: 3994 3994
Size: 5488
HaeII r_GCGC'y
Cuts at: 1678 4635 1678
Size: 2957 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2516 2548 4129 4161 4203
Size: 32 135 32 944 32 1581 32 42
Cuts at: 4203 4235 1373
Size: 32 2626
Fragments arranged by size:
2626 1581 944 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2832 3078 669
Size: 988 281 894 246 3079
Fragments arranged by size:
3079 988 894 281 246
HindIII A'AGCT_T
Cuts at: 748 2047 748
Size: 1299 4189
HpaI GTT'AAC
Cuts at: 3078 3078
Size: 5488
KpnI G_GTAC'C
Cuts at: 2813 2813
Size: 5488
MfeI C'AATT_G
Cuts at: 3087 3087
Size: 5488
MluI A'CGCG_T
Cuts at: 5364 5364
Size: 5488
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4602 4786 1305
Size: 3297 184 2007
Fragments arranged by size:
3297 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2267 10
Size: 54 1425 343 435 3231
Fragments arranged by size:
3231 1425 435 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2114 3446 3805 3964 518
Size: 648 948 1332 359 159 2042
Fragments arranged by size:
2042 1332 948 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2904 3581 4729 4974 1606
Size: 1298 677 1148 245 2120
Fragments arranged by size:
2120 1298 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2085 2278 513
Size: 552 939 81 193 3723
Fragments arranged by size:
3723 939 552 193 81
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5488
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5488
NotI GC'GGCC_GC
Cuts at: 2935 2935
Size: 5488
NspI r_CATG'y
Cuts at: 1132 1229 2846 4391 1132
Size: 97 1617 1545 2229
Fragments arranged by size:
2229 1617 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2122 1348
Size: 774 4714
PciI A'CATG_T
Cuts at: 4387 4387
Size: 5488
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2518 1108
Size: 1410 4078
PfoI T'CCnGG_A
Cuts at: 2364 2727 2364
Size: 363 5125
PmeI GTTT'AAAC
Cuts at: 2792 2792
Size: 5488
PmlI CAC'GTG
Cuts at: 2263 2263
Size: 5488
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3557 3589 5070 5102 3557
Size: 32 1481 32 3943
Fragments arranged by size:
3943 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2075 2075
Size: 5488
PshAI GACnn'nnGTC
Cuts at: 1613 2186 1613
Size: 573 4915
PsiI TTA'TAA
Cuts at: 3058 3058
Size: 5488
PstI C_TGCA'G
Cuts at: 830 2586 2840 830
Size: 1756 254 3478
Fragments arranged by size:
3478 1756 254
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5488
SalI G'TCGA_C
Cuts at: 1936 2830 1936
Size: 894 4594
SbfI CC_TGCA'GG
Cuts at: 2840 2840
Size: 5488
ScaI AGT'ACT
Cuts at: 3736 5448 3736
Size: 1712 3776
SfcI C'TryA_G
Cuts at: 826 1045 2446 2582 2836 3971 4652 4843 826
Size: 219 1401 136 254 1135 681 191 1471
Fragments arranged by size:
1471 1401 1135 681 254 219 191 136
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5488
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5488
SmaI CCC'GGG
Cuts at: 1872 2815 1872
Size: 943 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2050 2564 3592 4493 4755 820
Size: 197 926 107 514 1028 901 262 1553
Fragments arranged by size:
1553 1028 926 901 514 262 197 107
SnaBI TAC'GTA
Cuts at: 493 3279 493
Size: 2786 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5488
SphI G_CATG'C
Cuts at: 1132 2846 1132
Size: 1714 3774
SspI AAT'ATT
Cuts at: 5 52 3412 5
Size: 47 3360 2081
Fragments arranged by size:
3360 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5488
StyI C'CwwG_G
Cuts at: 513 1065 2004 2065 2085 2278 2927 513
Size: 552 939 61 20 193 649 3074
Fragments arranged by size:
3074 939 649 552 193 61 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2271 3515 3532 3685 3870 4315 325
Size: 1255 691 1244 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1244 691 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2478 3734 5446
Size: 274 80 33 51 1443 501 1256 1712
Cuts at: 5446 96
Size: 138
Fragments arranged by size:
1712 1443 1256 501 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5448 5480 5448
Size: 32 5456
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5488
XbaI T'CTAG_A
Cuts at: 2824 2824
Size: 5488
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2260 2260
Size: 5488
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5488
XmaI C'CCGG_G
Cuts at: 1870 2813 1870
Size: 943 4545
XmnI GAAnn'nnTTC
Cuts at: 3617 3617
Size: 5488
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3296 276
Size: 53 83 186 936 1762 2468
Fragments arranged by size:
2468 1762 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI