Restriction map for
pFN21AB7951
(Circular) (Six-base) MAPSORT of: pFN21AB7951.seq Check: 3643 from: 1 to: 5470
pFN21AB7951 5470 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3280 278
Size: 53 83 186 936 1744 2468
Fragments arranged by size:
2468 1744 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2813 1937
Size: 876 4594
Acc65I G'GTAC_C
Cuts at: 2724 2791 2724
Size: 67 5403
AclI AA'CG_TT
Cuts at: 1563 3597 3970 1563
Size: 2034 373 3063
Fragments arranged by size:
3063 2034 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2715 3533 4917 5185 1632
Size: 1083 818 1384 268 1917
Fragments arranged by size:
1917 1384 1083 818 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5273
AflIII A'CryG_T
Cuts at: 1203 4369 5346 1203
Size: 3166 977 1327
Fragments arranged by size:
3166 1327 977
AgeI A'CCGG_T
Cuts at: 4301 4301
Size: 5470
AhdI GACnn_n'nnGTC
Cuts at: 4199 4199
Size: 5470
AleI CACnn'nnGTG
Cuts at: 1166 2342 1166
Size: 1176 4294
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5470
AlwNI CAG_nnn'CTG
Cuts at: 3213 4785 3213
Size: 1572 3898
ApaI G_GGCC'C
Cuts at: 2617 2617
Size: 5470
ApaLI G'TGCA_C
Cuts at: 3527 4683 3527
Size: 1156 4314
ApoI r'AATT_y
Cuts at: 1493 2779 3005 3157 5266 1493
Size: 1286 226 152 2109 1697
Fragments arranged by size:
2109 1697 1286 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4024 160
Size: 872 2992 1606
Fragments arranged by size:
2992 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5470
AvaI C'yCGr_G
Cuts at: 1870 1943 2275 2795 1870
Size: 73 332 520 4545
Fragments arranged by size:
4545 520 332 73
BamHI G'GATC_C
Cuts at: 2800 3167 2800
Size: 367 5103
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2168 2724 2791 4246 618
Size: 325 217 627 381 556 67 1455 1842
Fragments arranged by size:
1842 1455 627 556 381 325 217 67
BanII G_rGCy'C
Cuts at: 1391 2487 2617 2681 2699 2789 1391
Size: 1096 130 64 18 90 4072
Fragments arranged by size:
4072 1096 130 90 64 18
BbeI G_GCGC'C
Cuts at: 2172 2172
Size: 5470
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4499
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3661 3695 1903
Size: 34 1724 34 3678
Fragments arranged by size:
3678 1724 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3362 4572 3362
Size: 1210 4260
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5470
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4081 136
Size: 107 122 71 631 605 2409 1525
Fragments arranged by size:
2409 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2523 5465 2523
Size: 2942 2528
BlpI GC'TnA_GC
Cuts at: 2179 2887 2179
Size: 708 4762
Bme1580I G_kGCm'C
Cuts at: 1790 2121 2617 3531 4687 1790
Size: 331 496 914 1156 2573
Fragments arranged by size:
2573 1156 914 496 331
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5470
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3201 4159 442
Size: 416 53 83 399 24 1784 958 1753
Fragments arranged by size:
1784 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5470
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2327 2606 4130 1239
Size: 1088 279 1524 2579
Fragments arranged by size:
2579 1524 1088 279
Bpu10I CC'TnA_GC
Cuts at: 2680 2680
Size: 5470
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3595 4460 4758 3595
Size: 865 298 4307
Fragments arranged by size:
4307 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4133 882
Size: 617 2634 2219
Fragments arranged by size:
2634 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3261 493
Size: 711 2057 2702
Fragments arranged by size:
2702 2057 711
BsaBI GATnn'nnATC
Cuts at: 5464 5464
Size: 5470
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2169 3277 3659 4294
Size: 53 83 186 936 636 1108 382 635
Cuts at: 4294 275
Size: 1451
Fragments arranged by size:
1451 1108 936 636 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2436 3903 4301 4575 4722 5133 2436
Size: 1467 398 274 147 411 2773
Fragments arranged by size:
2773 1467 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2681 2711 1178
Size: 30 165 30 1278 30 3937
Fragments arranged by size:
3937 1278 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5470
BseYI C'CCAG_C
Cuts at: 2203 2708 4673 5169 2203
Size: 505 1965 496 2504
Fragments arranged by size:
2504 1965 505 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2285 1163
Size: 573 549 4348
Fragments arranged by size:
4348 573 549
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2201 2611 2920 3681 4709 1540
Size: 275 186 200 410 309 761 1028 2301
Fragments arranged by size:
2301 1028 761 410 309 275 200 186
BsiHKAI G_wGCw'C
Cuts at: 2339 2487 3531 3616 4687 2339
Size: 148 1044 85 1071 3122
Fragments arranged by size:
3122 1071 1044 148 85
BsmI GAATG_Cn'
Cuts at: 2981 3074 2981
Size: 93 5377
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2121 2339 2487 2617 2681 2699 2789
Size: 399 331 218 148 130 64 18 90
Cuts at: 2789 3531 3616 4687 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 331 218 148 130
90 85 64 18
BspEI T'CCGG_A
Cuts at: 5133 5133
Size: 5470
BspHI T'CATG_A
Cuts at: 3359 5089 3359
Size: 1730 3740
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2825 844
Size: 1981 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3357 1394
Size: 429 1534 3507
Fragments arranged by size:
3507 1534 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3965 4139 65
Size: 1183 2717 174 1396
Fragments arranged by size:
2717 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4114 4301 1700
Size: 110 2304 187 2869
Fragments arranged by size:
2869 2304 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5470
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5470
BssSI C'ACGA_G
Cuts at: 3530 4542 3530
Size: 1012 4458
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5470
BstBI TT'CG_AA
Cuts at: 4274 4274
Size: 5470
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5470
BstYI r'GATC_y
Cuts at: 1972 2385 2523 2800 3167 3552 3569 5021 5107
Size: 413 138 277 367 385 17 1452 86
Cuts at: 5107 5119 5465 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 413 385 367 346 277 138
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2113 513
Size: 552 1048 3870
Fragments arranged by size:
3870 1048 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4731
Fragments arranged by size:
4731 683 56
BtsI GCAGTG_nn'
Cuts at: 2986 3780 3800 2986
Size: 794 20 4656
Fragments arranged by size:
4656 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5470
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5435
DraI TTT'AAA
Cuts at: 2774 2774
Size: 5470
DraIII CAC_nnn'GTG
Cuts at: 2721 2721
Size: 5470
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4477 809
Size: 507 3161 1802
Fragments arranged by size:
3161 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2601 2917 3806
Size: 54 1425 50 275 18 771 316 889
Cuts at: 3806 5435 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 771 316 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2917 1537
Size: 275 1105 4090
Fragments arranged by size:
4090 1105 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2327 2668 3400 1353
Size: 974 341 732 3423
Fragments arranged by size:
3423 974 732 341
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2336 2495 4057 4431 4577 387
Size: 1064 138 747 159 1562 374 146 1280
Fragments arranged by size:
1562 1280 1064 747 374 159 146 138
EcoICRI GAG'CTC
Cuts at: 2485 2485
Size: 5470
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2327 2606 2715 3533 4130 4917 5185
Size: 393 695 279 109 818 597 787 268
Cuts at: 5185 1239
Size: 1524
Fragments arranged by size:
1524 818 787 695 597 393 279 268
109
EcoO109I rG'GnC_Cy
Cuts at: 2311 2614 2311
Size: 303 5167
EcoRI G'AATT_C
Cuts at: 2779 3157 2779
Size: 378 5092
FspI TGC'GCA
Cuts at: 3976 3976
Size: 5470
HaeII r_GCGC'y
Cuts at: 1678 2172 2286 4617 1678
Size: 494 114 2331 2531
Fragments arranged by size:
2531 2331 494 114
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4111 4143 4185 4217 1373
Size: 32 135 32 2539 32 42 32 2626
Fragments arranged by size:
2626 2539 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2221 2814 3060 669
Size: 988 281 283 593 246 3079
Fragments arranged by size:
3079 988 593 283 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5470
HpaI GTT'AAC
Cuts at: 3060 3060
Size: 5470
KasI G'GCGC_C
Cuts at: 2168 2168
Size: 5470
KpnI G_GTAC'C
Cuts at: 2728 2795 2728
Size: 67 5403
MfeI C'AATT_G
Cuts at: 3069 3069
Size: 5470
MluI A'CGCG_T
Cuts at: 5346 5346
Size: 5470
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4584 4768 1305
Size: 3279 184 2007
Fragments arranged by size:
3279 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2603 10
Size: 54 1425 343 771 2877
Fragments arranged by size:
2877 1425 771 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2342 3428 3787 3946 518
Size: 648 843 333 1086 359 159 2042
Fragments arranged by size:
2042 1086 843 648 359 333 159
MspA1I CmG'CkG
Cuts at: 1606 2203 2886 3563 4711 4956 1606
Size: 597 683 677 1148 245 2120
Fragments arranged by size:
2120 1148 683 677 597 245
NarI GG'CG_CC
Cuts at: 2169 2169
Size: 5470
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4918
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5470
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5470
NotI GC'GGCC_GC
Cuts at: 2917 2917
Size: 5470
NspI r_CATG'y
Cuts at: 1132 1229 2828 4373 1132
Size: 97 1599 1545 2229
Fragments arranged by size:
2229 1599 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2315 1348
Size: 967 4503
PciI A'CATG_T
Cuts at: 4369 4369
Size: 5470
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2767 1108
Size: 1659 3811
PfoI T'CCnGG_A
Cuts at: 2669 2669
Size: 5470
PmeI GTTT'AAAC
Cuts at: 2774 2774
Size: 5470
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2645 2677 3539 3571 5052 5084 2645
Size: 32 862 32 1481 32 3031
Fragments arranged by size:
3031 1481 862 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2311 2311
Size: 5470
PshAI GACnn'nnGTC
Cuts at: 1613 2516 1613
Size: 903 4567
PsiI TTA'TAA
Cuts at: 3040 3040
Size: 5470
PspOMI G'GGCC_C
Cuts at: 2613 2613
Size: 5470
PstI C_TGCA'G
Cuts at: 830 2822 830
Size: 1992 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5470
SacI G_AGCT'C
Cuts at: 2487 2487
Size: 5470
SalI G'TCGA_C
Cuts at: 1936 2812 1936
Size: 876 4594
SapI GCTCTTCn'nnn_
Cuts at: 2327 2327
Size: 5470
SbfI CC_TGCA'GG
Cuts at: 2822 2822
Size: 5470
ScaI AGT'ACT
Cuts at: 3718 5430 3718
Size: 1712 3758
SfcI C'TryA_G
Cuts at: 826 1045 2173 2818 3953 4634 4825 826
Size: 219 1128 645 1135 681 191 1471
Fragments arranged by size:
1471 1135 1128 681 645 219 191
SfoI GGC'GCC
Cuts at: 2170 2170
Size: 5470
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5470
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5470
SmaI CCC'GGG
Cuts at: 1872 2277 2797 1872
Size: 405 520 4545
Fragments arranged by size:
4545 520 405
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3574 4475 4737 820
Size: 197 926 1631 901 262 1553
Fragments arranged by size:
1631 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3261 493
Size: 2768 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5470
SphI G_CATG'C
Cuts at: 1132 2828 1132
Size: 1696 3774
SspI AAT'ATT
Cuts at: 5 52 3394 5
Size: 47 3342 2081
Fragments arranged by size:
3342 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5470
StyI C'CwwG_G
Cuts at: 513 1065 2909 513
Size: 552 1844 3074
Fragments arranged by size:
3074 1844 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2215 3497 3514 3667 3852 4297 325
Size: 1255 635 1282 17 153 185 445 1498
Fragments arranged by size:
1498 1282 1255 635 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3716 5428 96
Size: 274 80 33 51 1443 1739 1712 138
Fragments arranged by size:
1739 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5430 5462 5430
Size: 32 5438
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5470
XbaI T'CTAG_A
Cuts at: 2806 2806
Size: 5470
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5470
XmaI C'CCGG_G
Cuts at: 1870 2275 2795 1870
Size: 405 520 4545
Fragments arranged by size:
4545 520 405
XmnI GAAnn'nnTTC
Cuts at: 3599 3599
Size: 5470
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3278 276
Size: 53 83 186 936 1744 2468
Fragments arranged by size:
2468 1744 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI PvuII RsrII SacII SanDI SexAI
SfiI SrfI SwaI XcmI