Restriction map for
pFN21AB7945
(Circular) (Six-base) MAPSORT of: pFN21AB7945.seq Check: 3766 from: 1 to: 5878
pFN21AB7945 5878 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2491 3157 2491
Size: 666 5212
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3688 278
Size: 53 83 186 936 2152 2468
Fragments arranged by size:
2468 2152 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3221 1937
Size: 1284 4594
Acc65I G'GTAC_C
Cuts at: 2946 3199 2946
Size: 253 5625
AclI AA'CG_TT
Cuts at: 1563 4005 4378 1563
Size: 2442 373 3063
Fragments arranged by size:
3063 2442 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3941 5325 5593 1632
Size: 2309 1384 268 1917
Fragments arranged by size:
2309 1917 1384 268
AfeI AGC'GCT
Cuts at: 3124 3124
Size: 5878
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5681
AflIII A'CryG_T
Cuts at: 1203 2956 4777 5754 1203
Size: 1753 1821 977 1327
Fragments arranged by size:
1821 1753 1327 977
AgeI A'CCGG_T
Cuts at: 4709 4709
Size: 5878
AhdI GACnn_n'nnGTC
Cuts at: 4607 4607
Size: 5878
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5878
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5878
AlwNI CAG_nnn'CTG
Cuts at: 2157 2704 3621 5193 2157
Size: 547 917 1572 2842
Fragments arranged by size:
2842 1572 917 547
ApaLI G'TGCA_C
Cuts at: 3935 5091 3935
Size: 1156 4722
ApoI r'AATT_y
Cuts at: 1493 2205 3187 3413 3565 5674 1493
Size: 712 982 226 152 2109 1697
Fragments arranged by size:
2109 1697 982 712 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4432 160
Size: 872 3400 1606
Fragments arranged by size:
3400 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5878
AvaI C'yCGr_G
Cuts at: 1870 1943 2777 2942 3091 3132 3203 1870
Size: 73 834 165 149 41 71 4545
Fragments arranged by size:
4545 834 165 149 73 71 41
BamHI G'GATC_C
Cuts at: 2258 3208 3575 2258
Size: 950 367 4561
Fragments arranged by size:
4561 950 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2626 2946 3010 3199 4654
Size: 325 217 627 839 320 64 189 1455
Cuts at: 4654 618
Size: 1842
Fragments arranged by size:
1842 1455 839 627 325 320 217 189
64
BanII G_rGCy'C
Cuts at: 1391 2769 3197 1391
Size: 1378 428 4072
Fragments arranged by size:
4072 1378 428
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4907
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4069 4103 1903
Size: 34 2132 34 3678
Fragments arranged by size:
3678 2132 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3770 4980 3770
Size: 1210 4668
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5878
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2813 2846 4489
Size: 107 122 71 631 605 1141 33 1643
Cuts at: 4489 136
Size: 1525
Fragments arranged by size:
1643 1525 1141 631 605 122 107 71
33
BglII A'GATC_T
Cuts at: 5873 5873
Size: 5878
BlpI GC'TnA_GC
Cuts at: 3295 3295
Size: 5878
Bme1580I G_kGCm'C
Cuts at: 1790 3939 5095 1790
Size: 2149 1156 2573
Fragments arranged by size:
2573 2149 1156
BmgBI CAC'GTC
Cuts at: 1318 2568 2959 1318
Size: 1250 391 4237
Fragments arranged by size:
4237 1250 391
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3609 4567 442
Size: 416 53 83 399 24 2192 958 1753
Fragments arranged by size:
2192 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5878
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2678 2678
Size: 5878
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2152 2726 3097 4538 1239
Size: 913 574 371 1441 2579
Fragments arranged by size:
2579 1441 913 574 371
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4003 4868 5166 4003
Size: 865 298 4715
Fragments arranged by size:
4715 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4541 882
Size: 617 3042 2219
Fragments arranged by size:
3042 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3669 493
Size: 711 2465 2702
Fragments arranged by size:
2702 2465 711
BsaBI GATnn'nnATC
Cuts at: 5872 5872
Size: 5878
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3685 4067 4702 275
Size: 53 83 186 936 2152 382 635 1451
Fragments arranged by size:
2152 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2421 2623 2664 4311 4709 4983 5130 5541 2421
Size: 202 41 1647 398 274 147 411 2758
Fragments arranged by size:
2758 1647 411 398 274 202 147 41
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2085 2115 3056 3086 1178
Size: 30 165 30 682 30 941 30 3970
Fragments arranged by size:
3970 941 682 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2022 2073 3150 1186
Size: 836 51 1077 3914
Fragments arranged by size:
3914 1077 836 51
BseYI C'CCAG_C
Cuts at: 2458 2573 5081 5577 2458
Size: 115 2508 496 2759
Fragments arranged by size:
2759 2508 496 115
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5305
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2529 3328 4089 5117 1540
Size: 275 186 528 799 761 1028 2301
Fragments arranged by size:
2301 1028 799 761 528 275 186
BsiHKAI G_wGCw'C
Cuts at: 2519 2933 3939 4024 5095 2519
Size: 414 1006 85 1071 3302
Fragments arranged by size:
3302 1071 1006 414 85
BsmI GAATG_Cn'
Cuts at: 3389 3482 3389
Size: 93 5785
BsmBI CGTCTCn'nnnn_
Cuts at: 2595 2595
Size: 5878
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2519 2769 2933 3197 3939 4024 5095
Size: 399 729 250 164 264 742 85 1071
Cuts at: 5095 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 729 399 264 250 164
85
BspEI T'CCGG_A
Cuts at: 2421 2664 5541 2421
Size: 243 2877 2758
Fragments arranged by size:
2877 2758 243
BspHI T'CATG_A
Cuts at: 3767 5497 3767
Size: 1730 4148
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2205 2491 3157 3233 844
Size: 1361 286 666 76 3489
Fragments arranged by size:
3489 1361 666 286 76
BsrBI CCG'CTC
Cuts at: 1394 1823 2368 3765 1394
Size: 429 545 1397 3507
Fragments arranged by size:
3507 1397 545 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4373 4547 65
Size: 1183 3125 174 1396
Fragments arranged by size:
3125 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2352 2528 4522 4709 1700
Size: 110 542 176 1994 187 2869
Fragments arranged by size:
2869 1994 542 187 176 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5878
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5878
BssSI C'ACGA_G
Cuts at: 3938 4950 3938
Size: 1012 4866
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5878
BstBI TT'CG_AA
Cuts at: 4682 4682
Size: 5878
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5878
BstXI CCAn_nnnn'nTGG
Cuts at: 2290 2290
Size: 5878
BstYI r'GATC_y
Cuts at: 1972 2258 3208 3575 3960 3977 5429 5515 5527
Size: 286 950 367 385 17 1452 86 12
Cuts at: 5527 5873 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 950 385 367 346 286 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2188 2802 513
Size: 552 939 184 614 3589
Fragments arranged by size:
3589 939 614 552 184
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5139
Fragments arranged by size:
5139 683 56
BtsI GCAGTG_nn'
Cuts at: 2884 3159 3394 4188 4208 2884
Size: 275 235 794 20 4554
Fragments arranged by size:
4554 794 275 235 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5878
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2314 2349 529
Size: 35 1750 35 4058
Fragments arranged by size:
4058 1750 35 35
DraI TTT'AAA
Cuts at: 3182 3182
Size: 5878
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2503 4885 809
Size: 507 1187 2382 1802
Fragments arranged by size:
2382 1802 1187 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2096 2526 2635
Size: 54 1425 50 275 18 266 430 109
Cuts at: 2635 2847 3325 4214 5843 8
Size: 212 478 889 1629 43
Fragments arranged by size:
1629 1425 889 478 430 275 266 212
109 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2526 3325 1537
Size: 275 714 799 4090
Fragments arranged by size:
4090 799 714 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3808 1353
Size: 2455 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2105 4465 4839 4985 387
Size: 1064 138 516 2360 374 146 1280
Fragments arranged by size:
2360 1280 1064 516 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2152 2726 3097 3941 4538 5325 5593
Size: 393 520 574 371 844 597 787 268
Cuts at: 5593 1239
Size: 1524
Fragments arranged by size:
1524 844 787 597 574 520 393 371
268
EcoO109I rG'GnC_Cy
Cuts at: 2154 2154
Size: 5878
EcoRI G'AATT_C
Cuts at: 3187 3565 3187
Size: 378 5500
FspI TGC'GCA
Cuts at: 4384 4384
Size: 5878
HaeII r_GCGC'y
Cuts at: 1678 2580 3126 5025 1678
Size: 902 546 1899 2531
Fragments arranged by size:
2531 1899 902 546
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2085 2117 4519 4551 4593
Size: 32 135 32 513 32 2402 32 42
Cuts at: 4593 4625 1373
Size: 32 2626
Fragments arranged by size:
2626 2402 513 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3222 3468 669
Size: 988 281 1284 246 3079
Fragments arranged by size:
3079 1284 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5878
HpaI GTT'AAC
Cuts at: 3468 3468
Size: 5878
KpnI G_GTAC'C
Cuts at: 2950 3203 2950
Size: 253 5625
MfeI C'AATT_G
Cuts at: 3477 3477
Size: 5878
MluI A'CGCG_T
Cuts at: 5754 5754
Size: 5878
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2051 4992 5176 1305
Size: 746 2941 184 2007
Fragments arranged by size:
2941 2007 746 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2849 10
Size: 54 1425 343 1017 3039
Fragments arranged by size:
3039 1425 1017 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2145 2193 3836 4195 4354 518
Size: 648 979 48 1643 359 159 2042
Fragments arranged by size:
2042 1643 979 648 359 159 48
MspA1I CmG'CkG
Cuts at: 1606 2550 2704 2969 3294 3971 5119 5364 1606
Size: 944 154 265 325 677 1148 245 2120
Fragments arranged by size:
2120 1148 944 677 325 265 245 154
NaeI GCC'GGC
Cuts at: 2530 2530
Size: 5878
NcoI C'CATG_G
Cuts at: 513 1065 2004 2188 2802 513
Size: 552 939 184 614 3589
Fragments arranged by size:
3589 939 614 552 184
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5878
NgoMIV G'CCGG_C
Cuts at: 2528 2528
Size: 5878
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5878
NotI GC'GGCC_GC
Cuts at: 3325 3325
Size: 5878
NspI r_CATG'y
Cuts at: 1132 1229 3236 4781 1132
Size: 97 2007 1545 2229
Fragments arranged by size:
2229 2007 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5878
PciI A'CATG_T
Cuts at: 4777 4777
Size: 5878
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5878
PfoI T'CCnGG_A
Cuts at: 2103 3029 3074 2103
Size: 926 45 4907
Fragments arranged by size:
4907 926 45
PmeI GTTT'AAAC
Cuts at: 3182 3182
Size: 5878
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2624 2656 3947 3979 5460 5492 2624
Size: 32 1291 32 1481 32 3010
Fragments arranged by size:
3010 1481 1291 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5878
PsiI TTA'TAA
Cuts at: 3448 3448
Size: 5878
PstI C_TGCA'G
Cuts at: 830 3154 3230 830
Size: 2324 76 3478
Fragments arranged by size:
3478 2324 76
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5878
PvuII CAG'CTG
Cuts at: 2704 2704
Size: 5878
SalI G'TCGA_C
Cuts at: 1936 3220 1936
Size: 1284 4594
SbfI CC_TGCA'GG
Cuts at: 3230 3230
Size: 5878
ScaI AGT'ACT
Cuts at: 4126 5838 4126
Size: 1712 4166
SfcI C'TryA_G
Cuts at: 826 1045 3040 3150 3226 4361 5042 5233 826
Size: 219 1995 110 76 1135 681 191 1471
Fragments arranged by size:
1995 1471 1135 681 219 191 110 76
SfiI GGCCn_nnn'nGGCC
Cuts at: 2846 2846
Size: 5878
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5878
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5878
SmaI CCC'GGG
Cuts at: 1872 2944 3093 3205 1872
Size: 1072 149 112 4545
Fragments arranged by size:
4545 1072 149 112
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3982 4883 5145 820
Size: 197 926 2039 901 262 1553
Fragments arranged by size:
2039 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3669 493
Size: 3176 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5878
SphI G_CATG'C
Cuts at: 1132 3236 1132
Size: 2104 3774
SspI AAT'ATT
Cuts at: 5 52 3802 5
Size: 47 3750 2081
Fragments arranged by size:
3750 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5878
StyI C'CwwG_G
Cuts at: 513 1065 2004 2188 2802 3317 513
Size: 552 939 184 614 515 3074
Fragments arranged by size:
3074 939 614 552 515 184
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2296 2458 3905 3922 4075 4260 4705
Size: 1255 716 162 1447 17 153 185 445
Cuts at: 4705 325
Size: 1498
Fragments arranged by size:
1498 1447 1255 716 445 185 162 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2431 4124 5836
Size: 274 80 33 51 1443 454 1693 1712
Cuts at: 5836 96
Size: 138
Fragments arranged by size:
1712 1693 1443 454 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5838 5870 5838
Size: 32 5846
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5878
XbaI T'CTAG_A
Cuts at: 3214 3214
Size: 5878
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2284 2809 2284
Size: 525 5353
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5878
XmaI C'CCGG_G
Cuts at: 1870 2942 3091 3203 1870
Size: 1072 149 112 4545
Fragments arranged by size:
4545 1072 149 112
XmnI GAAnn'nnTTC
Cuts at: 2610 4007 2610
Size: 1397 4481
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3686 276
Size: 53 83 186 936 2152 2468
Fragments arranged by size:
2468 2152 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfoI SrfI SwaI