Restriction map for
pFN21AB7944
(Circular) (Six-base) MAPSORT of: pFN21AB7944.seq Check: 4518 from: 1 to: 5443
pFN21AB7944 5443 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2244 3253 278
Size: 53 83 186 936 708 1009 2468
Fragments arranged by size:
2468 1009 936 708 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2786 1937
Size: 849 4594
Acc65I G'GTAC_C
Cuts at: 2764 2764
Size: 5443
AclI AA'CG_TT
Cuts at: 1563 3570 3943 1563
Size: 2007 373 3063
Fragments arranged by size:
3063 2007 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3506 4890 5158 1632
Size: 1874 1384 268 1917
Fragments arranged by size:
1917 1874 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5246
AflIII A'CryG_T
Cuts at: 1203 4342 5319 1203
Size: 3139 977 1327
Fragments arranged by size:
3139 1327 977
AgeI A'CCGG_T
Cuts at: 4274 4274
Size: 5443
AhdI GACnn_n'nnGTC
Cuts at: 4172 4172
Size: 5443
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5443
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5443
AlwNI CAG_nnn'CTG
Cuts at: 2221 2255 3186 4758 2221
Size: 34 931 1572 2906
Fragments arranged by size:
2906 1572 931 34
ApaI G_GGCC'C
Cuts at: 2078 2078
Size: 5443
ApaLI G'TGCA_C
Cuts at: 3500 4656 3500
Size: 1156 4287
ApoI r'AATT_y
Cuts at: 1493 2752 2978 3130 5239 1493
Size: 1259 226 152 2109 1697
Fragments arranged by size:
2109 1697 1259 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3997 160
Size: 872 2965 1606
Fragments arranged by size:
2965 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5443
AvaI C'yCGr_G
Cuts at: 1870 1943 2071 2768 1870
Size: 73 128 697 4545
Fragments arranged by size:
4545 697 128 73
BamHI G'GATC_C
Cuts at: 2123 2773 3140 2123
Size: 650 367 4426
Fragments arranged by size:
4426 650 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2339 2360 2764 4219 618
Size: 325 217 627 552 21 404 1455 1842
Fragments arranged by size:
1842 1455 627 552 404 325 217 21
BanII G_rGCy'C
Cuts at: 1391 2059 2078 2162 2730 2762 1391
Size: 668 19 84 568 32 4072
Fragments arranged by size:
4072 668 568 84 32 19
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2493 928
Size: 971 594 3878
Fragments arranged by size:
3878 971 594
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2218 2252 3634 3668 1903
Size: 34 281 34 1382 34 3678
Fragments arranged by size:
3678 1382 281 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3335 4545 3335
Size: 1210 4233
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5443
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2034 4054 136
Size: 107 122 71 631 605 362 2020 1525
Fragments arranged by size:
2020 1525 631 605 362 122 107 71
BglII A'GATC_T
Cuts at: 2706 5438 2706
Size: 2732 2711
BlpI GC'TnA_GC
Cuts at: 2860 2860
Size: 5443
Bme1580I G_kGCm'C
Cuts at: 1790 2078 2365 3504 4660 1790
Size: 288 287 1139 1156 2573
Fragments arranged by size:
2573 1156 1139 288 287
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5443
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3174 4132 442
Size: 416 53 83 399 24 1757 958 1753
Fragments arranged by size:
1757 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5443
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2176 2176
Size: 5443
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2129 2186 2459 2723 4103 1239
Size: 890 57 273 264 1380 2579
Fragments arranged by size:
2579 1380 890 273 264 57
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3568 4433 4731 3568
Size: 865 298 4280
Fragments arranged by size:
4280 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4106 882
Size: 617 2607 2219
Fragments arranged by size:
2607 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3234 493
Size: 711 2030 2702
Fragments arranged by size:
2702 2030 711
BsaBI GATnn'nnATC
Cuts at: 5437 5437
Size: 5443
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2241 3250 3632 4267
Size: 53 83 186 936 708 1009 382 635
Cuts at: 4267 275
Size: 1451
Fragments arranged by size:
1451 1009 936 708 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2385 3876 4274 4548 4695 5106 2385
Size: 1491 398 274 147 411 2722
Fragments arranged by size:
2722 1491 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2579 2609 1178
Size: 30 165 30 1176 30 4012
Fragments arranged by size:
4012 1176 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2168 2183 2728 1186
Size: 982 15 545 3901
Fragments arranged by size:
3901 982 545 15
BseYI C'CCAG_C
Cuts at: 2060 2077 2332 4646 5142 2060
Size: 17 255 2314 496 2361
Fragments arranged by size:
2361 2314 496 255 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2275 1163
Size: 573 539 4331
Fragments arranged by size:
4331 573 539
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2893 3654 4682 1540
Size: 275 186 892 761 1028 2301
Fragments arranged by size:
2301 1028 892 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3504 3589 4660 3504
Size: 85 1071 4287
Fragments arranged by size:
4287 1071 85
BsmI GAATG_Cn'
Cuts at: 2954 3047 2954
Size: 93 5350
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2059 2078 2162 2365 2730 2762 3504
Size: 399 269 19 84 203 365 32 742
Cuts at: 3504 3589 4660 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 365 269 203 85
84 32 19
BspEI T'CCGG_A
Cuts at: 5106 5106
Size: 5443
BspHI T'CATG_A
Cuts at: 3332 5062 3332
Size: 1730 3713
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2652 2798 844
Size: 1808 146 3489
Fragments arranged by size:
3489 1808 146
BsrBI CCG'CTC
Cuts at: 1394 1823 2019 3330 1394
Size: 429 196 1311 3507
Fragments arranged by size:
3507 1311 429 196
BsrDI GCAATG_nn'
Cuts at: 65 1248 2203 3938 4112 65
Size: 1183 955 1735 174 1396
Fragments arranged by size:
1735 1396 1183 955 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4087 4274 1700
Size: 110 2277 187 2869
Fragments arranged by size:
2869 2277 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5443
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5443
BssSI C'ACGA_G
Cuts at: 2593 3503 4515 2593
Size: 910 1012 3521
Fragments arranged by size:
3521 1012 910
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2718 794
Size: 1924 3519
BstBI TT'CG_AA
Cuts at: 4247 4247
Size: 5443
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5443
BstXI CCAn_nnnn'nTGG
Cuts at: 2198 2198
Size: 5443
BstYI r'GATC_y
Cuts at: 1972 2123 2466 2706 2773 3140 3525 3542 4994
Size: 151 343 240 67 367 385 17 1452
Cuts at: 4994 5080 5092 5438 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 343 240 151
86 67 17 12
Bsu36I CC'TnA_GG
Cuts at: 2271 2271
Size: 5443
BtgI C'CryG_G
Cuts at: 513 1065 2691 513
Size: 552 1626 3265
Fragments arranged by size:
3265 1626 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4704
Fragments arranged by size:
4704 683 56
BtsI GCAGTG_nn'
Cuts at: 2500 2959 3753 3773 2500
Size: 459 794 20 4170
Fragments arranged by size:
4170 794 459 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5443
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5408
DraI TTT'AAA
Cuts at: 2747 2747
Size: 5443
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2240 2495 4450 809
Size: 507 924 255 1955 1802
Fragments arranged by size:
1955 1802 924 507 255
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2020 2317 2890
Size: 54 1425 50 275 18 190 297 573
Cuts at: 2890 3779 5408 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 573 297 275 190 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2890 1537
Size: 275 1078 4090
Fragments arranged by size:
4090 1078 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2426 3373 1353
Size: 1073 947 3423
Fragments arranged by size:
3423 1073 947
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4030 4404 4550 387
Size: 1064 138 2441 374 146 1280
Fragments arranged by size:
2441 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2129 2186 2459 2723 3506 4103 4890
Size: 393 497 57 273 264 783 597 787
Cuts at: 4890 5158 1239
Size: 268 1524
Fragments arranged by size:
1524 787 783 597 497 393 273 268
264 57
EcoNI CCTnn'n_nnAGG
Cuts at: 2168 2168
Size: 5443
EcoO109I rG'GnC_Cy
Cuts at: 2218 2218
Size: 5443
EcoRI G'AATT_C
Cuts at: 2752 3130 2752
Size: 378 5065
FspI TGC'GCA
Cuts at: 3949 3949
Size: 5443
HaeII r_GCGC'y
Cuts at: 1678 2029 4590 1678
Size: 351 2561 2531
Fragments arranged by size:
2561 2531 351
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4084 4116 4158 4190 1373
Size: 32 135 32 2512 32 42 32 2626
Fragments arranged by size:
2626 2512 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2787 3033 669
Size: 988 281 849 246 3079
Fragments arranged by size:
3079 988 849 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5443
HpaI GTT'AAC
Cuts at: 3033 3033
Size: 5443
KpnI G_GTAC'C
Cuts at: 2768 2768
Size: 5443
MfeI C'AATT_G
Cuts at: 3042 3042
Size: 5443
MluI A'CGCG_T
Cuts at: 5319 5319
Size: 5443
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4557 4741 1305
Size: 3252 184 2007
Fragments arranged by size:
3252 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2319 10
Size: 54 1425 343 487 3134
Fragments arranged by size:
3134 1425 487 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3401 3760 3919 518
Size: 648 2235 359 159 2042
Fragments arranged by size:
2235 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2693 2859 3536 4684 4929 1606
Size: 1087 166 677 1148 245 2120
Fragments arranged by size:
2120 1148 1087 677 245 166
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4891
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5443
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5443
NotI GC'GGCC_GC
Cuts at: 2890 2890
Size: 5443
NspI r_CATG'y
Cuts at: 1132 1229 2265 2801 4346 1132
Size: 97 1036 536 1545 2229
Fragments arranged by size:
2229 1545 1036 536 97
PasI CC'CwG_GG
Cuts at: 1348 2215 2365 1348
Size: 867 150 4426
Fragments arranged by size:
4426 867 150
PciI A'CATG_T
Cuts at: 4342 4342
Size: 5443
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2221 1108
Size: 1113 4330
PfoI T'CCnGG_A
Cuts at: 2553 2670 2553
Size: 117 5326
PmeI GTTT'AAAC
Cuts at: 2747 2747
Size: 5443
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3512 3544 5025 5057 3512
Size: 32 1481 32 3898
Fragments arranged by size:
3898 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5443
PsiI TTA'TAA
Cuts at: 3013 3013
Size: 5443
PspOMI G'GGCC_C
Cuts at: 2074 2074
Size: 5443
PstI C_TGCA'G
Cuts at: 830 2582 2795 830
Size: 1752 213 3478
Fragments arranged by size:
3478 1752 213
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5443
SacII CC_GC'GG
Cuts at: 2694 2694
Size: 5443
SalI G'TCGA_C
Cuts at: 1936 2785 1936
Size: 849 4594
SbfI CC_TGCA'GG
Cuts at: 2795 2795
Size: 5443
ScaI AGT'ACT
Cuts at: 3691 5403 3691
Size: 1712 3731
SexAI A'CCwGG_T
Cuts at: 2010 2010
Size: 5443
SfcI C'TryA_G
Cuts at: 826 1045 2578 2791 3926 4607 4798 826
Size: 219 1533 213 1135 681 191 1471
Fragments arranged by size:
1533 1471 1135 681 219 213 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5443
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5443
SmaI CCC'GGG
Cuts at: 1872 2073 2770 1872
Size: 201 697 4545
Fragments arranged by size:
4545 697 201
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3547 4448 4710 820
Size: 197 926 1604 901 262 1553
Fragments arranged by size:
1604 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3234 493
Size: 2741 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5443
SphI G_CATG'C
Cuts at: 1132 2801 1132
Size: 1669 3774
SspI AAT'ATT
Cuts at: 5 52 3367 5
Size: 47 3315 2081
Fragments arranged by size:
3315 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5443
StyI C'CwwG_G
Cuts at: 513 1065 2191 2283 2376 2882 513
Size: 552 1126 92 93 506 3074
Fragments arranged by size:
3074 1126 552 506 93 92
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2357 2418 3470 3487 3640 3825 4270
Size: 1255 777 61 1052 17 153 185 445
Cuts at: 4270 325
Size: 1498
Fragments arranged by size:
1498 1255 1052 777 445 185 153 61
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3689 5401 96
Size: 274 80 33 51 1443 1712 1712 138
Fragments arranged by size:
1712 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2579 2611 5403 5435 2579
Size: 32 2792 32 2587
Fragments arranged by size:
2792 2587 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5443
XbaI T'CTAG_A
Cuts at: 2779 2779
Size: 5443
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2030 2030
Size: 5443
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5443
XmaI C'CCGG_G
Cuts at: 1870 2071 2768 1870
Size: 201 697 4545
Fragments arranged by size:
4545 697 201
XmnI GAAnn'nnTTC
Cuts at: 3572 3572
Size: 5443
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2242 3251 276
Size: 53 83 186 936 708 1009 2468
Fragments arranged by size:
2468 1009 936 708 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI SacII SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII
SacI SanDI SapI SfiI SfoI SrfI SwaI