Restriction map for
pFN21AB7940
(Circular) (Six-base) MAPSORT of: pFN21AB7940.seq Check: 3799 from: 1 to: 5425
pFN21AB7940 5425 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3235 278
Size: 53 83 186 936 1699 2468
Fragments arranged by size:
2468 1699 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2768 1937
Size: 831 4594
Acc65I G'GTAC_C
Cuts at: 2746 2746
Size: 5425
AclI AA'CG_TT
Cuts at: 1563 3552 3925 1563
Size: 1989 373 3063
Fragments arranged by size:
3063 1989 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2051 2357 3488 4872 5140 1632
Size: 419 306 1131 1384 268 1917
Fragments arranged by size:
1917 1384 1131 419 306 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5228
AflIII A'CryG_T
Cuts at: 1203 4324 5301 1203
Size: 3121 977 1327
Fragments arranged by size:
3121 1327 977
AgeI A'CCGG_T
Cuts at: 4256 4256
Size: 5425
AhdI GACnn_n'nnGTC
Cuts at: 4154 4154
Size: 5425
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5425
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5425
AlwNI CAG_nnn'CTG
Cuts at: 3168 4740 3168
Size: 1572 3853
ApaLI G'TGCA_C
Cuts at: 2516 3482 4638 2516
Size: 966 1156 3303
Fragments arranged by size:
3303 1156 966
ApoI r'AATT_y
Cuts at: 1493 2734 2960 3112 5221 1493
Size: 1241 226 152 2109 1697
Fragments arranged by size:
2109 1697 1241 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3979 160
Size: 872 2947 1606
Fragments arranged by size:
2947 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5425
AvaI C'yCGr_G
Cuts at: 1870 1943 2424 2750 1870
Size: 73 481 326 4545
Fragments arranged by size:
4545 481 326 73
BamHI G'GATC_C
Cuts at: 2755 3122 2755
Size: 367 5058
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2746 4201 618
Size: 325 217 627 959 1455 1842
Fragments arranged by size:
1842 1455 959 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2744 1391
Size: 1353 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2171 2460 928
Size: 971 272 289 3893
Fragments arranged by size:
3893 971 289 272
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3616 3650 1903
Size: 34 1679 34 3678
Fragments arranged by size:
3678 1679 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3317 4527 3317
Size: 1210 4215
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5425
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4036 136
Size: 107 122 71 631 605 2364 1525
Fragments arranged by size:
2364 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2332 5420 2332
Size: 3088 2337
BlpI GC'TnA_GC
Cuts at: 2842 2842
Size: 5425
Bme1580I G_kGCm'C
Cuts at: 1790 2520 3486 4642 1790
Size: 730 966 1156 2573
Fragments arranged by size:
2573 1156 966 730
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5425
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3156 4114 442
Size: 416 53 83 399 24 1739 958 1753
Fragments arranged by size:
1753 1739 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5425
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2170 2170
Size: 5425
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2160 4085 1239
Size: 921 1925 2579
Fragments arranged by size:
2579 1925 921
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3550 4415 4713 3550
Size: 865 298 4262
Fragments arranged by size:
4262 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2590 4088 882
Size: 617 1091 1498 2219
Fragments arranged by size:
2219 1498 1091 617
BsaAI yAC'GTr
Cuts at: 493 1204 2565 3216 493
Size: 711 1361 651 2702
Fragments arranged by size:
2702 1361 711 651
BsaBI GATnn'nnATC
Cuts at: 2132 5419 2132
Size: 3287 2138
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3232 3614 4249 275
Size: 53 83 186 936 1699 382 635 1451
Fragments arranged by size:
1699 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2372 3858 4256 4530 4677 5088 2372
Size: 1486 398 274 147 411 2709
Fragments arranged by size:
2709 1486 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2158 2188 2570 2600 1178
Size: 30 165 30 755 30 382 30 4003
Fragments arranged by size:
4003 755 382 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5425
BseYI C'CCAG_C
Cuts at: 4628 5124 4628
Size: 496 4929
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2273 1163
Size: 573 537 4315
Fragments arranged by size:
4315 573 537
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2013 2875 3636 4664 1540
Size: 275 186 12 862 761 1028 2301
Fragments arranged by size:
2301 1028 862 761 275 186 12
BsiHKAI G_wGCw'C
Cuts at: 2031 2520 3486 3571 4642 2031
Size: 489 966 85 1071 2814
Fragments arranged by size:
2814 1071 966 489 85
BsmI GAATG_Cn'
Cuts at: 2936 3029 2936
Size: 93 5332
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2031 2520 2744 3486 3571 4642 1391
Size: 399 241 489 224 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 489 399 241 224 85
BspEI T'CCGG_A
Cuts at: 2372 5088 2372
Size: 2716 2709
BspHI T'CATG_A
Cuts at: 3314 5044 3314
Size: 1730 3695
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2780 844
Size: 1936 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3312 1394
Size: 429 1489 3507
Fragments arranged by size:
3507 1489 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3920 4094 65
Size: 1183 2672 174 1396
Fragments arranged by size:
2672 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4069 4256 1700
Size: 110 2259 187 2869
Fragments arranged by size:
2869 2259 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5425
BssHII G'CGCG_C
Cuts at: 1925 2318 1925
Size: 393 5032
BssSI C'ACGA_G
Cuts at: 3485 4497 3485
Size: 1012 4413
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5425
BstBI TT'CG_AA
Cuts at: 2296 4229 2296
Size: 1933 3492
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5425
BstXI CCAn_nnnn'nTGG
Cuts at: 2023 2634 2023
Size: 611 4814
BstYI r'GATC_y
Cuts at: 1972 2205 2332 2755 3122 3507 3524 4976 5062
Size: 233 127 423 367 385 17 1452 86
Cuts at: 5062 5074 5420 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 423 385 367 346 233 127
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2157 2544 2693 513
Size: 552 939 153 387 149 3245
Fragments arranged by size:
3245 939 552 387 153 149
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4686
Fragments arranged by size:
4686 683 56
BtsI GCAGTG_nn'
Cuts at: 2362 2941 3735 3755 2362
Size: 579 794 20 4032
Fragments arranged by size:
4032 794 579 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5425
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5390
DraI TTT'AAA
Cuts at: 2729 2729
Size: 5425
DraIII CAC_nnn'GTG
Cuts at: 2699 2699
Size: 5425
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4432 809
Size: 507 3116 1802
Fragments arranged by size:
3116 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2259 2872 3761
Size: 54 1425 50 275 18 429 613 889
Cuts at: 3761 5390 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 613 429 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2872 1537
Size: 275 1060 4090
Fragments arranged by size:
4090 1060 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3355 1353
Size: 2002 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4012 4386 4532 387
Size: 1064 138 2423 374 146 1280
Fragments arranged by size:
2423 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2051 2160 2357 3488 4085 4872 5140
Size: 393 419 109 197 1131 597 787 268
Cuts at: 5140 1239
Size: 1524
Fragments arranged by size:
1524 1131 787 597 419 393 268 197
109
EcoO109I rG'GnC_Cy
Cuts at: 2421 2421
Size: 5425
EcoRI G'AATT_C
Cuts at: 2734 3112 2734
Size: 378 5047
FspI TGC'GCA
Cuts at: 3931 3931
Size: 5425
HaeII r_GCGC'y
Cuts at: 1678 2197 4572 1678
Size: 519 2375 2531
Fragments arranged by size:
2531 2375 519
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2158 2190 4066 4098 4140
Size: 32 135 32 586 32 1876 32 42
Cuts at: 4140 4172 1373
Size: 32 2626
Fragments arranged by size:
2626 1876 586 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2769 3015 669
Size: 988 281 831 246 3079
Fragments arranged by size:
3079 988 831 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5425
HpaI GTT'AAC
Cuts at: 3015 3015
Size: 5425
KpnI G_GTAC'C
Cuts at: 2750 2750
Size: 5425
MfeI C'AATT_G
Cuts at: 2628 3024 2628
Size: 396 5029
MluI A'CGCG_T
Cuts at: 5301 5301
Size: 5425
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4539 4723 1305
Size: 3234 184 2007
Fragments arranged by size:
3234 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2261 10
Size: 54 1425 343 429 3174
Fragments arranged by size:
3174 1425 429 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2543 3383 3742 3901 518
Size: 648 1377 840 359 159 2042
Fragments arranged by size:
2042 1377 840 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2083 2257 2841 3518 4666 4911 1606
Size: 477 174 584 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 584 477 245 174
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3934
Fragments arranged by size:
3934 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5425
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5425
NotI GC'GGCC_GC
Cuts at: 2872 2872
Size: 5425
NspI r_CATG'y
Cuts at: 1132 1229 2542 2783 4328 1132
Size: 97 1313 241 1545 2229
Fragments arranged by size:
2229 1545 1313 241 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5425
PciI A'CATG_T
Cuts at: 4324 4324
Size: 5425
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2188 1108
Size: 1080 4345
PfoI T'CCnGG_A
Cuts at: 2703 2703
Size: 5425
PmeI GTTT'AAAC
Cuts at: 2729 2729
Size: 5425
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3494 3526 5007 5039 3494
Size: 32 1481 32 3880
Fragments arranged by size:
3880 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2421 2421
Size: 5425
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5425
PsiI TTA'TAA
Cuts at: 2995 2995
Size: 5425
PstI C_TGCA'G
Cuts at: 830 2146 2202 2777 830
Size: 1316 56 575 3478
Fragments arranged by size:
3478 1316 575 56
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5425
PvuII CAG'CTG
Cuts at: 2083 2257 2083
Size: 174 5251
SalI G'TCGA_C
Cuts at: 1936 2767 1936
Size: 831 4594
SanDI GG'GwC_CC
Cuts at: 2421 2421
Size: 5425
SbfI CC_TGCA'GG
Cuts at: 2777 2777
Size: 5425
ScaI AGT'ACT
Cuts at: 3673 5385 3673
Size: 1712 3713
SexAI A'CCwGG_T
Cuts at: 2580 2580
Size: 5425
SfcI C'TryA_G
Cuts at: 826 1045 2142 2198 2218 2773 3908 4589 4780
Size: 219 1097 56 20 555 1135 681 191
Cuts at: 4780 826
Size: 1471
Fragments arranged by size:
1471 1135 1097 681 555 219 191 56
20
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5425
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5425
SmaI CCC'GGG
Cuts at: 1872 2426 2752 1872
Size: 554 326 4545
Fragments arranged by size:
4545 554 326
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3529 4430 4692 820
Size: 197 926 1586 901 262 1553
Fragments arranged by size:
1586 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3216 493
Size: 2723 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5425
SphI G_CATG'C
Cuts at: 1132 2783 1132
Size: 1651 3774
SspI AAT'ATT
Cuts at: 5 52 3349 5
Size: 47 3297 2081
Fragments arranged by size:
3297 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5425
StyI C'CwwG_G
Cuts at: 513 1065 2004 2864 513
Size: 552 939 860 3074
Fragments arranged by size:
3074 939 860 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3452 3469 3622 3807 4252 325
Size: 1255 1872 17 153 185 445 1498
Fragments arranged by size:
1872 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2019 2148 3671
Size: 274 80 33 51 1443 42 129 1523
Cuts at: 3671 5383 96
Size: 1712 138
Fragments arranged by size:
1712 1523 1443 274 138 129 80 51
42 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2570 2602 2685 2717 5385 5417 2570
Size: 32 83 32 2668 32 2578
Fragments arranged by size:
2668 2578 83 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2170 1316
Size: 854 4571
XbaI T'CTAG_A
Cuts at: 2761 2761
Size: 5425
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2588 2588
Size: 5425
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5425
XmaI C'CCGG_G
Cuts at: 1870 2424 2750 1870
Size: 554 326 4545
Fragments arranged by size:
4545 554 326
XmnI GAAnn'nnTTC
Cuts at: 3554 3554
Size: 5425
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3233 276
Size: 53 83 186 936 1699 2468
Fragments arranged by size:
2468 1699 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SapI SfiI SfoI SrfI SwaI