Restriction map for
pFN21AB7931
(Circular) (Six-base) MAPSORT of: pFN21AB7931.seq Check: 317 from: 1 to: 5800
pFN21AB7931 5800 bp
With 182 enzymes: SgfI *
January 30, 2014 17:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3610 278
Size: 53 83 186 936 2074 2468
Fragments arranged by size:
2468 2074 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3143 1937
Size: 1206 4594
Acc65I G'GTAC_C
Cuts at: 3121 3121
Size: 5800
AclI AA'CG_TT
Cuts at: 1563 3927 4300 1563
Size: 2364 373 3063
Fragments arranged by size:
3063 2364 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2466 3863 5247 5515 1632
Size: 834 1397 1384 268 1917
Fragments arranged by size:
1917 1397 1384 834 268
AfeI AGC'GCT
Cuts at: 2678 2678
Size: 5800
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5603
AflIII A'CryG_T
Cuts at: 1203 4699 5676 1203
Size: 3496 977 1327
Fragments arranged by size:
3496 1327 977
AgeI A'CCGG_T
Cuts at: 4631 4631
Size: 5800
AhdI GACnn_n'nnGTC
Cuts at: 2440 4529 2440
Size: 2089 3711
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5800
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2762 1184
Size: 1578 4222
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2309 2341 2309
Size: 32 5768
AlwNI CAG_nnn'CTG
Cuts at: 3543 5115 3543
Size: 1572 4228
ApaI G_GGCC'C
Cuts at: 2201 2236 2345 3075 2201
Size: 35 109 730 4926
Fragments arranged by size:
4926 730 109 35
ApaLI G'TGCA_C
Cuts at: 3020 3857 5013 3020
Size: 837 1156 3807
Fragments arranged by size:
3807 1156 837
ApoI r'AATT_y
Cuts at: 1493 3109 3335 3487 5596 1493
Size: 1616 226 152 2109 1697
Fragments arranged by size:
2109 1697 1616 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4354 160
Size: 872 3322 1606
Fragments arranged by size:
3322 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5800
AvaI C'yCGr_G
Cuts at: 1870 1943 2088 2194 2200 2292 2345 2583 3125
Size: 73 145 106 6 92 53 238 542
Cuts at: 3125 1870
Size: 4545
Fragments arranged by size:
4545 542 238 145 106 92 73 53
6
AvrII C'CTAG_G
Cuts at: 2072 2072
Size: 5800
BamHI G'GATC_C
Cuts at: 3130 3497 3130
Size: 367 5433
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2056 2137 3121 4576 618
Size: 325 217 627 269 81 984 1455 1842
Fragments arranged by size:
1842 1455 984 627 325 269 217 81
BanII G_rGCy'C
Cuts at: 1391 2081 2201 2236 2345 2352 2613 2792 2885
Size: 690 120 35 109 7 261 179 93
Cuts at: 2885 2954 3075 3119 1391
Size: 69 121 44 4072
Fragments arranged by size:
4072 690 261 179 121 120 109 93
69 44 35 7
BbeI G_GCGC'C
Cuts at: 2141 2141
Size: 5800
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2454 2625 928
Size: 971 555 171 4103
Fragments arranged by size:
4103 971 555 171
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2283 2317 2336 2370 3991 4025 1903
Size: 34 346 34 19 34 1621 34 3678
Fragments arranged by size:
3678 1621 346 34 34 34 34 19
BciVI GTATCCnnnnn_n'
Cuts at: 2619 3692 4902 2619
Size: 1073 1210 3517
Fragments arranged by size:
3517 1210 1073
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5800
BfrBI ATG'CAT
Cuts at: 2818 2818
Size: 5800
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2091 2363 2787
Size: 107 122 71 631 605 419 272 424
Cuts at: 2787 4411 136
Size: 1624 1525
Fragments arranged by size:
1624 1525 631 605 424 419 272 122
107 71
BglII A'GATC_T
Cuts at: 5795 5795
Size: 5800
BlpI GC'TnA_GC
Cuts at: 3217 3217
Size: 5800
Bme1580I G_kGCm'C
Cuts at: 1790 2195 2201 2236 2345 3024 3075 3861 5017
Size: 405 6 35 109 679 51 786 1156
Cuts at: 5017 1790
Size: 2573
Fragments arranged by size:
2573 1156 786 679 405 109 51 35
6
BmgBI CAC'GTC
Cuts at: 1318 2320 2424 1318
Size: 1002 104 4694
Fragments arranged by size:
4694 1002 104
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3531 4489 442
Size: 416 53 83 399 24 2114 958 1753
Fragments arranged by size:
2114 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5800
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2200 2974 3086 4460 1239
Size: 961 774 112 1374 2579
Fragments arranged by size:
2579 1374 961 774 112
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3024 3925 4790 5088 3024
Size: 901 865 298 3736
Fragments arranged by size:
3736 901 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3002 4463 882
Size: 617 1503 1461 2219
Fragments arranged by size:
2219 1503 1461 617
BsaAI yAC'GTr
Cuts at: 493 1204 3591 493
Size: 711 2387 2702
Fragments arranged by size:
2702 2387 711
BsaBI GATnn'nnATC
Cuts at: 5794 5794
Size: 5800
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2138 3607 3989 4624
Size: 53 83 186 936 605 1469 382 635
Cuts at: 4624 275
Size: 1451
Fragments arranged by size:
1469 1451 936 635 605 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2622 4233 4631 4905 5052 5463 2622
Size: 1611 398 274 147 411 2959
Fragments arranged by size:
2959 1611 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2238 2268 2402 2432 3084
Size: 30 165 30 835 30 134 30 652
Cuts at: 3084 3114 1178
Size: 30 3864
Fragments arranged by size:
3864 835 652 165 134 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2246 1186
Size: 1060 4740
BseYI C'CCAG_C
Cuts at: 2113 2242 2369 2679 2785 3075 5003 5499 2113
Size: 129 127 310 106 290 1928 496 2414
Fragments arranged by size:
2414 1928 496 310 290 129 127 106
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5227
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2014 2289 2395 3250 4011 5039
Size: 275 186 13 275 106 855 761 1028
Cuts at: 5039 1540
Size: 2301
Fragments arranged by size:
2301 1028 855 761 275 275 186 106
13
BsiHKAI G_wGCw'C
Cuts at: 2299 2613 2763 3024 3040 3861 3946 5017 2299
Size: 314 150 261 16 821 85 1071 3082
Fragments arranged by size:
3082 1071 821 314 261 150 85 16
BsmI GAATG_Cn'
Cuts at: 2549 3311 3404 2549
Size: 762 93 4945
Fragments arranged by size:
4945 762 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2422 2422
Size: 5800
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2081 2195 2201 2236 2299 2345 2352
Size: 399 291 114 6 35 63 46 7
Cuts at: 2352 2613 2763 2792 2885 2954 3024 3040 3075
Size: 261 150 29 93 69 70 16 35
Cuts at: 3075 3119 3861 3946 5017 1391
Size: 44 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 291 261 150 114
93 85 70 69 63 46 44 35
35 29 16 7 6
BspEI T'CCGG_A
Cuts at: 5463 5463
Size: 5800
BspHI T'CATG_A
Cuts at: 3689 5419 3689
Size: 1730 4070
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2118 3155 844
Size: 1274 1037 3489
Fragments arranged by size:
3489 1274 1037
BsrBI CCG'CTC
Cuts at: 1394 1823 2291 3687 1394
Size: 429 468 1396 3507
Fragments arranged by size:
3507 1396 468 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4295 4469 65
Size: 1183 3047 174 1396
Fragments arranged by size:
3047 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2009 2134 2281 4444 4631 1700
Size: 110 199 125 147 2163 187 2869
Fragments arranged by size:
2869 2163 199 187 147 125 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5800
BssHII G'CGCG_C
Cuts at: 1925 2796 1925
Size: 871 4929
BssSI C'ACGA_G
Cuts at: 3860 4872 3860
Size: 1012 4788
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5800
BstBI TT'CG_AA
Cuts at: 4604 4604
Size: 5800
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5800
BstXI CCAn_nnnn'nTGG
Cuts at: 3064 3064
Size: 5800
BstYI r'GATC_y
Cuts at: 1972 2159 3130 3497 3882 3899 5351 5437 5449
Size: 187 971 367 385 17 1452 86 12
Cuts at: 5449 5795 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 971 385 367 346 187 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2724 2829 3057 513
Size: 552 1659 105 228 3256
Fragments arranged by size:
3256 1659 552 228 105
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5061
Fragments arranged by size:
5061 683 56
BtsI GCAGTG_nn'
Cuts at: 2517 2734 3316 4110 4130 2517
Size: 217 582 794 20 4187
Fragments arranged by size:
4187 794 582 217 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5800
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5765
DraI TTT'AAA
Cuts at: 3104 3104
Size: 5800
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4807 809
Size: 507 3491 1802
Fragments arranged by size:
3491 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2011 2286 2392
Size: 54 1425 50 275 18 181 275 106
Cuts at: 2392 2700 3247 4136 5765 8
Size: 308 547 889 1629 43
Fragments arranged by size:
1629 1425 889 547 308 275 275 181
106 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2011 2286 2392 3247 1537
Size: 275 199 275 106 855 4090
Fragments arranged by size:
4090 855 275 275 199 106
EarI CTCTTCn'nnn_
Cuts at: 1353 2529 3730 1353
Size: 1176 1201 3423
Fragments arranged by size:
3423 1201 1176
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2367 2758 4387 4761 4907 387
Size: 1064 138 778 391 1629 374 146 1280
Fragments arranged by size:
1629 1280 1064 778 391 374 146 138
EcoICRI GAG'CTC
Cuts at: 2611 2611
Size: 5800
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2200 2466 2974 3086 3863 4460 5247
Size: 393 568 266 508 112 777 597 787
Cuts at: 5247 5515 1239
Size: 268 1524
Fragments arranged by size:
1524 787 777 597 568 508 393 268
266 112
EcoO109I rG'GnC_Cy
Cuts at: 2232 2233 2341 2342 3071 3072 2232
Size: 1 108 1 729 1 4960
Fragments arranged by size:
4960 729 108 1 1 1
EcoRI G'AATT_C
Cuts at: 3109 3487 3109
Size: 378 5422
FspI TGC'GCA
Cuts at: 4306 4306
Size: 5800
HaeII r_GCGC'y
Cuts at: 1678 2054 2141 2680 4947 1678
Size: 376 87 539 2267 2531
Fragments arranged by size:
2531 2267 539 376 87
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2238 2270 2402 2426 2434
Size: 32 135 32 666 32 132 24 8
Cuts at: 2434 2458 2909 2941 4441 4473 4515 4547 1373
Size: 24 451 32 1500 32 42 32 2626
Fragments arranged by size:
2626 1500 666 451 135 132 42 32
32 32 32 32 32 24 24 8
HincII GTy'rAC
Cuts at: 669 1657 1938 3144 3390 669
Size: 988 281 1206 246 3079
Fragments arranged by size:
3079 1206 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5800
HpaI GTT'AAC
Cuts at: 3390 3390
Size: 5800
KasI G'GCGC_C
Cuts at: 2137 2137
Size: 5800
KpnI G_GTAC'C
Cuts at: 3125 3125
Size: 5800
MfeI C'AATT_G
Cuts at: 3399 3399
Size: 5800
MluI A'CGCG_T
Cuts at: 5676 5676
Size: 5800
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2052 2520 3075 4914 5098 1305
Size: 747 468 555 1839 184 2007
Fragments arranged by size:
2007 1839 747 555 468 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3978
Fragments arranged by size:
3978 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2834 3758 4117 4276 518
Size: 648 1668 924 359 159 2042
Fragments arranged by size:
2042 1668 924 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2144 2242 2354 2362 2530 2746 3216 3893
Size: 538 98 112 8 168 216 470 677
Cuts at: 3893 5041 5286 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 538 470 245 216 168
112 98 8
NaeI GCC'GGC
Cuts at: 2136 2283 2136
Size: 147 5653
NarI GG'CG_CC
Cuts at: 2138 2138
Size: 5800
NcoI C'CATG_G
Cuts at: 513 1065 2724 2829 513
Size: 552 1659 105 3484
Fragments arranged by size:
3484 1659 552 105
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5800
NgoMIV G'CCGG_C
Cuts at: 2134 2281 2134
Size: 147 5653
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5800
NotI GC'GGCC_GC
Cuts at: 2286 3247 2286
Size: 961 4839
NsiI A_TGCA'T
Cuts at: 2820 2820
Size: 5800
NspI r_CATG'y
Cuts at: 1132 1229 3158 4703 1132
Size: 97 1929 1545 2229
Fragments arranged by size:
2229 1929 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2337 3026 1348
Size: 989 689 4122
Fragments arranged by size:
4122 989 689
PciI A'CATG_T
Cuts at: 4699 4699
Size: 5800
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5800
PmeI GTTT'AAAC
Cuts at: 3104 3104
Size: 5800
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3869 3901 5382 5414 3869
Size: 32 1481 32 4255
Fragments arranged by size:
4255 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5800
PsiI TTA'TAA
Cuts at: 3370 3370
Size: 5800
PspOMI G'GGCC_C
Cuts at: 2197 2232 2341 3071 2197
Size: 35 109 730 4926
Fragments arranged by size:
4926 730 109 35
PspXI vC'TCGA_Gb
Cuts at: 2292 2292
Size: 5800
PstI C_TGCA'G
Cuts at: 830 3152 830
Size: 2322 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5800
PvuII CAG'CTG
Cuts at: 2530 2530
Size: 5800
SacI G_AGCT'C
Cuts at: 2613 2613
Size: 5800
SalI G'TCGA_C
Cuts at: 1936 3142 1936
Size: 1206 4594
SbfI CC_TGCA'GG
Cuts at: 3152 3152
Size: 5800
ScaI AGT'ACT
Cuts at: 4048 5760 4048
Size: 1712 4088
SfcI C'TryA_G
Cuts at: 826 1045 3148 4283 4964 5155 826
Size: 219 2103 1135 681 191 1471
Fragments arranged by size:
2103 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2139 2139
Size: 5800
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5800
SgrAI Cr'CCGG_yG
Cuts at: 1700 2281 1700
Size: 581 5219
SmaI CCC'GGG
Cuts at: 1872 2090 2196 2202 3127 1872
Size: 218 106 6 925 4545
Fragments arranged by size:
4545 925 218 106 6
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2292 3039 3904 4805 5067 820
Size: 197 926 349 747 865 901 262 1553
Fragments arranged by size:
1553 926 901 865 747 349 262 197
SnaBI TAC'GTA
Cuts at: 493 3591 493
Size: 3098 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5800
SphI G_CATG'C
Cuts at: 1132 3158 1132
Size: 2026 3774
SrfI GCCC'GGGC
Cuts at: 2196 2196
Size: 5800
SspI AAT'ATT
Cuts at: 5 52 3724 5
Size: 47 3672 2081
Fragments arranged by size:
3672 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5800
StyI C'CwwG_G
Cuts at: 513 1065 2072 2478 2724 2829 3239 513
Size: 552 1007 406 246 105 410 3074
Fragments arranged by size:
3074 1007 552 410 406 246 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3827 3844 3997 4182 4627 325
Size: 1255 2247 17 153 185 445 1498
Fragments arranged by size:
2247 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4046 5758 96
Size: 274 80 33 51 1443 2069 1712 138
Fragments arranged by size:
2069 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5760 5792 5760
Size: 32 5768
Tth111I GACn'n_nGTC
Cuts at: 1316 2414 1316
Size: 1098 4702
XbaI T'CTAG_A
Cuts at: 3136 3136
Size: 5800
XhoI C'TCGA_G
Cuts at: 1943 2292 1943
Size: 349 5451
XmaI C'CCGG_G
Cuts at: 1870 2088 2194 2200 3125 1870
Size: 218 106 6 925 4545
Fragments arranged by size:
4545 925 218 106 6
XmnI GAAnn'nnTTC
Cuts at: 2549 3929 2549
Size: 1380 4420
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3608 276
Size: 53 83 186 936 2074 2468
Fragments arranged by size:
2468 2074 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI BaeI BbvCI BplI Bpu10I BsiWI BstZ17I
Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NruI
PacI PfoI PmlI PpuMI PsrI RsrII SacII SanDI
SapI SexAI SfiI SwaI XcmI