Restriction map for
pFN21AB7929
(Circular) (Six-base) MAPSORT of: pFN21AB7929.seq Check: 4819 from: 1 to: 5761
pFN21AB7929 5761 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2341 2341
Size: 5761
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2383 3571 278
Size: 53 83 186 936 847 1188 2468
Fragments arranged by size:
2468 1188 936 847 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3104 1937
Size: 1167 4594
Acc65I G'GTAC_C
Cuts at: 3082 3082
Size: 5761
AclI AA'CG_TT
Cuts at: 1563 3888 4261 1563
Size: 2325 373 3063
Fragments arranged by size:
3063 2325 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2523 2838 3824 5208 5476 1632
Size: 891 315 986 1384 268 1917
Fragments arranged by size:
1917 1384 986 891 315 268
AflII C'TTAA_G
Cuts at: 820 1017 2387 820
Size: 197 1370 4194
Fragments arranged by size:
4194 1370 197
AflIII A'CryG_T
Cuts at: 1203 2701 2910 4660 5637 1203
Size: 1498 209 1750 977 1327
Fragments arranged by size:
1750 1498 1327 977 209
AgeI A'CCGG_T
Cuts at: 4592 4592
Size: 5761
AhdI GACnn_n'nnGTC
Cuts at: 4490 4490
Size: 5761
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5761
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5761
AlwNI CAG_nnn'CTG
Cuts at: 2236 2816 3504 5076 2236
Size: 580 688 1572 2921
Fragments arranged by size:
2921 1572 688 580
ApaLI G'TGCA_C
Cuts at: 3818 4974 3818
Size: 1156 4605
ApoI r'AATT_y
Cuts at: 1493 2587 2777 3070 3296 3448 5557 1493
Size: 1094 190 293 226 152 2109 1697
Fragments arranged by size:
2109 1697 1094 293 226 190 152
AseI AT'TA_AT
Cuts at: 160 1032 4315 160
Size: 872 3283 1606
Fragments arranged by size:
3283 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5761
AvaI C'yCGr_G
Cuts at: 1870 1943 2126 2755 3086 1870
Size: 73 183 629 331 4545
Fragments arranged by size:
4545 629 331 183 73
BamHI G'GATC_C
Cuts at: 2858 3091 3458 2858
Size: 233 367 5161
Fragments arranged by size:
5161 367 233
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2060 2192 2644 3082 4537
Size: 325 217 627 273 132 452 438 1455
Cuts at: 4537 618
Size: 1842
Fragments arranged by size:
1842 1455 627 452 438 325 273 217
132
BanII G_rGCy'C
Cuts at: 1391 3080 1391
Size: 1689 4072
BbeI G_GCGC'C
Cuts at: 2064 2196 2064
Size: 132 5629
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2011 928
Size: 971 112 4678
Fragments arranged by size:
4678 971 112
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3952 3986 1903
Size: 34 2015 34 3678
Fragments arranged by size:
3678 2015 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3653 4863 3653
Size: 1210 4551
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5761
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4372 136
Size: 107 122 71 631 605 2700 1525
Fragments arranged by size:
2700 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5756 5756
Size: 5761
BlpI GC'TnA_GC
Cuts at: 2030 2474 3178 2030
Size: 444 704 4613
Fragments arranged by size:
4613 704 444
Bme1580I G_kGCm'C
Cuts at: 1790 3822 4978 1790
Size: 2032 1156 2573
Fragments arranged by size:
2573 2032 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5761
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2552 3492 4450
Size: 416 53 83 399 24 1135 940 958
Cuts at: 4450 442
Size: 1753
Fragments arranged by size:
1753 1135 958 940 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5761
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4421 1239
Size: 3182 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2141 2243 3886 4751 5049 2141
Size: 102 1643 865 298 2853
Fragments arranged by size:
2853 1643 865 298 102
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4424 882
Size: 617 2925 2219
Fragments arranged by size:
2925 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2641 2913 3552 493
Size: 711 1437 272 639 2702
Fragments arranged by size:
2702 1437 711 639 272
BsaBI GATnn'nnATC
Cuts at: 5755 5755
Size: 5761
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2061 2193 2380 3568
Size: 53 83 186 936 528 132 187 1188
Cuts at: 3568 3950 4585 275
Size: 382 635 1451
Fragments arranged by size:
1451 1188 936 635 528 382 187 186
132 83 53
BsaWI w'CCGG_w
Cuts at: 2181 4194 4592 4866 5013 5424 2181
Size: 2013 398 274 147 411 2518
Fragments arranged by size:
2518 2013 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5536
Fragments arranged by size:
5536 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5761
BseYI C'CCAG_C
Cuts at: 2418 4964 5460 2418
Size: 2546 496 2719
Fragments arranged by size:
2719 2546 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2369 3071 1163
Size: 573 633 702 3853
Fragments arranged by size:
3853 702 633 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3211 3972 5000 1540
Size: 275 186 1210 761 1028 2301
Fragments arranged by size:
2301 1210 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3822 3907 4978 3822
Size: 85 1071 4605
Fragments arranged by size:
4605 1071 85
BsmI GAATG_Cn'
Cuts at: 2136 3272 3365 2136
Size: 1136 93 4532
Fragments arranged by size:
4532 1136 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3080 3822 3907 4978 1391
Size: 399 1290 742 85 1071 2174
Fragments arranged by size:
2174 1290 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5424 5424
Size: 5761
BspHI T'CATG_A
Cuts at: 3650 5380 3650
Size: 1730 4031
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2341 3116 844
Size: 1497 775 3489
Fragments arranged by size:
3489 1497 775
BsrBI CCG'CTC
Cuts at: 1394 1823 2071 2077 3648 1394
Size: 429 248 6 1571 3507
Fragments arranged by size:
3507 1571 429 248 6
BsrDI GCAATG_nn'
Cuts at: 65 1248 2467 4256 4430 65
Size: 1183 1219 1789 174 1396
Fragments arranged by size:
1789 1396 1219 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2085 2189 4405 4592 1700
Size: 110 275 104 2216 187 2869
Fragments arranged by size:
2869 2216 275 187 110 104
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5761
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5761
BssSI C'ACGA_G
Cuts at: 3821 4833 3821
Size: 1012 4749
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2236 794
Size: 1442 4319
BstBI TT'CG_AA
Cuts at: 4565 4565
Size: 5761
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5761
BstYI r'GATC_y
Cuts at: 1972 2759 2858 3091 3458 3843 3860 5312 5398
Size: 787 99 233 367 385 17 1452 86
Cuts at: 5398 5410 5756 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 787 385 367 346 233 99
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5209
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5022
Fragments arranged by size:
5022 683 56
BtsI GCAGTG_nn'
Cuts at: 2270 2491 3277 4071 4091 2270
Size: 221 786 794 20 3940
Fragments arranged by size:
3940 794 786 221 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5761
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5726
DraI TTT'AAA
Cuts at: 3065 3065
Size: 5761
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4768 809
Size: 507 3452 1802
Fragments arranged by size:
3452 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2083 2709 3208
Size: 54 1425 50 275 18 253 626 499
Cuts at: 3208 4097 5726 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 626 499 275 253 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3208 1537
Size: 275 1396 4090
Fragments arranged by size:
4090 1396 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3691 1353
Size: 2338 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4348 4722 4868 387
Size: 1064 138 2759 374 146 1280
Fragments arranged by size:
2759 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2523 2838 3824 4421 5208 5476 1239
Size: 393 891 315 986 597 787 268 1524
Fragments arranged by size:
1524 986 891 787 597 393 315 268
EcoO109I rG'GnC_Cy
Cuts at: 2233 2233
Size: 5761
EcoRI G'AATT_C
Cuts at: 2587 3070 3448 2587
Size: 483 378 4900
Fragments arranged by size:
4900 483 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2335 2439 2683 2335
Size: 104 244 5413
Fragments arranged by size:
5413 244 104
FseI GG_CCGG'CC
Cuts at: 2089 2089
Size: 5761
FspI TGC'GCA
Cuts at: 4267 4267
Size: 5761
HaeII r_GCGC'y
Cuts at: 1678 2064 2196 4908 1678
Size: 386 132 2712 2531
Fragments arranged by size:
2712 2531 386 132
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4402 4434 4476 4508 1373
Size: 32 135 32 2830 32 42 32 2626
Fragments arranged by size:
2830 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3105 3351 669
Size: 988 281 1167 246 3079
Fragments arranged by size:
3079 1167 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5761
HpaI GTT'AAC
Cuts at: 3351 3351
Size: 5761
KasI G'GCGC_C
Cuts at: 2060 2192 2060
Size: 132 5629
KpnI G_GTAC'C
Cuts at: 3086 3086
Size: 5761
MfeI C'AATT_G
Cuts at: 3360 3360
Size: 5761
MluI A'CGCG_T
Cuts at: 5637 5637
Size: 5761
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4875 5059 1305
Size: 3570 184 2007
Fragments arranged by size:
3570 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2711 10
Size: 54 1425 343 879 3060
Fragments arranged by size:
3060 1425 879 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2723 3719 4078 4237 518
Size: 648 1557 996 359 159 2042
Fragments arranged by size:
2042 1557 996 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2034 2097 2991 3046 3177 3854 5002 5247
Size: 428 63 894 55 131 677 1148 245
Cuts at: 5247 1606
Size: 2120
Fragments arranged by size:
2120 1148 894 677 428 245 131 63
55
NaeI GCC'GGC
Cuts at: 2087 2087
Size: 5761
NarI GG'CG_CC
Cuts at: 2061 2193 2061
Size: 132 5629
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5209
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5761
NgoMIV G'CCGG_C
Cuts at: 2085 2085
Size: 5761
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5761
NotI GC'GGCC_GC
Cuts at: 3208 3208
Size: 5761
NspI r_CATG'y
Cuts at: 1132 1229 2705 3119 4664 1132
Size: 97 1476 414 1545 2229
Fragments arranged by size:
2229 1545 1476 414 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5761
PciI A'CATG_T
Cuts at: 2701 4660 2701
Size: 1959 3802
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5761
PfoI T'CCnGG_A
Cuts at: 2103 2103
Size: 5761
PmeI GTTT'AAAC
Cuts at: 3065 3065
Size: 5761
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3830 3862 5343 5375 3830
Size: 32 1481 32 4216
Fragments arranged by size:
4216 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5761
PsiI TTA'TAA
Cuts at: 3331 3331
Size: 5761
PstI C_TGCA'G
Cuts at: 830 2979 3113 830
Size: 2149 134 3478
Fragments arranged by size:
3478 2149 134
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5761
PvuII CAG'CTG
Cuts at: 2991 2991
Size: 5761
SalI G'TCGA_C
Cuts at: 1936 3103 1936
Size: 1167 4594
SbfI CC_TGCA'GG
Cuts at: 3113 3113
Size: 5761
ScaI AGT'ACT
Cuts at: 4009 5721 4009
Size: 1712 4049
SfcI C'TryA_G
Cuts at: 826 1045 2092 2975 3109 4244 4925 5116 826
Size: 219 1047 883 134 1135 681 191 1471
Fragments arranged by size:
1471 1135 1047 883 681 219 191 134
SfoI GGC'GCC
Cuts at: 2062 2194 2062
Size: 132 5629
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5761
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5761
SmaI CCC'GGG
Cuts at: 1872 3088 1872
Size: 1216 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2156 2258 2387 3865 4766 5028
Size: 197 926 213 102 129 1478 901 262
Cuts at: 5028 820
Size: 1553
Fragments arranged by size:
1553 1478 926 901 262 213 197 129
102
SnaBI TAC'GTA
Cuts at: 493 3552 493
Size: 3059 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5761
SphI G_CATG'C
Cuts at: 1132 3119 1132
Size: 1987 3774
SspI AAT'ATT
Cuts at: 5 52 3685 5
Size: 47 3633 2081
Fragments arranged by size:
3633 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5761
StyI C'CwwG_G
Cuts at: 513 1065 3200 513
Size: 552 2135 3074
Fragments arranged by size:
3074 2135 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2321 2581 3788 3805 3958 4143 4588
Size: 1255 741 260 1207 17 153 185 445
Cuts at: 4588 325
Size: 1498
Fragments arranged by size:
1498 1255 1207 741 445 260 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4007 5719 96
Size: 274 80 33 51 1443 2030 1712 138
Fragments arranged by size:
2030 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5721 5753 5721
Size: 32 5729
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5761
XbaI T'CTAG_A
Cuts at: 3097 3097
Size: 5761
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2415 2415
Size: 5761
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5761
XmaI C'CCGG_G
Cuts at: 1870 3086 1870
Size: 1216 4545
XmnI GAAnn'nnTTC
Cuts at: 3890 3890
Size: 5761
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2381 3569 276
Size: 53 83 186 936 847 1188 2468
Fragments arranged by size:
2468 1188 936 847 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FspAI NruI NsiI PacI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SrfI SwaI