Restriction map for
pFN21AB7918
(Circular) (Six-base) MAPSORT of: pFN21AB7918.seq Check: 7160 from: 1 to: 5743
pFN21AB7918 5743 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3553 278
Size: 53 83 186 936 2017 2468
Fragments arranged by size:
2468 2017 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3086 1937
Size: 1149 4594
Acc65I G'GTAC_C
Cuts at: 3064 3064
Size: 5743
AclI AA'CG_TT
Cuts at: 1563 3870 4243 1563
Size: 2307 373 3063
Fragments arranged by size:
3063 2307 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2437 2606 2987 3806 5190 5458 1632
Size: 805 169 381 819 1384 268 1917
Fragments arranged by size:
1917 1384 819 805 381 268 169
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5546
AflIII A'CryG_T
Cuts at: 1203 4642 5619 1203
Size: 3439 977 1327
Fragments arranged by size:
3439 1327 977
AgeI A'CCGG_T
Cuts at: 4574 4574
Size: 5743
AhdI GACnn_n'nnGTC
Cuts at: 2262 4472 2262
Size: 2210 3533
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5743
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5743
AlwNI CAG_nnn'CTG
Cuts at: 2414 3486 5058 2414
Size: 1072 1572 3099
Fragments arranged by size:
3099 1572 1072
ApaI G_GGCC'C
Cuts at: 2387 2387
Size: 5743
ApaLI G'TGCA_C
Cuts at: 3800 4956 3800
Size: 1156 4587
ApoI r'AATT_y
Cuts at: 1493 3052 3278 3430 5539 1493
Size: 1559 226 152 2109 1697
Fragments arranged by size:
2109 1697 1559 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4297 160
Size: 872 3265 1606
Fragments arranged by size:
3265 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5743
AvaI C'yCGr_G
Cuts at: 1870 1943 2020 2081 2380 2697 3068 1870
Size: 73 77 61 299 317 371 4545
Fragments arranged by size:
4545 371 317 299 77 73 61
BamHI G'GATC_C
Cuts at: 2029 2838 3073 3440 2029
Size: 809 235 367 4332
Fragments arranged by size:
4332 809 367 235
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2294 3032 3064 4519 618
Size: 325 217 627 507 738 32 1455 1842
Fragments arranged by size:
1842 1455 738 627 507 325 217 32
BanII G_rGCy'C
Cuts at: 1391 2387 2719 2943 2995 3062 1391
Size: 996 332 224 52 67 4072
Fragments arranged by size:
4072 996 332 224 67 52
BbeI G_GCGC'C
Cuts at: 2298 2298
Size: 5743
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4772
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3934 3968 1903
Size: 34 1997 34 3678
Fragments arranged by size:
3678 1997 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3635 4845 3635
Size: 1210 4533
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5743
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4354 136
Size: 107 122 71 631 605 2682 1525
Fragments arranged by size:
2682 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5738 5738
Size: 5743
BlpI GC'TnA_GC
Cuts at: 3160 3160
Size: 5743
Bme1580I G_kGCm'C
Cuts at: 1790 2387 2406 3035 3804 4960 1790
Size: 597 19 629 769 1156 2573
Fragments arranged by size:
2573 1156 769 629 597 19
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5743
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3474 4432 442
Size: 416 53 83 399 24 2057 958 1753
Fragments arranged by size:
2057 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5743
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2601 3035 4403 1239
Size: 1362 434 1368 2579
Fragments arranged by size:
2579 1368 1362 434
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3868 4733 5031 3868
Size: 865 298 4580
Fragments arranged by size:
4580 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2894 4406 882
Size: 617 1395 1512 2219
Fragments arranged by size:
2219 1512 1395 617
BsaAI yAC'GTr
Cuts at: 493 1204 3534 493
Size: 711 2330 2702
Fragments arranged by size:
2702 2330 711
BsaBI GATnn'nnATC
Cuts at: 5737 5737
Size: 5743
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2097 2295 2606 3550
Size: 53 83 186 936 564 198 311 944
Cuts at: 3550 3932 4567 275
Size: 382 635 1451
Fragments arranged by size:
1451 944 936 635 564 382 311 198
186 83 53
BsaWI w'CCGG_w
Cuts at: 4176 4574 4848 4995 5406 4176
Size: 398 274 147 411 4513
Fragments arranged by size:
4513 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2217 2247 1178
Size: 30 165 30 814 30 4674
Fragments arranged by size:
4674 814 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2105 2150 2722 2975 1186
Size: 919 45 572 253 3954
Fragments arranged by size:
3954 919 572 253 45
BseYI C'CCAG_C
Cuts at: 2373 2515 2718 2994 4946 5442 2373
Size: 142 203 276 1952 496 2674
Fragments arranged by size:
2674 1952 496 276 203 142
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5170
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2478 2646 3193 3954 4982 1540
Size: 275 186 477 168 547 761 1028 2301
Fragments arranged by size:
2301 1028 761 547 477 275 186 168
BsiHKAI G_wGCw'C
Cuts at: 2943 3804 3889 4960 2943
Size: 861 85 1071 3726
Fragments arranged by size:
3726 1071 861 85
BsmI GAATG_Cn'
Cuts at: 3254 3347 3254
Size: 93 5650
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2387 2406 2719 2943 2995 3035 3062
Size: 399 597 19 313 224 52 40 27
Cuts at: 3062 3804 3889 4960 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 597 399 313 224 85
52 40 27 19
BspEI T'CCGG_A
Cuts at: 5406 5406
Size: 5743
BspHI T'CATG_A
Cuts at: 2598 3632 5362 2598
Size: 1034 1730 2979
Fragments arranged by size:
2979 1730 1034
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3031 3098 844
Size: 2187 67 3489
Fragments arranged by size:
3489 2187 67
BsrBI CCG'CTC
Cuts at: 1394 1823 2132 2737 2920 3630 1394
Size: 429 309 605 183 710 3507
Fragments arranged by size:
3507 710 605 429 309 183
BsrDI GCAATG_nn'
Cuts at: 65 1248 2464 4238 4412 65
Size: 1183 1216 1774 174 1396
Fragments arranged by size:
1774 1396 1216 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4387 4574 1700
Size: 110 2577 187 2869
Fragments arranged by size:
2869 2577 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5743
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5743
BssSI C'ACGA_G
Cuts at: 3803 4815 3803
Size: 1012 4731
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2504 794
Size: 1710 4033
BstBI TT'CG_AA
Cuts at: 2780 4547 2780
Size: 1767 3976
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5743
BstYI r'GATC_y
Cuts at: 1972 2029 2838 3073 3440 3825 3842 5294 5380
Size: 57 809 235 367 385 17 1452 86
Cuts at: 5380 5392 5738 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 809 385 367 346 235 86
57 17 12
Bsu36I CC'TnA_GG
Cuts at: 2945 2945
Size: 5743
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4252
Fragments arranged by size:
4252 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5004
Fragments arranged by size:
5004 683 56
BtsI GCAGTG_nn'
Cuts at: 3259 4053 4073 3259
Size: 794 20 4929
Fragments arranged by size:
4929 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5743
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5708
DraI TTT'AAA
Cuts at: 3047 3047
Size: 5743
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4750 809
Size: 507 3434 1802
Fragments arranged by size:
3434 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2043 2151 2275
Size: 54 1425 50 275 18 213 108 124
Cuts at: 2275 2475 3190 4079 5708 8
Size: 200 715 889 1629 43
Fragments arranged by size:
1629 1425 889 715 275 213 200 124
108 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2475 3190 1537
Size: 275 663 715 4090
Fragments arranged by size:
4090 715 663 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2707 3673 1353
Size: 1354 966 3423
Fragments arranged by size:
3423 1354 966
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2102 2758 4330 4704 4850 387
Size: 1064 138 513 656 1572 374 146 1280
Fragments arranged by size:
1572 1280 1064 656 513 374 146 138
EcoICRI GAG'CTC
Cuts at: 2941 2941
Size: 5743
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2437 2601 2606 2987 3035 3806 4403
Size: 393 805 164 5 381 48 771 597
Cuts at: 4403 5190 5458 1239
Size: 787 268 1524
Fragments arranged by size:
1524 805 787 771 597 393 381 268
164 48 5
EcoNI CCTnn'n_nnAGG
Cuts at: 2846 2846
Size: 5743
EcoO109I rG'GnC_Cy
Cuts at: 2078 2384 2411 2078
Size: 306 27 5410
Fragments arranged by size:
5410 306 27
EcoRI G'AATT_C
Cuts at: 3052 3430 3052
Size: 378 5365
FspI TGC'GCA
Cuts at: 4249 4249
Size: 5743
HaeII r_GCGC'y
Cuts at: 1678 2298 2380 2423 4890 1678
Size: 620 82 43 2467 2531
Fragments arranged by size:
2531 2467 620 82 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2248 2280 2431 2463 4384
Size: 32 135 32 676 32 151 32 1921
Cuts at: 4384 4416 4458 4490 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1921 676 151 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3087 3333 669
Size: 988 281 1149 246 3079
Fragments arranged by size:
3079 1149 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5743
HpaI GTT'AAC
Cuts at: 3333 3333
Size: 5743
KasI G'GCGC_C
Cuts at: 2294 2294
Size: 5743
KpnI G_GTAC'C
Cuts at: 3068 3068
Size: 5743
MfeI C'AATT_G
Cuts at: 3342 3342
Size: 5743
MluI A'CGCG_T
Cuts at: 5619 5619
Size: 5743
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4857 5041 1305
Size: 3552 184 2007
Fragments arranged by size:
3552 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2153 10
Size: 54 1425 343 321 3600
Fragments arranged by size:
3600 1425 343 321 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3701 4060 4219 518
Size: 648 843 1692 359 159 2042
Fragments arranged by size:
2042 1692 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2036 2051 2147 2269 2291 2318 2393 2431
Size: 430 15 96 122 22 27 75 38
Cuts at: 2431 2490 2998 3159 3836 4984 5229 1606
Size: 59 508 161 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 508 430 245 161 122
96 75 59 38 27 22 15
NarI GG'CG_CC
Cuts at: 2295 2295
Size: 5743
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4252
Fragments arranged by size:
4252 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5743
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5743
NotI GC'GGCC_GC
Cuts at: 3190 3190
Size: 5743
NspI r_CATG'y
Cuts at: 1132 1229 2014 2896 3101 4646 1132
Size: 97 785 882 205 1545 2229
Fragments arranged by size:
2229 1545 882 785 205 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5743
PciI A'CATG_T
Cuts at: 4642 4642
Size: 5743
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5743
PfoI T'CCnGG_A
Cuts at: 2865 2865
Size: 5743
PmeI GTTT'AAAC
Cuts at: 3047 3047
Size: 5743
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3812 3844 5325 5357 3812
Size: 32 1481 32 4198
Fragments arranged by size:
4198 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2078 2411 2078
Size: 333 5410
PshAI GACnn'nnGTC
Cuts at: 1613 2022 1613
Size: 409 5334
PsiI TTA'TAA
Cuts at: 3313 3313
Size: 5743
PspOMI G'GGCC_C
Cuts at: 2383 2383
Size: 5743
PstI C_TGCA'G
Cuts at: 830 2109 2419 2928 3003 3095 830
Size: 1279 310 509 75 92 3478
Fragments arranged by size:
3478 1279 509 310 92 75
PvuI CG_AT'CG
Cuts at: 2001 2646 2001
Size: 645 5098
PvuII CAG'CTG
Cuts at: 2269 2291 2998 2269
Size: 22 707 5014
Fragments arranged by size:
5014 707 22
SacI G_AGCT'C
Cuts at: 2943 2943
Size: 5743
SalI G'TCGA_C
Cuts at: 1936 3085 1936
Size: 1149 4594
SanDI GG'GwC_CC
Cuts at: 2078 2078
Size: 5743
SapI GCTCTTCn'nnn_
Cuts at: 2707 2707
Size: 5743
SbfI CC_TGCA'GG
Cuts at: 3095 3095
Size: 5743
ScaI AGT'ACT
Cuts at: 3991 5703 3991
Size: 1712 4031
SfcI C'TryA_G
Cuts at: 826 1045 2105 2415 2924 2999 3091 4226 4907
Size: 219 1060 310 509 75 92 1135 681
Cuts at: 4907 5098 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1060 681 509 310 219 191
92 75
SfoI GGC'GCC
Cuts at: 2296 2296
Size: 5743
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5743
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5743
SmaI CCC'GGG
Cuts at: 1872 2022 2382 3070 1872
Size: 150 360 688 4545
Fragments arranged by size:
4545 688 360 150
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3847 4748 5010 820
Size: 197 926 1904 901 262 1553
Fragments arranged by size:
1904 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3534 493
Size: 3041 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5743
SphI G_CATG'C
Cuts at: 1132 2896 3101 1132
Size: 1764 205 3774
Fragments arranged by size:
3774 1764 205
SrfI GCCC'GGGC
Cuts at: 2382 2382
Size: 5743
SspI AAT'ATT
Cuts at: 5 52 3667 5
Size: 47 3615 2081
Fragments arranged by size:
3615 2081 47
StuI AGG'CCT
Cuts at: 1517 2852 1517
Size: 1335 4408
StyI C'CwwG_G
Cuts at: 513 1065 2004 2847 3182 513
Size: 552 939 843 335 3074
Fragments arranged by size:
3074 939 843 552 335
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2345 3770 3787 3940 4125 4570 325
Size: 1255 765 1425 17 153 185 445 1498
Fragments arranged by size:
1498 1425 1255 765 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3989 5701 96
Size: 274 80 33 51 1443 2012 1712 138
Fragments arranged by size:
2012 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2217 2249 5703 5735 2217
Size: 32 3454 32 2225
Fragments arranged by size:
3454 2225 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5743
XbaI T'CTAG_A
Cuts at: 3079 3079
Size: 5743
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2258 2011
Size: 247 5496
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5743
XmaI C'CCGG_G
Cuts at: 1870 2020 2380 3068 1870
Size: 150 360 688 4545
Fragments arranged by size:
4545 688 360 150
XmnI GAAnn'nnTTC
Cuts at: 3872 3872
Size: 5743
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3551 276
Size: 53 83 186 936 2017 2468
Fragments arranged by size:
2468 2017 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI RsrII SacII SexAI SfiI SwaI