Restriction map for
pFN21AB7912
(Circular) (Six-base) MAPSORT of: pFN21AB7912.seq Check: 1474 from: 1 to: 5698
pFN21AB7912 5698 bp
With 182 enzymes: SgfI *
March 28, 2012 12:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2802 2802
Size: 5698
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3508 278
Size: 53 83 186 936 1972 2468
Fragments arranged by size:
2468 1972 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3041 1937
Size: 1104 4594
Acc65I G'GTAC_C
Cuts at: 3019 3019
Size: 5698
AclI AA'CG_TT
Cuts at: 1563 3825 4198 1563
Size: 2262 373 3063
Fragments arranged by size:
3063 2262 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3761 5145 5413 1632
Size: 2129 1384 268 1917
Fragments arranged by size:
2129 1917 1384 268
AfeI AGC'GCT
Cuts at: 2770 2770
Size: 5698
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5501
AflIII A'CryG_T
Cuts at: 1203 4597 5574 1203
Size: 3394 977 1327
Fragments arranged by size:
3394 1327 977
AgeI A'CCGG_T
Cuts at: 4529 4529
Size: 5698
AhdI GACnn_n'nnGTC
Cuts at: 4427 4427
Size: 5698
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5698
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5698
AlwNI CAG_nnn'CTG
Cuts at: 2533 3441 5013 2533
Size: 908 1572 3218
Fragments arranged by size:
3218 1572 908
ApaLI G'TGCA_C
Cuts at: 3755 4911 3755
Size: 1156 4542
ApoI r'AATT_y
Cuts at: 1493 3007 3233 3385 5494 1493
Size: 1514 226 152 2109 1697
Fragments arranged by size:
2109 1697 1514 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4252 160
Size: 872 3220 1606
Fragments arranged by size:
3220 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5698
AvaI C'yCGr_G
Cuts at: 1870 1943 2550 3023 1870
Size: 73 607 473 4545
Fragments arranged by size:
4545 607 473 73
BamHI G'GATC_C
Cuts at: 3028 3395 3028
Size: 367 5331
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2303 3019 4474 618
Size: 325 217 627 516 716 1455 1842
Fragments arranged by size:
1842 1455 716 627 516 325 217
BanII G_rGCy'C
Cuts at: 1391 3017 1391
Size: 1626 4072
BbeI G_GCGC'C
Cuts at: 2307 2307
Size: 5698
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4727
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3889 3923 1903
Size: 34 1952 34 3678
Fragments arranged by size:
3678 1952 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3590 4800 3590
Size: 1210 4488
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5698
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2870 4309 136
Size: 107 122 71 631 605 1198 1439 1525
Fragments arranged by size:
1525 1439 1198 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5693 5693
Size: 5698
BlpI GC'TnA_GC
Cuts at: 3115 3115
Size: 5698
Bme1580I G_kGCm'C
Cuts at: 1790 3759 4915 1790
Size: 1969 1156 2573
Fragments arranged by size:
2573 1969 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5698
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2543 3429 4387
Size: 416 53 83 399 24 1126 886 958
Cuts at: 4387 442
Size: 1753
Fragments arranged by size:
1753 1126 958 886 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5698
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2111 2286 2489 2926 4358 1239
Size: 872 175 203 437 1432 2579
Fragments arranged by size:
2579 1432 872 437 203 175
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3823 4688 4986 3823
Size: 865 298 4535
Fragments arranged by size:
4535 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2480 4361 882
Size: 617 981 1881 2219
Fragments arranged by size:
2219 1881 981 617
BsaAI yAC'GTr
Cuts at: 493 1204 2076 3489 493
Size: 711 872 1413 2702
Fragments arranged by size:
2702 1413 872 711
BsaBI GATnn'nnATC
Cuts at: 5692 5692
Size: 5698
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2304 2502 2726 3505
Size: 53 83 186 936 771 198 224 779
Cuts at: 3505 3887 4522 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 779 771 635 382 224 198
186 83 53
BsaWI w'CCGG_w
Cuts at: 4131 4529 4803 4950 5361 4131
Size: 398 274 147 411 4468
Fragments arranged by size:
4468 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2220 2250 1178
Size: 30 165 30 817 30 4626
Fragments arranged by size:
4626 817 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2214 2235 2244 2256 2259 2262 2274 2283
Size: 1028 21 9 12 3 3 12 9
Cuts at: 2283 1186
Size: 4601
Fragments arranged by size:
4601 1028 21 12 12 9 9 3
3
BseYI C'CCAG_C
Cuts at: 2836 4901 5397 2836
Size: 2065 496 3137
Fragments arranged by size:
3137 2065 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2116 2576 1163
Size: 573 380 460 4285
Fragments arranged by size:
4285 573 460 380
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2127 2155 2181 2910 3148 3909
Size: 275 186 126 28 26 729 238 761
Cuts at: 3909 4937 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 729 275 238 186 126
28 26
BsiHKAI G_wGCw'C
Cuts at: 3759 3844 4915 3759
Size: 85 1071 4542
Fragments arranged by size:
4542 1071 85
BsmI GAATG_Cn'
Cuts at: 3209 3302 3209
Size: 93 5605
BsmBI CGTCTCn'nnnn_
Cuts at: 2032 2032
Size: 5698
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3017 3759 3844 4915 1391
Size: 399 1227 742 85 1071 2174
Fragments arranged by size:
2174 1227 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5361 5361
Size: 5698
BspHI T'CATG_A
Cuts at: 3587 5317 3587
Size: 1730 3968
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2548 2802 3053 844
Size: 1704 254 251 3489
Fragments arranged by size:
3489 1704 254 251
BsrBI CCG'CTC
Cuts at: 1394 1823 2097 2860 3585 1394
Size: 429 274 763 725 3507
Fragments arranged by size:
3507 763 725 429 274
BsrDI GCAATG_nn'
Cuts at: 65 1248 2713 4193 4367 65
Size: 1183 1465 1480 174 1396
Fragments arranged by size:
1480 1465 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2126 2180 4342 4529 1700
Size: 110 316 54 2162 187 2869
Fragments arranged by size:
2869 2162 316 187 110 54
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5698
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5698
BssSI C'ACGA_G
Cuts at: 3758 4770 3758
Size: 1012 4686
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5698
BstBI TT'CG_AA
Cuts at: 4502 4502
Size: 5698
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5698
BstYI r'GATC_y
Cuts at: 1972 3028 3395 3780 3797 5249 5335 5347 5693
Size: 1056 367 385 17 1452 86 12 346
Cuts at: 5693 1972
Size: 1977
Fragments arranged by size:
1977 1452 1056 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2874 513
Size: 552 939 870 3337
Fragments arranged by size:
3337 939 870 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2932 487
Size: 683 56 1706 3253
Fragments arranged by size:
3253 1706 683 56
BtsI GCAGTG_nn'
Cuts at: 3214 4008 4028 3214
Size: 794 20 4884
Fragments arranged by size:
4884 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5698
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5663
DraI TTT'AAA
Cuts at: 3002 3002
Size: 5698
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2350 2731 4705 809
Size: 507 1034 381 1974 1802
Fragments arranged by size:
1974 1802 1034 507 381
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2178 2808 2871
Size: 54 1425 50 275 18 348 630 63
Cuts at: 2871 2907 3145 4034 5663 8
Size: 36 238 889 1629 43
Fragments arranged by size:
1629 1425 889 630 348 275 238 63
54 50 43 36 18
EagI C'GGCC_G
Cuts at: 1537 1812 2178 2907 3145 1537
Size: 275 366 729 238 4090
Fragments arranged by size:
4090 729 366 275 238
EarI CTCTTCn'nnn_
Cuts at: 1353 2572 3628 1353
Size: 1219 1056 3423
Fragments arranged by size:
3423 1219 1056
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2199 2211 2220 2232 2241 2271
Size: 1064 138 610 12 9 12 9 30
Cuts at: 2271 2878 4285 4659 4805 387
Size: 607 1407 374 146 1280
Fragments arranged by size:
1407 1280 1064 610 607 374 146 138
30 12 12 9 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2111 2286 2489 2926 3761 4358 5145
Size: 393 479 175 203 437 835 597 787
Cuts at: 5145 5413 1239
Size: 268 1524
Fragments arranged by size:
1524 835 787 597 479 437 393 268
203 175
EcoNI CCTnn'n_nnAGG
Cuts at: 2543 2543
Size: 5698
EcoRI G'AATT_C
Cuts at: 3007 3385 3007
Size: 378 5320
FspI TGC'GCA
Cuts at: 4204 4204
Size: 5698
HaeII r_GCGC'y
Cuts at: 1678 2278 2307 2772 4845 1678
Size: 600 29 465 2073 2531
Fragments arranged by size:
2531 2073 600 465 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2416 2448 2479 2511 2844
Size: 32 135 32 844 32 31 32 333
Cuts at: 2844 2876 4339 4371 4413 4445 1373
Size: 32 1463 32 42 32 2626
Fragments arranged by size:
2626 1463 844 333 135 42 32 32
32 32 32 32 32 31
HincII GTy'rAC
Cuts at: 669 1657 1938 2674 3042 3288 669
Size: 988 281 736 368 246 3079
Fragments arranged by size:
3079 988 736 368 281 246
HindIII A'AGCT_T
Cuts at: 748 2649 748
Size: 1901 3797
HpaI GTT'AAC
Cuts at: 3288 3288
Size: 5698
KasI G'GCGC_C
Cuts at: 2303 2303
Size: 5698
KpnI G_GTAC'C
Cuts at: 3023 3023
Size: 5698
MfeI C'AATT_G
Cuts at: 3297 3297
Size: 5698
MluI A'CGCG_T
Cuts at: 5574 5574
Size: 5698
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2414 2523 4812 4996 1305
Size: 1109 109 2289 184 2007
Fragments arranged by size:
2289 2007 1109 184 109
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3876
Fragments arranged by size:
3876 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2616 3656 4015 4174 518
Size: 648 1450 1040 359 159 2042
Fragments arranged by size:
2042 1450 1040 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2104 2755 2761 2876 3114 3791 4939 5184
Size: 498 651 6 115 238 677 1148 245
Cuts at: 5184 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 651 498 245 238 115
6
NaeI GCC'GGC
Cuts at: 2182 2182
Size: 5698
NarI GG'CG_CC
Cuts at: 2304 2304
Size: 5698
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4207
Fragments arranged by size:
4207 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5698
NgoMIV G'CCGG_C
Cuts at: 2180 2180
Size: 5698
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5698
NotI GC'GGCC_GC
Cuts at: 2907 3145 2907
Size: 238 5460
NspI r_CATG'y
Cuts at: 1132 1229 3056 4601 1132
Size: 97 1827 1545 2229
Fragments arranged by size:
2229 1827 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2989 1348
Size: 1641 4057
PciI A'CATG_T
Cuts at: 4597 4597
Size: 5698
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2533 1108
Size: 1425 4273
PfoI T'CCnGG_A
Cuts at: 2286 2286
Size: 5698
PmeI GTTT'AAAC
Cuts at: 3002 3002
Size: 5698
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3767 3799 5280 5312 3767
Size: 32 1481 32 4153
Fragments arranged by size:
4153 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5698
PsiI TTA'TAA
Cuts at: 3268 3268
Size: 5698
PstI C_TGCA'G
Cuts at: 830 2760 3050 830
Size: 1930 290 3478
Fragments arranged by size:
3478 1930 290
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5698
PvuII CAG'CTG
Cuts at: 2755 2761 2755
Size: 6 5692
SacII CC_GC'GG
Cuts at: 2877 2877
Size: 5698
SalI G'TCGA_C
Cuts at: 1936 3040 1936
Size: 1104 4594
SapI GCTCTTCn'nnn_
Cuts at: 2572 2572
Size: 5698
SbfI CC_TGCA'GG
Cuts at: 3050 3050
Size: 5698
ScaI AGT'ACT
Cuts at: 3946 5658 3946
Size: 1712 3986
SfcI C'TryA_G
Cuts at: 826 1045 2756 3046 4181 4862 5053 826
Size: 219 1711 290 1135 681 191 1471
Fragments arranged by size:
1711 1471 1135 681 290 219 191
SfoI GGC'GCC
Cuts at: 2305 2305
Size: 5698
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5698
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5698
SmaI CCC'GGG
Cuts at: 1872 3025 1872
Size: 1153 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3802 4703 4965 820
Size: 197 926 1859 901 262 1553
Fragments arranged by size:
1859 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3489 493
Size: 2996 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5698
SphI G_CATG'C
Cuts at: 1132 3056 1132
Size: 1924 3774
SspI AAT'ATT
Cuts at: 5 52 3622 5
Size: 47 3570 2081
Fragments arranged by size:
3570 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5698
StyI C'CwwG_G
Cuts at: 513 1065 2004 2115 3137 513
Size: 552 939 111 1022 3074
Fragments arranged by size:
3074 1022 939 552 111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3725 3742 3895 4080 4525 325
Size: 1255 2145 17 153 185 445 1498
Fragments arranged by size:
2145 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3944 5656 96
Size: 274 80 33 51 1443 1967 1712 138
Fragments arranged by size:
1967 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5658 5690 5658
Size: 32 5666
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5698
XbaI T'CTAG_A
Cuts at: 3034 3034
Size: 5698
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2446 2446
Size: 5698
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5698
XmaI C'CCGG_G
Cuts at: 1870 3023 1870
Size: 1153 4545
XmnI GAAnn'nnTTC
Cuts at: 3827 3827
Size: 5698
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3506 276
Size: 53 83 186 936 1972 2468
Fragments arranged by size:
2468 1972 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I
EcoRV FalI FseI FspAI NruI NsiI PacI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SexAI
SfiI SrfI SwaI