Restriction map for
pFN21AB7910
(Circular) (Six-base) MAPSORT of: pFN21AB7910.seq Check: 2991 from: 1 to: 5695
pFN21AB7910 5695 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2727 2727
Size: 5695
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3505 278
Size: 53 83 186 936 1969 2468
Fragments arranged by size:
2468 1969 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3038 1937
Size: 1101 4594
Acc65I G'GTAC_C
Cuts at: 3016 3016
Size: 5695
AclI AA'CG_TT
Cuts at: 1563 3822 4195 1563
Size: 2259 373 3063
Fragments arranged by size:
3063 2259 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2443 2842 2899 3758 5142 5410 1632
Size: 811 399 57 859 1384 268 1917
Fragments arranged by size:
1917 1384 859 811 399 268 57
AfeI AGC'GCT
Cuts at: 2696 2696
Size: 5695
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5498
AflIII A'CryG_T
Cuts at: 1203 4594 5571 1203
Size: 3391 977 1327
Fragments arranged by size:
3391 1327 977
AgeI A'CCGG_T
Cuts at: 4526 4526
Size: 5695
AhdI GACnn_n'nnGTC
Cuts at: 4424 4424
Size: 5695
AleI CACnn'nnGTG
Cuts at: 1166 2817 1166
Size: 1651 4044
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5695
AlwNI CAG_nnn'CTG
Cuts at: 2167 3438 5010 2167
Size: 1271 1572 2852
Fragments arranged by size:
2852 1572 1271
ApaI G_GGCC'C
Cuts at: 2249 2249
Size: 5695
ApaLI G'TGCA_C
Cuts at: 2929 3752 4908 2929
Size: 823 1156 3716
Fragments arranged by size:
3716 1156 823
ApoI r'AATT_y
Cuts at: 1493 3004 3230 3382 5491 1493
Size: 1511 226 152 2109 1697
Fragments arranged by size:
2109 1697 1511 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4249 160
Size: 872 3217 1606
Fragments arranged by size:
3217 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5695
AvaI C'yCGr_G
Cuts at: 1870 1943 3020 1870
Size: 73 1077 4545
Fragments arranged by size:
4545 1077 73
BamHI G'GATC_C
Cuts at: 3025 3392 3025
Size: 367 5328
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2104 2236 2967 3016 4471
Size: 325 217 627 317 132 731 49 1455
Cuts at: 4471 618
Size: 1842
Fragments arranged by size:
1842 1455 731 627 325 317 217 132
49
BanII G_rGCy'C
Cuts at: 1391 2249 2448 2538 2870 3014 1391
Size: 858 199 90 332 144 4072
Fragments arranged by size:
4072 858 332 199 144 90
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2509 928
Size: 971 610 4114
Fragments arranged by size:
4114 971 610
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2587 2621 3886 3920 1903
Size: 34 50 34 566 34 1265 34 3678
Fragments arranged by size:
3678 1265 566 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3587 4797 3587
Size: 1210 4485
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5695
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2228 2418 4306
Size: 107 122 71 631 605 556 190 1888
Cuts at: 4306 136
Size: 1525
Fragments arranged by size:
1888 1525 631 605 556 190 122 107
71
BglII A'GATC_T
Cuts at: 5690 5690
Size: 5695
BlpI GC'TnA_GC
Cuts at: 3112 3112
Size: 5695
Bme1580I G_kGCm'C
Cuts at: 1790 2109 2249 2436 2933 3756 4912 1790
Size: 319 140 187 497 823 1156 2573
Fragments arranged by size:
2573 1156 823 497 319 187 140
BmgBI CAC'GTC
Cuts at: 1318 2641 1318
Size: 1323 4372
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3426 4384 442
Size: 416 53 83 399 24 2009 958 1753
Fragments arranged by size:
2009 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5695
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2222 2417 2645 4355 1239
Size: 983 195 228 1710 2579
Fragments arranged by size:
2579 1710 983 228 195
Bpu10I CC'TnA_GC
Cuts at: 2141 2141
Size: 5695
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2975 3820 4685 4983 2975
Size: 845 865 298 3687
Fragments arranged by size:
3687 865 845 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2106 4358 882
Size: 617 607 2252 2219
Fragments arranged by size:
2252 2219 617 607
BsaAI yAC'GTr
Cuts at: 493 1204 3486 493
Size: 711 2282 2702
Fragments arranged by size:
2702 2282 711
BsaBI GATnn'nnATC
Cuts at: 5689 5689
Size: 5695
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3502 3884 4519 275
Size: 53 83 186 936 1969 382 635 1451
Fragments arranged by size:
1969 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4128 4526 4800 4947 5358 4128
Size: 398 274 147 411 4465
Fragments arranged by size:
4465 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2630 2660 1178
Size: 30 165 30 1227 30 4213
Fragments arranged by size:
4213 1227 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5695
BseYI C'CCAG_C
Cuts at: 2435 4898 5394 2435
Size: 2463 496 2736
Fragments arranged by size:
2736 2463 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2084 1163
Size: 573 348 4774
Fragments arranged by size:
4774 573 348
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3145 3906 4934 1540
Size: 275 186 1144 761 1028 2301
Fragments arranged by size:
2301 1144 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2403 2870 2933 3756 3841 4912 2403
Size: 467 63 823 85 1071 3186
Fragments arranged by size:
3186 1071 823 467 85 63
BsmI GAATG_Cn'
Cuts at: 3206 3299 3206
Size: 93 5602
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2109 2249 2403 2436 2448 2538 2870
Size: 399 319 140 154 33 12 90 332
Cuts at: 2870 2933 3014 3756 3841 4912 1391
Size: 63 81 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 332 319 154 140
90 85 81 63 33 12
BspEI T'CCGG_A
Cuts at: 5358 5358
Size: 5695
BspHI T'CATG_A
Cuts at: 3584 5314 3584
Size: 1730 3965
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2727 2967 3050 844
Size: 1883 240 83 3489
Fragments arranged by size:
3489 1883 240 83
BsrBI CCG'CTC
Cuts at: 1394 1823 2080 3582 1394
Size: 429 257 1502 3507
Fragments arranged by size:
3507 1502 429 257
BsrDI GCAATG_nn'
Cuts at: 65 1248 4190 4364 65
Size: 1183 2942 174 1396
Fragments arranged by size:
2942 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2361 4339 4526 1700
Size: 110 551 1978 187 2869
Fragments arranged by size:
2869 1978 551 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5695
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5695
BssSI C'ACGA_G
Cuts at: 3755 4767 3755
Size: 1012 4683
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5695
BstBI TT'CG_AA
Cuts at: 4499 4499
Size: 5695
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5695
BstYI r'GATC_y
Cuts at: 1972 3025 3392 3777 3794 5246 5332 5344 5690
Size: 1053 367 385 17 1452 86 12 346
Cuts at: 5690 1972
Size: 1977
Fragments arranged by size:
1977 1452 1053 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5143
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4956
Fragments arranged by size:
4956 683 56
BtsI GCAGTG_nn'
Cuts at: 3211 4005 4025 3211
Size: 794 20 4881
Fragments arranged by size:
4881 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5695
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5660
DraI TTT'AAA
Cuts at: 2999 2999
Size: 5695
DraIII CAC_nnn'GTG
Cuts at: 2500 2617 2500
Size: 117 5578
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4702 809
Size: 507 3386 1802
Fragments arranged by size:
3386 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2220 2337 2350
Size: 54 1425 50 275 18 390 117 13
Cuts at: 2350 2608 3142 4031 5660 8
Size: 258 534 889 1629 43
Fragments arranged by size:
1629 1425 889 534 390 275 258 117
54 50 43 18 13
EagI C'GGCC_G
Cuts at: 1537 1812 3142 1537
Size: 275 1330 4090
Fragments arranged by size:
4090 1330 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2021 3625 1353
Size: 668 1604 3423
Fragments arranged by size:
3423 1604 668
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4282 4656 4802 387
Size: 1064 138 2693 374 146 1280
Fragments arranged by size:
2693 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2868 2868
Size: 5695
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2222 2417 2443 2645 2842 2899 3758
Size: 393 590 195 26 202 197 57 859
Cuts at: 3758 4355 5142 5410 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 859 787 597 590 393 268 202
197 195 57 26
EcoO109I rG'GnC_Cy
Cuts at: 2245 2246 2368 2568 2245
Size: 1 122 200 5372
Fragments arranged by size:
5372 200 122 1
EcoRI G'AATT_C
Cuts at: 3004 3382 3004
Size: 378 5317
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2335 2335
Size: 5695
FspI TGC'GCA
Cuts at: 4201 4201
Size: 5695
HaeII r_GCGC'y
Cuts at: 1678 2698 4842 1678
Size: 1020 2144 2531
Fragments arranged by size:
2531 2144 1020
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2264 2296 4336 4368 4410
Size: 32 135 32 692 32 2040 32 42
Cuts at: 4410 4442 1373
Size: 32 2626
Fragments arranged by size:
2626 2040 692 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3039 3285 669
Size: 988 281 1101 246 3079
Fragments arranged by size:
3079 1101 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5695
HpaI GTT'AAC
Cuts at: 3285 3285
Size: 5695
KpnI G_GTAC'C
Cuts at: 3020 3020
Size: 5695
MfeI C'AATT_G
Cuts at: 3294 3294
Size: 5695
MluI A'CGCG_T
Cuts at: 5571 5571
Size: 5695
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2316 2811 2895 4809 4993 1305
Size: 1011 495 84 1914 184 2007
Fragments arranged by size:
2007 1914 1011 495 184 84
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2222 2339 2352 10
Size: 54 1425 343 390 117 13 3353
Fragments arranged by size:
3353 1425 390 343 117 54 13
MslI CAynn'nnrTG
Cuts at: 518 1166 2817 3653 4012 4171 518
Size: 648 1651 836 359 159 2042
Fragments arranged by size:
2042 1651 836 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2919 3111 3788 4936 5181 1606
Size: 1313 192 677 1148 245 2120
Fragments arranged by size:
2120 1313 1148 677 245 192
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5143
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5695
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5695
NotI GC'GGCC_GC
Cuts at: 3142 3142
Size: 5695
NspI r_CATG'y
Cuts at: 1132 1229 2549 2677 3053 4598 1132
Size: 97 1320 128 376 1545 2229
Fragments arranged by size:
2229 1545 1320 376 128 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5695
PciI A'CATG_T
Cuts at: 4594 4594
Size: 5695
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2167 2218 1108
Size: 1059 51 4585
Fragments arranged by size:
4585 1059 51
PfoI T'CCnGG_A
Cuts at: 2394 2394
Size: 5695
PmeI GTTT'AAAC
Cuts at: 2999 2999
Size: 5695
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3764 3796 5277 5309 3764
Size: 32 1481 32 4150
Fragments arranged by size:
4150 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2568 2568
Size: 5695
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5695
PsiI TTA'TAA
Cuts at: 3265 3265
Size: 5695
PspOMI G'GGCC_C
Cuts at: 2245 2245
Size: 5695
PstI C_TGCA'G
Cuts at: 830 3047 830
Size: 2217 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5695
PvuII CAG'CTG
Cuts at: 2919 2919
Size: 5695
SacI G_AGCT'C
Cuts at: 2870 2870
Size: 5695
SalI G'TCGA_C
Cuts at: 1936 3037 1936
Size: 1101 4594
SanDI GG'GwC_CC
Cuts at: 2568 2568
Size: 5695
SapI GCTCTTCn'nnn_
Cuts at: 2021 2021
Size: 5695
SbfI CC_TGCA'GG
Cuts at: 3047 3047
Size: 5695
ScaI AGT'ACT
Cuts at: 3943 5655 3943
Size: 1712 3983
SfcI C'TryA_G
Cuts at: 826 1045 3043 4178 4859 5050 826
Size: 219 1998 1135 681 191 1471
Fragments arranged by size:
1998 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2228 2228
Size: 5695
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5695
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5695
SmaI CCC'GGG
Cuts at: 1872 3022 1872
Size: 1150 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2990 3799 4700 4962 820
Size: 197 926 1047 809 901 262 1553
Fragments arranged by size:
1553 1047 926 901 809 262 197
SnaBI TAC'GTA
Cuts at: 493 3486 493
Size: 2993 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5695
SphI G_CATG'C
Cuts at: 1132 3053 1132
Size: 1921 3774
SspI AAT'ATT
Cuts at: 5 52 3619 5
Size: 47 3567 2081
Fragments arranged by size:
3567 2081 47
StuI AGG'CCT
Cuts at: 1517 2687 2855 1517
Size: 1170 168 4357
Fragments arranged by size:
4357 1170 168
StyI C'CwwG_G
Cuts at: 513 1065 2334 2451 3134 513
Size: 552 1269 117 683 3074
Fragments arranged by size:
3074 1269 683 552 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2729 2897 3722 3739 3892 4077 4522
Size: 1255 1149 168 825 17 153 185 445
Cuts at: 4522 325
Size: 1498
Fragments arranged by size:
1498 1255 1149 825 445 185 168 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3941 5653 96
Size: 274 80 33 51 1443 1964 1712 138
Fragments arranged by size:
1964 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2001 2033 2630 2662 2768 2800 5655 5687 2001
Size: 32 597 32 106 32 2855 32 2009
Fragments arranged by size:
2855 2009 597 106 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5695
XbaI T'CTAG_A
Cuts at: 3031 3031
Size: 5695
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5695
XmaI C'CCGG_G
Cuts at: 1870 3020 1870
Size: 1150 4545
XmnI GAAnn'nnTTC
Cuts at: 3824 3824
Size: 5695
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3503 276
Size: 53 83 186 936 1969 2468
Fragments arranged by size:
2468 1969 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI RsrII SacII SexAI SfoI SrfI
SwaI XcmI