Restriction map for
pFN21AB7904
(Circular) (Six-base) MAPSORT of: pFN21AB7904.seq Check: 1873 from: 1 to: 5689
pFN21AB7904 5689 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3499 278
Size: 53 83 186 936 1963 2468
Fragments arranged by size:
2468 1963 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2602 3032 1937
Size: 665 430 4594
Fragments arranged by size:
4594 665 430
Acc65I G'GTAC_C
Cuts at: 3010 3010
Size: 5689
AclI AA'CG_TT
Cuts at: 1563 2024 3816 4189 1563
Size: 461 1792 373 3063
Fragments arranged by size:
3063 1792 461 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2259 2298 2773 3752 5136 5404 1632
Size: 627 39 475 979 1384 268 1917
Fragments arranged by size:
1917 1384 979 627 475 268 39
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5492
AflIII A'CryG_T
Cuts at: 1203 2337 4588 5565 1203
Size: 1134 2251 977 1327
Fragments arranged by size:
2251 1327 1134 977
AgeI A'CCGG_T
Cuts at: 4520 4520
Size: 5689
AhdI GACnn_n'nnGTC
Cuts at: 4418 4418
Size: 5689
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5689
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5689
AlwNI CAG_nnn'CTG
Cuts at: 3432 5004 3432
Size: 1572 4117
ApaLI G'TGCA_C
Cuts at: 3746 4902 3746
Size: 1156 4533
ApoI r'AATT_y
Cuts at: 1493 2588 2633 2998 3224 3376 5485 1493
Size: 1095 45 365 226 152 2109 1697
Fragments arranged by size:
2109 1697 1095 365 226 152 45
AseI AT'TA_AT
Cuts at: 160 1032 2217 4243 160
Size: 872 1185 2026 1606
Fragments arranged by size:
2026 1606 1185 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
AvaI C'yCGr_G
Cuts at: 1870 1943 3014 1870
Size: 73 1071 4545
Fragments arranged by size:
4545 1071 73
BamHI G'GATC_C
Cuts at: 3019 3386 3019
Size: 367 5322
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3010 4465 618
Size: 325 217 627 1223 1455 1842
Fragments arranged by size:
1842 1455 1223 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3008 1391
Size: 1617 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2329 2406 928
Size: 971 430 77 4211
Fragments arranged by size:
4211 971 430 77
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3880 3914 1903
Size: 34 1943 34 3678
Fragments arranged by size:
3678 1943 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3581 4791 3581
Size: 1210 4479
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5689
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4300 136
Size: 107 122 71 631 605 2628 1525
Fragments arranged by size:
2628 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2281 5684 2281
Size: 3403 2286
BlpI GC'TnA_GC
Cuts at: 3106 3106
Size: 5689
Bme1580I G_kGCm'C
Cuts at: 1790 3750 4906 1790
Size: 1960 1156 2573
Fragments arranged by size:
2573 1960 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5689
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3420 4378 442
Size: 416 53 83 399 24 2003 958 1753
Fragments arranged by size:
2003 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5689
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4349 1239
Size: 3110 2579
Bpu10I CC'TnA_GC
Cuts at: 2448 2448
Size: 5689
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3814 4679 4977 3814
Size: 865 298 4526
Fragments arranged by size:
4526 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4352 882
Size: 617 2853 2219
Fragments arranged by size:
2853 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3480 493
Size: 711 2276 2702
Fragments arranged by size:
2702 2276 711
BsaBI GATnn'nnATC
Cuts at: 5683 5683
Size: 5689
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3496 3878 4513 275
Size: 53 83 186 936 1963 382 635 1451
Fragments arranged by size:
1963 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4122 4520 4794 4941 5352 4122
Size: 398 274 147 411 4459
Fragments arranged by size:
4459 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2250 2280 1178
Size: 30 165 30 847 30 4587
Fragments arranged by size:
4587 847 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5689
BseYI C'CCAG_C
Cuts at: 4892 5388 4892
Size: 496 5193
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5116
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3139 3900 4928 1540
Size: 275 186 1138 761 1028 2301
Fragments arranged by size:
2301 1138 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2843 3750 3835 4906 2843
Size: 907 85 1071 3626
Fragments arranged by size:
3626 1071 907 85
BsmI GAATG_Cn'
Cuts at: 3200 3293 3200
Size: 93 5596
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2843 3008 3750 3835 4906 1391
Size: 399 1053 165 742 85 1071 2174
Fragments arranged by size:
2174 1071 1053 742 399 165 85
BspEI T'CCGG_A
Cuts at: 5352 5352
Size: 5689
BspHI T'CATG_A
Cuts at: 2170 3578 5308 2170
Size: 1408 1730 2551
Fragments arranged by size:
2551 1730 1408
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2127 3044 844
Size: 1283 917 3489
Fragments arranged by size:
3489 1283 917
BsrBI CCG'CTC
Cuts at: 1394 1823 3576 1394
Size: 429 1753 3507
Fragments arranged by size:
3507 1753 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2499 2694 4184 4358 65
Size: 1183 1251 195 1490 174 1396
Fragments arranged by size:
1490 1396 1251 1183 195 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4333 4520 1700
Size: 110 2523 187 2869
Fragments arranged by size:
2869 2523 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5689
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5689
BssSI C'ACGA_G
Cuts at: 3749 4761 3749
Size: 1012 4677
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5689
BstBI TT'CG_AA
Cuts at: 4493 4493
Size: 5689
BstEII G'GTnAC_C
Cuts at: 1184 2767 1184
Size: 1583 4106
BstXI CCAn_nnnn'nTGG
Cuts at: 2927 2927
Size: 5689
BstYI r'GATC_y
Cuts at: 1972 2281 2737 3019 3386 3771 3788 5240 5326
Size: 309 456 282 367 385 17 1452 86
Cuts at: 5326 5338 5684 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 456 385 367 346 309 282
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4198
Fragments arranged by size:
4198 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2886 487
Size: 683 56 1660 3290
Fragments arranged by size:
3290 1660 683 56
BtsI GCAGTG_nn'
Cuts at: 2117 2841 3205 3999 4019 2117
Size: 724 364 794 20 3787
Fragments arranged by size:
3787 794 724 364 20
ClaI AT'CG_AT
Cuts at: 1554 2379 1554
Size: 825 4864
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2392 2427 529
Size: 35 1828 35 3791
Fragments arranged by size:
3791 1828 35 35
DraI TTT'AAA
Cuts at: 2993 2993
Size: 5689
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4696 809
Size: 507 3380 1802
Fragments arranged by size:
3380 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3136 4025 5654
Size: 54 1425 50 275 18 1306 889 1629
Cuts at: 5654 8
Size: 43
Fragments arranged by size:
1629 1425 1306 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3136 1537
Size: 275 1324 4090
Fragments arranged by size:
4090 1324 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2172 2751 3619 1353
Size: 819 579 868 3423
Fragments arranged by size:
3423 868 819 579
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4276 4650 4796 387
Size: 1064 138 2687 374 146 1280
Fragments arranged by size:
2687 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2259 2298 2773 3752 4349 5136 5404
Size: 393 627 39 475 979 597 787 268
Cuts at: 5404 1239
Size: 1524
Fragments arranged by size:
1524 979 787 627 597 475 393 268
39
EcoNI CCTnn'n_nnAGG
Cuts at: 2959 2959
Size: 5689
EcoO109I rG'GnC_Cy
Cuts at: 2782 2782
Size: 5689
EcoRI G'AATT_C
Cuts at: 2588 2998 3376 2588
Size: 410 378 4901
Fragments arranged by size:
4901 410 378
FspI TGC'GCA
Cuts at: 2300 4195 2300
Size: 1895 3794
HaeII r_GCGC'y
Cuts at: 1678 4836 1678
Size: 3158 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2342 2374 4330 4362 4404
Size: 32 135 32 770 32 1956 32 42
Cuts at: 4404 4436 1373
Size: 32 2626
Fragments arranged by size:
2626 1956 770 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2603 3033 3279 669
Size: 988 281 665 430 246 3079
Fragments arranged by size:
3079 988 665 430 281 246
HindIII A'AGCT_T
Cuts at: 748 2625 748
Size: 1877 3812
HpaI GTT'AAC
Cuts at: 3279 3279
Size: 5689
KpnI G_GTAC'C
Cuts at: 3014 3014
Size: 5689
MfeI C'AATT_G
Cuts at: 3288 3288
Size: 5689
MluI A'CGCG_T
Cuts at: 5565 5565
Size: 5689
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2628 4803 4987 1305
Size: 1323 2175 184 2007
Fragments arranged by size:
2175 2007 1323 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3867
Fragments arranged by size:
3867 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2871 3647 4006 4165 518
Size: 648 1705 776 359 159 2042
Fragments arranged by size:
2042 1705 776 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2251 3105 3782 4930 5175 1606
Size: 645 854 677 1148 245 2120
Fragments arranged by size:
2120 1148 854 677 645 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4198
Fragments arranged by size:
4198 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5689
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5689
NotI GC'GGCC_GC
Cuts at: 3136 3136
Size: 5689
NspI r_CATG'y
Cuts at: 1132 1229 2341 3047 4592 1132
Size: 97 1112 706 1545 2229
Fragments arranged by size:
2229 1545 1112 706 97
PacI TTA_AT'TAA
Cuts at: 2217 2217
Size: 5689
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5689
PciI A'CATG_T
Cuts at: 2337 4588 2337
Size: 2251 3438
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5689
PmeI GTTT'AAAC
Cuts at: 2993 2993
Size: 5689
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3758 3790 5271 5303 3758
Size: 32 1481 32 4144
Fragments arranged by size:
4144 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2782 2782
Size: 5689
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5689
PsiI TTA'TAA
Cuts at: 3259 3259
Size: 5689
PstI C_TGCA'G
Cuts at: 830 2657 2848 3041 830
Size: 1827 191 193 3478
Fragments arranged by size:
3478 1827 193 191
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5689
PvuII CAG'CTG
Cuts at: 2251 2251
Size: 5689
SalI G'TCGA_C
Cuts at: 1936 2601 3031 1936
Size: 665 430 4594
Fragments arranged by size:
4594 665 430
SanDI GG'GwC_CC
Cuts at: 2782 2782
Size: 5689
SapI GCTCTTCn'nnn_
Cuts at: 2751 2751
Size: 5689
SbfI CC_TGCA'GG
Cuts at: 3041 3041
Size: 5689
ScaI AGT'ACT
Cuts at: 3937 5649 3937
Size: 1712 3977
SfcI C'TryA_G
Cuts at: 826 1045 2064 2653 2844 2914 3037 4172 4853
Size: 219 1019 589 191 70 123 1135 681
Cuts at: 4853 5044 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1019 681 589 219 191 191
123 70
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5689
SmaI CCC'GGG
Cuts at: 1872 3016 1872
Size: 1144 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3793 4694 4956 820
Size: 197 926 1850 901 262 1553
Fragments arranged by size:
1850 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3480 493
Size: 2987 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5689
SphI G_CATG'C
Cuts at: 1132 3047 1132
Size: 1915 3774
SspI AAT'ATT
Cuts at: 5 52 3613 5
Size: 47 3561 2081
Fragments arranged by size:
3561 2081 47
StuI AGG'CCT
Cuts at: 1517 2164 1517
Size: 647 5042
StyI C'CwwG_G
Cuts at: 513 1065 2004 2772 3128 513
Size: 552 939 768 356 3074
Fragments arranged by size:
3074 939 768 552 356
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3716 3733 3886 4071 4516 325
Size: 1255 2136 17 153 185 445 1498
Fragments arranged by size:
2136 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3935 5647 96
Size: 274 80 33 51 1443 1958 1712 138
Fragments arranged by size:
1958 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5649 5681 5649
Size: 32 5657
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5689
XbaI T'CTAG_A
Cuts at: 3025 3025
Size: 5689
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5689
XmaI C'CCGG_G
Cuts at: 1870 3014 1870
Size: 1144 4545
XmnI GAAnn'nnTTC
Cuts at: 2283 3818 2283
Size: 1535 4154
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3497 276
Size: 53 83 186 936 1963 2468
Fragments arranged by size:
2468 1963 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PacI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PfoI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI
XcmI