Restriction map for
pFN21AB7894
(Circular) (Six-base) MAPSORT of: pFN21AB7894.seq Check: 4813 from: 1 to: 6673
pFN21AB7894 6673 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3545 4483 278
Size: 53 83 186 936 2009 938 2468
Fragments arranged by size:
2468 2009 938 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4016 1937
Size: 2079 4594
Acc65I G'GTAC_C
Cuts at: 3994 3994
Size: 6673
AclI AA'CG_TT
Cuts at: 1563 4800 5173 1563
Size: 3237 373 3063
Fragments arranged by size:
3237 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3291 3320 4736 6120 6388 1632
Size: 1659 29 1416 1384 268 1917
Fragments arranged by size:
1917 1659 1416 1384 268 29
AfeI AGC'GCT
Cuts at: 3515 3515
Size: 6673
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6476
AflIII A'CryG_T
Cuts at: 1203 3490 5572 6549 1203
Size: 2287 2082 977 1327
Fragments arranged by size:
2287 2082 1327 977
AgeI A'CCGG_T
Cuts at: 5504 5504
Size: 6673
AhdI GACnn_n'nnGTC
Cuts at: 2056 3112 5402 2056
Size: 1056 2290 3327
Fragments arranged by size:
3327 2290 1056
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6673
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3305 3336 1184
Size: 2121 31 4521
Fragments arranged by size:
4521 2121 31
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3339 3371 3339
Size: 32 6641
AlwNI CAG_nnn'CTG
Cuts at: 3130 3646 4416 5988 3130
Size: 516 770 1572 3815
Fragments arranged by size:
3815 1572 770 516
ApaI G_GGCC'C
Cuts at: 2591 2641 3138 2591
Size: 50 497 6126
Fragments arranged by size:
6126 497 50
ApaLI G'TGCA_C
Cuts at: 4730 5886 4730
Size: 1156 5517
ApoI r'AATT_y
Cuts at: 1493 3982 4208 4360 6469 1493
Size: 2489 226 152 2109 1697
Fragments arranged by size:
2489 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5227 160
Size: 872 4195 1606
Fragments arranged by size:
4195 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6673
AvaI C'yCGr_G
Cuts at: 1870 1943 2310 2403 2640 3653 3998 1870
Size: 73 367 93 237 1013 345 4545
Fragments arranged by size:
4545 1013 367 345 237 93 73
BamHI G'GATC_C
Cuts at: 2863 4003 4370 2863
Size: 1140 367 5166
Fragments arranged by size:
5166 1140 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3474 3699 3994 5449 618
Size: 325 217 627 1687 225 295 1455 1842
Fragments arranged by size:
1842 1687 1455 627 325 295 225 217
BanII G_rGCy'C
Cuts at: 1391 2310 2411 2591 2641 3138 3260 3729 3992
Size: 919 101 180 50 497 122 469 263
Cuts at: 3992 1391
Size: 4072
Fragments arranged by size:
4072 919 497 469 263 180 122 101
50
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5702
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4864 4898 1903
Size: 34 2927 34 3678
Fragments arranged by size:
3678 2927 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4565 5775 4565
Size: 1210 5463
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6673
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2713 5284 136
Size: 107 122 71 631 605 1041 2571 1525
Fragments arranged by size:
2571 1525 1041 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3453 6668 3453
Size: 3215 3458
BlpI GC'TnA_GC
Cuts at: 4090 4090
Size: 6673
Bme1580I G_kGCm'C
Cuts at: 1790 2591 2641 3138 3325 3477 3653 3704 4734
Size: 801 50 497 187 152 176 51 1030
Cuts at: 4734 5890 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1030 801 497 187 176 152
51 50
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6673
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3118 4404 5362
Size: 416 53 83 399 24 1701 1286 958
Cuts at: 5362 442
Size: 1753
Fragments arranged by size:
1753 1701 1286 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6673
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2857 2857
Size: 6673
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2019 3623 3943 5333 1239
Size: 780 1604 320 1390 2579
Fragments arranged by size:
2579 1604 1390 780 320
Bpu10I CC'TnA_GC
Cuts at: 2832 2832
Size: 6673
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3386 4798 5663 5961 3386
Size: 1412 865 298 4098
Fragments arranged by size:
4098 1412 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5336 882
Size: 617 3837 2219
Fragments arranged by size:
3837 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3383 3491 3713 4464 493
Size: 711 2179 108 222 751 2702
Fragments arranged by size:
2702 2179 751 711 222 108
BsaBI GATnn'nnATC
Cuts at: 6667 6667
Size: 6673
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2684 2789 3542 4480
Size: 53 83 186 936 1151 105 753 938
Cuts at: 4480 4862 5497 275
Size: 382 635 1451
Fragments arranged by size:
1451 1151 938 936 753 635 382 186
105 83 53
BsaWI w'CCGG_w
Cuts at: 2104 2169 5106 5504 5778 5925 6336 2104
Size: 65 2937 398 274 147 411 2441
Fragments arranged by size:
2937 2441 411 398 274 147 65
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2996 3026 3941 3971 1178
Size: 30 165 30 1593 30 915 30 3880
Fragments arranged by size:
3880 1593 915 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3044 3428 1186
Size: 1858 384 4431
Fragments arranged by size:
4431 1858 384
BseYI C'CCAG_C
Cuts at: 2456 3156 3161 3231 3780 5876 6372 2456
Size: 700 5 70 549 2096 496 2757
Fragments arranged by size:
2757 2096 700 549 496 70 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2242 2910 3899 1163
Size: 573 506 668 989 3937
Fragments arranged by size:
3937 989 668 573 506
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3970 4123 4884 5912 1540
Size: 275 186 1969 153 761 1028 2301
Fragments arranged by size:
2301 1969 1028 761 275 186 153
BsiHKAI G_wGCw'C
Cuts at: 3279 3660 3729 4734 4819 5890 3279
Size: 381 69 1005 85 1071 4062
Fragments arranged by size:
4062 1071 1005 381 85 69
BsmI GAATG_Cn'
Cuts at: 2080 3300 4184 4277 2080
Size: 1220 884 93 4476
Fragments arranged by size:
4476 1220 884 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2310 2411 2591 2641 3138 3260 3279
Size: 399 520 101 180 50 497 122 19
Cuts at: 3279 3325 3477 3653 3660 3704 3729 3992 4734
Size: 46 152 176 7 44 25 263 742
Cuts at: 4734 4819 5890 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 520 497 399 263 180
176 152 122 101 85 50 46 44
25 19 7
BspEI T'CCGG_A
Cuts at: 2169 6336 2169
Size: 4167 2506
BspHI T'CATG_A
Cuts at: 2121 4562 6292 2121
Size: 2441 1730 2502
Fragments arranged by size:
2502 2441 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2364 3456 3960 4028 844
Size: 1520 1092 504 68 3489
Fragments arranged by size:
3489 1520 1092 504 68
BsrBI CCG'CTC
Cuts at: 1394 1823 2278 2293 4560 1394
Size: 429 455 15 2267 3507
Fragments arranged by size:
3507 2267 455 429 15
BsrDI GCAATG_nn'
Cuts at: 65 1248 2149 2293 3264 3871 5168 5342 65
Size: 1183 901 144 971 607 1297 174 1396
Fragments arranged by size:
1396 1297 1183 971 901 607 174 144
BsrFI r'CCGG_y
Cuts at: 1700 1810 2613 2712 5317 5504 1700
Size: 110 803 99 2605 187 2869
Fragments arranged by size:
2869 2605 803 187 110 99
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6673
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6673
BssSI C'ACGA_G
Cuts at: 4733 5745 4733
Size: 1012 5661
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3400 794
Size: 2606 4067
BstBI TT'CG_AA
Cuts at: 5477 5477
Size: 6673
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6673
BstYI r'GATC_y
Cuts at: 1972 2598 2863 3222 3453 4003 4370 4755 4772
Size: 626 265 359 231 550 367 385 17
Cuts at: 4772 6224 6310 6322 6668 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 626 550 385 367 359 346
265 231 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2814 2814
Size: 6673
BtgI C'CryG_G
Cuts at: 513 1065 2004 2155 2908 513
Size: 552 939 151 753 4278
Fragments arranged by size:
4278 939 753 552 151
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5934
Fragments arranged by size:
5934 683 56
BtsI GCAGTG_nn'
Cuts at: 2308 2717 2790 2982 3116 3251 4189 4983 5003
Size: 409 73 192 134 135 938 794 20
Cuts at: 5003 2308
Size: 3978
Fragments arranged by size:
3978 938 794 409 192 135 134 73
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6673
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3553 3588 529
Size: 35 2989 35 3614
Fragments arranged by size:
3614 2989 35 35
DraI TTT'AAA
Cuts at: 3977 3977
Size: 6673
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5680 809
Size: 507 4364 1802
Fragments arranged by size:
4364 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2187 2448 2714
Size: 54 1425 50 275 18 357 261 266
Cuts at: 2714 4120 5009 6638 8
Size: 1406 889 1629 43
Fragments arranged by size:
1629 1425 1406 889 357 275 266 261
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4120 1537
Size: 275 2308 4090
Fragments arranged by size:
4090 2308 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2931 2963 3267 3596 4603 1353
Size: 1578 32 304 329 1007 3423
Fragments arranged by size:
3423 1578 1007 329 304 32
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5260 5634 5780 387
Size: 1064 138 3671 374 146 1280
Fragments arranged by size:
3671 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3727 3727
Size: 6673
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2019 3291 3320 3623 3943 4736 5333
Size: 393 387 1272 29 303 320 793 597
Cuts at: 5333 6120 6388 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1272 793 787 597 393 387 320
303 268 29
EcoNI CCTnn'n_nnAGG
Cuts at: 2876 2876
Size: 6673
EcoO109I rG'GnC_Cy
Cuts at: 2587 2588 2706 2851 2895 3106 3134 3667 2587
Size: 1 118 145 44 211 28 533 5593
Fragments arranged by size:
5593 533 211 145 118 44 28 1
EcoRI G'AATT_C
Cuts at: 3982 4360 3982
Size: 378 6295
FspI TGC'GCA
Cuts at: 3872 5179 3872
Size: 1307 5366
FspAI rTGC'GCAy
Cuts at: 3872 3872
Size: 6673
HaeII r_GCGC'y
Cuts at: 1678 2365 3517 3760 5820 1678
Size: 687 1152 243 2060 2531
Fragments arranged by size:
2531 2060 1152 687 243
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2042 2074 2239 2271 2996
Size: 32 135 32 470 32 165 32 725
Cuts at: 2996 3028 3098 3130 3941 3973 5314 5346 5388
Size: 32 70 32 811 32 1341 32 42
Cuts at: 5388 5420 1373
Size: 32 2626
Fragments arranged by size:
2626 1341 811 725 470 165 135 70
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4017 4263 669
Size: 988 281 2079 246 3079
Fragments arranged by size:
3079 2079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6673
HpaI GTT'AAC
Cuts at: 4263 4263
Size: 6673
KpnI G_GTAC'C
Cuts at: 3998 3998
Size: 6673
MfeI C'AATT_G
Cuts at: 4272 4272
Size: 6673
MluI A'CGCG_T
Cuts at: 6549 6549
Size: 6673
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5787 5971 1305
Size: 4482 184 2007
Fragments arranged by size:
4482 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2189 2450 10
Size: 54 1425 343 357 261 4233
Fragments arranged by size:
4233 1425 357 343 261 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3878 4631 4990 5149 518
Size: 648 2712 753 359 159 2042
Fragments arranged by size:
2712 2042 753 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2632 3683 4089 4766 5914 6159 1606
Size: 1026 1051 406 677 1148 245 2120
Fragments arranged by size:
2120 1148 1051 1026 677 406 245
NaeI GCC'GGC
Cuts at: 2615 2714 2615
Size: 99 6574
NcoI C'CATG_G
Cuts at: 513 1065 2004 2155 2908 513
Size: 552 939 151 753 4278
Fragments arranged by size:
4278 939 753 552 151
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6673
NgoMIV G'CCGG_C
Cuts at: 2613 2712 2613
Size: 99 6574
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6673
NotI GC'GGCC_GC
Cuts at: 4120 4120
Size: 6673
NspI r_CATG'y
Cuts at: 1132 1229 2509 2529 2993 4031 5576 1132
Size: 97 1280 20 464 1038 1545 2229
Fragments arranged by size:
2229 1545 1280 1038 464 97 20
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6673
PciI A'CATG_T
Cuts at: 5572 5572
Size: 6673
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3130 1108
Size: 2022 4651
PfoI T'CCnGG_A
Cuts at: 2199 2949 2199
Size: 750 5923
PmeI GTTT'AAAC
Cuts at: 3977 3977
Size: 6673
PmlI CAC'GTG
Cuts at: 3491 3491
Size: 6673
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4742 4774 6255 6287 4742
Size: 32 1481 32 5128
Fragments arranged by size:
5128 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2851 3106 3667 2851
Size: 255 561 5857
Fragments arranged by size:
5857 561 255
PshAI GACnn'nnGTC
Cuts at: 1613 2547 1613
Size: 934 5739
PsiI TTA'TAA
Cuts at: 4243 4243
Size: 6673
PspOMI G'GGCC_C
Cuts at: 2587 2637 3134 2587
Size: 50 497 6126
Fragments arranged by size:
6126 497 50
PstI C_TGCA'G
Cuts at: 830 2361 3453 4025 830
Size: 1531 1092 572 3478
Fragments arranged by size:
3478 1531 1092 572
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6673
PvuII CAG'CTG
Cuts at: 2632 3683 2632
Size: 1051 5622
SacI G_AGCT'C
Cuts at: 3729 3729
Size: 6673
SalI G'TCGA_C
Cuts at: 1936 4015 1936
Size: 2079 4594
SanDI GG'GwC_CC
Cuts at: 3667 3667
Size: 6673
SapI GCTCTTCn'nnn_
Cuts at: 3267 3267
Size: 6673
SbfI CC_TGCA'GG
Cuts at: 4025 4025
Size: 6673
ScaI AGT'ACT
Cuts at: 2657 4921 6633 2657
Size: 2264 1712 2697
Fragments arranged by size:
2697 2264 1712
SfcI C'TryA_G
Cuts at: 826 1045 2357 2820 3449 4021 5156 5837 6028
Size: 219 1312 463 629 572 1135 681 191
Cuts at: 6028 826
Size: 1471
Fragments arranged by size:
1471 1312 1135 681 629 572 463 219
191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2713 2713
Size: 6673
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6673
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6673
SmaI CCC'GGG
Cuts at: 1872 2312 2642 4000 1872
Size: 440 330 1358 4545
Fragments arranged by size:
4545 1358 440 330
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3365 4777 5678 5940 820
Size: 197 926 1422 1412 901 262 1553
Fragments arranged by size:
1553 1422 1412 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4464 493
Size: 3971 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6673
SphI G_CATG'C
Cuts at: 1132 4031 1132
Size: 2899 3774
SspI AAT'ATT
Cuts at: 5 52 4597 5
Size: 47 4545 2081
Fragments arranged by size:
4545 2081 47
StuI AGG'CCT
Cuts at: 1517 2338 1517
Size: 821 5852
StyI C'CwwG_G
Cuts at: 513 1065 2004 2155 2190 2442 2702 2908 3102
Size: 552 939 151 35 252 260 206 194
Cuts at: 3102 4112 513
Size: 1010 3074
Fragments arranged by size:
3074 1010 939 552 260 252 206 194
151 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2470 2741 3099 4700 4717 4870 5055
Size: 1255 890 271 358 1601 17 153 185
Cuts at: 5055 5500 325
Size: 445 1498
Fragments arranged by size:
1601 1498 1255 890 445 358 271 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2655 4919 6631
Size: 274 80 33 51 1443 678 2264 1712
Cuts at: 6631 96
Size: 138
Fragments arranged by size:
2264 1712 1443 678 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6633 6665 6633
Size: 32 6641
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6673
XbaI T'CTAG_A
Cuts at: 4009 4009
Size: 6673
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3545 3545
Size: 6673
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6673
XmaI C'CCGG_G
Cuts at: 1870 2310 2640 3998 1870
Size: 440 330 1358 4545
Fragments arranged by size:
4545 1358 440 330
XmnI GAAnn'nnTTC
Cuts at: 2579 3300 4802 2579
Size: 721 1502 4450
Fragments arranged by size:
4450 1502 721
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3543 4481 276
Size: 53 83 186 936 2009 938 2468
Fragments arranged by size:
2468 2009 938 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI
BsmBI BstXI BstZ17I DraIII EcoRV FalI FseI KasI
NarI NruI NsiI PacI PspXI PsrI RsrII SacII
SexAI SfoI SrfI SwaI