Restriction map for
pFN21AB7888
(Circular) (Six-base) MAPSORT of: pFN21AB7888.seq Check: 2280 from: 1 to: 6658
pFN21AB7888 6658 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4468 278
Size: 53 83 186 936 2932 2468
Fragments arranged by size:
2932 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3952 4001 1937
Size: 2015 49 4594
Fragments arranged by size:
4594 2015 49
Acc65I G'GTAC_C
Cuts at: 3979 3979
Size: 6658
AclI AA'CG_TT
Cuts at: 1563 4785 5158 1563
Size: 3222 373 3063
Fragments arranged by size:
3222 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2134 2736 3640 4721 6105 6373 1632
Size: 502 602 904 1081 1384 268 1917
Fragments arranged by size:
1917 1384 1081 904 602 502 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6461
AflIII A'CryG_T
Cuts at: 1203 5557 6534 1203
Size: 4354 977 1327
Fragments arranged by size:
4354 1327 977
AgeI A'CCGG_T
Cuts at: 5489 5489
Size: 6658
AhdI GACnn_n'nnGTC
Cuts at: 5387 5387
Size: 6658
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6658
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6658
AlwNI CAG_nnn'CTG
Cuts at: 2122 4401 5973 2122
Size: 2279 1572 2807
Fragments arranged by size:
2807 2279 1572
ApaLI G'TGCA_C
Cuts at: 3195 4715 5871 3195
Size: 1520 1156 3982
Fragments arranged by size:
3982 1520 1156
ApoI r'AATT_y
Cuts at: 1493 2264 2372 2610 2742 2922 3071 3904 3967
Size: 771 108 238 132 180 149 833 63
Cuts at: 3967 4193 4345 6454 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 833 771 238 226 180 152
149 132 108 63
AseI AT'TA_AT
Cuts at: 160 1032 3258 5212 160
Size: 872 2226 1954 1606
Fragments arranged by size:
2226 1954 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6658
AvaI C'yCGr_G
Cuts at: 1870 1943 2940 3983 1870
Size: 73 997 1043 4545
Fragments arranged by size:
4545 1043 997 73
BamHI G'GATC_C
Cuts at: 3373 3988 4355 3373
Size: 615 367 5676
Fragments arranged by size:
5676 615 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2081 2219 3979 5434 618
Size: 325 217 627 294 138 1760 1455 1842
Fragments arranged by size:
1842 1760 1455 627 325 294 217 138
BanII G_rGCy'C
Cuts at: 1391 2168 3977 1391
Size: 777 1809 4072
Fragments arranged by size:
4072 1809 777
BbeI G_GCGC'C
Cuts at: 2223 2223
Size: 6658
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5687
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4849 4883 1903
Size: 34 2912 34 3678
Fragments arranged by size:
3678 2912 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4550 5760 4550
Size: 1210 5448
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6658
BfrBI ATG'CAT
Cuts at: 3701 3701
Size: 6658
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2079 5269 136
Size: 107 122 71 631 605 407 3190 1525
Fragments arranged by size:
3190 1525 631 605 407 122 107 71
BglII A'GATC_T
Cuts at: 2620 2689 6653 2620
Size: 69 3964 2625
Fragments arranged by size:
3964 2625 69
BlpI GC'TnA_GC
Cuts at: 3355 4075 3355
Size: 720 5938
Bme1580I G_kGCm'C
Cuts at: 1790 2320 3199 4719 5875 1790
Size: 530 879 1520 1156 2573
Fragments arranged by size:
2573 1520 1156 879 530
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6658
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4389 5347 442
Size: 416 53 83 399 24 2972 958 1753
Fragments arranged by size:
2972 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6658
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2487 2784 5318 1239
Size: 1248 297 2534 2579
Fragments arranged by size:
2579 2534 1248 297
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3184 3219 4783 5648 5946 3184
Size: 35 1564 865 298 3896
Fragments arranged by size:
3896 1564 865 298 35
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5321 882
Size: 617 3822 2219
Fragments arranged by size:
3822 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4449 493
Size: 711 3245 2702
Fragments arranged by size:
3245 2702 711
BsaBI GATnn'nnATC
Cuts at: 2033 2694 6652 2033
Size: 661 3958 2039
Fragments arranged by size:
3958 2039 661
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2220 4465 4847 5482
Size: 53 83 186 936 687 2245 382 635
Cuts at: 5482 275
Size: 1451
Fragments arranged by size:
2245 1451 936 687 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3094 5091 5489 5763 5910 6321 3094
Size: 1997 398 274 147 411 3431
Fragments arranged by size:
3431 1997 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6433
Fragments arranged by size:
6433 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2186 2189 2192 1186
Size: 1000 3 3 5652
Fragments arranged by size:
5652 1000 3 3
BseYI C'CCAG_C
Cuts at: 2136 5861 6357 2136
Size: 3725 496 2437
Fragments arranged by size:
3725 2437 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2658 3921 1163
Size: 573 922 1263 3900
Fragments arranged by size:
3900 1263 922 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4108 4869 5897 1540
Size: 275 186 2107 761 1028 2301
Fragments arranged by size:
2301 2107 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3199 4719 4804 5875 3199
Size: 1520 85 1071 3982
Fragments arranged by size:
3982 1520 1071 85
BsmI GAATG_Cn'
Cuts at: 4169 4262 4169
Size: 93 6565
BsmBI CGTCTCn'nnnn_
Cuts at: 2081 2081
Size: 6658
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2168 2320 3199 3977 4719 4804 5875
Size: 399 378 152 879 778 742 85 1071
Cuts at: 5875 1391
Size: 2174
Fragments arranged by size:
2174 1071 879 778 742 399 378 152
85
BspEI T'CCGG_A
Cuts at: 3094 6321 3094
Size: 3227 3431
BspHI T'CATG_A
Cuts at: 3174 4547 6277 3174
Size: 1373 1730 3555
Fragments arranged by size:
3555 1730 1373
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2047 2103 4013 844
Size: 1203 56 1910 3489
Fragments arranged by size:
3489 1910 1203 56
BsrBI CCG'CTC
Cuts at: 1394 1823 4545 1394
Size: 429 2722 3507
Fragments arranged by size:
3507 2722 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3095 5153 5327 65
Size: 1183 1847 2058 174 1396
Fragments arranged by size:
2058 1847 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5302 5489 1700
Size: 110 3492 187 2869
Fragments arranged by size:
3492 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3517 96
Size: 3421 3237
BssHII G'CGCG_C
Cuts at: 1925 2017 1925
Size: 92 6566
BssSI C'ACGA_G
Cuts at: 4718 5730 4718
Size: 1012 5646
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3127 794
Size: 2333 4325
BstBI TT'CG_AA
Cuts at: 2593 5462 2593
Size: 2869 3789
BstEII G'GTnAC_C
Cuts at: 1184 2810 1184
Size: 1626 5032
BstXI CCAn_nnnn'nTGG
Cuts at: 3634 3715 3634
Size: 81 6577
BstYI r'GATC_y
Cuts at: 1972 2620 2642 2689 3373 3988 4355 4740 4757
Size: 648 22 47 684 615 367 385 17
Cuts at: 4757 6209 6295 6307 6653 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 684 648 615 385 367 346
86 47 22 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6106
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5919
Fragments arranged by size:
5919 683 56
BtsI GCAGTG_nn'
Cuts at: 2028 4174 4968 4988 2028
Size: 2146 794 20 3698
Fragments arranged by size:
3698 2146 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6658
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6623
DraI TTT'AAA
Cuts at: 3962 3962
Size: 6658
DraIII CAC_nnn'GTG
Cuts at: 2980 2980
Size: 6658
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3228 5665 809
Size: 507 1912 2437 1802
Fragments arranged by size:
2437 1912 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2213 3205 4105
Size: 54 1425 50 275 18 383 992 900
Cuts at: 4105 4994 6623 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 992 900 889 383 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4105 1537
Size: 275 2293 4090
Fragments arranged by size:
4090 2293 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2837 3526 4588 1353
Size: 1484 689 1062 3423
Fragments arranged by size:
3423 1484 1062 689
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5245 5619 5765 387
Size: 1064 138 3656 374 146 1280
Fragments arranged by size:
3656 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2134 2487 2736 2784 3640 4721 5318
Size: 393 502 353 249 48 856 1081 597
Cuts at: 5318 6105 6373 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1081 856 787 597 502 393 353
268 249 48
EcoO109I rG'GnC_Cy
Cuts at: 3230 3230
Size: 6658
EcoRI G'AATT_C
Cuts at: 3967 4345 3967
Size: 378 6280
EcoRV GAT'ATC
Cuts at: 2445 2445
Size: 6658
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3216 3216
Size: 6658
FspI TGC'GCA
Cuts at: 5164 5164
Size: 6658
HaeII r_GCGC'y
Cuts at: 1678 2223 5805 1678
Size: 545 3582 2531
Fragments arranged by size:
3582 2531 545
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2371 2378 2403 2410 2986
Size: 32 135 32 799 7 25 7 576
Cuts at: 2986 3018 3047 3079 3169 3201 3373 3405 5299
Size: 32 29 32 90 32 172 32 1894
Cuts at: 5299 5331 5373 5405 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1894 799 576 172 135 90 42
32 32 32 32 32 32 32 32
29 25 7 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2827 4002 4248 669
Size: 988 281 889 1175 246 3079
Fragments arranged by size:
3079 1175 988 889 281 246
HindIII A'AGCT_T
Cuts at: 748 3237 748
Size: 2489 4169
HpaI GTT'AAC
Cuts at: 2827 4248 2827
Size: 1421 5237
KasI G'GCGC_C
Cuts at: 2219 2219
Size: 6658
KpnI G_GTAC'C
Cuts at: 3983 3983
Size: 6658
MfeI C'AATT_G
Cuts at: 2438 3485 4257 2438
Size: 1047 772 4839
Fragments arranged by size:
4839 1047 772
MluI A'CGCG_T
Cuts at: 6534 6534
Size: 6658
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2581 5772 5956 1305
Size: 1276 3191 184 2007
Fragments arranged by size:
3191 2007 1276 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3207 10
Size: 54 1425 343 1375 3461
Fragments arranged by size:
3461 1425 1375 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2981 3108 3176 3525 3713 3858 4616
Size: 648 1815 127 68 349 188 145 758
Cuts at: 4616 4975 5134 518
Size: 359 159 2042
Fragments arranged by size:
2042 1815 758 648 359 349 188 159
145 127 68
MspA1I CmG'CkG
Cuts at: 1606 2104 2140 4074 4751 5899 6144 1606
Size: 498 36 1934 677 1148 245 2120
Fragments arranged by size:
2120 1934 1148 677 498 245 36
NarI GG'CG_CC
Cuts at: 2220 2220
Size: 6658
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6106
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6658
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6658
NotI GC'GGCC_GC
Cuts at: 4105 4105
Size: 6658
NsiI A_TGCA'T
Cuts at: 3703 3703
Size: 6658
NspI r_CATG'y
Cuts at: 1132 1229 3611 4016 5561 1132
Size: 97 2382 405 1545 2229
Fragments arranged by size:
2382 2229 1545 405 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6658
PciI A'CATG_T
Cuts at: 5557 5557
Size: 6658
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2068 1108
Size: 960 5698
PmeI GTTT'AAAC
Cuts at: 3962 3962
Size: 6658
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4727 4759 6240 6272 4727
Size: 32 1481 32 5113
Fragments arranged by size:
5113 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3230 3230
Size: 6658
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6658
PsiI TTA'TAA
Cuts at: 3915 4228 3915
Size: 313 6345
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3597 3629 3597
Size: 32 6626
PstI C_TGCA'G
Cuts at: 830 4010 830
Size: 3180 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6658
SalI G'TCGA_C
Cuts at: 1936 4000 1936
Size: 2064 4594
SbfI CC_TGCA'GG
Cuts at: 4010 4010
Size: 6658
ScaI AGT'ACT
Cuts at: 3648 4906 6618 3648
Size: 1258 1712 3688
Fragments arranged by size:
3688 1712 1258
SexAI A'CCwGG_T
Cuts at: 2860 3600 2860
Size: 740 5918
SfcI C'TryA_G
Cuts at: 826 1045 2629 3566 3681 4006 5141 5822 6013
Size: 219 1584 937 115 325 1135 681 191
Cuts at: 6013 826
Size: 1471
Fragments arranged by size:
1584 1471 1135 937 681 325 219 191
115
SfoI GGC'GCC
Cuts at: 2221 2221
Size: 6658
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6658
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6658
SmaI CCC'GGG
Cuts at: 1872 3985 1872
Size: 2113 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3163 3234 4762 5663 5925 820
Size: 197 926 1220 71 1528 901 262 1553
Fragments arranged by size:
1553 1528 1220 926 901 262 197 71
SnaBI TAC'GTA
Cuts at: 493 4449 493
Size: 3956 2702
SpeI A'CTAG_T
Cuts at: 152 3955 152
Size: 3803 2855
SphI G_CATG'C
Cuts at: 1132 4016 1132
Size: 2884 3774
SspI AAT'ATT
Cuts at: 5 52 3493 4582 5
Size: 47 3441 1089 2081
Fragments arranged by size:
3441 2081 1089 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6658
StyI C'CwwG_G
Cuts at: 513 1065 2285 3627 4097 513
Size: 552 1220 1342 470 3074
Fragments arranged by size:
3074 1342 1220 552 470
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2207 4685 4702 4855 5040 5485 325
Size: 1255 627 2478 17 153 185 445 1498
Fragments arranged by size:
2478 1498 1255 627 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3517 3646 4904
Size: 274 80 33 51 1443 1540 129 1258
Cuts at: 4904 6616 96
Size: 1712 138
Fragments arranged by size:
1712 1540 1443 1258 274 138 129 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6618 6650 6618
Size: 32 6626
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6658
XbaI T'CTAG_A
Cuts at: 3994 3994
Size: 6658
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6658
XmaI C'CCGG_G
Cuts at: 1870 3983 1870
Size: 2113 4545
XmnI GAAnn'nnTTC
Cuts at: 2949 3282 4787 2949
Size: 333 1505 4820
Fragments arranged by size:
4820 1505 333
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4466 276
Size: 53 83 186 936 2932 2468
Fragments arranged by size:
2932 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI
SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BplI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoNI FseI
FspAI NaeI NgoMIV NruI PacI PfoI PmlI PspOMI
PspXI PvuII RsrII SacI SacII SanDI SapI SfiI
SrfI SwaI XcmI