Restriction map for
pFN21AB7885
(Circular) (Six-base) MAPSORT of: pFN21AB7885.seq Check: 6410 from: 1 to: 6448
pFN21AB7885 6448 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4258 278
Size: 53 83 186 936 2722 2468
Fragments arranged by size:
2722 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3791 1937
Size: 1854 4594
Acc65I G'GTAC_C
Cuts at: 3769 3769
Size: 6448
AclI AA'CG_TT
Cuts at: 1563 4575 4948 1563
Size: 3012 373 3063
Fragments arranged by size:
3063 3012 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2151 3141 3207 4511 5895 6163 1632
Size: 519 990 66 1304 1384 268 1917
Fragments arranged by size:
1917 1384 1304 990 519 268 66
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6251
AflIII A'CryG_T
Cuts at: 1203 3153 5347 6324 1203
Size: 1950 2194 977 1327
Fragments arranged by size:
2194 1950 1327 977
AgeI A'CCGG_T
Cuts at: 5279 5279
Size: 6448
AhdI GACnn_n'nnGTC
Cuts at: 3511 3553 5177 3511
Size: 42 1624 4782
Fragments arranged by size:
4782 1624 42
AleI CACnn'nnGTG
Cuts at: 1166 2514 2621 3189 1166
Size: 1348 107 568 4425
Fragments arranged by size:
4425 1348 568 107
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6448
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2411 2443 2411
Size: 32 6416
AlwNI CAG_nnn'CTG
Cuts at: 2680 3097 3378 4191 5763 2680
Size: 417 281 813 1572 3365
Fragments arranged by size:
3365 1572 813 417 281
ApaLI G'TGCA_C
Cuts at: 2042 2050 4505 5661 2042
Size: 8 2455 1156 2829
Fragments arranged by size:
2829 2455 1156 8
ApoI r'AATT_y
Cuts at: 1493 2277 2710 3022 3281 3494 3757 3983 4135
Size: 784 433 312 259 213 263 226 152
Cuts at: 4135 6244 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 784 433 312 263 259 226
213 152
AseI AT'TA_AT
Cuts at: 160 1032 5002 160
Size: 872 3970 1606
Fragments arranged by size:
3970 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6448
AvaI C'yCGr_G
Cuts at: 1870 1943 3773 1870
Size: 73 1830 4545
Fragments arranged by size:
4545 1830 73
BamHI G'GATC_C
Cuts at: 2245 3778 4145 2245
Size: 1533 367 4548
Fragments arranged by size:
4548 1533 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3309 3769 5224 618
Size: 325 217 627 1522 460 1455 1842
Fragments arranged by size:
1842 1522 1455 627 460 325 217
BanII G_rGCy'C
Cuts at: 1391 3767 1391
Size: 2376 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2075 2447 3464 3515 928
Size: 971 176 372 1017 51 3861
Fragments arranged by size:
3861 1017 971 372 176 51
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4639 4673 1903
Size: 34 2702 34 3678
Fragments arranged by size:
3678 2702 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4340 5550 4340
Size: 1210 5238
BclI T'GATC_A
Cuts at: 1548 2227 1548
Size: 679 5769
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5059 136
Size: 107 122 71 631 605 3387 1525
Fragments arranged by size:
3387 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3113 6443 3113
Size: 3330 3118
BlpI GC'TnA_GC
Cuts at: 3865 3865
Size: 6448
Bme1580I G_kGCm'C
Cuts at: 1790 2046 2054 4509 5665 1790
Size: 256 8 2455 1156 2573
Fragments arranged by size:
2573 2455 1156 256 8
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6448
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3166 4179 5137
Size: 416 53 83 399 24 1749 1013 958
Cuts at: 5137 442
Size: 1753
Fragments arranged by size:
1753 1749 1013 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6448
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3262 3262
Size: 6448
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2147 2801 3440 5108 1239
Size: 908 654 639 1668 2579
Fragments arranged by size:
2579 1668 908 654 639
Bpu10I CC'TnA_GC
Cuts at: 2593 2593
Size: 6448
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2927 3423 4573 5438 5736 2927
Size: 496 1150 865 298 3639
Fragments arranged by size:
3639 1150 865 496 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5111 882
Size: 617 3612 2219
Fragments arranged by size:
3612 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4239 493
Size: 711 3035 2702
Fragments arranged by size:
3035 2702 711
BsaBI GATnn'nnATC
Cuts at: 6442 6442
Size: 6448
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4255 4637 5272 275
Size: 53 83 186 936 2722 382 635 1451
Fragments arranged by size:
2722 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4881 5279 5553 5700 6111 4881
Size: 398 274 147 411 5218
Fragments arranged by size:
5218 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2411 2441 3126 3156 1178
Size: 30 165 30 1008 30 685 30 4470
Fragments arranged by size:
4470 1008 685 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2108 2150 2948 2951 1186
Size: 922 42 798 3 4683
Fragments arranged by size:
4683 922 798 42 3
BseYI C'CCAG_C
Cuts at: 2015 2055 5651 6147 2015
Size: 40 3596 496 2316
Fragments arranged by size:
3596 2316 496 40
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3213 1163
Size: 573 1477 4398
Fragments arranged by size:
4398 1477 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3898 4659 5687 1540
Size: 275 186 1897 761 1028 2301
Fragments arranged by size:
2301 1897 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2046 2054 4509 4594 5665 2046
Size: 8 2455 85 1071 2829
Fragments arranged by size:
2829 2455 1071 85 8
BsmI GAATG_Cn'
Cuts at: 3959 4052 3959
Size: 93 6355
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2046 2054 3767 4509 4594 5665 1391
Size: 399 256 8 1713 742 85 1071 2174
Fragments arranged by size:
2174 1713 1071 742 399 256 85 8
BspEI T'CCGG_A
Cuts at: 6111 6111
Size: 6448
BspHI T'CATG_A
Cuts at: 2632 4337 6067 2632
Size: 1705 1730 3013
Fragments arranged by size:
3013 1730 1705
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3803 844
Size: 2959 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2911 4335 1394
Size: 429 1088 1424 3507
Fragments arranged by size:
3507 1424 1088 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3069 4943 5117 65
Size: 1183 1821 1874 174 1396
Fragments arranged by size:
1874 1821 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5092 5279 1700
Size: 110 3282 187 2869
Fragments arranged by size:
3282 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3319 96
Size: 3223 3225
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6448
BssSI C'ACGA_G
Cuts at: 4508 5520 4508
Size: 1012 5436
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3262 794
Size: 2468 3980
BstBI TT'CG_AA
Cuts at: 2836 5252 2836
Size: 2416 4032
BstEII G'GTnAC_C
Cuts at: 1184 2980 1184
Size: 1796 4652
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 3681 2011
Size: 1670 4778
BstYI r'GATC_y
Cuts at: 1972 2245 3006 3113 3374 3699 3778 4145 4530
Size: 273 761 107 261 325 79 367 385
Cuts at: 4530 4547 5999 6085 6097 6443 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 761 385 367 346 325 273
261 107 86 79 17 12
Bsu36I CC'TnA_GG
Cuts at: 3636 3636
Size: 6448
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4957
Fragments arranged by size:
4957 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5709
Fragments arranged by size:
5709 683 56
BtsI GCAGTG_nn'
Cuts at: 2264 3964 4758 4778 2264
Size: 1700 794 20 3934
Fragments arranged by size:
3934 1700 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6448
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6413
DraI TTT'AAA
Cuts at: 3752 3752
Size: 6448
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5455 809
Size: 507 4139 1802
Fragments arranged by size:
4139 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2403 3895 4784
Size: 54 1425 50 275 18 573 1492 889
Cuts at: 4784 6413 8
Size: 1629 43
Fragments arranged by size:
1629 1492 1425 889 573 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3895 1537
Size: 275 2083 4090
Fragments arranged by size:
4090 2083 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2127 3117 4378 1353
Size: 774 990 1261 3423
Fragments arranged by size:
3423 1261 990 774
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5035 5409 5555 387
Size: 1064 138 3446 374 146 1280
Fragments arranged by size:
3446 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2147 2151 2801 3141 3207 3440 4511
Size: 393 515 4 650 340 66 233 1071
Cuts at: 4511 5108 5895 6163 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1071 787 650 597 515 393 340
268 233 66 4
EcoNI CCTnn'n_nnAGG
Cuts at: 3663 3663
Size: 6448
EcoO109I rG'GnC_Cy
Cuts at: 2597 2740 2841 2597
Size: 143 101 6204
Fragments arranged by size:
6204 143 101
EcoRI G'AATT_C
Cuts at: 2710 3757 4135 2710
Size: 1047 378 5023
Fragments arranged by size:
5023 1047 378
FspI TGC'GCA
Cuts at: 4954 4954
Size: 6448
HaeII r_GCGC'y
Cuts at: 1678 5595 1678
Size: 3917 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2230 2262 2846 2878 3359
Size: 32 135 32 658 32 584 32 481
Cuts at: 3359 3391 3497 3529 3539 3571 5089 5121 5163
Size: 32 106 32 10 32 1518 32 42
Cuts at: 5163 5195 1373
Size: 32 2626
Fragments arranged by size:
2626 1518 658 584 481 135 106 42
32 32 32 32 32 32 32 32
32 10
HincII GTy'rAC
Cuts at: 669 1657 1938 3181 3792 4038 669
Size: 988 281 1243 611 246 3079
Fragments arranged by size:
3079 1243 988 611 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6448
HpaI GTT'AAC
Cuts at: 4038 4038
Size: 6448
KpnI G_GTAC'C
Cuts at: 3773 3773
Size: 6448
MfeI C'AATT_G
Cuts at: 2118 4047 2118
Size: 1929 4519
MluI A'CGCG_T
Cuts at: 6324 6324
Size: 6448
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5562 5746 1305
Size: 4257 184 2007
Fragments arranged by size:
4257 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2405 10
Size: 54 1425 343 573 4053
Fragments arranged by size:
4053 1425 573 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2514 2621 3189 4406 4765 4924
Size: 648 843 505 107 568 1217 359 159
Cuts at: 4924 518
Size: 2042
Fragments arranged by size:
2042 1217 843 648 568 505 359 159
107
MspA1I CmG'CkG
Cuts at: 1606 2038 3864 4541 5689 5934 1606
Size: 432 1826 677 1148 245 2120
Fragments arranged by size:
2120 1826 1148 677 432 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4957
Fragments arranged by size:
4957 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6448
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6448
NotI GC'GGCC_GC
Cuts at: 3895 3895
Size: 6448
NspI r_CATG'y
Cuts at: 1132 1229 3157 3806 5351 1132
Size: 97 1928 649 1545 2229
Fragments arranged by size:
2229 1928 1545 649 97
PasI CC'CwG_GG
Cuts at: 1348 3330 1348
Size: 1982 4466
PciI A'CATG_T
Cuts at: 3153 5347 3153
Size: 2194 4254
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6448
PfoI T'CCnGG_A
Cuts at: 3009 3270 3009
Size: 261 6187
PmeI GTTT'AAAC
Cuts at: 3752 3752
Size: 6448
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2411 2443 4517 4549 6030 6062 2411
Size: 32 2074 32 1481 32 2797
Fragments arranged by size:
2797 2074 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2740 2841 2740
Size: 101 6347
PshAI GACnn'nnGTC
Cuts at: 1613 2846 3591 1613
Size: 1233 745 4470
Fragments arranged by size:
4470 1233 745
PsiI TTA'TAA
Cuts at: 2207 4018 2207
Size: 1811 4637
PstI C_TGCA'G
Cuts at: 830 2380 3800 830
Size: 1550 1420 3478
Fragments arranged by size:
3478 1550 1420
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6448
SalI G'TCGA_C
Cuts at: 1936 3790 1936
Size: 1854 4594
SapI GCTCTTCn'nnn_
Cuts at: 3117 3117
Size: 6448
SbfI CC_TGCA'GG
Cuts at: 3800 3800
Size: 6448
ScaI AGT'ACT
Cuts at: 4696 6408 4696
Size: 1712 4736
SfcI C'TryA_G
Cuts at: 826 1045 2376 3796 4931 5612 5803 826
Size: 219 1331 1420 1135 681 191 1471
Fragments arranged by size:
1471 1420 1331 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6448
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6448
SmaI CCC'GGG
Cuts at: 1872 3775 1872
Size: 1903 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2906 3438 4552 5453 5715 820
Size: 197 926 963 532 1114 901 262 1553
Fragments arranged by size:
1553 1114 963 926 901 532 262 197
SnaBI TAC'GTA
Cuts at: 493 4239 493
Size: 3746 2702
SpeI A'CTAG_T
Cuts at: 152 2828 152
Size: 2676 3772
SphI G_CATG'C
Cuts at: 1132 3806 1132
Size: 2674 3774
SspI AAT'ATT
Cuts at: 5 52 4372 5
Size: 47 4320 2081
Fragments arranged by size:
4320 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6448
StyI C'CwwG_G
Cuts at: 513 1065 2004 2406 2736 3887 513
Size: 552 939 402 330 1151 3074
Fragments arranged by size:
3074 1151 939 552 402 330
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2069 4475 4492 4645 4830 5275 325
Size: 1255 489 2406 17 153 185 445 1498
Fragments arranged by size:
2406 1498 1255 489 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2627 3319 4694
Size: 274 80 33 51 1443 650 692 1375
Cuts at: 4694 6406 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1375 692 650 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6408 6440 6408
Size: 32 6416
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6448
XbaI T'CTAG_A
Cuts at: 3784 3784
Size: 6448
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6448
XmaI C'CCGG_G
Cuts at: 1870 3773 1870
Size: 1903 4545
XmnI GAAnn'nnTTC
Cuts at: 2072 3212 4577 2072
Size: 1140 1365 3943
Fragments arranged by size:
3943 1365 1140
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4256 276
Size: 53 83 186 936 2722 2468
Fragments arranged by size:
2722 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI