Restriction map for
pFN21AB7883
(Circular) (Six-base) MAPSORT of: pFN21AB7883.seq Check: 5034 from: 1 to: 6442
pFN21AB7883 6442 bp
With 182 enzymes: SgfI *
December 12, 2011 16:58 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3258 4252 278
Size: 53 83 186 936 1722 994 2468
Fragments arranged by size:
2468 1722 994 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2787 3785 1937
Size: 850 998 4594
Fragments arranged by size:
4594 998 850
Acc65I G'GTAC_C
Cuts at: 3763 3763
Size: 6442
AclI AA'CG_TT
Cuts at: 1563 4569 4942 1563
Size: 3006 373 3063
Fragments arranged by size:
3063 3006 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2789 3753 4505 5889 6157 1632
Size: 1157 964 752 1384 268 1917
Fragments arranged by size:
1917 1384 1157 964 752 268
AfeI AGC'GCT
Cuts at: 3544 3544
Size: 6442
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6245
AflIII A'CryG_T
Cuts at: 1203 3672 5341 6318 1203
Size: 2469 1669 977 1327
Fragments arranged by size:
2469 1669 1327 977
AgeI A'CCGG_T
Cuts at: 2951 5273 2951
Size: 2322 4120
AhdI GACnn_n'nnGTC
Cuts at: 5171 5171
Size: 6442
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6442
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2510 1184
Size: 1326 5116
AlwNI CAG_nnn'CTG
Cuts at: 2027 3395 4185 5757 2027
Size: 1368 790 1572 2712
Fragments arranged by size:
2712 1572 1368 790
ApaI G_GGCC'C
Cuts at: 2228 2294 2366 2372 3635 2228
Size: 66 72 6 1263 5035
Fragments arranged by size:
5035 1263 72 66 6
ApaLI G'TGCA_C
Cuts at: 2947 3062 4499 5655 2947
Size: 115 1437 1156 3734
Fragments arranged by size:
3734 1437 1156 115
ApoI r'AATT_y
Cuts at: 1493 3751 3977 4129 6238 1493
Size: 2258 226 152 2109 1697
Fragments arranged by size:
2258 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4996 160
Size: 872 3964 1606
Fragments arranged by size:
3964 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6442
AvaI C'yCGr_G
Cuts at: 1870 1943 2227 2365 3628 3635 3724 3767 1870
Size: 73 284 138 1263 7 89 43 4545
Fragments arranged by size:
4545 1263 284 138 89 73 43 7
BamHI G'GATC_C
Cuts at: 2857 3772 4139 2857
Size: 915 367 5160
Fragments arranged by size:
5160 915 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2299 3410 3526 3563 3647
Size: 325 217 627 512 1111 116 37 84
Cuts at: 3647 3763 5218 618
Size: 116 1455 1842
Fragments arranged by size:
1842 1455 1111 627 512 325 217 116
116 84 37
BanII G_rGCy'C
Cuts at: 1391 2228 2294 2366 2372 2511 2877 2967 3126
Size: 837 66 72 6 139 366 90 159
Cuts at: 3126 3438 3635 3761 1391
Size: 312 197 126 4072
Fragments arranged by size:
4072 837 366 312 197 159 139 126
90 72 66 6
BbeI G_GCGC'C
Cuts at: 2303 2303
Size: 6442
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3133 928
Size: 971 1234 4237
Fragments arranged by size:
4237 1234 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3452 3486 4633 4667 1903
Size: 34 50 34 1431 34 1147 34 3678
Fragments arranged by size:
3678 1431 1147 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4334 5544 4334
Size: 1210 5232
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6442
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2337 3663 5053
Size: 107 122 71 631 605 665 1326 1390
Cuts at: 5053 136
Size: 1525
Fragments arranged by size:
1525 1390 1326 665 631 605 122 107
71
BglII A'GATC_T
Cuts at: 6437 6437
Size: 6442
BlpI GC'TnA_GC
Cuts at: 3859 3859
Size: 6442
Bme1580I G_kGCm'C
Cuts at: 1790 2228 2294 2366 2372 2951 3066 3635 4503
Size: 438 66 72 6 579 115 569 868
Cuts at: 4503 5659 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 868 579 569 438 115 72
66 6
BmgBI CAC'GTC
Cuts at: 1318 2650 3061 1318
Size: 1332 411 4699
Fragments arranged by size:
4699 1332 411
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2747 4173 5131
Size: 416 53 83 399 24 1330 1426 958
Cuts at: 5131 442
Size: 1753
Fragments arranged by size:
1753 1426 1330 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3543 1056
Size: 2487 3955
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2461 2461
Size: 6442
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3131 3452 5102 1239
Size: 1892 321 1650 2579
Fragments arranged by size:
2579 1892 1650 321
Bpu10I CC'TnA_GC
Cuts at: 2273 3355 2273
Size: 1082 5360
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4567 5432 5730 4567
Size: 865 298 5279
Fragments arranged by size:
5279 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3578 5105 882
Size: 617 2079 1527 2219
Fragments arranged by size:
2219 2079 1527 617
BsaAI yAC'GTr
Cuts at: 493 1204 3192 4233 493
Size: 711 1988 1041 2702
Fragments arranged by size:
2702 1988 1041 711
BsaBI GATnn'nnATC
Cuts at: 6436 6436
Size: 6442
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2300 3092 3255 4249
Size: 53 83 186 936 767 792 163 994
Cuts at: 4249 4631 5266 275
Size: 382 635 1451
Fragments arranged by size:
1451 994 936 792 767 635 382 186
163 83 53
BsaWI w'CCGG_w
Cuts at: 2951 4875 5273 5547 5694 6105 2951
Size: 1924 398 274 147 411 3288
Fragments arranged by size:
3288 1924 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6217
Fragments arranged by size:
6217 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2116 2975 1186
Size: 930 859 4653
Fragments arranged by size:
4653 930 859
BseYI C'CCAG_C
Cuts at: 2098 2305 2353 2759 5645 6141 2098
Size: 207 48 406 2886 496 2399
Fragments arranged by size:
2886 2399 496 406 207 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2596 3008 3574 1163
Size: 573 860 412 566 4031
Fragments arranged by size:
4031 860 573 566 412
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2451 3892 4653 5681 1540
Size: 275 186 450 1441 761 1028 2301
Fragments arranged by size:
2301 1441 1028 761 450 275 186
BsiHKAI G_wGCw'C
Cuts at: 2156 2279 2511 2951 2967 3066 4503 4588 5659
Size: 123 232 440 16 99 1437 85 1071
Cuts at: 5659 2156
Size: 2939
Fragments arranged by size:
2939 1437 1071 440 232 123 99 85
16
BsmI GAATG_Cn'
Cuts at: 3953 4046 3953
Size: 93 6349
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2156 2228 2279 2294 2366 2372 2511
Size: 399 366 72 51 15 72 6 139
Cuts at: 2511 2877 2951 2967 3066 3126 3438 3635 3761
Size: 366 74 16 99 60 312 197 126
Cuts at: 3761 4503 4588 5659 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 366 366 312 197
139 126 99 85 74 72 72 60
51 16 15 6
BspEI T'CCGG_A
Cuts at: 6105 6105
Size: 6442
BspHI T'CATG_A
Cuts at: 4331 6061 4331
Size: 1730 4712
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3204 3522 3797 844
Size: 2360 318 275 3489
Fragments arranged by size:
3489 2360 318 275
BsrBI CCG'CTC
Cuts at: 1394 1823 2445 2725 4329 1394
Size: 429 622 280 1604 3507
Fragments arranged by size:
3507 1604 622 429 280
BsrDI GCAATG_nn'
Cuts at: 65 1248 3179 4937 5111 65
Size: 1183 1931 1758 174 1396
Fragments arranged by size:
1931 1758 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2083 2450 2951 3319 5086 5273 1700
Size: 110 273 367 501 368 1767 187 2869
Fragments arranged by size:
2869 1767 501 368 367 273 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6442
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6442
BssSI C'ACGA_G
Cuts at: 2597 4502 5514 2597
Size: 1905 1012 3525
Fragments arranged by size:
3525 1905 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6442
BstBI TT'CG_AA
Cuts at: 5246 5246
Size: 6442
BstEII G'GTnAC_C
Cuts at: 1184 2281 1184
Size: 1097 5345
BstXI CCAn_nnnn'nTGG
Cuts at: 3661 3661
Size: 6442
BstYI r'GATC_y
Cuts at: 1972 2805 2857 3772 4139 4524 4541 5993 6079
Size: 833 52 915 367 385 17 1452 86
Cuts at: 6079 6091 6437 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 915 833 385 367 346 86
52 17 12
Bsu36I CC'TnA_GG
Cuts at: 2107 2107
Size: 6442
BtgI C'CryG_G
Cuts at: 513 1065 3286 3349 3573 3738 513
Size: 552 2221 63 224 165 3217
Fragments arranged by size:
3217 2221 552 224 165 63
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5703
Fragments arranged by size:
5703 683 56
BtsI GCAGTG_nn'
Cuts at: 3958 4752 4772 3958
Size: 794 20 5628
Fragments arranged by size:
5628 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6442
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6407
DraI TTT'AAA
Cuts at: 3746 3746
Size: 6442
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5449 809
Size: 507 4133 1802
Fragments arranged by size:
4133 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2047 2448 3321
Size: 54 1425 50 275 18 217 401 873
Cuts at: 3321 3889 4778 6407 8
Size: 568 889 1629 43
Fragments arranged by size:
1629 1425 889 873 568 401 275 217
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2448 3889 1537
Size: 275 636 1441 4090
Fragments arranged by size:
4090 1441 636 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3503 4372 1353
Size: 2150 869 3423
Fragments arranged by size:
3423 2150 869
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3322 5029 5403 5549 387
Size: 1064 138 1733 1707 374 146 1280
Fragments arranged by size:
1733 1707 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2509 2965 2509
Size: 456 5986
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2789 3131 3452 3753 4505 5102 5889
Size: 393 1157 342 321 301 752 597 787
Cuts at: 5889 6157 1239
Size: 268 1524
Fragments arranged by size:
1524 1157 787 752 597 393 342 321
301 268
EcoO109I rG'GnC_Cy
Cuts at: 2103 2112 2291 2763 3021 3106 3625 3632 3728
Size: 9 179 472 258 85 519 7 96
Cuts at: 3728 2103
Size: 4817
Fragments arranged by size:
4817 519 472 258 179 96 85 9
7
EcoRI G'AATT_C
Cuts at: 3751 4129 3751
Size: 378 6064
FspI TGC'GCA
Cuts at: 4948 4948
Size: 6442
HaeII r_GCGC'y
Cuts at: 1678 2303 2360 3546 5589 1678
Size: 625 57 1186 2043 2531
Fragments arranged by size:
2531 2043 1186 625 57
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2111 2143 2876 2908 2987
Size: 32 135 32 539 32 733 32 79
Cuts at: 2987 3019 3389 3421 5083 5115 5157 5189 1373
Size: 32 370 32 1662 32 42 32 2626
Fragments arranged by size:
2626 1662 733 539 370 135 79 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3136 3786 4032 669
Size: 988 281 1198 650 246 3079
Fragments arranged by size:
3079 1198 988 650 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6442
HpaI GTT'AAC
Cuts at: 4032 4032
Size: 6442
KasI G'GCGC_C
Cuts at: 2299 2299
Size: 6442
KpnI G_GTAC'C
Cuts at: 3767 3767
Size: 6442
MfeI C'AATT_G
Cuts at: 4041 4041
Size: 6442
MluI A'CGCG_T
Cuts at: 6318 6318
Size: 6442
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5556 5740 1305
Size: 4251 184 2007
Fragments arranged by size:
4251 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4620
Fragments arranged by size:
4620 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2795 3005 4400 4759 4918 518
Size: 648 1629 210 1395 359 159 2042
Fragments arranged by size:
2042 1629 1395 648 359 210 159
MspA1I CmG'CkG
Cuts at: 1606 3151 3558 3858 4535 5683 5928 1606
Size: 1545 407 300 677 1148 245 2120
Fragments arranged by size:
2120 1545 1148 677 407 300 245
NaeI GCC'GGC
Cuts at: 2085 2452 3321 2085
Size: 367 869 5206
Fragments arranged by size:
5206 869 367
NarI GG'CG_CC
Cuts at: 2300 2300
Size: 6442
NcoI C'CATG_G
Cuts at: 513 1065 3286 513
Size: 552 2221 3669
Fragments arranged by size:
3669 2221 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6442
NgoMIV G'CCGG_C
Cuts at: 2083 2450 3319 2083
Size: 367 869 5206
Fragments arranged by size:
5206 869 367
NheI G'CTAG_C
Cuts at: 1052 3539 1052
Size: 2487 3955
NotI GC'GGCC_GC
Cuts at: 3889 3889
Size: 6442
NruI TCG'CGA
Cuts at: 3204 3204
Size: 6442
NspI r_CATG'y
Cuts at: 1132 1229 2419 3676 3800 5345 1132
Size: 97 1190 1257 124 1545 2229
Fragments arranged by size:
2229 1545 1257 1190 124 97
PasI CC'CwG_GG
Cuts at: 1348 2287 2335 1348
Size: 939 48 5455
Fragments arranged by size:
5455 939 48
PciI A'CATG_T
Cuts at: 3672 5341 3672
Size: 1669 4773
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2027 1108
Size: 919 5523
PfoI T'CCnGG_A
Cuts at: 2147 2511 3327 3456 2147
Size: 364 816 129 5133
Fragments arranged by size:
5133 816 364 129
PmeI GTTT'AAAC
Cuts at: 3746 3746
Size: 6442
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4511 4543 6024 6056 4511
Size: 32 1481 32 4897
Fragments arranged by size:
4897 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2112 2763 3106 2112
Size: 651 343 5448
Fragments arranged by size:
5448 651 343
PshAI GACnn'nnGTC
Cuts at: 1613 2347 1613
Size: 734 5708
PsiI TTA'TAA
Cuts at: 4012 4012
Size: 6442
PspOMI G'GGCC_C
Cuts at: 2224 2290 2362 2368 3631 2224
Size: 66 72 6 1263 5035
Fragments arranged by size:
5035 1263 72 66 6
PstI C_TGCA'G
Cuts at: 830 2630 3150 3173 3794 830
Size: 1800 520 23 621 3478
Fragments arranged by size:
3478 1800 621 520 23
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6442
SacI G_AGCT'C
Cuts at: 2511 2967 2511
Size: 456 5986
SalI G'TCGA_C
Cuts at: 1936 3784 1936
Size: 1848 4594
SanDI GG'GwC_CC
Cuts at: 2112 2763 2112
Size: 651 5791
SbfI CC_TGCA'GG
Cuts at: 3794 3794
Size: 6442
ScaI AGT'ACT
Cuts at: 4690 6402 4690
Size: 1712 4730
SexAI A'CCwGG_T
Cuts at: 2382 2382
Size: 6442
SfcI C'TryA_G
Cuts at: 826 1045 2626 3146 3169 3790 4925 5606 5797
Size: 219 1581 520 23 621 1135 681 191
Cuts at: 5797 826
Size: 1471
Fragments arranged by size:
1581 1471 1135 681 621 520 219 191
23
SfoI GGC'GCC
Cuts at: 2301 2301
Size: 6442
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6442
SgrAI Cr'CCGG_yG
Cuts at: 1700 2951 1700
Size: 1251 5191
SmaI CCC'GGG
Cuts at: 1872 2229 2367 3630 3637 3726 3769 1872
Size: 357 138 1263 7 89 43 4545
Fragments arranged by size:
4545 1263 357 138 89 43 7
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4546 5447 5709 820
Size: 197 926 2603 901 262 1553
Fragments arranged by size:
2603 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4233 493
Size: 3740 2702
SpeI A'CTAG_T
Cuts at: 152 2344 152
Size: 2192 4250
SphI G_CATG'C
Cuts at: 1132 2419 3800 1132
Size: 1287 1381 3774
Fragments arranged by size:
3774 1381 1287
SrfI GCCC'GGGC
Cuts at: 2229 2367 2229
Size: 138 6304
SspI AAT'ATT
Cuts at: 5 52 4366 5
Size: 47 4314 2081
Fragments arranged by size:
4314 2081 47
StuI AGG'CCT
Cuts at: 1517 2819 1517
Size: 1302 5140
StyI C'CwwG_G
Cuts at: 513 1065 2321 2461 3259 3286 3881 513
Size: 552 1256 140 798 27 595 3074
Fragments arranged by size:
3074 1256 798 595 552 140 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2001 4469 4486 4639 4824 5269 325
Size: 1255 421 2468 17 153 185 445 1498
Fragments arranged by size:
2468 1498 1255 445 421 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 4688 6400
Size: 274 80 33 51 1443 204 2507 1712
Cuts at: 6400 96
Size: 138
Fragments arranged by size:
2507 1712 1443 274 204 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6402 6434 6402
Size: 32 6410
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6442
XbaI T'CTAG_A
Cuts at: 3778 3778
Size: 6442
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6442
XmaI C'CCGG_G
Cuts at: 1870 2227 2365 3628 3635 3724 3767 1870
Size: 357 138 1263 7 89 43 4545
Fragments arranged by size:
4545 1263 357 138 89 43 7
XmnI GAAnn'nnTTC
Cuts at: 2198 4571 2198
Size: 2373 4069
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3256 4250 276
Size: 53 83 186 936 1722 994 2468
Fragments arranged by size:
2468 1722 994 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI SacI SalI SanDI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI
BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI
NsiI PacI PmlI PspXI PsrI PvuII RsrII SacII
SapI SfiI SwaI XcmI