Restriction map for
pFN21AB7880
(Circular) (Six-base) MAPSORT of: pFN21AB7880.seq Check: 4072 from: 1 to: 6625
pFN21AB7880 6625 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4435 278
Size: 53 83 186 936 2899 2468
Fragments arranged by size:
2899 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3968 1937
Size: 2031 4594
Acc65I G'GTAC_C
Cuts at: 3946 3946
Size: 6625
AclI AA'CG_TT
Cuts at: 1563 4752 5125 1563
Size: 3189 373 3063
Fragments arranged by size:
3189 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3539 3601 4688 6072 6340 1632
Size: 1907 62 1087 1384 268 1917
Fragments arranged by size:
1917 1907 1384 1087 268 62
AfeI AGC'GCT
Cuts at: 2019 2019
Size: 6625
AflII C'TTAA_G
Cuts at: 820 1017 3470 820
Size: 197 2453 3975
Fragments arranged by size:
3975 2453 197
AflIII A'CryG_T
Cuts at: 1203 5524 6501 1203
Size: 4321 977 1327
Fragments arranged by size:
4321 1327 977
AgeI A'CCGG_T
Cuts at: 5456 5456
Size: 6625
AhdI GACnn_n'nnGTC
Cuts at: 5354 5354
Size: 6625
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6625
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2522 1184
Size: 1338 5287
AlwNI CAG_nnn'CTG
Cuts at: 3435 4368 5940 3435
Size: 933 1572 4120
Fragments arranged by size:
4120 1572 933
ApaLI G'TGCA_C
Cuts at: 4682 5838 4682
Size: 1156 5469
ApoI r'AATT_y
Cuts at: 1493 2526 2892 3315 3399 3934 4160 4312 6421
Size: 1033 366 423 84 535 226 152 2109
Cuts at: 6421 1493
Size: 1697
Fragments arranged by size:
2109 1697 1033 535 423 366 226 152
84
AseI AT'TA_AT
Cuts at: 160 1032 5179 160
Size: 872 4147 1606
Fragments arranged by size:
4147 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6625
AvaI C'yCGr_G
Cuts at: 1870 1943 3950 1870
Size: 73 2007 4545
Fragments arranged by size:
4545 2007 73
BamHI G'GATC_C
Cuts at: 3955 4322 3955
Size: 367 6258
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2151 2880 3894 3946 5401
Size: 325 217 627 364 729 1014 52 1455
Cuts at: 5401 618
Size: 1842
Fragments arranged by size:
1842 1455 1014 729 627 364 325 217
52
BanII G_rGCy'C
Cuts at: 1391 2948 3924 3944 1391
Size: 1557 976 20 4072
Fragments arranged by size:
4072 1557 976 20
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3607 928
Size: 971 1708 3946
Fragments arranged by size:
3946 1708 971
BbvCI CC'TCA_GC
Cuts at: 2226 2226
Size: 6625
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4816 4850 1903
Size: 34 2879 34 3678
Fragments arranged by size:
3678 2879 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2342 4517 5727 2342
Size: 2175 1210 3240
Fragments arranged by size:
3240 2175 1210
BclI T'GATC_A
Cuts at: 1548 3220 1548
Size: 1672 4953
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5236 136
Size: 107 122 71 631 605 3564 1525
Fragments arranged by size:
3564 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6620 6620
Size: 6625
BlpI GC'TnA_GC
Cuts at: 4042 4042
Size: 6625
Bme1580I G_kGCm'C
Cuts at: 1790 2883 4686 5842 1790
Size: 1093 1803 1156 2573
Fragments arranged by size:
2573 1803 1156 1093
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6625
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4356 5314 442
Size: 416 53 83 399 24 2939 958 1753
Fragments arranged by size:
2939 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6625
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2195 2751 3093 3177 3345 3429 5285 1239
Size: 956 556 342 84 168 84 1856 2579
Fragments arranged by size:
2579 1856 956 556 342 168 84 84
Bpu10I CC'TnA_GC
Cuts at: 2226 2226
Size: 6625
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2962 3323 3560 3659 4750 5615 5913 2962
Size: 361 237 99 1091 865 298 3674
Fragments arranged by size:
3674 1091 865 361 298 237 99
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2642 5288 882
Size: 617 1143 2646 2219
Fragments arranged by size:
2646 2219 1143 617
BsaAI yAC'GTr
Cuts at: 493 1204 4416 493
Size: 711 3212 2702
Fragments arranged by size:
3212 2702 711
BsaBI GATnn'nnATC
Cuts at: 2330 6619 2330
Size: 4289 2336
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4432 4814 5449 275
Size: 53 83 186 936 2899 382 635 1451
Fragments arranged by size:
2899 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3827 5058 5456 5730 5877 6288 3827
Size: 1231 398 274 147 411 4164
Fragments arranged by size:
4164 1231 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6400
Fragments arranged by size:
6400 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2135 2243 2507 1186
Size: 949 108 264 5304
Fragments arranged by size:
5304 949 264 108
BseYI C'CCAG_C
Cuts at: 5828 6324 5828
Size: 496 6129
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3196 3700 3748 1163
Size: 573 1460 504 48 4040
Fragments arranged by size:
4040 1460 573 504 48
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4075 4836 5864 1540
Size: 275 186 2074 761 1028 2301
Fragments arranged by size:
2301 2074 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2948 3309 3645 4686 4771 5842 2948
Size: 361 336 1041 85 1071 3731
Fragments arranged by size:
3731 1071 1041 361 336 85
BsmI GAATG_Cn'
Cuts at: 3132 3431 3515 4136 4229 3132
Size: 299 84 621 93 5528
Fragments arranged by size:
5528 621 299 93 84
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2883 2948 3309 3645 3924 3944 4686
Size: 399 1093 65 361 336 279 20 742
Cuts at: 4686 4771 5842 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1093 1071 742 399 361 336 279
85 65 20
BspEI T'CCGG_A
Cuts at: 6288 6288
Size: 6625
BspHI T'CATG_A
Cuts at: 2161 2340 4514 6244 2161
Size: 179 2174 1730 2542
Fragments arranged by size:
2542 2174 1730 179
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2451 3980 844
Size: 1607 1529 3489
Fragments arranged by size:
3489 1607 1529
BsrBI CCG'CTC
Cuts at: 1394 1823 3372 4512 1394
Size: 429 1549 1140 3507
Fragments arranged by size:
3507 1549 1140 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3013 3604 5120 5294 65
Size: 1183 1765 591 1516 174 1396
Fragments arranged by size:
1765 1516 1396 1183 591 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5269 5456 1700
Size: 110 3459 187 2869
Fragments arranged by size:
3459 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6625
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6625
BssSI C'ACGA_G
Cuts at: 4685 5697 4685
Size: 1012 5613
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6625
BstBI TT'CG_AA
Cuts at: 5429 5429
Size: 6625
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6625
BstXI CCAn_nnnn'nTGG
Cuts at: 2468 2468
Size: 6625
BstYI r'GATC_y
Cuts at: 1972 3955 4322 4707 4724 6176 6262 6274 6620
Size: 1983 367 385 17 1452 86 12 346
Cuts at: 6620 1972
Size: 1977
Fragments arranged by size:
1983 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2043 513
Size: 552 939 39 5095
Fragments arranged by size:
5095 939 552 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5886
Fragments arranged by size:
5886 683 56
BtsI GCAGTG_nn'
Cuts at: 2506 3184 3436 3520 3688 3765 4141 4935 4955
Size: 678 252 84 168 77 376 794 20
Cuts at: 4955 2506
Size: 4176
Fragments arranged by size:
4176 794 678 376 252 168 84 77
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6625
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2824 2859 2995 3030 3250 3285 3586
Size: 35 2260 35 136 35 220 35 301
Cuts at: 3586 3621 529
Size: 35 3533
Fragments arranged by size:
3533 2260 301 220 136 35 35 35
35 35
DraI TTT'AAA
Cuts at: 3929 3929
Size: 6625
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5632 809
Size: 507 4316 1802
Fragments arranged by size:
4316 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2409 3049
Size: 54 1425 50 275 18 177 402 640
Cuts at: 3049 4072 4961 6590 8
Size: 1023 889 1629 43
Fragments arranged by size:
1629 1425 1023 889 640 402 275 177
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4072 1537
Size: 275 2260 4090
Fragments arranged by size:
4090 2260 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2049 3912 4555 1353
Size: 696 1863 643 3423
Fragments arranged by size:
3423 1863 696 643
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3017 5212 5586 5732 387
Size: 1064 138 1428 2195 374 146 1280
Fragments arranged by size:
2195 1428 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2946 2946
Size: 6625
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2195 2751 3093 3177 3345 3429 3539
Size: 393 563 556 342 84 168 84 110
Cuts at: 3539 3601 4688 5285 6072 6340 1239
Size: 62 1087 597 787 268 1524
Fragments arranged by size:
1524 1087 787 597 563 556 393 342
268 168 110 84 84 62
EcoO109I rG'GnC_Cy
Cuts at: 2128 2233 2294 2128
Size: 105 61 6459
Fragments arranged by size:
6459 105 61
EcoRI G'AATT_C
Cuts at: 2892 3315 3399 3934 4312 2892
Size: 423 84 535 378 5205
Fragments arranged by size:
5205 535 423 378 84
FspI TGC'GCA
Cuts at: 2259 5131 2259
Size: 2872 3753
HaeII r_GCGC'y
Cuts at: 1678 2021 5772 1678
Size: 343 3751 2531
Fragments arranged by size:
3751 2531 343
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5266 5298 5340 5372 1373
Size: 32 135 32 3694 32 42 32 2626
Fragments arranged by size:
3694 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3969 4215 669
Size: 988 281 2031 246 3079
Fragments arranged by size:
3079 2031 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2739 748
Size: 1991 4634
HpaI GTT'AAC
Cuts at: 4215 4215
Size: 6625
KpnI G_GTAC'C
Cuts at: 3950 3950
Size: 6625
MfeI C'AATT_G
Cuts at: 4224 4224
Size: 6625
MluI A'CGCG_T
Cuts at: 6501 6501
Size: 6625
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2603 2623 5739 5923 1305
Size: 1298 20 3116 184 2007
Fragments arranged by size:
3116 2007 1298 184 20
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2411 3051 10
Size: 54 1425 343 579 640 3584
Fragments arranged by size:
3584 1425 640 579 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2319 3123 4583 4942 5101 518
Size: 648 1153 804 1460 359 159 2042
Fragments arranged by size:
2042 1460 1153 804 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4041 4718 5866 6111 1606
Size: 2435 677 1148 245 2120
Fragments arranged by size:
2435 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2043 513
Size: 552 939 39 5095
Fragments arranged by size:
5095 939 552 39
NdeI CA'TA_TG
Cuts at: 387 3350 387
Size: 2963 3662
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6625
NotI GC'GGCC_GC
Cuts at: 4072 4072
Size: 6625
NspI r_CATG'y
Cuts at: 1132 1229 3388 3774 3983 5528 1132
Size: 97 2159 386 209 1545 2229
Fragments arranged by size:
2229 2159 1545 386 209 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6625
PciI A'CATG_T
Cuts at: 5524 5524
Size: 6625
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6625
PmeI GTTT'AAAC
Cuts at: 3929 3929
Size: 6625
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4694 4726 6207 6239 4694
Size: 32 1481 32 5080
Fragments arranged by size:
5080 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2294 2294
Size: 6625
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6625
PsiI TTA'TAA
Cuts at: 2745 3759 4195 2745
Size: 1014 436 5175
Fragments arranged by size:
5175 1014 436
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2765 2797 2765
Size: 32 6593
PstI C_TGCA'G
Cuts at: 830 3977 830
Size: 3147 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6625
SacI G_AGCT'C
Cuts at: 2948 2948
Size: 6625
SalI G'TCGA_C
Cuts at: 1936 3967 1936
Size: 2031 4594
SapI GCTCTTCn'nnn_
Cuts at: 3912 3912
Size: 6625
SbfI CC_TGCA'GG
Cuts at: 3977 3977
Size: 6625
ScaI AGT'ACT
Cuts at: 4873 6585 4873
Size: 1712 4913
SfcI C'TryA_G
Cuts at: 826 1045 3973 5108 5789 5980 826
Size: 219 2928 1135 681 191 1471
Fragments arranged by size:
2928 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6625
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6625
SmaI CCC'GGG
Cuts at: 1872 3952 1872
Size: 2080 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2941 3302 3470 3539 3638 4729
Size: 197 926 998 361 168 69 99 1091
Cuts at: 4729 5630 5892 820
Size: 901 262 1553
Fragments arranged by size:
1553 1091 998 926 901 361 262 197
168 99 69
SnaBI TAC'GTA
Cuts at: 493 4416 493
Size: 3923 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6625
SphI G_CATG'C
Cuts at: 1132 3983 1132
Size: 2851 3774
SspI AAT'ATT
Cuts at: 5 52 4549 5
Size: 47 4497 2081
Fragments arranged by size:
4497 2081 47
StuI AGG'CCT
Cuts at: 1517 3082 3250 3278 3502 3754 3782 1517
Size: 1565 168 28 224 252 28 4360
Fragments arranged by size:
4360 1565 252 224 168 28 28
StyI C'CwwG_G
Cuts at: 513 1065 2004 2043 4064 513
Size: 552 939 39 2021 3074
Fragments arranged by size:
3074 2021 939 552 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2627 4652 4669 4822 5007 5452 325
Size: 1255 1047 2025 17 153 185 445 1498
Fragments arranged by size:
2025 1498 1255 1047 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2106 4871 6583
Size: 274 80 33 51 1443 129 2765 1712
Cuts at: 6583 96
Size: 138
Fragments arranged by size:
2765 1712 1443 274 138 129 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6585 6617 6585
Size: 32 6593
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6625
XbaI T'CTAG_A
Cuts at: 3961 3961
Size: 6625
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6625
XmaI C'CCGG_G
Cuts at: 1870 3950 1870
Size: 2080 4545
XmnI GAAnn'nnTTC
Cuts at: 2852 3023 3194 3278 3446 3866 4754 2852
Size: 171 171 84 168 420 888 4723
Fragments arranged by size:
4723 888 420 171 171 168 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4433 276
Size: 53 83 186 936 2899 2468
Fragments arranged by size:
2899 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PsrI PstI PvuI SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PvuII RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI